Immunoglobulin VH Chain Gene Analysis of Peripheral Blood IgM-Producing B Cells in Patients with Kawasaki Disease

PURPOSE: Kawasaki disease is a systemic vasculitis, and its etiology and pathogenesis are still not clear. Our study was undertaken to investigate the characteristics of the activation of B cells in the peripheral blood of Kawasaki disease (KD) patients and evidence of stimulation by superantigens. MATERIALS AND METHODS: Blood samples were obtained from three patients (2 males, 1 female) with KD, who were admitted to our Hospital, Seoul, Korea. The mean age was 1.2 years. Distribution of B cells was studied in the acute and subacute phases of KD patients. From the RNA of B cells, we obtained complementary DNA (cDNA) and performed polymerase chain reaction (PCR). To determine the oligoclonal expansion of immunoglobulin M (IgM) V(H) family, we cloned and sequenced the PCR products from each group and analyzed DNA. RESULTS: In the peripheral blood of acute phase patients, T cells were significantly decreased (p < 0.05), whereas B cells were significantly increased (p < 0.05). When the first PCR was done on the B cell chains, V(H)1 to V(H)6 were all found to be expressed. The number of micro gene clones obtained from 3 patients was 312, and they belonged to V(H)3, V(H)4 and V(H)5 family. M99686 germ line was most frequently used and the next most frequently used, were X92224/J, L21967 and L21964. A similar order was seen in patients. Among the clones, 20 sets of clones showed the same base sequence and this was frequent between V(H)2 and V(H)5. There was one set, which showed almost the same base sequence between different patients, and the homology was 99.5%. Twenty sets of clones that had the same base sequence showed high similarity to the germ line (94 - 100%). Among these, the clones that utilized the M99686 germ line were 4 sets which were most frequent. The 3-dimensional structure of one of these clones showed typical beta, sheet structure of immunoglobulin chains. CONCLUSION: The IgM transcripts expressed by the B cells in the peripheral blood of KD patients in the acute phase of the disease clearly showed an oligoclonal expansion, suggesting that KD is caused not by stimulation of a superantigen, but rather by a conventional antigen.ope

Improvement of the Usability of Online Mentoring Website

The purpose of this study is to improve the usability of the current online mentoring website by deriving what should be improved through assessment and reflecting it to system improvement. The related data such as search log and Think Aloud were collected from user groups (9 users in total), and usability was tested according to the predefined test procedures. The collected data were analyzed, using quantitative methods. In terms of search log, the related items including effectiveness, efficiency, satisfaction and error were quantified according to usability testing standards. Then, descriptive statistics was performed. According to usability comparison before and after system improvement, it has mostly improved such as improved effectiveness (increase by 15 points), better efficiency (reduction by 41 seconds), increase in satisfaction (by 8 points) and decrease in error frequency (decrease by 1.2 times). Usability testing should be viewed as a process, not outcome itself. Therefore, it could be used during system prototype in addition to the current system and useful in system improvement

First Confirmed Report of Powdery Mildew Caused by Erysiphe aquilegiae on Casuarina cunninghamiana in Argentina

Casuarina cunninghamiana Miq. (Fam. Casuarinaceae) is native to Australia and was introduced to Argentina and used as a windbreak, shade tree and for ornamental purposes. In autumn 2009, the potted seedlings in the nursery of La Plata University were found to be infected with a powdery mildew. Symptoms first appeared as thin white colonies, which subsequently developed into an abundant growth on the leaves and young stems. The damage caused by the powdery mildew infection has been observed every year after this initial infection and was again confirmed in April, 2011. There have been a few reports on the presence of powdery mildew on Casuarina spp. (Mantz et al., 2008; Farr and Rossman, 2011). In all the reports, the pathogen was cited to be Oidium sp. Thus, the aim of this work was to identify the causal agent of the powdery mildew observed on casuarina in Argentina

Prevalence of Allergic Diseases among Korean School-age Children: A Nationwide Cross-Sectional Questionnaire Study

The purpose of this study was to investigate the nationwide prevalence of childhood asthma, eczema and other allergic diseases in Korean school-age children (8-11 yr old) and to assess the difference between residential areas. Among 6,279 elementary schools, 427 schools were randomly selected according to residential area (metropolitan, provincial, rural, and industrial area) by the cluster sampling method. Parents of students completed a modified Korean version of a questionnaire formulated by the International Study of Asthma and Allergies in Childhood (ISAAC). Among 50,200 subjects, 31,026 (61.8%) responded, and 30,893 (99.6%) were analyzed. The 12-month prevalence of wheeze, flexural rash, and allergic rhinitis symptoms were 4.8%, 15.3%, and 32.9%, respectively. The prevalence of diagnosis of allergic diseases in boys was higher than that in girls, with the exception of eczema. In both boys and girls, the difference of the prevalence of allergic diseases among industrial, metropolitan and provincial areas was not statistically significant, but the differences between rural area and other areas were significant. Our results support the importance of contextual effect associated with residential area as causative agents of allergic diseases among Korean school-age children

A Case of Extensive Spinal Cysticercosis Involving the Whole Spinal Canal in a Patient with a History of Cerebral Cysticercosis

Although cysticercosis is the most common parasitic disease affecting the central nervous system, spinal cysticercosis is rare. A rare form of spinal cysticercosis involving the whole spinal canal is presented. A 45-year-old Korean male had a history of intracranial cysticercosis and showed progressive paraparesis. Spinal magnetic resonance scan showed multiple cysts compressing the spinal cord from C1 to L1. Three different levels (C1-2, T1-3, and T11-L1) required operation. Histopathological examination confirmed cysticercosis. The patient improved markedly after surgery

A Score-Based Evaluation Model for Rehabilitation of Existing Pumped Storage Hydropower Plant Construction

As the proportion of new and renewable energy increases, power control demands are becoming more frequent due to variability in power generation. As a complementary means against this, the pumped storage hydropower plants (PSHP) are attracting attention as energy storage systems (ESS), but it has high construction costs. Therefore, this study aims to improve the economic feasibility by developing the evaluation model of the existing infrastructure into an upper/lower dam suitable for PSHP. The concept of upper dam capacity is newly defined, and the evaluation index is constructed using normalization. A new evaluation system is presented for five factors: environment, stability, energy, capacity, and economy. Finally, it is tested in the pilot area in Korea. Several candidates, including the PSHP in operation, are found to have been distributed with higher scores. These results will help to satisfy the selection of candidates during the preliminary feasibility study phase, and programming them will enable more accurate and rapid assessment

3-D finite element analysis of the effects of post location and loading location on stress distribution in root canals of the mandibular 1st molar

Objective The purpose of this study was to evaluate, by using finite element analysis, the influence of post location and occlusal loading location on the stress distribution pattern inside the root canals of the mandibular 1st molar. Material and Methods Three different 3-D models of the mandibular 1st molar were established: no post (NP) – a model of endodontic and prosthodontic treatments; mesiobuccal post (MP) – a model of endodontic and prosthodontic treatments with a post in the mesiobuccal canal; and distal post (DP) – a model of endodontic and prosthodontic treatments with a post in the distal canal. A vertical force of 300 N, perpendicular to the occlusal plane, was applied to one of five 1 mm2 areas on the occlusal surface; mesial marginal ridge, distal marginal ridge, mesiobuccal cusp, distobuccal cusp, and central fossa. Finite element analysis was used to calculate the equivalent von Mises stresses on each root canal. Results The DP model showed similar maximum stress values to the NP model, while the MP model showed markedly greater maximum stress values. The post procedure increased stress concentration inside the canals, although this was significantly affected by the site of the force. Conclusions In the mandibular 1st molar, the distal canal is the better place to insert the post than the mesiobuccal canal. However, if insertion into the mesiobuccal canal is unavoidable, there should be consideration on the occlusal contact, making central fossa and distal marginal ridge the main functioning areas