3 research outputs found
In vivo identification of novel TGIF2LX target genes in colorectal adenocarcinoma using the cDNA-AFLP method
Background and study aims: Homeobox-containing genes are composed of a group of regulatory genes
encoding transcription factors involved in the control of developmental processes. The homeodomain
proteins could activate or repress the expression of downstream target genes. This study was conducted
to in vivo identify the potential target gene(s) of TGIF2LX in colorectal adenocarcinoma.
Methods: A human colorectal adenocarcinoma cell line, SW48, was transfected with the recombinant
pEGFPN1-TGIF2LX. The cells were injected subcutaneously into the flank of the three groups of
6-week-old female athymic C56BL/6 nude mice (n = 6 per group). The transcript profiles in the developed
tumours were investigated using the cDNA amplified fragment length polymorphism (cDNA-AFLP)
technique.
Results: The real-time RT-PCR and DNA sequencing data for the identified genes indicated that the
N-terminal domain-interacting receptor 1 (Nir1) gene was suppressed whereas Nir2 and fragile histidine
triad (FHIT) genes were upregulated followed by the overexpression of TGIF2LX gene.
Conclusion: Downregulation of Nir1 and upregulation of Nir2 and FHIT genes due to the overexpression
of TGIF2LX suggests that the gene plays an important role as a suppressor in colorectal adenocarcinoma.
� 2018 Pan-Arab Association of Gastroenterology. Published by Elsevier B.V. All rights reserved
Transforming Growth Factor Beta-Induced Factor 2-Linked X (TGIF2LX) Regulates Two Morphogenesis Genes, Nir1 and Nir2 in Human Colorectal
Abstract- A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor
gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional
evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the
human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX
construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic
analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of
TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells
were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results
revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively.
The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the
complex molecular pathways underlying colorectal cancer development