Habitat and scale shape the demographic fate of the keystone sea urchin <i>Paracentrotus lividus</i> in Mediterranean macrophyte communities

Demographic processes exert different degrees of control as individuals grow, and in species that span several habitats and spatial scales, this can influence our ability to predict their population at a particular life-history stage given the previous life stage. In particular, when keystone species are involved, this relative coupling between demographic stages can have significant implications for the functioning of ecosystems. We examined benthic and pelagic abundances of the sea urchin Paracentrotus lividus in order to: 1) understand the main life-history bottlenecks by observing the degree of coupling between demographic stages; and 2) explore the processes driving these linkages. P. lividus is the dominant invertebrate herbivore in the Mediterranean Sea, and has been repeatedly observed to overgraze shallow beds of the seagrass Posidonia oceanica and rocky macroalgal communities. We used a hierarchical sampling design at different spatial scales (100 s, 10 s and &lt;1 km) and habitats (seagrass and rocky macroalgae) to describe the spatial patterns in the abundance of different demographic stages (larvae, settlers, recruits and adults). Our results indicate that large-scale factors (potentially currents, nutrients, temperature, etc.) determine larval availability and settlement in the pelagic stages of urchin life history. In rocky macroalgal habitats, benthic processes (like predation) acting at large or medium scales drive adult abundances. In contrast, adult numbers in seagrass meadows are most likely influenced by factors like local migration (from adjoining rocky habitats) functioning at much smaller scales. The complexity of spatial and habitat-dependent processes shaping urchin populations demands a multiplicity of approaches when addressing habitat conservation actions, yet such actions are currently mostly aimed at managing predation processes and fish numbers. We argue that a more holistic ecosystem management also needs to incorporate the landscape and habitat-quality level processes (eutrophication, fragmentation, etc.) that together regulate the populations of this keystone herbivore

The dyad partnership-credibility and the qualification of suppliers

The need to improve relations between company and supplier suggests that the dyad partnership and credibility must be enhanced in order to maximize gains for both parties that would otherwise not take place. This study demonstrates that companies and suppliers feel the need to review the results achieved by both parties and to influence the way they treat their relationships in the supply of goods and services. This study highlights the need to explore two aspects: firstly, the focus of the importance of supplier partnership for improvement of the company and, secondly, the need to create links based in credibility for the improvement of relations between company and supplier, in order to establish a win-win process in their purchasing and supplying activities. This research applied a questionnaire to 154 elements of various industrial segments. The results confirm the widespread perception on the part of elements of the companies of the need for deepening the partnership between company and supplier, but it verified, however, less openness with regard to the sharing of knowledge, which can be an obstacle to the realization of this deepening. The analysis is done under the perspective of the qualification of suppliers

Mechanistic and Crystallographic Studies of Azoreductase AzoA from Bacillus wakoensis A01

The azoreductase AzoA from the alkali-tolerant Bacillus wakoensis A01 has been studied to reveal its structural and mechanistic details. For this, a recombinant expression system was developed which yields impressive amounts of fully active enzyme. The purified holo enzyme is remarkably solvent-tolerant and thermostable with an apparent melting temperature of 71 °C. The dimeric enzyme contains FMN as a prosthetic group and is strictly NADH dependent. While AzoA shows a negligible ability to use molecular oxygen as an electron acceptor, it is efficient in reducing various azo dyes and quinones. The kinetic and catalytic mechanism has been studied in detail using steady state kinetic analyses and stopped-flow studies. The data show that AzoA performs quinone and azo dye reductions via a two-electron transfer. Moreover, quinones were shown to be much better substrates (kcat values of 100-400 s-1 for several naphtoquinones) when compared with azo dyes. This suggests that the physiological role of AzoA and sequence-related microbial reductases is linked to quinone reductions and that they can better be annotated as quinone reductases. The structure of AzoA has been determined in complex with FMN at 1.8 Å resolution. AzoA displays unique features in the active site providing clues for explaining its catalytic and thermostability features. An uncommon loop, when compared with sequence-related reductases, forms an active site lid with Trp60 acting as an anchor. Several Trp60 mutants have been analyzed disclosing an important role of this residue in the stability of AzoA, while they retained activity. Structural details are discussed in relation to other azo and quinone reductases. This study provides new insights into the molecular functioning of AzoA and sequence-related reductases

Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

A multigene phylogeny was constructed, including a significant number of representative species of the main lineages in the Xylariaceae and four DNA loci the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, the second largest subunit of the RNA polymerase II (RPB2), and beta-tubulin (TUB2). Specimens were selected based on more than a decade of intensive morphological and chemotaxonomic work, and cautious taxon sampling was performed to cover the major lineages of the Xylariaceae; however, with emphasis on hypoxyloid species. The comprehensive phylogenetic analysis revealed a clear-cut segregation of the Xylariaceae into several major clades, which was well in accordance with previously established morphological and chemotaxonomic concepts. One of these clades contained Annulohypoxylon, Hypoxylon, Daldinia, and other related genera that have stromatal pigments and a nodulisporium-like anamorph. They are accommodated in the family Hypoxylaceae, which is resurrected and emended. Representatives of genera with a nodulisporium-like anamorph and bipartite stromata, lacking stromatal pigments (i.e. Biscogniauxia, Camillea, and Obolarina) appeared in a clade basal to the xylarioid taxa. As they clustered with Graphostroma platystomum, they are accommodated in the Graphostromataceae. The new genus Jackrogersella with J. multiformis as type species is segregated from Annulohypoxylon. The genus Pyrenopolyporus is resurrected for Hypoxylon polyporus and allied species. The genus Daldinia and its allies Entonaema, Rhopalostroma, Ruwenzoria, and Thamnomyces appeared in two separate subclades, which may warrant further splitting of Daldinia in the future, and even Hypoxylon was divided in several clades. However, more species of these genera need to be studied before a conclusive taxonomic rearrangement can be envisaged. Epitypes were designated for several important species in which living cultures and molecular data are available, in order to stabilise the taxonomy of the Xylariales.Fil: Wendt, Lucile. Helmholtz-Zentrum für Infektionsforschung GmbH. Department of Microbial Drugs; Alemania. German Centre for Infection Research; AlemaniaFil: Sir, Esteban Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Unidad Ejecutora Lillo. Fundación Miguel Lillo. Unidad Ejecutora Lillo; ArgentinaFil: Kuhnert, Eric. Helmholtz-Zentrum für Infektionsforschung GmbH. Department of Microbial Drugs; Alemania. German Centre for Infection Research; AlemaniaFil: Heitkämper, Simone. Helmholtz-Zentrum für Infektionsforschung GmbH. Department of Microbial Drugs; Alemania. German Centre for Infection Research; AlemaniaFil: Lambert, Christopher. Helmholtz-Zentrum für Infektionsforschung GmbH. Department of Microbial Drugs; Alemania. German Centre for Infection Research; AlemaniaFil: Hladki, Adriana I.. Fundación Miguel Lillo. Dirección de Botánica. Instituto de Micologia; ArgentinaFil: Romero, Andrea Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Luangsa-Ard, Janet Jennifer. National Center for Genetic Engineering and Biotechnology; TailandiaFil: Srikitikulchai, Prasert. National Center for Genetic Engineering and Biotechnology; TailandiaFil: Peršoh, Derek. Ruhr-Universität Bochum; AlemaniaFil: Stadler, Marc. Helmholtz-Zentrum für Infektionsforschung GmbH. Department of Microbial Drugs; Alemania. German Centre for Infection Research; Alemani

The effect of a centenary storm on the long-lived seagrass Posidonia oceanica

We used the disturbance resulting from a once in a 100‐yr storm on the northwest Mediterranean coast to examine the extent of the disturbance, the tolerance thresholds to burial, and the medium‐term response of the long‐lived Posidonia oceanica seagrass. Sediment burial at 12 surveyed areas was particularly strong in shallow meadows, with 23% of their surfaces buried, on average, under more than 10 cm of sediment. In contrast, less than 5% of the meadow was affected at deeper locations. At three sites, we tracked short‐term mortality along a gradient of sediment burial. Survival response to burial was clearly nonlinear, with a significant threshold at 4-5 cm, beyond which shoot mortality was 100%. To track medium‐term potential recovery, we established permanent plots subject to three sediment burial levels (0-5, 5-10, and > 10 cm burial) in four meadows. Where the initial shoot mortality was 100%, we recorded no shoot recovery over the 4‐yr period. In the remaining plots, where some shoots remained alive, we detected either further mortality or shoot recovery of 7% per year on average. Extreme storm events can result in sudden catastrophic losses of seagrass cover in shallow P. oceanica meadows. In the long term and due to the long return time of such storms, the species may still be able to recover despite its low recovery potential. However, added anthropogenic stressors, including climate change, may seriously test the ability of long‐lived shallow seagrass ecosystems to resist high‐intensity natural disturbances and may be critical for its persistence

Detecting the impacts of harbour construction on a seagrass habitat and its subsequent recovery

Managing coastal development requires a set of tools to adequately detect ecosystem and water column degradation, but it also demands tools to detect any post-disturbance improvement. Structural seagrass indicators (such as shoot density or cover) are often used to detect or assess disturbances, but while they may be very sensitive to the impact itself, it is unclear if those indicators on their own can effectively reflect recovery at time scales relevant to managers. We used the construction of a harbour affecting a nearby Posidonia oceanica seagrass community to test the ability of a set of indicators (structural and others) to detect alterations and to evaluate their sensitivity to recovery of environmental quality after harbour construction was complete and the disturbance ceased. We used a Beyond Before After Control Impact (BBACI) design to evaluate effects on one impacted and three control meadows where we used structural, morphological, community and physiological indicators (26 in total) to asses disturbance impacts. Additionally, we measured some of the potential environmental factors that could be altered during and after the construction of the harbour and are critical to the survival of the seagrass meadow (light, sediment organic matter, sediment accrual). Harbour construction caused a clear increase in sediment organic matter and in sediment deposition rates, especially fine sand. Light availability was also reduced due to suspended sediments. Sediment and light conditions returned to normal levels 5 and 15 months after the construction began. As expected, seagrass structural indicators responded unequivocally to these environmental changes, with clear reductions in shoot density. Additionally, reduced light conditions quickly resulted in a decline in carbohydrate content in affected meadows. Unexpectedly, we also recorded a significant increase in metal content in plant tissues. No response was detected in the physiological indicators related to eutrophication (e.g. N and P content in tissues) and in morphological (shoot biomass) and community (epiphyte biomass) indicators. More than three years after the completion of the harbour, structural indicators did not show any sign of recovery. In contrast, physiological indicators, mainly heavy metal and carbohydrates content, were much better in detecting the improvement of the environmental conditions over the fairly short period of this study. These results indicate that while structural indicators are critical to evaluate the immediate effect of disturbances and the recovery on impacted systems, specific physiological indicators may be much better suited to determining the timing of environmental quality recovery. The design of impact and monitoring protocols in the wake of coastal developmental projects need to consider the differential effectiveness and time-response of measured indicators carefully

Three-dimensional architecture and biogenesis of membrane structures associated with hepatitis C virus replication

All positive strand RNA viruses are known to replicate their genomes in close association with intracellular membranes. In case of the hepatitis C virus (HCV), a member of the family Flaviviridae, infected cells contain accumulations of vesicles forming a membranous web (MW) that is thought to be the site of viral RNA replication. However, little is known about the biogenesis and three-dimensional structure of the MW. In this study we used a combination of immunofluorescence- and electron microscopy (EM)-based methods to analyze the membranous structures induced by HCV in infected cells. We found that the MW is derived primarily from the endoplasmic reticulum (ER) and contains markers of rough ER as well as markers of early and late endosomes, COP vesicles, mitochondria and lipid droplets (LDs). The main constituents of the MW are single and double membrane vesicles (DMVs). The latter predominate and the kinetic of their appearance correlates with kinetics of viral RNA replication. DMVs are induced primarily by NS5A whereas NS4B induces single membrane vesicles arguing that MW formation requires the concerted action of several HCV replicase proteins. Three-dimensional reconstructions identify DMVs as protrusions from the ER membrane into the cytosol, frequently connected to the ER membrane via a neck-like structure. In addition, late in infection multi-membrane vesicles become evident, presumably as a result of a stress-induced reaction. Thus, the morphology of the membranous rearrangements induced in HCV-infected cells resemble those of the unrelated picorna-, corona- and arteriviruses, but are clearly distinct from those of the closely related flaviviruses. These results reveal unexpected similarities between HCV and distantly related positive-strand RNA viruses presumably reflecting similarities in cellular pathways exploited by these viruses to establish their membranous replication factories

The census of interstellar complex organic molecules in the Class I hot corino of SVS13-A

We present the first census of the interstellar Complex Organic Molecules (iCOMs) in the low-mass Class I protostar SVS13-A, obtained by analysing data from the IRAM-30m Large Project ASAI (Astrochemical Surveys At IRAM). They consist of an high-sensitivity unbiased spectral survey at the 1mm, 2mm and 3mm IRAM bands. We detected five iCOMs: acetaldehyde (CH$_3$CHO), methyl formate (HCOOCH$_3$), dimethyl ether (CH$_3$OCH$_3$), ethanol (CH$_3$CH$_2$OH) and formamide (NH$_2$CHO). In addition we searched for other iCOMs and ketene (H$_2$CCO), formic acid (HCOOH) and methoxy (CH$_3$O), whose only ketene was detected. The numerous detected lines, from 5 to 37 depending on the species, cover a large upper level energy range, between 15 and 254 K. This allowed us to carry out a rotational diagram analysis and derive rotational temperatures between 35 and 110 K, and column densities between $3\times 10^{15}$ and $1\times 10^{17}$ cm$^{-2}$ on the 0."3 size previously determined by interferometric observations of glycolaldehyde. These new observations clearly demonstrate the presence of a rich chemistry in the hot corino towards SVS13-A. The measured iCOMs abundances were compared to other Class 0 and I hot corinos, as well as comets, previously published in the literature. We find evidence that (i) SVS13-A is as chemically rich as younger Class 0 protostars, and (ii) the iCOMs relative abundances do not substantially evolve during the protostellar phase.Comment: 24 pages, MNRAS in pres

Health-related quality of life and leisure physical activity during pregnancy

The practice of physical activity during pregnancy plays an important role related to improvement how women perceive their health status. The aim of this study was to investigate the relationship between perceived quality of life during pregnancy and the practice of physical activity during leisure time. It is a cross-sectional study of 187 postpartum women. A retrospective questionnaire was administered to obtain information regarding pregnancy, being evaluated the pattern of physical activity and health status. To evaluate the aspects related to quality of life we used the SF-12 questionnaire. The statistical analysis were t-test, chi-square and multiple linear regression analysis. From the results it was observed that mothers who were active during pregnancy considered their good or excellent health compared do non active (p=0.005, 35.9% and 15.5% respectively). Active women had higher mental component when compared to those who did not practice (p=0.035). There was a relationship between mental component with the private health service, income, education level, disease and presence of a partner. The physical component was related to maternal age, low birth weight, obesity, and smoking during pregnancy. Despite univariate analysis showed a positive association between physical activity during leisure time and the mental component of quality of life related to health, this association lost significance when included age and care health sectors. Longitudinal studies should be developed to measure effects of physical activity in quality of life

Differences in predator composition alter the direction of structure‐mediated predation risk in macrophyte communities

Structural complexity strongly influences the outcome of predator-prey interactions in benthic marine communities affecting both prey concealment and predator hunting efficacy. How habitat structure interacts with species‐specific differences in predatory style and antipredatory strategies may therefore be critical in determining higher trophic functions. We examined the role of structural complexity in mediating predator-prey interactions across several macrophyte habitats along a gradient of structural complexity in three different bioregions: western Mediterranean Sea (WMS), eastern Indian Ocean (EIO) and northern Gulf of Mexico (NGM). Using sea urchins as model prey, we measured survival rates of small (juveniles) and medium (young adults) size classes in different habitat zones: within the macrophyte habitat, along the edge and in bare sandy spaces. At each site we also measured structural variables and predator abundance. Generalised linear models identified biomass and predatory fish abundance as the main determinants of predation intensity but the efficiency of predation was also influenced by urchin size class. Interestingly though, the direction of structure‐mediated effects on predation risk was markedly different between habitats and bioregions. In WMS and NGM, where predation by roving fish was relatively high, structure served as a critical prey refuge, particularly for juvenile urchins. In contrast, in EIO, where roving fish predation was low, predation was generally higher inside structurally complex environments where sea stars were responsible for much of the predation. Larger prey were generally less affected by predation in all habitats, probably due to the absence of large predators. Overall, our results indicate that, while the structural complexity of habitats is critical in mediating predator-prey interactions, the direction of this mediation is strongly influenced by differences in predator composition. Whether the regional pool of predators is dominated by visual roving species or chemotactic benthic predators may determine if structure dampens or enhances the influence of top-down control in marine macrophyte communities