1,386 research outputs found
High-throughput on-chip DNA fragmentation
free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging
Effects of vitrectomy and lensectomy on older rhesus macaques: Oxygen distribution, antioxidant status, and aqueous humor dynamics
Genuine tripartite entanglement monotone of dimensional systems
A genuine tripartite entanglement monotone is presented for -dimensional tripartite pure states by introducing a new
entanglement measure for bipartite pure states. As an application, we consider
the genuine tripartite entanglement of the ground state of the exactly solvable
isotropic spin-1/2 chain with three-spin interaction. It is shown that the
singular behavior of the genuine tripartite entanglement exactly signals a
quantum phase transition.Comment: 5 page
Investigation of passive flow control techniques to enhance the stall characteristics of a microlight aircraft
This report investigates the enhancement of aerodynamic stall characteristics of a Skyranger microlight aircraft by the use of passive flow control techniques, namely vortex generators and turbulators. Each flow control device is designed and scaled to application conditions. Force balance measurements and surface oil flow visualisation are carried out on a half-model of the microlight to further investigate the nature of the flow on the aircraft with and without the flow control devices. The results indicate a clear advantage to the use of turbulators compared with vortex generators. Turbulators increased the maximum lift coefficient by 2.8%, delayed the onset of stall by increasing the critical angle by 17.6% and reduced the drag penalty at both lower (pre-stall) and higher angles of attack by 8% compared to vortex generators. With vortex generators applied, the results indicated a delayed stall with an increase in the critical angle by 2% and a reduced drag penalty at higher angles of attack
Differences in Mode of Action of Cochinchinenin A and B on Tetrodotoxin-Resistant Sodium Channels
Purpose: To explore the mechanism of antagonistic interaction between cochinchinenin A and B in modulating tetrodotoxin-resistant (TTR-X) sodium currents.Methods: The time variation of the effects induced by cochinchinenin A and B on the TTX-R sodium currents in dorsal root ganglion (DRG) neurons of rats were observed using whole-cell patch clamp technique. Based on pharmacological fundamental theory, the modes of action of cochinchinenin A and B on TTX-R channels were distinguished.Results: The scatter diagram which reflected the time variation of inhibition effect on TTX-R sodium currents induced by cochinchinenin A fitted well with occupancy theory equation (goodness of fit test, p > 0.05), while that of cochinchinenin B fitted well with rate theory equation (p > 0.05). The rate constants for combination and dissociation between cochinchinenin A and TTX-R sodium channel were (198.7 ± 39.9) x 10-3 and (41.1 ± 6.2) x 10-3 respectively; while corresponding values for combination andassociation between cochinchinenin B and TTX-R sodium channel were (99.9 ± 16.8) x 10-3 and (5.3 ± 0.4) x 10-3 respectively.Conclusion: The main cause of the antagonistic interaction between cochinchinenin A and B may be attributed to the different modes of their action on TTX-R sodium channels.Keywords: Cochinchinenin, Tetrodotoxin-resistant sodium channel, Antagonistic interaction, Occupancy theory, Rate theor
Hairy carbon electrodes studied by cyclic voltammetry and battery discharge testing
Hairy carbon is a new material developed by growing submicron carbon filaments on conventional carbon substrates. Typical substrate materials include carbon black, graphite powder, carbon fibers, and glassy carbon. A catalyst is used to initiate hair growth with carbonaceous gases serving as the carbon source. To study the electrochemical behavior of hairy carbons, cyclic voltammetry (CV) and discharge testing were conducted. In both cases, hairy carbon results surpassed those of the substrate material alone
Graphite Nanoeraser
We present here a method for cleaning intermediate-size (5~50nm)
contamination from highly oriented pyrolytic graphite. Electron beam deposition
causes a continuous increase of carbonaceous material on graphene and graphite
surfaces, which is difficult to remove by conventional techniques. Direct
mechanical wiping using a graphite nanoeraser is observed to drastically reduce
the amount of contamination. After the mechanical removal of contamination, the
graphite surfaces were able to self-retract after shearing, indicating that van
der Waals contact bonding is restored. Since contact bonding provides an
indication of a level of cleanliness normally only attainable in a high-quality
clean-room, we discuss potential applications in preparation of ultraclean
surfaces.Comment: 10 pages, two figure
Andromeda's Parachute: A Bright Quadruply Lensed Quasar at z=2.377
We present Keck Cosmic Web Imager spectroscopy of the four putative images of
the lensed quasar candidate J014709+463037 recently discovered by Berghea et
al. (2017). The data verify the source as a quadruply lensed, broad
absorption-line quasar having z_S = 2.377 +/- 0.007. We detect intervening
absorption in the FeII 2586, 2600, MgII 2796, 2803, and/or CIV 1548, 1550
transitions in eight foreground systems, three of which have redshifts
consistent with the photometric-redshift estimate reported for the lensing
galaxy (z_L ~ 0.57). By virtue of their positions on the sky, the source images
probe these absorbers over transverse physical scales of ~0.3-21 kpc,
permitting assessment of the variation in metal-line equivalent width W_r as a
function of sight-line separation. We measure differences in W_r,2796 of <40%
across all sight-line pairs subtending 7-21 kpc, suggestive of a high degree of
spatial coherence for MgII-absorbing material. W_r,2600 is observed to vary by
>50% over the same scales across the majority of sight-line pairs, while CIV
absorption exhibits a wide range in W_r,1548 differences of ~5-80% within
transverse distances less than ~3 kpc. J014709+463037 is one of only a handful
of z > 2 quadruply lensed systems for which all four source images are very
bright (r = 15.4-17.7 mag) and are easily separated in ground-based seeing
conditions. As such, it is an ideal candidate for higher-resolution
spectroscopy probing the spatial variation in the kinematic structure and
physical state of intervening absorbers.Comment: Submitted to ApJL. 9 pages, 3 figures. Uses aastex61 forma
Transport of skyrmions by surface acoustic waves
Magnetic skyrmions in thin films with perpendicular magnetic anisotropy are promising candidates for magnetic memory and logic devices, making the development of ways to transport skyrmions efficiently in a desired trajectory of significant interest. Here, we investigate the transport of skyrmions by surface acoustic waves (SAWs) via several modalities using micromagnetic simulations. We show skyrmion pinning sites created by standing SAWs at anti-nodes and skyrmion Hall-like motion without pinning driven by traveling SAWs. We also show how orthogonal SAWs formed by combining a longitudinal traveling SAW and a transverse standing SAW can be used for the 2D positioning of skyrmions. Our results also suggest SAWs offer a viable approach to the transport of multiple skyrmions along a multichannel racetrack
Combined meta-analysis of systemic effects of allogeneic stem cell transplantation and systemic sclerosis
Abstract
Background
Chronic graft-versus-host disease (cGVHD) is a major factor of morbidity and mortality for allogeneic stem cell transplantation (aSCT). The skin and internal organ involvement is the most common systemic complication of cGVHD and closely resembles systemic sclerosis (SSc). Circulating lymphocytes characterize the autoimmune nature of both conditions. Therefore we hypothesized that the common clinical manifestation (systemic organ and skin injury) and the common underlying players (lymphocytes) justify the combined meta-analysis of these diseases.
Results
The aSCT and SSc datasets were uploaded from Gene Expression Omnibus (GEO), a public functional genomics data repository. The available microarray studies of peripheral blood mononuclear cells (PBMCs) and isolated lymphocytes were limited to well established microarray platforms (Affymetrix, Agilent, Canvac, and Illumina) and experimental settings with ≥10 patients per group. The resulting pools of data were merged by unique gene identifier and analyzed by the expression genome-wide association studies (eGWAS) coupled with the subtraction of the cGVHD+ and cGVHD− molecular signatures. The eGWAS was applied to 47 and 50 lymphocyte profiles from aSCT and SSc patients, respectively. The identified 35 candidates were represented by 8 known cGVHD genes (including CXCR4, LTBR and PML) and 28 new candidate genes (including SEPX1 and DNJGB1). The further mutual subtraction of cGVHD+ and cGVHD− candidates and pathway analysis identified a list of 25 genes. Seven of these genes belong to the fibroblast development and function pathway, consisting of the well known cGVHD genes CCND1, JUN, and FOS, and the new molecular targets MMP2, FOSB, TNFAIP8, and DUSP1. These genes become primary candidates for a potential link of systemic effects of cGVHD and SSc.
Conclusions
We designed a new approach for meta-analysis by combining data from different diseases using common clinical manifestation as a linker. This allowed us to power up the insufficient standalone meta-analysis of aSCT microarray studies, by adding SSc samples to the data pool. This new method has successfully identified novel molecular targets for systemic effects of both aSCT and SSc. We believe that this approach is generalizable and can be applied to an array of diseases with common clinical manifestations.Peer Reviewe
- …