Electrophoretic Isoenzyme Patterns In The Yeast Genus Kluyveromyces

Protein electrophoresis was employed to see if it would give some insight into the taxonomic question of delineating species in the yeast genus Kluyveromyces van der Walt emend. van der Walt.;The type strains of the species of the geneus Kluyveromyces sensu van der Walt (1970) were compared for eleven isoenzymes. These enzymes included five oxidoreductases, five hydrolases, and one lyase.;The isoenzyme patterns of of seven enzymes of Kluyveromyces populations from a variety of habitats were compared. The isolates belonged to the Kluyveromyces lactis and K. marxianus deoxyribonucleic acid (DNA) reassociation groups, and to Kluyveromyces dobzhanskii, Kluyveromyces thermotolerans, and Kluyveromyces waltii.;The results of multivariate analysis of the electrophoretic patterns supported the division of the genus into thirteen species, two of which comprised four and five taxa (described by van der Walt, 1970b) respectively. Electrophoretic data were at variance with in vitro mating compatibility patterns but supported DNA-DNA reassociation data.;Finally, interspecific and intraspecific hybrids between K. marxianus and K. thermotolerans and between K. lactis and K. vanudenii, respectively, their parental strains, and their corresponding type cultures were studied by comparison of seven isoenzyme patterns. The parental strains of the interspecific hybrid were very different, and crossing between these two strains did not receive much direct substantiation. The K. marxianus x K. thermotolerans hybrid appeared very similar, electrophoretically, to its K. marxianus parental strain, and had little in common with K. thermotolerans. The loss and gain of bands by the hybrid strain did support Kurtzman\u27s et al. (1983) suggestion that it might be an aneuploid yeast. Even though the parental strains of the intraspecific hybrids had very similar isoenzyme patterns, differences in electrophoretic patterns were observed between parental and hybrid strains, indicating that crossing can occur between K. lactis and K. vanudenii. Certain isoenzymes were found to be better indicators of the hybrid state than other enzymes. The possible relationships between hybrids and populations were explored

The All-Powerful Freedom: Creativity and Resilience in the Context of Friedl Dicker-Brandeis’ Art Teaching Experiment

This paper examines the pedagogical legacy of Friedl Dicker-Brandeis (1898–1944), an avant-garde artist and progressive art teacher of the interwar period. As a collaborative effort, it promotes an interdisciplinary approach to the analysis and interpretation of Friedl’s teaching method, combining art history with education (Reform Pedagogy) and study into resilience (social pedagogy and developmental psychology). While incarcerated in the Terezín ghetto (1942–1944), Friedl organized children’s art classes within which she used an experimental method based on her own schooling experience in Vienna and at the State Bauhaus in Weimar. Offering her students a perfect outlet for processing their traumatic experience by the means of creativity and self-expression, she became a perfect example of what would today be described as a resilience tutor. A group of collages and drawings will be shown and analyzed

The minimal duplex DNA sequence required for site-specific recombination promoted by the FLP protein of yeast in vitro.

The 2-micron plasmid of the yeast Saccharomyces cerevisiae codes for a site-specific recombinase ('FLP') that efficiently catalyses recombination across the plasmid's two 599 bp repeats both in vivo and in vitro. We have used the partially purified FLP protein to define the minimal duplex DNA sequence required for intra- and intermolecular recombination in vitro. Previous DNase footprinting experiments had shown that FLP protected 50 bp of DNA around the recombination site. We made BAL31 deletions and synthetic FLP sites to show that the minimal length of the site that was able to recombine with a wild-type site was 22 bp. The site consists of two 7 bp inverted repeats surrounding an 8 bp core region. We also showed that the deleted sites recombined with themselves and that one of three 13 bp repeated elements within the FLP target sequence was not necessary for efficient recombination in vitro. Mutants lacking this redundant 13 bp element required a lower amount of FLP recombinase to achieve maximal yield of recombination than the wild type site. Finally, we discuss the structure of the FLP site in relation to the proposed function of FLP recombination in copy number amplification of the 2-micron plasmid in vivo

Dem Hoch-Edelgebohrnen und Hochgelahrten Herrn, Herrn Anton Adolph Bergmann, ordentlich-berufenen Rectori der öffentlichen lateinischen Schule zu Stadthagen, Wolten bey den Antritt Seines neuen Lehr-Amts, aus schuldiger Liebe ... hertzlich gratuliren und Glück wünschen, Deroselben gehorsamste Diener und Schüler, J. W. Sidenberg, J. H. Schlüter, und C. W. Wippermann. Den 2. May, 1754.

Glückwunschgedicht auf Anton Adolf Bergmann, Rektor, zum Amtsantritt, 2. Mai 1754Autopsie nach Ex. der ULB Sachsen-AnhaltVorlageform des Erscheinungsvermerks: Stadthagen, Gedruckt von Johann Friedrich Althans, Hoch-Gräflich-Schaumburg-Lippisch-privilegirten Hof-Buchdrucker