Synergistic effects of ginsenoside Rg3 and cyclophosphamine on tumor growth and angiogenesis in lung cancer

To evaluate the effectiveness of ginsenoside Rg3 alone or in combination with cyclophosphamide (CPA) on tumor growth and angiogenesis in human lung cancer, 54 female athymic mice were transplanted with lung cancer cells (A549) which then were randomly divided into 4 groups: Ginsenoside Rg3 group, CPA group, ginsenoside Rg3 plus CPA group and control group. Ginsenoside Rg3 of 3.0 mg/kg (once/day for 10 days) and CPA of 20.0 mg/kg (once/day for 10 days) were intraperitoneally given to mice for consecutive 10 days. Seven mice selected from each group were sacrificed 18 days later. The survival time of the remaining 7 mice in each group was recorded. The life elongation rate, proliferating cell nuclear antigen labeling index (PCNALI), expression of vascular endothelial cell growth factor (VEGF) and microvessel density (MVD) in the tumor tissues were evaluated. The quality of life of mice with administration of ginsenoside Rg3 alone or ginsenoside Rg3 plus CPA were better with longer survival time, when compared with other groups. The PCNALI, MVD and VEGF expression in mice of the treated groups were significantly lowered when compared with that of the control group. Additionally, the MVD of mice in groups with treatment of ginsenoside Rg3 alone or ginsenoside Rg3 plus CPA were lower than that in the CPA group. Tumor growth and angiogenesis in lung cancer were profoundly inhibited by ginsenoside Rg3 alone or in combination with CPA. The synergistic anticancer effects of ginsenoside Rg3 and CPA improved the survival time in lung cancer.Key words: Ginseng, cyclophosphamide, angiogenesis, lung cancer

Crowdsourcing in China: Exploring the Work Experience of Solo Crowdworkers and Crowdfarm Workers

Recent research highlights the potential of crowdsourcing in China. Yet very few studies explore the workplace context and experiences of Chinese crowdworkers. Those that do, focus mainly on the work experiences of solo crowdworkers but do not deal with issues pertaining to the substantial amount of people working in ‘crowdfarms’. This article addresses this gap as one of its primary concerns. Drawing on a study that involves 48 participants, our research explores, compares and contrasts the work experiences of solo crowdworkers to those of crowdfarm workers. Our findings illustrate that the work experiences and context of the solo workers and crowdfarm workers are substantially different, with regards to their motivations, the ways they engage with crowdsourcing, the tasks they work on, and the crowdsourcing platforms they utilize. Overall, our study contributes to furthering the understandings on the work experiences of crowdworkers in China

Negative pressure irrigation increases vegetable water productivity and nitrogen use efficiency by improving soil water and NO3–-N distributions

peer reviewedNegative pressure irrigation (NPI), which is a new subsurface irrigation technique, promotes vegetable yield, water productivity (WP), and nitrogen use efficiency (NUE). However, it is not clear how NPI improves vegetable growth, especially in terms of water supply characteristics and uniformities of soil water and nitrogen. In this study, a cucumber pot experiment that had 0 kPa (PW1), –5 kPa (PW2), –10 kPa (PW3), –15kPa (PW4), and traditional irrigation (PCK) treatments under nitrogen application (N1) and no application (N0) was conducted to reveal the water supply characteristics of NPI and its effect on vegetable growth. There are two main water supply characteristics: 1) automatically supplying irrigation water based on the consumption of soil water, and 2) keeping soil water content stable during the vegetable growth period. In addition, the relationship between vegetable growth and soil water and NO3–-N distribution uniformities throughout the soil profile was investigated by carrying out two tomato field experiments. The treatments of one tomato experiment were NPI with –5 kPa (F1W) and furrow irrigation (F1CK). We also carried out NPI with –5 kPa (F2W), furrow irrigation (F2CK), and drip irrigation (F2D) in another tomato experiment. The results showed that cumulative water application under N1 was higher than under N0 in the PW1, PW2, and PW3 treatments in the cucumber experiment. Volumetric soil water content under the NPI system was more stable during the vegetative growth period than under traditional irrigation. The NPI system also increased yields under appropriate pressures (–10–0 kPa) compared to the PCK treatment in the cucumber experiment. The NPI in the two tomato experiments reduced fertilizer inputs and irrigation compared to furrow irrigation and drip irrigation. However, the irrigation method had no significant influence on the tomato yield in the two tomato experiments. © 2021 Elsevier B.V

The dominant microorganisms vary with aggregates sizes in promoting soil carbon accumulation under straw application

peer reviewedUnraveling the influence of microbes on C content at aggregate scale is pivotal for promoting soil C accumulation. Previous studies were based mainly on the mutual transformation process between aggregates, the links between the microorganisms in initial aggregates and inner C content and aggregate sizes were still unclear. In this study, the classified aggregates (> 5 mm, 2–5 mm, 1–2 mm, 0.25–1 mm, and 2 mm aggregates. Aggregates of > 5 mm were more capable of improving unstable C accumulation and C derived from straw (Cstraw) than smaller aggregates. Fungi and Gram-negative bacteria (G-) were more important to increasing C accumulation in > 2 mm aggregates, whereas Gram-positive (G+) bacteria dominated in < 2 mm aggregates. The results indicate that the contribution of microorganisms within aggregates to inner C accumulation was associated with aggregate sizes. © 2021 Informa UK Limited, trading as Taylor & Francis Group

Prospective Association of Daily Steps with Cardiovascular Disease: A Harmonized Meta-Analysis

Background: Taking fewer than the widely promoted “10 000 steps per day” has recently been associated with lower risk of all-cause mortality. The relationship of steps and cardiovascular disease (CVD) risk remains poorly described. A meta-analysis examining the dose–response relationship between steps per day and CVD can help inform clinical and public health guidelines. Methods: Eight prospective studies (20 152 adults [ie, ≥18 years of age]) were included with device-measured steps and participants followed for CVD events. Studies quantified steps per day and CVD events were defined as fatal and nonfatal coronary heart disease, stroke, and heart failure. Cox proportional hazards regression analyses were completed using study-specific quartiles and hazard ratios (HR) and 95% CI were meta-analyzed with inverse-variance–weighted random effects models. Results: The mean age of participants was 63.2±12.4 years and 52% were women. The mean follow-up was 6.2 years (123 209 person-years), with a total of 1523 CVD events (12.4 per 1000 participant-years) reported. There was a significant difference in the association of steps per day and CVD between older (ie, ≥60 years of age) and younger adults (ie, <60 years of age). For older adults, the HR for quartile 2 was 0.80 (95% CI, 0.69 to 0.93), 0.62 for quartile 3 (95% CI, 0.52 to 0.74), and 0.51 for quartile 4 (95% CI, 0.41 to 0.63) compared with the lowest quartile. For younger adults, the HR for quartile 2 was 0.79 (95% CI, 0.46 to 1.35), 0.90 for quartile 3 (95% CI, 0.64 to 1.25), and 0.95 for quartile 4 (95% CI, 0.61 to 1.48) compared with the lowest quartile. Restricted cubic splines demonstrated a nonlinear association whereby more steps were associated with decreased risk of CVD among older adults. Conclusions: For older adults, taking more daily steps was associated with a progressively decreased risk of CVD. Monitoring and promoting steps per day is a simple metric for clinician–patient communication and population health to reduce the risk of CVD

Daily steps and all-cause mortality: a meta-analysis of 15 international cohorts

Background Although 10000 steps per day is widely promoted to have health benefits, there is little evidence to support this recommendation. We aimed to determine the association between number of steps per day and stepping rate with all-cause mortality. Methods In this meta-analysis, we identified studies investigating the effect of daily step count on all-cause mortality in adults (aged ≥18 years), via a previously published systematic review and expert knowledge of the field. We asked participating study investigators to process their participant-level data following a standardised protocol. The primary outcome was all-cause mortality collected from death certificates and country registries. We analysed the dose– response association of steps per day and stepping rate with all-cause mortality. We did Cox proportional hazards regression analyses using study-specific quartiles of steps per day and calculated hazard ratios (HRs) with inversevariance weighted random effects models. Findings We identified 15 studies, of which seven were published and eight were unpublished, with study start dates between 1999 and 2018. The total sample included 47 471 adults, among whom there were 3013 deaths (10·1 per 1000 participant-years) over a median follow-up of 7·1 years ([IQR 4·3–9·9]; total sum of follow-up across studies was 297 837 person-years). Quartile median steps per day were 3553 for quartile 1, 5801 for quartile 2, 7842 for quartile 3, and 10 901 for quartile 4. Compared with the lowest quartile, the adjusted HR for all-cause mortality was 0·60 (95% CI 0·51–0·71) for quartile 2, 0·55 (0·49–0·62) for quartile 3, and 0·47 (0·39–0·57) for quartile 4. Restricted cubic splines showed progressively decreasing risk of mortality among adults aged 60 years and older with increasing number of steps per day until 6000–8000 steps per day and among adults younger than 60 years until 8000–10000 steps per day. Adjusting for number of steps per day, comparing quartile 1 with quartile 4, the association between higher stepping rates and mortality was attenuated but remained significant for a peak of 30 min (HR 0·67 [95% CI 0·56–0·83]) and a peak of 60 min (0·67 [0·50–0·90]), but not significant for time (min per day) spent walking at 40 steps per min or faster (1·12 [0·96–1·32]) and 100 steps per min or faster (0·86 [0·58–1·28]). Interpretation Taking more steps per day was associated with a progressively lower risk of all-cause mortality, up to a level that varied by age. The findings from this meta-analysis can be used to inform step guidelines for public health promotion of physical activity

HYDROLOGICAL PROCESSES

Detecting water yield variability due to the small proportional land use and land cover changes in a watershed on the Loess Plateau, Chin