Lack of involvement of known DNA methyltransferases in familial hydatidiform mole implies the involvement of other factors in establishment of imprinting in the human female germline

BACKGROUND: Differential methylation of the two alleles is a hallmark of imprinted genes. Correspondingly, loss of DNA methyltransferase function results in aberrant imprinting and abnormal post-fertilization development. In the mouse, mutations of the oocyte-specific isoform of the DNA methyltransferase Dnmt1 (Dnmt1o) and of the methyltransferase-like Dnmt3L gene result in specific failures of imprint establishment or maintenance, at multiple loci. We have previously shown in humans that an analogous inherited failure to establish imprinting at multiple loci in the female germline underlies a rare phenotype of recurrent hydatidiform mole. RESULTS: We have identified a human homologue of the murine Dnmt1o and assessed its pattern of expression. Human DNMT1o mRNA is detectable in mature oocytes and early fertilized embryos but not in any somatic tissues analysed. The somatic isoform of DNMT1 mRNA, in contrast, is not detectable in human oocytes. In the previously-described family with multi-locus imprinting failure, mutation of DNMT1o and of the other known members of this gene family has been excluded. CONCLUSIONS: Mutation of the known DNMT genes does not underlie familial hydatidiform mole, at least in the family under study. This suggests that trans-acting factors other than the known methyltransferases are required for imprint establishment in humans, a concept that has indirect support from recent biochemical studies of DNMT3L

Soundstudio4D - A VR interface for gestural composition of spatial soundscapes

Presented at the 10th International Conference on Auditory Display (ICAD2004)We describe a software system which enables computergenerated soundscapes to be synthesised, spatialised and edited using a gestural interface. Iterative design and testing of the software interface has taken place in a walk-in, immersive, virtual-reality theatre. Sound spatialisation has been implemented for an 8-speaker array using a Vector Base Amplitude Planning algorithm. The software has been written in Java, JSyn and Java3D with native method calls to sound-cards and sensors

A quantitative evolved gas analysis for extra-terrestrial samples

Evolved gas analysis (EGA) has been successfully applied to the studies of meteorites and Apollo lunar samples. It consists of linear heating of a material with registration of the released volatile compounds, typically using a spectrometric technique. However, so far no quantitative comparison was possible of the amount of gases released during heating of a sample. To address this limitation, we have developed a Quantitative EGA (QEGA) technique using our custom-built Finesse mass spectrometry system. It is based on calibration of the quadrupole mass spectrometer with reference gases (e.g. CO2, CO, H2, O2, N2 or their mixtures with known relative abundances) with known flow rate. The method was tested using simple chemical compounds such as CaCO3, which give well-known amounts of pure gases during their thermal decomposition. We present initial QEGA data on two reference meteorites, Allende and Murchison. Our QEGA work is also informing the design and operation of ProSPA spaceflight instruments being developed to perform analogous experiments in situ on the lunar surface through the European Space Agency's PROSPECT payload on Luna 27

EVALUATING THE EFFECTIVENESS OF THE COURSEWARE DEVELOPMENT PROJECT: PILOT STUDIES

This project involves a comprehensive generalizable and transferable evaluation of the Courseware Development Project (CDP) at Dalhousie University\u27s School of Business Administration. This C$3 million, three-year project is divided into four levels over three time phases. The results of the study of the impact of this unique project are expected to be both relevant and applicable to other universities in Canada and throughout the world. This on-going evaluation of the CDP centers around a systems model where: inputs are divided into drivers and materials; throughputs are the conversion processes on a matrix composed of six parties (faculty, students, staff, administrators, organizational structure and processes, and contributing/participating corporations) as the rows and the four levels of the CDP as the columns; and outputs are divided into manifest and latent variables. Demographic, attitudinal, behavioral, and organizational variables will be used in a time series analysis. Using an action research model over the proposed three-year full study, the researchers will assess which elements Of the project are effective at the end of each year of the evaluation. Based on this information the researchers will keep the effective elements in place for the next year and modify any ineffective elements based on the first year\u27s results and competing theory. This cycle will be repeated after year two. Thus the proposed study will contribute to evaluation methodology as described in this paper by treating simultaneously both a case study and a quasiexperiment of the impact of computers on (business) education. A preliminary description of the effects arising from Level 0, the integration of computers in the business school, and Level 1, courseware development. is given here. The general impact of the project upon faculty, staff, and students is described and preliminary findings are presented

Beagle 2: Mission to Mars — current status

Beagle 2, developed in the UK, was launched on June 2, 2003. It landed on Mars on December 25th, 2003 in Isidis Planitia, a large sedimentary basin. To date, the team is awaiting signals from the Beagle 2 lander. Current status of the mission will be reported

Polysaccharide utilization loci and nutritional specialization in a dominant group of butyrate-producing human colonic Firmicutes

Acknowledgements The Rowett Institute of Nutrition and Health (University of Aberdeen) receives financial support from the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS). POS is a PhD student supported by the Scottish Government (RESAS) and the Science Foundation Ireland, through a centre award to the APC Microbiome Institute, Cork, Ireland. Data Summary The high-quality draft genomes generated in this work were deposited at the European Nucleotide Archive under the following accession numbers: 1. Eubacterium rectale T1-815; CVRQ01000001–CVRQ0100 0090: http://www.ebi.ac.uk/ena/data/view/PRJEB9320 2. Roseburia faecis M72/1; CVRR01000001–CVRR010001 01: http://www.ebi.ac.uk/ena/data/view/PRJEB9321 3. Roseburia inulinivorans L1-83; CVRS01000001–CVRS0 100 0151: http://www.ebi.ac.uk/ena/data/view/PRJEB9322Peer reviewedPublisher PD

Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii

ACKNOWLEDGMENTS We acknowledge support from BBSRC grant no. BB/L009951/1, from the Scottish government Food, Land and People program, and from the Society for Applied Microbiology. E.A.B. is supported by a grant (no. 1349/13) from the Israel Science Foundation (ISF), Jerusalem, Israel, and by a grant from the United States-Israel Binational Science Foundation (BSF). E.A.B. is the incumbent of the Maynard I. and Elaine Wishner Chair of Bio-organic Chemistry. Thanks are due to Fergus Nicol for proteomic analysis and to Auriane Bernard for enzyme assays on stationary-phase cultures. We also thank Julian Parkhill and Keith Turner (Wellcome Trust Sanger Institute, Cambridge, United Kingdom) for making the R. bromii L2-63 genome sequence available for analysis.Peer reviewedPublisher PD