Fertirrigação nitrogenada para produção de macieiras Eva e Princesa em Petrolina-PE.

O presente trabalho foi desenvolvido com o objetivo de avaliar produção de frutos das 26 macieiras ?Eva? e ?Princesa?, em função a fertirrigação nitrogenada em Petrolina-PE

Biometrical, biochemical and molecular diagnosis of Portuguese Meloidogyne hispanica isolates

Meloidogyne hispanica infects many economically important crops worldwide. The accurate identification of this pathogen is essential for the establishment of efficient and sustainable integrated pest management programs. Portuguese M. hispanica isolates were studied by biometrical, biochemical, and molecular characteristics. Biometrical characteristics of M. hispanica females, males, and second-stage juveniles were similar to the original description. Biochemical studies revealed a unique enzyme pattern (Hi4) for M. hispanica esterases that allowed for species differentiation. Molecular analysis of the mtDNA region from COII and 16S rRNA genes resulted in amplification products (1,800 bp) similar to M. hispanica, M. ethiopica, and M. javanica, and the described HinfI was unable to discriminate M. hispanica from the other two species. Analysis of the mtDNA sequences revealed altered nucleotides among the isolates that created new restriction sites for AluI and DraIII. The resulting restriction patterns successfully discriminated between the three species, providing a new tool for Meloidogyne identification. Finally, the phylogenetic relationship between M. hispanica and several Meloidogyne spp. sequences was analyzed using mtDNA, confirming the divergence between meiotic and mitotic species and revealing the proximity of M. hispanica to closely related species. Based on the studies conducted, the application of isozyme or polymerase chain reaction restriction fragment length polymorphism analysis would be a useful and efficient methodology for M. hispanica identification. </jats:p

Advances in the phylogenetic study of the tribe Cinchoneae (Rubiaceae) with emphasis on the genus Cinchona and Ladenbergia

In its current circumscription, the Cinchoneae tribe includes 9 genera of neotropical distribution. Its species occur mostly in the Andes in South America, with few reaching Central America. Preliminary phylogenetic studies have suggested the monophyly of Cinchoneae but intrageneric relationships are still debatable. In this study, we sampled 8 genera of Cinchoneae (with ca 50% of species for Cinchona and Ladenbergia) and obtained multiple single-copy nuclear loci (ca 207 genes) by using the “Angiosperm353 universal probe set”, which was complemented with a taxonomic review of Cinchoneae. Phylogenetic inferences were realized with multispecies pseudo-coalescent (ASTRAL III) and gene concatenation analysis (ML). Our results strongly support the monophyly of the tribe and most of the genera, except for Ladenbergia. Furthermore, Ciliosemina, Ladenbergia, and Remijia formed a clade, although the position of Ciliosemina (= Remijia pedunculata) and Ladenbergia muzonensis is still elusive. The position of Ladenbergia muzonensis is intriguing due to its intermedíate floral morphology, which resembles both Remijia and Ladenbergia species. Additionally, our phylogeny also supports the recognition of a new species in Cinchona. Finally, our results show that sequencing data using the probe set designed for multiple gene capture is a useful tool for phylogenetic reconstructions in taxonomically complex groups

POLYBIUS2020, a cost-effective underwater autonomous video system to record fishing gear selectivity performance catching fish and marine litter

Underwater video cameras are a highly versatile survey solution for marine fisheries research. The POLYBIUS2020 is a system specially designed to be used as a tool for video recording inside towed fishing gears. Its design allows for rapid installation onboard commercial fishing vessels as well as for quick reconfiguration and battery replacement. The system is based on simple commercial components to ensure low costs and the opportunity of future studies using house technology. The field experiments carried out have shown the flexibility and ability of the system to obtain key information about fishing selectivity, flora and fauna characterization and marine litter presence

Dedicated versus mainstreaming approaches in local climate plans in Europe

Cities are gaining prominence committing to respond to the threat of climate change, e.g., by developing local climate plans or strategies. However, little is known regarding the approaches and processes of plan development and implementation, or the success and effectiveness of proposed measures. Mainstreaming is regarded as one approach associated with (implementation) success, but the extent of integration of local climate policies and plans in ongoing sectoral and/or development planning is unclear. This paper analyses 885 cities across the 28 European countries to create a first reference baseline on the degree of climate mainstreaming in local climate plans. This will help to compare the benefits of mainstreaming versus dedicated climate plans, looking at policy effectiveness and ultimately delivery of much needed climate change efforts at the city level. All core cities of the European Urban Audit sample were analyzed, and their local climate plans classified as dedicated or mainstreamed in other local policy initiatives. It was found that the degree of mainstreaming is low for mitigation (9% of reviewed cities; 12% of the identified plans) and somewhat higher for adaptation (10% of cities; 29% of plans). In particular horizontal mainstreaming is a major effort for local authorities; an effort that does not necessarily pay off in terms of success of action implementation. This study concludes that climate change issues in local municipalities are best tackled by either, developing a dedicated local climate plan in parallel to a mainstreamed plan or by subsequently developing first the dedicated and later a mainstreaming plan (joint or subsequent “dual track approach”). Cities that currently provide dedicated local climate plans (66% of cities for mitigation; 26% of cities for adaptation) may follow-up with a mainstreaming approach. This promises effective implementation of tangible climate actions as well as subsequent diffusion of climate issues into other local sector policies. The development of only broad sustainability or resilience strategies is seen as critical.We thank the many council representatives that supported the datacollection. Special thanks to Birgit Georgi who helped in setting up this large net work of researchers across the EU-28. We also thank the EU COST Action TU 0902 (ledbyRichardDawson) that established the core research network and the positive engagement and interaction of th emembers of this group. OH is Fellow of the Tyndall Centre for Climate Change Research and was funded by the UK EPSRC LC Transforms: Low Carbon Transitions of Fleet Operations in Metropolitan Sites Project (grant number EP/N010612/1). EKL was supported by the Ministry of Education, Youth and Sports, Czechia, within the National Sustainability Program I (NPU I) (grant number LO1415). DG ac-knowledges support by the Ministry of Education, University and Research (MIUR), Italy ("Departments of Excellence" grant L. 232/2016). HO was supported by the Ministry of Education and Research, Estonia (grantnumberIUT34-17). MO acknowledges funding from the Ministry of Economy and Competitiveness (MINECO), Spain (grant number IJCI-2016-28835). SS acknowledges that CENSE's research is partially funded by the Science Foundation, Portugal (grant number UID/AMB/04085/2019). The paper reflects only the views of the authors. The European Union, the European Environment Agency or other supporting bodies are not liable for any use that may be made of the information that is provided in this manuscript

Formas de aplicação de bioestimulante na produção da cana-de-açúcar.

Devido a competitividade da cana-de-açúcar irrigada e ao elevado custo para o cultivo, o manejo eficiente torna-se um fator preponderante para sustentabilidade das propriedades. O objetivo deste trabalho foi avaliar o efeito das formas de aplicação de bioestimulante no crescimento e na qualidade tecnológica da cana-de-açúcar no semiárido brasileiro. O experimento foi conduzido em viveiro aberto, localizado na Universidade Federal do Vale do São Francisco, campus Juazeiro-BA. O delineamento experimental foi em blocos casualizados, dispostos em esquema fatorial 3 x 5, sendo três formas de aplicação do bioestimulante: a) Via pulverização do colmo nas covas de plantio, b) Via sistema de irrigação tipo gotejamento e c) Via pulverização foliar, nas primeiras emergências foliares; e cinco doses de bioestimulante Stimulate®: 0; 0,3; 0,6; 0,9 e 1,2 L ha -1 , com 6 repetições. Foram avaliados os parâmetros referentes à morfologia das plantas e as variáveis tecnológicas: teor de sólidos solúveis; teor de sacarose; fibra industrial na cana-de-açúcar; pureza do caldo; percentagem de açúcar aparente; açúcares totais recuperáveis. Apesar da aplicação de bioestimulante na dose de 0,82 L ha-1 promover melhor produção de massa de colmos, as três formas de aplicação não foram efetivas no aumento na qualidade tecnológica da cana-de-açúcar