Assessing ecological correlates of avian disease prevalence in the Galapagos Islands using GIS and remote sensing

In recognition of the potential consequences of pathogen introduction to the Galapagos Islands, the Saint Louis Zoo and the University of Missouri¿Saint Louis, in cooperation with the Galapagos National Park Service and the Charles Darwin Research Station, implemented an avian disease surveillance program in 2001, with the objective of identifying and monitoring for pathogens that pose risk for native bird populations. The purpose of this thesis is identify environmental factors that might influence the geographic distribution of avian pathogen infection, based on two data sets obtained as a result of these surveillance efforts: 1) seroprevalence data on 10 common poultry pathogens from farm sites within the agricultural zone of Santa Cruz; and 2) prevalence and intensity values of microfilarial infections of endangered flightless cormorants and Gal¿pagos penguins. Putative correlative factors were obtained from various geographic information system (GIS) and remotes sensing data sets, containing information on temperature, precipitation, water vapor, soil moisture, vegetative density and topography. Results of these analyses provide indications of correlation between pathogen infection measures and various ecological factors which may affect disease transmission. These observations may provide the bases for the formulation of specific hypotheses for more rigorous statistical verification. An understanding of the environmental factors influencing poultry pathogen prevalence may be useful in predicting the consequences of pathogen transmission across the poultry/wildlife interface. Insight into the geographic distribution of arthropod-vectored microfilarial infections may allow us to predict the spatial distribution of transmission risk should other arthropod-borne pathogens, such as avian malaria or West Nile Virus, be introduced to this ecosystem

Allometric Regression of Snake Body Length from Head Image Measurements

As in many fields of wildlife research and management, camera devices and photogrammetry have become an integral part of the toolkit for exploring otherwise‐unseen aspects of the biology, behavior, and control of the invasive brown treesnake (Boiga irregularis) on Guam. Because brown treesnakes are cryptic and nocturnal, and nearly all aspects of their ecology are influenced by snake size, methods are needed to estimate snake size from images captured by infrared wildlife cameras. Unfortunately, it is difficult to capture images of an entire snake’s length at a controlled distance from a simple camera setup. Here, I describe the allometric relationships between brown treesnake body length and potential predictors: head measurements, sex, and body condition. Head length (HL) was the most important predictor of body length, alone accounting for 95.9% of the variation in brown treesnake snout‐vent length (SVL). We provide simple regression equations for predicting brown treesnake length from head measurements, an example of how to extract measurements from images, and a convenient lookup table for predicting SVL and 80% prediction intervals from HL alone. Coupled with a simple camera setup that controls subject distance and includes size standards in the image, we can estimate brown treesnake body size from images that include only the head when photographed from above. These methods have been developed to enable ongoing assessments of brown treesnake predation risk following landscape‐scale suppression efforts that could enable the reintroduction of extirpated native wildlife

Microgeographic and ontogenetic variability in the ecology of invasive Brown Treesnakes on Guam, and effects of roads on their landscape-scale movements

2015 Spring.Includes bibliographical references.To view the abstract, please see the full text of the document

Relative acceptance of brodifacoum pellets and soft bait sachets by Polynesian rats (Rattus exulans) on Wake Atoll

Removing invasive rats from island ecosystems using rodenticides has proven conservation benefits and is an important management tool for conserving and restoring island ecosystems. However, rodenticide-based eradications can fail if not all rats consume enough bait to result in lethal toxicosis. A recent post-operational review of a failed attempt to eradicate rats from Wake Atoll suggested that some individuals may not have ingested a lethal dose of rodenticide due to potential dietary and/or sensory preferences developed via regular access to anthropogenic food sources. These food sources may be higher in fats and oils, possessing different sensory properties (e.g., softer, chewier, etc.) than the harder pellet formulation of the rodenticide Brodifacoum 25W Conservation (B-25W) used in the eradication attempt. To test this theory, we captured rats from two areas on Wake Island where they may have regular access to human food sources, as well as an uninhabited part of island where rats presumably have less access to human-based food sources and therefore are less likely to be preconditioned for these food types. We subjected them to a head-to-head two-choice bait selection trial between a “soft” sachet formulation of a brodifacoum-based bait, FINAL Soft Bait with Lumitrack® (FINAL), versus the harder pellet formulation of B-25W. Regardless of which habitat rats were captured in, rats overwhelmingly preferred the pellet formulation. No rats in the head-to-head trail consumed any of the FINAL bait, and 100% of the rats that consumed B-25W died. Of the rats in a separate no-choice trail of just FINAL bait, 5 failed to eat any bait; of the rats that did consume some of the FINAL bait, 80% died. Our results demonstrate that Polynesian rats on Wake Atoll do not prefer this soft formulation of brodifacoum-based rodenticide bait. Our results suggest that baiting strategies in the inhabited regions of the atoll, for a proposed eradication attempt, should continue to focus on utilizing traditional pellet formulations. While these results are unequivocal in our test case, we suggest caution in making inference to other situations where dietary preferences of local rodent populations may differ, and local environmental conditions may make other baiting choices more appropriate and efficacious

Brown Tree Snakes Methods and Approaches for Control

This chapter summarizes the existing and emerging tools and strategies for the control of the invasive brown tree snake (Boiga irregularis, or BTS) on Guam and the prevention of its accidental transport to, and subsequent establishment on, other snake-free Pacific islands. The brown tree snake has long served as an example of the ecological and economic damages that can be wrought by a single generalist vertebrate predator upon introduction to ecosystems that evolved in isolation and without native predators (Fritts and Rodda 1998; Wiles et al. 2003; Rodda and Savidge 2007). Our attempt to summarize the state of the art for control technology development and use is not intended to be an exhaustive survey of all the brown tree snake literature. Rather, it is intended to introduce the reader to the main concepts, methods, and strategic management uses of the tools in an effort to control brown tree snakes on the island landscape and interdict their passage at ports. Significant practical advances for brown tree snake control have been made since the first comprehensive summaries were published by Rodda et al. (1999e), and there is great promise for future refinements and broader implementation for control efforts on an islandwide scale

Invasive Coqui Frogs Are Associated With Differences in Mongoose and Rat Abundances and Diets in Hawaii

With the increasing rate of species being introduced to areas outside of their native ranges, non-natives are likely to interact in ways that influence each other’s populations. The high densities of invasive coqui frogs (Eleutherodactylus coqui) in Hawaii have been hypothesized to increase non-native mongoose (Herpestes auropunctatus) and rat (Rattus spp.) abundances, and in turn increase bird nest depredation rates. We compared the relative abundances of rats and mongooses and artificial bird nest predation rates at 12 sites that had plots with similar habitat invaded and not invaded by coqui frogs across the island of Hawaii. We interpret our results considering mongoose and rat stomach analyses and camera trap data collected to monitor coqui scavengers. We found that coqui presence was associated with 30% greater mongoose abundance and 17% lower Pacific rat (R. exulans) abundance. Based on our diet analyses and scavenging data, both mongooses and rats consume coquis, but mongooses were the most important consumers of coquis, which may have contributed to their increase in coqui plots. We speculate that coquis are competing with rats for invertebrate prey due to reduced Pacific rat abundance and greater amounts of fruit in rat stomachs collected in coqui-invaded compared to uninvaded plots. We did not observe any difference in bird nest predation rates in coqui-invaded and uninvaded plots. Our results suggest that the coqui invasion may increase or decrease non-native mammal populations, and non-native amphibians may serve as both novel prey and competitors to non-native mammals

Ontogenetic and ecological variation in invasion risk of Brown Treesnakes (\u3ci\u3eBoiga irregularis\u3c/i\u3e) on Guam

Size structure within populations of invasive species may have consequences for relative risk at all stages of the invasion process, with implications for management interventions such as interdiction, suppression, and eradication. To assess relative distributions of invasive Brown Treesnakes (Boiga irregularis) among demographic categories of management interest, we undertook the most comprehensive and controlled sampling in \u3e 25 years of research into this ecologically and economically destructive introduced predator. We collected a seasonally-balanced sample of 100 snakes from each of 18 sites, stratified by six habitat types, encompassing the species’ entire extralimital range. Samples indicated significant differences in distributions of female and male snakes among management classes (juvenile, transitional, and mature) by site and habitat. We found substantial heterogeneity in localized population characteristics over relatively small geographic distances, only modest influence of habitat type, higher prevalence of reproductively mature snakes in savanna and urban habitats, and an alarmingly high proportion of snakes that are too small to be effectively targeted by current rodent-baited control tools (mean = 38.2%, range = 19 to 72%). Failure to account for such variability in high risk demographic fractions may hinder successful interventions

An Introduction to the Special Issue: Island Invaders

This is the introductory letter from the associate editors of the special issue on Island Invaders

A Review of Rat Lungworm Infection and Recent Data on Its Definitive Hosts in Hawaii

Rat lungworm (Angiostrongylus cantonensis) is a zoonotic nematode that causes rat lungworm disease (angiostrongyliasis), a potentially debilitating form of meningitis, in humans worldwide. The definitive hosts for rat lungworm are primarily members of the genus Rattus, with gastropods as intermediate hosts. This parasite has emerged as an important public health concern in the United States, especially in Hawaii, where the number of human cases has increased in the last decade. Here we discuss the current knowledge of the rat lungworm, including information on the life cycle and host species, as well as updates on known infection levels. Three species of rats have been unintentionally introduced and become established in Hawaii (Rattus exulans, R. norvegicus, and R. rattus), all of which have been documented as definitive hosts of rat lungworm. Our recent findings indicate that infection levels in rats can vary by species and age. Based on these findings, we also suggest the possibility that R. rattus populations in Hawaii are capable of developing some form of acquired immunity to infection over time, which could have important management implications related to control operations. Information on rat lungworm infection levels and distribution in Hawaii is lacking, especially in rat definitive hosts, and the U.S. Department of Agriculture (USDA) National Wildlife Research Center and the University of Hawaii at Hilo are continuing efforts to help fill these gaps in knowledge

Quantile regression of microgeographic variation in population characteristics of an invasive vertebrate predator

Localized ecological conditions have the potential to induce variation in population characteristics such as size distributions and body conditions. The ability to generalize the influence of ecological characteristics on such population traits may be particularly meaningful when those traits influence prospects for successful management interventions. To characterize variability in invasive Brown Treesnake population attributes within and among habitat types, we conducted systematic and seasonally-balanced surveys, collecting 100 snakes from each of 18 sites: three replicates within each of six major habitat types comprising 95% of Guam\u27s geographic expanse. Our study constitutes one of the most comprehensive and controlled samplings of any published snake study. Quantile regression on snake size and body condition indicated significant ecological heterogeneity, with a general trend of relative consistency of size classes and body conditions within and among scrub and Leucaena forest habitat types and more heterogeneity among ravine forest, savanna, and urban residential sites. Larger and more robust snakes were found within some savanna and urban habitat replicates, likely due to relative availability of larger prey. Compared to more homogeneous samples in the wet season, variability in size distributions and body conditions was greater during the dry season. Although there is evidence of habitat influencing Brown Treesnake populations at localized scales (e.g., the higher prevalence of larger snakes- particularly males-in savanna and urban sites), the level of variability among sites within habitat types indicates little ability to make meaningful predictions about these traits at unsampled locations. Seasonal variability within sites and habitats indicates that localized population characterization should include sampling in both wet and dry seasons. Extreme values at single replicates occasionally influenced overall habitat patterns, while pooling replicates masked variability among sites. A full understanding of population characteristics should include an assessment of variability both at the site and habitat level