334 research outputs found

    Brändäyksellä lentoon! : case: Emilia K.

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    Tämä opinnäytetyö tehtiin toimeksiantona, työprosessin aikana yritystä perustavalle Emilia Karjalaiselle. Toimeksiantajan yritys Emilia K. tarjoaa valmennuspalveluita niin yksityishenkilöille, ryhmille kuin yrityksillekin. Kyseessä oli ensimmäisen yrityksen perustanut nuori naisyrittäjä, jolle brändäys oli näin ollen erittäin ajankohtainen aihe. Opinnäytetyön tavoitteena oli tarkoitus auttaa uutta yrittäjää brändin luomisessa ja selkeyttää, mitä kaikkea brändäys pitää sisällään. Opinnäytetyön teoriaosuus keskittyy brändäyksen teoriaan; mitä se oikeasti tarkoittaa, mistä brändäys on lähtöisin, miksi se on niin tärkeää ja mitä vahvoja brändejä Suomessa ja maailmalla on olemassa. Lisäksi teoriaosuudessa tarkastellaan, mitä brändin rakentaminen käytännössä tarkoittaa ja mitä kaikkea siinä pitää ottaa huomioon. Teoriaosuus on pidetty yksinkertaisena ja mietitty niin, että myös muut aloittelevat mikroyritykset voisivat siitä hyötyä. Teoriaosuuden jälkeen, työssä käsitellään toimeksiantajan kanssa yhdessä työstettyä brändiä ja käydään läpi, mitä kaikkea brändäyksen suhteen tulisi tehdä. Lopputuloksena syntyi aloittelevalle yrittäjälle räätälöity brändi, joka rakentuu kuitenkin jatkuvasti ja kehittyy ajan myötä. Keskeisiksi Emilia K. -brändin kulmakiviksi nousivat aitous, positiivisuus, näkyvyys sosiaalisessa mediassa ja harkiten luodut verkostot.This thesis was commissioned by Emilia Karjalainen, who was at the time of the study working to establish her start-up company. The client company Emilia K. offers coaching services to individuals, groups as well as companies. Branding was thus a very timely topic for the young entrepreneur. The aim of this thesis was to help the new entrepreneur in creating a brand and to clarify what branding encompasses. The theoretical part of the thesis focuses on branding theory: what does it really mean, where does branding originate from, why it is so important and what are the strong brands in Finland and around the world. In addition, the theoretical part examines what brand-building means in practice and the issues that need to be considered regarding branding. The theoretical part has been kept simple and optimized in order for other micro-enterprises to be able to benefit from it also. After the theoretical part, this study focuses on working on the brand together with the client as well as covering all aspects and actions needed to build a brand. The end result was a well thought-out brand, which will grow continuously and evolve over time, intended for the fresh entrepreneur. The cornerstones of the Emilia K. brand are honesty, positivity, visibility in social media and thoughtfully built networks

    A new model for the development of information systems

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    The most commonly used systems specification and design techniques in commercial computing are described and compared; Information Engineering as proposed by James Martin, A Framework for Information Definition-Muitiview proposed by Wood-Harper et al, Real-world Modeling as described by Jackson, Structured Analysis and Design as In Demarco, Yourdon and Constantine and Output-Oriented Structured Requirement Definition proposed by Orr. In addition, system prototyping is discussed, including the role of prototyping in large software development projects and as a tool for the design of human-computer interfaces. Other areas described and discussed include decision support systems (DSS) and knowledge based management support systems. The context is in the design and development approaches for DSS, prototyping for DSS, expert system for DSS and the integration of DSS and information system. The design and development of human-computer interface is also discussed in relation to user Interface complexity and adaptive interfaces. Further, the important issue of user involvement and support within the development process Is discussed. Thus, weaknesses of current approaches to the system development process are identified and a new model for the development of information system is proposed. In proposing the model, data and functional analysis structured method and methodology for decision support systems (DSS) development is presented including guidelines for the development of knowledge based DSS. The new proposed model is put to test in the design, development and implementation of large integrated commercial systems including DSS. Results and discussion on the use of the model is reported with special consideration to the users' and developers' view of the model. Finally the objectives of this research program are examined in relation to what has been achieved during this program of research. The prospect of using the model for the development of information systems are concluded with references to current and future goals

    Novel techniques for microencapsulation of probiotic bacteria

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    Microencapsulation of living cells such as probiotic bacteria can be used for the protection of the cells from harsh conditions such as low pH and mechanical stress in the digestive system. In this thesis we demonstrate various novel strategies to microencapsulate living yeast cells as a model for probiotic bacteria. We prepared and used sporopollenin microcapsules to encapsulate yeast cells by compressing the sporopollenin particles into a pellet which was exposed to an aqueous suspension of yeast cells in the presence of a biocompatible surface active agent. We also demonstrate that the viability of the cells is preserved after the microencapsulation. We fabricated novel shellac-yeast cells composite microcapsules programmed to release the cells upon change of pH in a narrow range. This was achieved by either spray drying or sprays co-precipitating dispersion of yeast cells in aqueous solution of ammonium shellac doped with a pH-sensitive polyelectrolyte. We also demonstrate that yeast cells retain their viability even when treated with aqueous solutions of low pH. In addition, the pH-triggered release of yeast cells from these composite microcapsules and their disintegration rates were investigated. We developed a theoretical model for the kinetics of yeast cells release from the microcapsules triggered by (i) pH change and (ii) the growth of the cells in a culture media. In a separate strategy of microencapsulation of living cells, we used templates of Pickering emulsions stabilised with latex nanoparticles to fabricate colloidosomes loaded with viable probiotics. Depending on the method of transfer, we have shown that magnetic colloidosomes containing pH-sensitive polyelectrolyte loaded with living cells can be prepared using Pickering emulsion templates. In addition, we demonstrate two strategies to strengthen the stability of water-in-oil Pickering emulsion droplets by interlocking the adsorbed latex particle monolayer: by (i) using oppositely charged polyelectrolyte adsorption or (ii) using polyelectrolyte pre-coated yeast cells which act as cross-linkers inside the water-in-oil droplets. Furthermore, we report the fabrication of 3 D multicellular cellosomes of living cells by using water-in-oil emulsion templates as intermediate. We have used two strategies to assemble yeast cells pre-coated with polyelectrolytes in water-in-oil emulsion droplets stabilised with either surfactant or solid particles. The emulsion droplets containing oppositely charged yeast cells linked together by electrostatical interactions were shrunken to compact structures upon addition of dry octanol and subsequently transferred into water to fabricate cellosomes. In summary, this thesis contributes an arsenal of new methods for microencapsulation of living cells for the purpose of their protection and triggered release. The results of this thesis can be used in the formulation of better probiotic products, protection and release of cells implants, tissue engineering and development of live vaccines

    Novel techniques for microencapsulation of probiotic bacteria

    Get PDF
    Microencapsulation of living cells such as probiotic bacteria can be used for the protection of the cells from harsh conditions such as low pH and mechanical stress in the digestive system. In this thesis we demonstrate various novel strategies to microencapsulate living yeast cells as a model for probiotic bacteria. We prepared and used sporopollenin microcapsules to encapsulate yeast cells by compressing the sporopollenin particles into a pellet which was exposed to an aqueous suspension of yeast cells in the presence of a biocompatible surface active agent. We also demonstrate that the viability of the cells is preserved after the microencapsulation.We fabricated novel shellac-yeast cells composite microcapsules programmed to release the cells upon change of pH in a narrow range. This was achieved by either spray drying or sprays co-precipitating dispersion of yeast cells in aqueous solution of ammonium shellac doped with a pH-sensitive polyelectrolyte. We also demonstrate that yeast cells retain their viability even when treated with aqueous solutions of low pH. In addition, the pH-triggered release of yeast cells from these composite microcapsules and their disintegration rates were investigated. We developed a theoretical model for the kinetics of yeast cells release from the microcapsules triggered by (i) pH change and (ii) the growth of the cells in a culture media.In a separate strategy of microencapsulation of living cells, we used templates of Pickering emulsions stabilised with latex nanoparticles to fabricate colloidosomes loaded with viable probiotics. Depending on the method of transfer, we have shown that magnetic colloidosomes containing pH-sensitive polyelectrolyte loaded with living cells can be prepared using Pickering emulsion templates. In addition, we demonstrate two strategies to strengthen the stability of water-in-oil Pickering emulsion droplets by interlocking the adsorbed latex particle monolayer: by (i) using oppositely charged polyelectrolyte adsorption or (ii) using polyelectrolyte pre-coated yeast cells which act as cross-linkers inside the water-in-oil droplets.Furthermore, we report the fabrication of 3 D multicellular cellosomes of living cells by using water-in-oil emulsion templates as intermediate. We have used two strategies to assemble yeast cells pre-coated with polyelectrolytes in water-in-oil emulsion droplets stabilised with either surfactant or solid particles. The emulsion droplets containing oppositely charged yeast cells linked together by electrostatical interactions were shrunken to compact structures upon addition of dry octanol and subsequently transferred into water to fabricate cellosomes.In summary, this thesis contributes an arsenal of new methods for microencapsulation of living cells for the purpose of their protection and triggered release. The results of this thesis can be used in the formulation of better probiotic products, protection and release of cells implants, tissue engineering and development of live vaccines

    Wavelet Transform for Educational Network Data Traffic Analysis

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    Network monitoring and analysis are very important, in order to understand the performance of the networks, the reliability of the networks, the security of the networks, and to identify potential problems. In this chapter, we present our latest work on university network data traffic analysis by using continuous wavelet transform (CWT). With CWT, you can analyse the data and show how the frequency content of the data changes over time. This time dependent frequency varying information, which is lacking in other techniques, such FFT, is very useful for network traffic analysis. A twelve month’s network traffic data, include World Wide Web (WWW) data and Email data were presented in 3D format, by using wavelet transform, we can visualise the hourly, daily, weekly and monthly activities. We will first present the theoretical background, then show the experimental results

    Solving Course Timetable Problem by using Integer Linear Programming (Case Study IE Department of ITS)

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    Making IE department of ITS course timetable by determine the hard and soft constraints then develop an integer programming (ILP) model method to solve this NP-complete problem of Timetabling for solving Hard constraints Assignment problem and to solve the Soft constraints use Penalty Algorithm. Use LINGO software for solving suggested mathematical model to get the final results. Then do numerical analyzes for that results. Finally it achieves the goal for solving the Course Timetable. And get feasible solution of timetable as well as it gets the best required objective what it can get from the case study which is 356 events and it reduces the time of getting one timetable to be just one hour after it was at least 2 weeks

    PENGARUH BRAND PASSION, BRAND COMMITMENT DAN BRAND INTIMACY TERHADAP REPURCHASE INTENTION, POSITIVE WOM DAN PAY PRICE PREMIUM

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    Amid the pressures of industrial competition, emotions from consumers can be an unlimited resource that can create good opportunities. A brand can no longer be seen merely as an identity but as a relationship with consumers. Therefore, manufacturers are expected to wrap these emotions into a brand through the brand love concept. The usage of the concept of brand love is not without reason, but because brand love is the best representation of high-level constructs, one of which includes emotions. Thus this research aims to see how brand passion, brand intimacy and brand commitment build brand love and then have an impact on repurchase intentions, positive WOM and willingness to pay at premium prices on Samsung brands. This study uses primary data obtained through random questionnaire distribution and using target respondents of Samsung brand smartphone users. The results of this study indicate that brand love can be built well by brand passion, brand intimacy and brand commitment which are its dimensions. Brand love also shows that there is a positive relationship with intention to repurchase, positive WOM and willingness to pay at premium price. If it is more detailed then this study can conclude that brand commitment has the biggest contribution in building brand love and the biggest impact of brand love is positive WO
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