Rapid astrocyte and microglial activation following pilocarpine-induced seizures in rats.

Astrocyte and microglial activation occurs following seizures and plays a role in epileptogenesis. However, the precise temporal and spatial response to seizures has not been fully examined. The pilocarpine model of temporal lobe epilepsy was selected to examine glial changes following seizures because morphological changes in the hippocampus closely mimic the human condition. Astrocytic and microglial changes in the hippocampus were examined during the first 5 days after pilocarpine-induced seizures in rats by analyzing GFAP, Iba1 and S100B-immunolabeling in CA1, CA3, and the hilus. Also, 3-dimensional reconstructions of microglial cells from the hilus and granule cell layer were analyzed. Lastly, astrocyte hypertrophy was examined in the hilus using electron microscopy. At 1 day after seizures and continuing throughout the 5 days examined, hypertrophied Iba1-labeled microglial cells and glial fibrillary acidic protein (GFAP)-labeled astrocytes were observed. At 1 and 2 days after seizures, significantly greater Iba1 immunolabeling was observed in CA1, CA3, and the hilus. In addition, both the area of Iba1 labeled processes and the number of their endings were increased in the hilus beginning at 1 day after seizures. S100B-immunolabeling was significantly elevated in CA3 at 1 day, in CA3 and CA1 at 2 days, and in all three hippocampal regions at 3 days after seizures. Electron microscopy confirmed astrocytic hypertrophy and demonstrated astrocytic cell bodies in the location where glial endfeet normally appear on capillaries. The differential response patterns of astrocytes and microglial cells following pilocarpine-induced seizures may signify their detrimental role in neuroinflammation after seizures

Limiting opportunities for cheating stabilizes virulence in insect parasitic nematodes

Cooperative secretion of virulence factors by pathogens can lead to social conflict when cheating mutants exploit collective secretion, but do not contribute to it. If cheats outcompete cooperators within hosts, this can cause loss of virulence. Insect parasitic nematodes are important biocontrol tools that secrete a range of significant virulence factors. Critically, effective nematodes are hard to maintain without live passage, which can lead to virulence attenuation. Using experimental evolution, we tested whether social cheating might explain unstable virulence in the nematode Heterorhabditis floridensis by manipulating relatedness via multiplicity of infection (MOI), and the scale of competition. Passage at high MOI, which should reduce relatedness, led to loss of fitness: virulence and reproductive rate declined together and all eight independent lines suffered premature extinction. As theory predicts, relatedness treatments had more impact under stronger global competition. In contrast, low MOI passage led to more stable virulence and increased reproduction. Moreover, low MOI lineages showed a trade-off between virulence and reproduction, particularly for lines under stronger between-host competition. Overall, this study indicates that evolution of virulence theory is valuable for the culture of biocontrol agents: effective nematodes can be improved and maintained if passage methods mitigate possible social conflicts

ARtonomous: Introducing Middle School Students to Reinforcement Learning Through Virtual Robotics

Typical educational robotics approaches rely on imperative programming for robot navigation. However, with the increasing presence of AI in everyday life, these approaches miss an opportunity to introduce machine learning (ML) techniques grounded in an authentic and engaging learning context. Furthermore, the needs for costly specialized equipment and ample physical space are barriers that limit access to robotics experiences for all learners. We propose ARtonomous, a relatively low-cost, virtual alternative to physical, programming-only robotics kits. With ARtonomous, students employ reinforcement learning (RL) alongside code to train and customize virtual autonomous robotic vehicles. Through a study evaluating ARtonomous, we found that middle-school students developed an understanding of RL, reported high levels of engagement, and demonstrated curiosity for learning more about ML. This research demonstrates the feasibility of an approach like ARtonomous for 1) eliminating barriers to robotics education and 2) promoting student learning and interest in RL and ML.Comment: In Proceedings of Interaction Design and Children (IDC '22

Disk heating agents across the Hubble sequence

We measure the shape of the velocity ellipsoid in two late-type spiral galaxies (Hubble types Sc and Scd) and combine these results with our previous analyses of six early-type spirals (Sa to Sbc) to probe the relation between galaxy morphology and the ratio of the vertical and radial dispersions. We confirm at much higher significance (99.9 percent) our prior detection of a tight correlation between these quantities. We explore the trends of the magnitude and shape of the velocity ellipsoid axes with galaxy properties (colour, gas surface mass density, and spiral arm structure). The observed relationships allow for an observational identification of the radial and vertical disk heating agents in external disk galaxies.Comment: 11 pages, 8 figures, 4 tables. Accepted for publication in MNRA

PHIBSS: molecular gas content and scaling relations in z~1-3 normal star forming galaxies

We present PHIBSS, the IRAM Plateau de Bure high-z blue sequence CO 3-2 survey of the molecular gas properties in normal star forming galaxies (SFGs) near the cosmic star formation peak. PHIBSS provides 52 CO detections in two redshift slices at z~1.2 and 2.2, with log(M*(M_solar))>10.4 and log(SFR(M_solar/yr))>1.5. Including a correction for the incomplete coverage of the M*-SFR plane, we infer average gas fractions of ~0.33 at z~1.2 and ~0.47 at z~2.2. Gas fractions drop with stellar mass, in agreement with cosmological simulations including strong star formation feedback. Most of the z~1-3 SFGs are rotationally supported turbulent disks. The sizes of CO and UV/optical emission are comparable. The molecular gas - star formation relation for the z=1-3 SFGs is near-linear, with a ~0.7 Gyrs gas depletion timescale; changes in depletion time are only a secondary effect. Since this timescale is much less than the Hubble time in all SFGs between z~0 and 2, fresh gas must be supplied with a fairly high duty cycle over several billion years. At given z and M*, gas fractions correlate strongly with the specific star formation rate. The variation of specific star formation rate between z~0 and 3 is mainly controlled by the fraction of baryonic mass that resides in cold gas.Comment: Submitted to the Astrophysical Journal; 14 figure

The Ursinus Weekly, April 7, 1977

Ursinus news in brief: New CCC organized; Class elections to be held; Volunteers needed at Norristown; Exec. Comm. meets with Pres.; Late examination fee cancelled; Night school requirement changed • WCC meets food service rep. • New asst. to Harris chosen • Cub and Key inducts new members • Comment: A fond farewell to a close friend; Initial optimism of a new editor • Weekly special: FBI warns of more terrorist attacks • Letters to the editor • Movie attack: Bomb renamed • A low-scale of relief • Alumni assoc. gives gift • Dog Day afternoon • Forum review: Horrors recalled • Meisters prepare tour • Women\u27s basketball reaches nationals • USGA survey • Senior dance • 1977 baseball: title bound? • Bears begin seasonhttps://digitalcommons.ursinus.edu/weekly/1069/thumbnail.jp