Increased homocysteine levels impair reference memory and reducecortical levels of acetylcholine in a mouse model of vascular cognitive impairment

Folates are B-vitamins that are vital for normal brain function. Deficiencies in folates either genetic(methylenetetrahydrofolate reductase, MTHFR) or dietary intake of folic acid result in elevated levelsof homocysteine. Clinical studies have shown that elevated levels of homocysteine (Hcy) may be associ-ated with the development of dementia, however this link remains unclear. The purpose of this study wasto evaluate the impact of increased Hcy levels on a mouse model of vascular cognitive impairment (VCI)produced by chronic hypoperfusion. Male and female Mthfr+/+and Mthfr+/−mice were placed on eithercontrol (CD) or folic acid deficient (FADD) diets after which all animals underwent microcoil implantationaround each common carotid artery or a sham procedure. Post-operatively animals were tested on theMorris water maze (MWM), y-maze, and rotarod. Animals had no motor impairments on the rotarod,y-maze, and could learn the location of the platform on the MWM. However, on day 8 of testing of MWMtesting during the probe trial, Mthfr+/−FADD microcoil mice spent significantly less time in the targetquadrant when compared to Mthfr+/−CD sham mice, suggesting impaired reference memory. All FADDmice had elevated levels of plasma homocysteine. MRI analysis revealed arterial remodeling was present in Mthfr+/− microcoil mice not Mthfr+/+ mice. Acetylcholine and related metabolites were reduced in cortical tissue because of microcoil implantation and elevated levels of homocysteine. Deficiencies in folate metabolism resulting in increased Hcy levels yield a metabolic profile that increases susceptibility to neurodegeneration in a mouse model of VCI

Factors Associated with Negative Direct Sputum Examination in Asian and African HIV-Infected Patients with Tuberculosis (ANRS 1260)

OBJECTIVE: To identify factors associated with negative direct sputum examination among African and Cambodian patients co-infected by Mycobacterium tuberculosis and HIV. DESIGN: Prospective multicenter study (ANRS1260) conducted in Cambodia, Senegal and Central African Republic. METHODS: Univariate and multivariate analyses (logistic regression) were used to identify clinical and radiological features associated with negative direct sputum examination in HIV-infected patients with positive M. tuberculosis culture on Lowenstein-Jensen medium. RESULTS: Between September 2002 and December 2005, 175 co-infected patients were hospitalized with at least one respiratory symptom and pulmonary radiographic anomaly. Acid-fast bacillus (AFB) examination was positive in sputum samples from 110 subjects (63%) and negative in 65 patients (37%). Most patients were at an advanced stage of HIV disease (92% at stage III or IV of the WHO classification) with a median CD4 cell count of 36/mm³. In this context, we found that sputum AFB negativity was more frequent in co-infected subjects with associated respiratory tract infections (OR = 2.8 [95%CI:1.1-7.0]), dyspnea (OR = 2.5 [95%CI:1.1-5.6]), and localized interstitial opacities (OR = 3.1 [95%CI:1.3-7.6]), but was less frequent with CD4 ≤ 50/mm³ (OR = 0.4 [95%CI:0.2-0.90), adenopathies (OR = 0.4 [95%CI:0.2-0.93]) and cavitation (OR = 0.1 [95%CI:0.03-0.6]). CONCLUSIONS: One novel finding of this study is the association between concomitant respiratory tract infection and negative sputum AFB, particularly in Cambodia. This finding suggests that repeating AFB testing in AFB-negative patients should be conducted when broad spectrum antibiotic treatment does not lead to complete recovery from respiratory symptoms. In HIV-infected patients with a CD4 cell count below 50/mm3 without an identified cause of pneumonia, systematic AFB direct sputum examination is justified because of atypical clinical features (without cavitation) and high pulmonary mycobacterial burden

Phase-wise enhanced voltage support from electric vehicles in a Danish low-voltage distribution grid

High deployment of electric vehicles (EVs) imposes great challenges for the distribution grids, especially in unbalanced systems with notable voltage variations which detrimentally affect security of supply. On the other hand, with development of Vehicle-to-Grid technology, EVs may be able to provide numerous services for grid support, e.g., voltage control. Implemented electronic equipment will allow them to exchange reactive power for autonomous voltage support without communicating with the distribution system operator or influencing the available active power for primary transportation function. This paper proposes a voltage dependent EV reactive power control and quantifies its impact on a real Danish low-voltage grid. The observed network is a heavily unbalanced three-phase four-wire grid modeled in Matlab SimPowerSystems based on real hourly measurement data. Simulations are performed in order to evaluate phase-to-neutral voltage support benefits as well as to address neutral-to-ground values, active power losses and the unbalances at the same time. The analysis shows that reactive power supportboth raises minimum phase-to-neutral voltage magnitudes and improves voltage dispersion while the energy losses are not notably increased. Further on, since the control is voltage dependent, provided reactive power is unequal among the phases leading to greater support on heavily loaded phases and decreased unbalances caused by residential consumption. Hence, implementation of such a phase-wise enhanced voltage support could defer the need for grid reinforcement in case of large EV penetration rates, especially in highly unbalanced networks

Measurement of the inclusive charmless semileptonic branching ratio of B mesons and determination of vertical bar V-ub vertical bar

We report a measurement of the inclusive charmless semileptonic branching fraction of B mesons in a sample of 89x10(6) B(b) over bar events recorded with the BABAR detector at the Y(4S) resonance. Events are selected by fully reconstructing the decay of one B meson and identifying a charged lepton from the decay of the other B meson. The number of signal events is extracted from the mass distribution of the hadronic system accompanying the lepton and is used to determine the ratio of branching fractions B((B) over bar --> X(u)l (v) over bar)/B((B) over bar --> Xl (v) over bar) = [2.06 +/- 0.25(stat) +/- 0.23(syst) +/- 0.36(theo)] x 10(-2). Using the measured branching fraction for inclusive semileptonic B decays, we find B((B) over bar --> X(u)l (V) over bar) = [2.24+/-0.27(stat) +/- 0.26(syst) +/- 0.39(theo)] x 10(-3) and derive the Cabibbo-Kobayashi-Maskawa matrix element parallel toV(ub)parallel to = [4.62 +/- 0.28(stat) +/- 0.27(syst) +/- 0.48(theo)] x 10(-3)

Final results of an experimental study of a large-area planar drift chamber filled with a He-based mixture

This paper reports the final results of an accurate and complete experimental study of the performance of a large-area (1150 × 566 mm2) planar drift chamber filled with a HeiC4H10 (70−30%) mixture. Sixteen chambers of this type will constitute the elements of the central part of the tracking device of FINUDA, one of the two spectrometers approved for running at DAΦNE. The measured spatial resolution across the full drift cell ranges from 80 to 120 μm for particles at normal incidence angle, and the measured spatial resolution along the sense wire, as measured by the charge division method, was better than 1% of the wire length. Efficiency, space-time relationship, long term stability and Lorentz angle were measured as well. Particular care was devoted to the study of the spatial resolution as a function of the incidence angle. The conclusion of this study is that the chamber performances are the same or better than those obtained with Ar-based mixtures