Characterization of a Second Bovine Rotavirus Serotype

Bovine rotavirus (BRV) V 1005 was characterized by two-way cross-neutralization tests as a second serotype of BRV. Virions and inner shell particles of 65 nm and 55 nm diameter respectively, and empty capsids of 65 nm and 55 nm diameter were separated by density gradient centrifugation. Three polypeptides of molecular weight 60,000, 36,000 and 28,000 (minor protein) could be identified in the outer shell of virions and in the larger empty capsids. Inner shell particles contained three polypeptides of molecular weight 105,000, 83,000 and 43,000. Both sizes of empty capsids showed two polypeptides of molecular weight 75,000 and 55,000 not found in virions. Pulse-labelling of infected cells revealed eight major and three minor intracellular viral polypeptides. Viral polypeptide synthesis started at about 6 hours p.i. and correlated in time with double-stranded RNA synthesis. As soon as viral polypeptide synthesis was detectable, newly synthesized viral polypeptides were incorporated into intracellular viral particles. Radioactive viral polypeptides appeared without a longer lag period in extracellular viruses from 6 hours p.i. onward

Antibody to serotype 8 rotavirus in Ecuadorian and German children

Only 2 out of 71 German patients infected with rotavirus (3%) and 8 out of 147 German control patients (5%) showed serum antibody to the new serotype 8 rotavirus. Such antibody was detected in the sera of 232 of 870 Ecuadorian children (27%). Twelve Ecuadorian sera showed neutralizing activity only against serotype 8 and not to the other serotypes (1-4) tested, indicating that human serotype 8 rotavirus circulates in South Americ

Reactivity of human serum antibody with lipopolysaccharide O 78 antigen from enterotoxigenic Escherichia coli

Fifteen and five of 20 volunteers challenged with the enterotoxigenic Escherichia coli strain O 78·H11 showed a fourfold titre increase of serum ELISA antibody to the homologous O 78 and the heterologous O 8 lipopolysaccharide antigen, respectively. Sixty-three of 191 sera from 1- to 48-month-old German children showed serum antibody reactive with O 78 antigen, all but two of these O 78-positive sera also showed reactivity with at least one further O antigen. Only 14 of the O 78 reactive sera also showed antibody to heat-labile enterotoxin. In addition, soluble O 8 antigen could inhibit the binding of serum antibody to absorbed O 78 in 68% of the German children. Antibody reactive with O 78 antigen is thus not a reliable serological marker for enterotoxigenic E. coli infection in German childre

Investigation of in the full hadronic final state at CDF with a neural network approach

Abstract In this work we present the results of a neural network (NN) approach to the measurement of the t t production cross-section and top mass in the all-hadronic channel, analyzing data collected at the Collider Detector at Fermilab (CDF) experiment. We have used a hardware implementation of a feed forward neural network, TOTEM , the product of a collaboration of INFN (Istituto Nazionale Fisica Nucleare)—IRST (Istituto per la Ricerca Scientifica e Tecnologica)—University of Trento, Italy. Particular attention has been payed to the evaluation of the systematics specifically related to the NN approach. The results are consistent with those obtained at CDF by conventional data selection techniques

Risk factors for asymptomatic abdominal aortic aneurysm: systematic review and meta-analysis of population-based screening studies.

BACKGROUND: The incidence of and mortality from ruptured abdominal aortic aneurysm (AAA) is increasing. There is uncertainty regarding the indicators which could be used to identify groups at high risk. This issue has been addressed in a systematic review of population-based screening studies. METHODS: MEDLINE and EMBASE were searched, reference lists scanned and manual searches made of eight journals. The search was restricted to four languages (English, German, French and Italian). Population-based studies investigating risk factors associated with screening-detected AAA were included. The following risk factors were considered: sex, smoking, hypertension, diabetes, a history myocardial infarction, and peripheral vascular disease. RESULTS: Fourteen cross-sectional studies met our inclusion criteria. Most studies screened people aged 60 years or older. The prevalence of AAA ranged from 4.1% to 14.2% in men and from 0.35% to 6.2% in women. Male sex showed a strong association with AAA (OR 5.69), whereas smoking (OR 2.41), a history of myocardial infarction (OR 2.28) or peripheral vascular disease (OR 2.50) showed moderate associations. Hypertension was only weakly associated with AAA (OR 1.33) and no association was evident with diabetes (OR 1.02). CONCLUSIONS: The efficacy of screening men aged 60 years or older and women of the same age who smoke or have a history of peripheral or coronary artery disease should be evaluated in randomized controlled trials

An evaluation of GPUs for use in an upgraded ATLAS High Level Trigger

ATLAS is a general purpose particle physics experiment located on the LHC collider at CERN. The ATLAS Trigger system consists of two levels, the first level (L1) implemented in hardware and the High Level Trigger (HLT) implemented in software running on a computing cluster of commodity CPUs. The HLT reduces the trigger rate from the 100 kHz L1 accept rate to 1 kHz for recording, requiring an average per-event processing time of ~300 ms for this task. The HLT selection is based on reconstructing tracks in the Inner Detector and Muon Spectrometer and clusters of energy deposited in the calorimeters (electromagnetic and hadronic). Performing this reconstruction within the available HLT computing cluster resources presents a significant challenge. Future HLT upgrades will result in higher detector occupancies and, consequently, will harden the reconstruction constraints. General purpose Graphics Processor Units (GPGPU) are being evaluated for possible future inclusion in an upgraded HLT computing cluster. We report on a demonstrator that has been developed consisting of GPGPU implementations of the calorimeters clustering and Inner Detector and Muon tracking algorithms integrated within the HLT software framework. We give a brief overview of the algorithm implementation and present preliminary measurements comparing the performance of the GPGPU algorithms with the current CPU versions.Peer Reviewe

Artificial intelligence for renal cancer: From imaging to histology and beyond

Artificial intelligence (AI) has made considerable progress within the last decade and is the subject of contemporary literature. This trend is driven by improved computational abilities and increasing amounts of complex data that allow for new approaches in analysis and interpretation. Renal cell carcinoma (RCC) has a rising incidence since most tumors are now detected at an earlier stage due to improved imaging. This creates considerable challenges as approximately 10%–17% of kidney tumors are designated as benign in histopathological evaluation; however, certain co-morbid populations (the obese and elderly) have an increased peri-interventional risk. AI offers an alternative solution by helping to optimize precision and guidance for diagnostic and therapeutic decisions. The narrative review introduced basic principles and provide a comprehensive overview of current AI techniques for RCC. Currently, AI applications can be found in any aspect of RCC management including diagnostics, perioperative care, pathology, and follow-up. Most commonly applied models include neural networks, random forest, support vector machines, and regression. However, for implementation in daily practice, health care providers need to develop a basic understanding and establish interdisciplinary collaborations in order to standardize datasets, define meaningful endpoints, and unify interpretation

Triggering events with GPUs at ATLAS

The growing complexity of events produced in LHC collisions demands increasing computing power both for the online selection and for the offline reconstruction of events. In recent years there have been significant advances in the performance of Graphics Processing Units (GPUs) both in terms of increased compute power and reduced power consumption that make GPUs extremely attractive for use in a complex particle physics experiments such as ATLAS. A small scale prototype of the full ATLAS High Level Trigger has been implemented that exploits reconstruction algorithms optimized for this new massively parallel paradigm. We discuss the integration procedure followed for this prototype and present the performance achieved and the prospects for the future.Peer Reviewe

Right Research: Modelling Sustainable Research Practices in the Anthropocene

The year 2020 started with a massive bushfire crisis in south eastern Australia, resulting in disruption to many communities, the loss of lives and businesses, an estimated loss of a billion animals and the dirtiest air on the planet in the cities of Sydney, Newcastle and Canberra. With record-high temperatures and a punishing draught lasting several years, the Australian bush was primed to explode into flames. With lightning strikes in national parks, the spontaneous eruptions of bushfire spread from the north coast to the south and inland towards the alpine regions of New South Wales and Victoria. With the very hot year of 2019 affecting other parts of the planet in 2020, the Antarctic Peninsula reached a record 65 degrees Fahrenheit. The chapter that follows reflects the new progressive politics of climate change that emerged in 2019 with large mass demonstrations taking place in Australia and around the world and examines the critical role of universities in the mitigation of climate catastrophe. The following interventions are variably focused on the concept of ‘Living Labs’ where thinking is developed within a problem-solving ethos. The three contributions here offer ways to think about sustainability with specific reference to waste recovery, environmental awareness in urban settings and the contribution that a ‘repair’ mentality can make to a shared and re-cycled economy. With a clear-eyed recommendation that mitigation of climate change starts locally, the premise of the paper is that people can work with what is available as local solutions to specific problems. The impact of this approach can be essential to people who sense the impending catastrophe and who may have experienced the crisis directly through compromises in their health outcomes, the experience of trauma and the loss of property and livelihoods, though through no fault of their own. The links through the Western Sydney University campus, common ground to the authors to both its small bushland outpost and further to the local community it serves, suggest that the boundaries of the campus are permeable – and that Living Labs are both a means and metaphor for thinking about how the campus opens learning and knowledge creation about sustainability for its students, staff and community constituents

Measurement of the ttˉproductioncrosssectionint\bar{t} production cross section in p\bar{p}collisionsat collisions at \sqrt{s}$ = 1.8 TeV

We update the measurement of the top production cross section using the CDF detector at the Fermilab Tevatron. This measurement uses $t\bar{t}$ decays to the final states $e+\nu$+jets and $\mu+\nu$+jets. We search for $b$ quarks from $t$ decays via secondary-vertex identification or the identification of semileptonic decays of the $b$ and cascade $c$ quarks. The background to the $t\bar{t}$ production is determined primarily through a Monte Carlo simulation. However, we calibrate the simulation and evaluate its uncertainty using several independent data samples. For a top mass of 175 $GeV/c^2$, we measure $\sigma_{t\bar{t}}=5.1 \pm 1.5$ pb and $\sigma_{t\bar{t}}=9.2 \pm 4.3$ pb using the secondary vertex and the lepton tagging algorithms, respectively. Finally, we combine these results with those from other $t\bar{t}$ decay channels and obtain $\sigma_{t\bar{t}} = 6.5^{+1.7}_{-1.4}$ pb.Comment: The manuscript consists of 130 pages, 35 figures and 42 tables in RevTex. The manuscript is submitted to Physical Review D. Fixed typo in author lis