The interstellar oxygen-K absorption edge as observed by XMM-Newton

High resolution X-ray spectra of the Reflection Grating Spectrometer (RGS) on board the XMM satellite are used to resolve the oxygen K absorption edge. By combining spectra of low and high extinction sources, the observed absorption edge can be split in the true interstellar (ISM) extinction and the instrumental absorption. The detailed ISM edge structure closely follows the edge structure of neutral oxygen as derived by theoretical R-matrix calculations. However, the position of the theoretical edge requires a wavelength shift. In addition the detailed instrumental RGS absorption edge structure is presented. All results are verified by comparing to a subset of Chandra LETG-HRC observations.Comment: LaTeX2e A&A style, 10 pages, 12 postscript figures, accepted for publication in Astronomy and Astrophysic

On the reliability of the theoretical internal conversion coefficients

Possible sources of uncertainties in the calculations of the internal conversion coefficients are studied. The uncertainties induced by them are estimated.Comment: 16 pages (including 3 figures inserted by 'epsfig' macro

Functional Relationship between Protein Disulfide Isomerase Family Members during the Oxidative Folding of Human Secretory Proteins

We systematically depleted PDI family members and show that whereas ERp72 and P5 contributed minimally to oxidative protein folding, PDI and ERp57 were the predominant catalysts. Depletion of PDI or ERp57 alone modestly delayed folding, but depletion of both led to generalized protein misfolding and degradation

Discovery of X-rays from Venus with Chandra

On January 10 and 13, 2001, Venus was observed for the first time with an X-ray astronomy satellite. The observation, performed with the ACIS-I and LETG/ACIS-S instruments on Chandra, yielded data of high spatial, spectral, and temporal resolution. Venus is clearly detected as a half-lit crescent, with considerable brightening on the sunward limb. The morphology agrees well with that expected from fluorescent scattering of solar X-rays in the planetary atmosphere. The radiation is observed at discrete energies, mainly at the O-K_alpha energy of 0.53 keV. Fluorescent radiation is also detected from C-K_alpha at 0.28 keV and, marginally, from N-K_alpha at 0.40 keV. An additional emission line is indicated at 0.29 keV, which might be the signature of the C 1s --> pi* transition in CO_2 and CO. Evidence for temporal variability of the X-ray flux was found at the 2.6 sigma level, with fluctuations by factors of a few times indicated on time scales of minutes. All these findings are fully consistent with fluorescent scattering of solar X-rays. No other source of X-ray emission was detected, in particular none from charge exchange interactions between highly charged heavy solar wind ions and atmospheric neutrals, the dominant process for the X-ray emission of comets. This is in agreement with the sensitivity of the observation.Comment: 12 pages, 9 figure

Structural design of the DIII-D radiative divertor

The divertor of the DIII-D tokamak is being modified to operate as a slot type, dissipative divertor. This modification, called the Radiative Divertor Program (RDP) is being carried out in two phases. The design and analysis is complete and hardware is being fabricated for the first phase. This first phase consists of an upper divertor baffle and cryopump to provide some density control for high triangularity, single or double null discharges. Installation of the first phase is scheduled to start in October, 1996. The second phase provides pumping at all four divertor strike points of double null high triangularity discharges and baffling of the neutral particles from transport back to the core plasma. Studies of the effects of varying the slot length and width of the divertor can be easily accomplished with the design of RDP hardware. Static and dynamic analyses of the baffle structures, new cryopumps, and feedlines were performed during the preliminary and final design phases. Disruption loads and differential thermal displacements must be accommodated in the design of these components. With the full RDP hardware installed, the plasma current in DIII-D will be a maximum of 3.0 MA. Plasma disruptions induce toroidal currents in the cryopump, producing complex dynamic loads. Simultaneously, the vacuum vessel vibrations impose a sinusoidal base excitation to the supports for the cryopump. Static and dynamic analyses of the cryopump demonstrate that the stresses due to disruption and thermal loadings satisfy the stress and deflection criteria

Applying brand management to higher education through the use of the Brand Flux Model™– the case of Arcadia University.

Within an increasingly more competitive landscape, Higher Education Institutions (HEIs) are becoming more marketized and promotionalized. Brand building is becoming a strategic administrative goal, yet clear brand management models are lacking. This paper utilizes the Brand Flux Model™ to assist in tracking the fluxing nature or historical patterns of branding practices, and provides a graphic representation for following changes in branding or changes in position that result in either Reinforcing an existing brand, or Revitalizing, Refocusing, Renaming, or Retiring a brand. Through a case analysis of an HEI that eventually underwent a radical renaming, the various phases of the Brand Flux Model™ are explored and the critical aspect of ongoing brand management efforts is reinforced. The paper also highlights why periodic brand audits are necessary to ascertain that what the institution believes it is promoting and projecting is consistent with the actual brand image held by stakeholders, and suggests that benchmarking brand management efforts and correlating them with the stage and actions of the Brand Flux Model™ can assist in understanding branding as a growth platform for service organizations. For practitioners, this study provides a model to assist in brand management and renaming scenarios, and offers insight into channels for optimal corporate strategy. It demonstrates that making changes in branding or changes in position in order to Revitalize, Refocus (rebrand and reposition) or even Rename a brand, and then Reinforce those decisions, is critical to maintaining brand health

Evolutionary conservation and in vitro reconstitution of microsporidian iron–sulfur cluster biosynthesis

This work was supported by Marie Curie Postdoctoral Fellowships to T.A.W., E. H. and S. L., a European Research Council Advanced Investigator Grant (ERC-2010-AdG-268701) to T.M.E., and a Wellcome Trust Programme Grant (number 045404) to T.M.E. and J.M.L. R.L. acknowledges generous financial support from Deutsche Forschungsgemeinschaft (SFB 593, SFB 987, GRK 1216, LI 415/5), LOEWE program of state Hessen, Max-Planck Gesellschaft, von Behring-Röntgen StiftungMicrosporidians are a diverse group of obligate intracellular parasites that have minimized their genome content and simplified their sub-cellular structures by reductive evolution. Functional studies are limited because we lack reliable genetic tools for their manipulation. Here, we demonstrate that the cristae-deficient mitochondrion (mitosome) of the microsporidian Trachipleistophora hominis is the functional site of iron-sulphur cluster (ISC) assembly, which we suggest is the essential task of this organelle. Cell fractionation, fluorescence imaging and fine-scale immunoelectron microscopy demonstrate that mitosomes contain a complete pathway for [2Fe-2S] cluster biosynthesis that we biochemically reconstituted using purified recombinant mitosomal ISC proteins. Reconstitution proceeded as rapidly and efficiently as observed for yeast or fungal mitochondrial ISC components. Core components of the T. hominis cytosolic iron-sulphur protein assembly (CIA) pathway were also identified including the essential Cfd1-Nbp35 scaffold complex that assembles a [4Fe-4S] cluster as shown by spectroscopic methods in vitro. Phylogenetic analyses reveal that both the ISC and CIA biosynthetic pathways are predominantly bacterial, but their cytosolic and nuclear target Fe/S proteins are mainly archaeal. This mixed evolutionary history of the Fe/S-related proteins and pathways, and their strong conservation among highly reduced parasites, provides additional compelling evidence for the ancient chimeric ancestry of eukaryotes.Publisher PDFPeer reviewe