Predictors of Failure from Primary Therapy for Giardiasis in San Diego: A Single Institution Retrospective Review.

This study aimed to determine the presence of giardiasis among HIV patients in San Diego, the rate of failure of metronidazole treatment, and factors associated with treatment failure. We used a 7 year retrospective single-center case series of HIV-infected individuals with giardiasis at University of California San Diego Medical Center. Data were analyzed for the changes in the hematological, biochemical, and immunologic results at pre- and at-diagnosis levels. We also compared the changes at the diagnosis level among patients who were treated successfully and those who experienced treatment failure as defined by retreatment with a second course of antibiotics. In 29 Giardia lamblia-infected HIV patients, following diagnosis of G. lamblia, there was a non-significant decrement in cluster of differentiation 4 (CD4), but a statistically significant increase in the number of white blood cell (WBC). Other indices did not differ between pre- and at-diagnosis levels. Twenty patients (69%) were treated with a single course of metronidazole or tinidazole and seven patients (24.1%) were treated with more than one course of metronidazole. These seven patients had statistically significant higher hemoglobin at the time of diagnosis, but further studies are required to confirm if this is a consistent finding and if this can predict failure from primary therapy

Spanning Boundaries to Identify Archival Literacy Competencies

This paper is a report of a collaborative research project that identified the competencies undergraduate history majors should have related to finding and using archival materials. The boundary-spanning collaboration involved archivists, librarians, and history faculty. Historians have long relied upon archives as essential source material, and recent studies confirmed the continued significance of archives to research in this field. However, there is no detailed listing of the archival research competencies that college history students should attain. Without a clearly defined list upon which history faculty, archivists, and library liaisons to history departments agree, teaching about archives research is difficult and often either inconsistent or non-existent. The purpose of this study was to develop a list of archival research, or “archival literacy,” competencies that could be incorporated into the undergraduate history curriculum by history faculty, librarians, and archivists. The mixed-methods study was conducted in two phases. Phase 1 took place in 2012-13 at one large public research university. It consisted of a review of the literature, compilation of a first draft of archival literacy competencies, review of course syllabi, in-depth interviews with history department faculty, and comments from those faculty and recent graduates on a second draft of archival competencies for history students. The study population in Phase 2 expanded to the history department faculty, archivists, and history liaison librarians (or equivalents) at a stratified random sample of U.S. baccalaureate, masters, and doctoral/research institutions. This involved the viewpoints of different stakeholders at different types of institutions. The report on Phase 1 of this study will be published in American Archivist in November 2014. The data for Phase 2 of this study were collected from February through April 2014. The report on Phase 2 that includes the final list of archival literacy competencies has been submitted to a journal

Archival Literacy Competencies for Undergraduate History Majors

Undergraduate history majors need to know how to conduct archival research. This paper describes the second phase of a project to identify “archival literacy” competencies. Faculty, archivists, and librarians from baccalaureate, masters, and doctoral/research institutions commented on a draft list. This resulted in competencies in six major categories: accurately conceive of primary sources; locate primary sources; use a research question, evidence, and argumentation to advance a thesis; obtain guidance from archivists; demonstrate acculturation to archives; and follow publication protocols. Collaborations of archivists, faculty, and librarians can integrate the competencies throughout undergraduate history curricula in their institutions

Computational NMR investigation of mixed-metal (Al,Sc)-MIL-53 and its phase transitions

Funding: The authors would like to thank the ERC (Advanced Grant 787073 ADOR) and the Allan Handsel Postgraduate Research Scholarship for Chemistry for studentship funding for ZHD and EALB, respectively. We also acknowledge support from the Collaborative Computational Project on NMR Crystallography (CCP-NC) funded by EPSRC (EP/T026642/1) and from the UK Materials and Molecular Modelling Hub (Young), which is partially funded by EPSRC (EP/T022213/1, EP/W032260/1 and EP/P020194/1) for which access was obtained via the UKCP consortium and funded by EPSRC (EP/P022561/1).Compositionally complex metal-organic frameworks (MOFs) have properties that depend on local structure that is often difficult to characterise. In this paper a density functional theory (DFT) computational study of mixed-metal (Al,Sc)-MIL-53, a flexible MOF with several different forms, was used to calculate the relative energetics of these forms and to predict NMR parameters that can be used to evaluate whether solid-state NMR spectroscopy can be used to differentiate, identify and characterise the forms adopted by mixed-metal MOFs of different composition. The NMR parameters can also be correlated with structural features in the different forms, giving fundamental insight into the nature and origin of the interactions that affect nuclear spins. Given the complexity of advanced NMR experiments required, and the potential need for expensive and difficult isotopic enrichment, the computational work is invaluable in predicting which experiments and approaches are likely to give the most information on the disorder, local structure and pore forms of these mixed-metal MOFs.Publisher PDFPeer reviewe

Adenovirus serotype 5 E1A sensitizes tumor cells to NKG2D-dependent NK cell lysis and tumor rejection

The expression of the Adenovirus serotype 5 (Ad5) E1A oncogene sensitizes tumor cells to natural killer (NK) cell–mediated killing and tumor rejection in vivo. These effects are dependent on the ability of E1A to bind the transcriptional coadaptor protein p300. To test the hypothesis that E1A up-regulates ligands recognized by the NKG2D-activating receptor, we stably transfected the highly tumorigenic mouse fibrosarcoma cell line MCA-205 with Ad5-E1A or a mutant form of E1A that does not interact with p300 (E1A-Δp300). Ad5-E1A, but not E1A-Δp300, up-regulated the expression of the NKG2D ligand retinoic acid early inducible (RAE)-1, but not murine ULBP-like transcript 1, another NKG2D ligand, in four independently derived MCA-205 transfectants. The up-regulation of RAE-1 by E1A targeted MCA-205 tumor cells to lysis by NK cells, resulting in NKG2D-dependent tumor rejection in vivo. Moreover, the up-regulation of NKG2D ligands by E1A was not limited to mouse tumor cells, as E1A also increased the expression of NKG2D ligands on primary baby mouse kidney cells, human MB435S breast cancer cells, and human H4 fibrosarcoma cells

The Ursinus Weekly, October 23, 1961

Lettinger to lead freshmen; Kelly elected vice-president • Sororities accept sixty new sisters • Two rediscovered works highlight woodwind five\u27s Bomberger performance • Minnich, Weiss win state Young Republican offices • Lawrence calls collegians for press conference: Weekly editor represents Ursinus at Capitol confab • Ursinus to hear Maine\u27s Senator Smith; Receives honorary degree on Founders Day • Efficiency, devotion to duty mark Margaret Chase Smith • Pradervand, Zabarah come to Ursinus from Switzerland, Yemen for studies • President lauds Parents Day; Excellent preparations cited • Editorial: Credit where credit is due • Ursinus in the past • Education by travel • Letters to the editor • 30 yard run breaks Bears backs following 12-6 victory over Wilkes • Hockey lassies remain unbeaten; Defeat Swarthmore, tie Temple girls • Bakermen boot first foes; Lose third tilt to Hens, 2-0 • Hakanson\u27s heft plugs defensive hole; Massachusetts man UC\u27s middle guard • Dean Kellow talks on medical school • Dr. Armstrong organizing second European Tour • German Club members get first-hand Berlin accounthttps://digitalcommons.ursinus.edu/weekly/1301/thumbnail.jp

The Ursinus Weekly, October 21, 1977

Ursinus news in brief: Espada speaks at conference; Ursinus Homecoming; Buy a slave! • Craft elected as officer • New dining style proves popular • Board to vote on calendar • Students experience new dining style • Student employment remains stable • Comment: Student involvement; On cocktail grading • Letters to the editor • 1.2 million raised: Advance Ursinus first year • The Power behind Ursinus mediocrity • Homecoming candidates • Ursinus and the first digital computer • Bears finally come out of the woods • Varsity & JV hockey • C.C. 7-1! • 3 & 4: All wins • Volleyball: Dig it! • Bears winhttps://digitalcommons.ursinus.edu/weekly/1073/thumbnail.jp

The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA

Numerous RNA binding proteins are deposited onto an mRNA transcript to modulate post-transcriptional processing events ensuring proper mRNA maturation. Defining the interplay between RNA binding proteins that couple mRNA biogenesis events is crucial for understanding how gene expression is regulated. To explore how RNA binding proteins control mRNA processing, we investigated a role for the evolutionarily conserved polyadenosine RNA binding protein, Nab2, in mRNA maturation within the nucleus. This work reveals that nab2 mutant cells accumulate intron-containing pre-mRNA in vivo. We extend this analysis to identify genetic interactions between mutant alleles of nab2 and genes encoding the splicing factor, MUD2, and the RNA exosome, RRP6, with in vivo consequences of altered pre-mRNA splicing and poly(A) tail length control. As further evidence linking Nab2 proteins to splicing, an unbiased proteomic analysis of vertebrate Nab2, ZC3H14, identifies physical interactions with numerous components of the spliceosome. We validated the interaction between ZC3H14 and U2AF2/U2AF^(65). Taking all the findings into consideration, we present a model where Nab2/ZC3H14 interacts with spliceosome components to allow proper coupling of splicing with subsequent mRNA processing steps contributing to a kinetic proofreading step that allows properly processed mRNA to exit the nucleus and escape Rrp6-dependent degradation

Primary Ciliary Dyskinesia: First Health-related Quality of Life Measures for Pediatric Patients

Rationale: Primary ciliary dyskinesia (PCD) is a rare disease. There are no available data on disease-specific pediatric patient–reported outcomes

A Prospective Study of the Association of Metacognitive Beliefs and Processes with Persistent Emotional Distress After Diagnosis of Cancer

Two hundred and six patients, diagnosed with primary breast or prostate cancer completed self-report questionnaires on two occasions: before treatment (T1) and 12 months later (T2). The questionnaires included: the Hospital Anxiety and Depression Scale; Impact of Events Scale; the Metacognitions Questionnaire-30 (MCQ-30) and the Illness Perceptions Questionnaire-revised. A series of regression analyses indicated that metacognitive beliefs at T1 predicted between 14 and 19 % of the variance in symptoms of anxiety, depression and trauma at T2 after controlling for age and gender. For all three outcomes, the MCQ-30 subscale ‘negative beliefs about worry’ made the largest individual contribution with ‘cognitive confidence’ also contributing in each case. For anxiety, a third metacognitive variable, ‘positive beliefs about worry’ also predicted variance in T2 symptoms. In addition, hierarchical analyses indicated that metacognitive beliefs explained a small but significant amount of variance in T2 anxiety (2 %) and T2 depression (4 %) over and above that explained by demographic variables, T1 symptoms and T1 illness perceptions. The findings suggest that modifying metacognitive beliefs and processes has the potential to alleviate distress associated with cancer