Optimization of miRNA-seq data preprocessing.

The past two decades of microRNA (miRNA) research has solidified the role of these small non-coding RNAs as key regulators of many biological processes and promising biomarkers for disease. The concurrent development in high-throughput profiling technology has further advanced our understanding of the impact of their dysregulation on a global scale. Currently, next-generation sequencing is the platform of choice for the discovery and quantification of miRNAs. Despite this, there is no clear consensus on how the data should be preprocessed before conducting downstream analyses. Often overlooked, data preprocessing is an essential step in data analysis: the presence of unreliable features and noise can affect the conclusions drawn from downstream analyses. Using a spike-in dilution study, we evaluated the effects of several general-purpose aligners (BWA, Bowtie, Bowtie 2 and Novoalign), and normalization methods (counts-per-million, total count scaling, upper quartile scaling, Trimmed Mean of M, DESeq, linear regression, cyclic loess and quantile) with respect to the final miRNA count data distribution, variance, bias and accuracy of differential expression analysis. We make practical recommendations on the optimal preprocessing methods for the extraction and interpretation of miRNA count data from small RNA-sequencing experiments

The effect of a visual/motion display mismatch in a single axis compensatory tracking task

The frequency response of visual systems is typically unity from 0 to 20 rad/sec, while that of motion systems typically falls off in the vicinity of 6 rad/sec. The question arises as to what effect, if any, such a difference in servomechanism performance has on the simulation. Is pilot performance reduced by the conflict between displays? Would a more realistic simulation occur if the visual servomechanisms were degraded to match the motion servomechanisms? Does the pilot need and use the higher frequency information present in the visual display? The purpose of the experiment is to take a step forward toward answering these questions. Work already in the literature which bears on these questions is outlined. A description is then given of an experiment used to check for the effects of a difference in the performance of the visual and motion servomechanisms (the experiment uses a single-axis, compensatory, roll-tracking task). The results of the experiment are then presented and analyzed

How can Australian businesses win in the information technology stakes?

Australia is one of the highest ranking countries in the world for use of information technology (IT), being third highest for IT investment among OECD (Organisation for Economic Co-operation and Development) countries, yet there are wide differences within Australia in IT use. Some Australian businesses are winning higher productivity gains from the use of IT than others. Why? How can other organisations do better? The lecture will show how above average gains have occurred in some leading firms and in industries such as the Wholesale Trade and Finance and Insurance. Case studies will show how some firms have reaped the benefits of IT investment. Environmental pre-conditions affecting the odds for success, including government policies and infrastructure will also be highlighted. The aim of the lecture is to show how all organisations can adapt the lessons learned in order to improve their own business's position in the competitive race

The Teaching of Procedure Across Common Law Systems

What difference does the teaching of procedure make to legal education, legal scholarship, the legal profession, and civil justice reform? This first of four articles on the teaching of procedure canvasses the landscape of current approaches to the teaching of procedure in four legal systems—the United States, Canada, Australia, and England and Wales—surveying the place of procedure in the law school curriculum and in professional training, the kinds of subjects that “procedure” encompasses, and the various ways in which procedure is learned. Little sustained reflection has been carried out as to the import and impact of this longstanding law school subject. Through a comparative approach, this series of articles explores what difference the approach a particular jurisdiction has chosen to adopt makes for legal education, legal scholarship, the practice of law and the profession, and to civil justice reform in our legal system. En quoi l’enseignement de la procédure civile modifi e-t-il les études juridiques, la recherche juridique, la profession d’avocat et la réforme de la justice civile? Ce premier de quatre articles sur l’enseignement de la procédure dresse le tableau de l’approche actuellement utilisée dans quatre systèmes juridiques – aux États-Unis, au Canada, en Australie, en Angleterre et au Pays de Galles – et se penche sur la place qu’occupe la procédure dans le programme des facultés de droit et dans la formation professionnelle, les matières qui constituent la « procédure » et les diverses façons d’apprendre la procédure. L’importance et l’incidence de cette matière traditionnelle des facultés de droit ont fait jusqu’ici l’objet de fort peu de réfl exion en profondeur. Par le biais d’une approche comparative, cette série d’articles examine en quoi l’approche adoptée dans ces pays modifi e chez nous les études et la recherche juridiques, la pratique du droit, la profession d’avocat et la réforme du système de justice civile

Radiation induced zero-resistance states: a dressed electronic structure effect

Recent results on magnetoresistance in a two dimensional electron gas under crossed magnetic and microwave fields show a new class of oscillations, suggesting a new kind of zero-resistance states. A complete understanding of the effect is still lacking. We consider the problem from the point of view of the electronic structure dressed by photons due to a in plane linearly polarized ac field. The dramatic changes in the dressed electronic structure lead to a interpretation of the new magnetoresistance oscillations as a persistent-current like effect, induced by the radiation field.Comment: 5 pages, 5 figures, revtex4, changes in introduction and added reference

An Evaluation of Organizational and Experience Factors Affecting the Perceived Transfer of U.S. Air Force Basic Combat Skills Training

The United States Air Force is in a state of transformation. Due to ongoing operations in Iraq and Afghanistan, the focus of Basic Military Training is shifting to basic combat skills, or the skills needed to survive and operate in a hostile environment. In this study, basic combat skills training was evaluated using a number of training factors that potentially affect trainees’ perception of training transfer, or their ability to apply the skills they learned in training on the job or in a hostile environment. The analysis used structural equation modeling to evaluate the paths between each of the factors and perceived training transfer. Of the factors analyzed, transfer enhancing activities and perceived utility were found to positively influence perceived training transfer for all training types, while organizational support for training was positive for Law of Armed Conflict training only. Deployment experience was positive for weapons training, but negative for Self-Aid and Buddy Care. Realistic job preview was positively related to training transfer, but was only significant with respect to Self-Aid and Buddy Care training. The results of this research may help enhance basic combat skills training and do so at little or no cost