Onward to the Mid-Atlantic: First records of Cuban Brown Anoles (Anolis sagrei) on Ascension Island

Cuban Brown Anoles (Anolis sagrei) are native to Cuba and many Bahamian islands (Schwartz and Henderson1991), but the species has an expansive non-native range, having been introduced and become established in a number of US states (Florida, Georgia, Louisiana, Texas, California), several Lesser Antillean islands (Grenada, St. Vincent, the Grenadines, Barbados), Mexico, Belize, Hawaii, Bermuda (Stroud et al. 2017), Taiwan, and Singapore (reviewed in Henderson and Powell 2009). Recently, two newly discovered populations represent expansion of A. sagrei into the Southern Hemisphere for the first time, first in the Pacific coastal town of Guayaquil, Ecuador (Amador et al. 2017) and secondly in Rio de Janeiro, Brazil (Oliveira et al. 2018). Herein we present further evidence of A. sagrei in the Southern Hemisphere, with the first record of A. sagrei on the mid-Atlantic island of Ascension (Fig. 1A). No native terrestrial reptiles occur on Ascension, but four other species of lizards have been introduced and are established (Edgar 2010): Asian House Gecko (Hemidactylus frenatus), Gray’s Leaf-toed Gecko (H. mercatorius), Cradock Thick-toed Gecko (Pachydactylis geitje), and Weigmann’s Swift (Liolaemus wiegmanni)

Global distribution of two fungal pathogens threatening endangered sea turtles

This work was supported by grants of Ministerio de Ciencia e Innovación, Spain (CGL2009-10032, CGL2012-32934). J.M.S.R was supported by PhD fellowship of the CSIC (JAEPre 0901804). The Natural Environment Research Council and the Biotechnology and Biological Sciences Research Council supported P.V.W. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Thanks Machalilla National Park in Ecuador, Pacuare Nature Reserve in Costa Rica, Foundations Natura 2000 in Cape Verde and Equilibrio Azul in Ecuador, Dr. Jesus Muñoz, Dr. Ian Bell, Dr. Juan Patiño for help and technical support during samplingPeer reviewedPublisher PD

Current knowledge, status and future for plant and fungal diversity in Great Britain and the UK Overseas Territories

Societal Impact Statement We rely on plants and fungi for most aspects of our lives. Yet plants and fungi are under threat, and we risk losing species before we know their identity, roles, and potential uses. Knowing names, distributions, and threats are first steps toward effective conservation action. Accessible products like field guides and online resources engage society, harnessing collective support for conservation. Here, we review current knowledge of the plants and fungi of the UK and UK Overseas Territories, highlighting gaps to help direct future research efforts toward conserving these vital elements of biodiversity. Summary This review summarizes current knowledge of the status and threats to the plants and fungi of Great Britain and the UK Overseas Territories (UKOTs). Although the body of knowledge is considerable, the distribution of information varies substantially, and we highlight knowledge gaps. The UK vascular flora is the most well studied and we have a relatively clear picture of its 9,001 native and alien taxa. We have seedbanked 72% of the native and archaeophyte angiosperm taxa and 78% of threatened taxa. Knowledge of the UKOTs flora varies across territories and we report a UKOTs flora comprising 4,093 native and alien taxa. We have conserved 27% of the native flora and 51% of the threatened vascular plants in Kew's Millennium Seed Bank, UK. We need a better understanding of the conservation status of plants in the wild, and progress toward completion or updating national red lists varies. Site‐based protection of key plant assemblages is outlined, and progress in identifying Important Plant Areas analyzed. Knowledge of the non‐vascular flora, especially seaweeds remains patchy, particularly in many UKOTs. The biggest gaps overall are in fungi, particularly non‐lichenized fungi. Considerable investment is needed to fill these knowledge gaps and instigate effective conservation strategies

Diversity of 16S-23S rDNA Internal Transcribed Spacer (ITS) Reveals Phylogenetic Relationships in Burkholderia pseudomallei and Its Near-Neighbors

Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species

Diurnal timing of nonmigratory movement by birds: the importance of foraging spatial scales

Timing of activity can reveal an organism's efforts to optimize foraging either by minimizing energy loss through passive movement or by maximizing energetic gain through foraging. Here, we assess whether signals of either of these strategies are detectable in the timing of activity of daily, local movements by birds. We compare the similarities of timing of movement activity among species using six temporal variables: start of activity relative to sunrise, end of activity relative to sunset, relative speed at midday, number of movement bouts, bout duration and proportion of active daytime hours. We test for the influence of flight mode and foraging habitat on the timing of movement activity across avian guilds. We used 64 570 days of GPS movement data collected between 2002 and 2019 for local (non‐migratory) movements of 991 birds from 49 species, representing 14 orders. Dissimilarity among daily activity patterns was best explained by flight mode. Terrestrial soaring birds began activity later and stopped activity earlier than pelagic soaring or flapping birds. Broad‐scale foraging habitat explained less of the clustering patterns because of divergent timing of active periods of pelagic surface and diving foragers. Among pelagic birds, surface foragers were active throughout all 24 hrs of the day while diving foragers matched their active hours more closely to daylight hours. Pelagic surface foragers also had the greatest daily foraging distances, which was consistent with their daytime activity patterns. This study demonstrates that flight mode and foraging habitat influence temporal patterns of daily movement activity of birds.We thank the Nature Conservancy, the Bailey Wildlife Foundation, the Bluestone Foundation, the Ocean View Foundation, Biodiversity Research Institute, the Maine Outdoor Heritage Fund, the Davis Conservation Foundation and The U.S. Department of Energy (DE‐EE0005362), and the Darwin Initiative (19-026), EDP S.A. ‘Fundação para a Biodiversidade’ and the Portuguese Foundation for Science and Technology (FCT) (DL57/2019/CP 1440/CT 0021), Enterprise St Helena (ESH), Friends of National Zoo Conservation Research Grant Program and Conservation Nation, ConocoPhillips Global Signature Program, Maryland Department of Natural Resources, Cellular Tracking Technologies and Hawk Mountain Sanctuary for providing funding and in-kind support for the GPS data used in our analyses

Isolation of antifungal bacteria from compost and environmental samples

Basal stem rot (BSR) disease caused by Ganoderma boninense infects oil palms and is spreading rapidly in oil palm plantations. This has caused resources to be wasted and resulting economic losses. Much effort has been put into finding a cure for the disease, but no effective method has been reported yet - with many only being able to slow the death of infected oil palms. In recent years, the use of biocontrol agents has been explored, prompting more extensive research to be done. In this project, bacterial strains from environmental and compost samples that inhibited the growth of G. boninense were isolated and examined. They were used to construct a phylogenetic tree based on their 16s rRNA gene distance. Metabolites responsible for the inhibition derived from each of the isolated bacterial strains were studied as well.Bachelor of Engineering (Environmental Engineering

Wearable Technologies in Field Hockey Competitions: A Scoping Review

The aim of this review is to investigate the common wearable devices currently used in field hockey competitions, and to understand the hockey-specific parameters these devices measure. A systematic search was conducted by using three electronic databases and search terms that included field hockey, wearables, accelerometers, inertial sensors, global positioning system (GPS), heart rate monitors, load, performance analysis, player activity profiles, and competitions from the earliest record. The review included 39 studies that used wearable devices during competitions. GPS units were found to be the most common wearable in elite field hockey competitions, followed by heart rate monitors. Wearables in field hockey are mostly used to measure player activity profiles and physiological demands. Inconsistencies in sampling rates and performance bands make comparisons between studies challenging. Nonetheless, this review demonstrated that wearable devices are being used for various applications in field hockey. Researchers, engineers, coaches, and sport scientists can consider using GPS units of higher sampling rates, as well as including additional variables such as skin temperatures and injury associations, to provide a more thorough evaluation of players’ physical and physiological performances. Future work should include goalkeepers and non-elite players who are less studied in the current literature

Isolation of fungal pathogens from eggs of the endangered sea turtle species Chelonia mydas in Ascension Island

Fungal emerging pathogens are one of the main threats for global biodiversity. Sea turtles do not seem to be an exemption, and recent studies on important nesting areas worldwide have shown that two fungal pathogens, i.e. Fusarium falciforme and Fusarium keratoplasticum, are involved in low hatching success in nests of sea turtle species. Although the presence of these pathogens has been detected in Ascension Island, there are no investigations on the distribution of these two pathogens in main nesting beaches in the island. In this study, we analysed 109 eggshells of the species Chelonia mydas from four nesting areas in Ascension Island. We have isolated and identified a total of 46 fungal isolates. A phylogenetic analysis, of the ITS nrDNA region, with a number of reference sequences of the Fusarium solani species complex, showed that 23 of these isolates corresponded to the pathogen F. keratoplasticum. The analyses on isolation frequency, that included other previously obtained isolates, i.e. 11 F. keratoplasticum and one F. falciforme, showed that F. keratoplasticum was the species most frequently isolated in Ascension Island and it was found in all nesting beaches, while F. falciforme was only isolated from Pan Am beach. When compared with other nesting areas worldwide, the abundance of F. keratoplasticum over F. falciforme was higher than any other nesting region tested. These findings are important in order to evaluate the potential threat of this pathogen to nests of the sea turtle population of Ascension Island, and to develop future control strategies.This work was supported by grants of Ministerio de Ciencia e Innovacio´n, Spain (CGL2009-10032, CGL2012-32934), J.M.S.R. was supported by PhD fellowship of the CSIC (JAEPre 0901804). P.v.W. was supported by the University of Aberdeen and a Darwin award to Dr Paul Brickle