Abstract- A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor
gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional
evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the
human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX
construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic
analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of
TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells
were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results
revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively.
The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the
complex molecular pathways underlying colorectal cancer development