Avaliação da casca de mamona na alimentação de ovinos.

Resumo: Objetivou-se com este trabalho, avaliar a casca de mamona na alimentação de ovinos. Foram utilizadas quatro fêmeas ovinas jovens, sem raça definida, com peso vivo médio de 30 kg e idade média de sete meses. Os animais foram mantidos em gaiolas de metabolismo e distribuídos em um delineamento em quadrado latino 4 x 4 para avaliar o efeito de quatro níveis de inclusão da casca de mamona em substituição ao milho em dietas isoprotéicas e isoenergéticas. Como alimento volumoso foi utilizado o feno de capim-Tifton, mantendo uma relação volumoso:concentrado de 60:40. Cada período experimental teve duração de 21 dias, sendo 14 dias de adaptação e sete dias de coleta de dados. Foram mensuradas as seguintes variáveis: a composição bromatológica e o valor de energia da casca de mamona, o consumo voluntário, a digestibilidade da matéria seca e de nutrientes e o balanço de nitrogênio. Houve uma redução no consumo da matéria seca e na digestibilidade da matéria orgânica com o aumento na participação da casca de mamona (P < 0,05). Com a substituição de 72,92% do milho por casca de mamona, houve também uma redução significativa da digestibilidade da fibra (P < 0,05). Não houve efeito dos tratamentos sobre o balanço de nitrogênio (P < 0,05), nem sintomas de intoxicação nos animais. A casca de mamona com 13% de sementes em sua composição, não apresentam efeitos tóxicos em níveis de até 15,25% da matéria seca da dieta, sendo um alimento com potencial de uso em dietas para pequenos ruminantes. [Castor bean hulls in the female sheep diets]. Abstract - This experiment was assigned to evaluate the castor bean hulls in the sheep feeding Four 7 month old young females, weighting 30 kg were used. They were set in metabolic cages and distributed in a 4 x 4 Latin Square design to evaluate the effect of four levels of castor bean hulls inclusion in substitution of corn grain diets. Bermudagrass hay was the forage used, maintaining a roughage:concentrate ratio 60:40. Each experimental period had duration of 21 days, being 14 days of adaptation and 7 days of data collection. The variables evaluated were: bromatologic composition and energy value of castor bean hulls, voluntary feed intake, dry matter and nutrients digestibility and nitrogen balance. There was a reduction in dry matter intake and in digestibility of organic matter with the increasing participation of castor bean hulls (P < 0.05). When the substitution from corn grain to castor bean hulls achieved 72.92%, there was also a significant reduction in fiber digestibility (P < 0.05). On the other hand, there was not effect of the treatments on nitrogen balance (P < 0.05) and did not occur intoxication symptoms in the animals. The castor bean hulls, with 13% of seed in their composition, did not present toxic effect in levels up to 15.25% of the dry matter, being a potential food source to be used in small ruminant diets

A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells

Background: Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs. Methods: We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors. Results: The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression. Conclusion: Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro

Effectiveness of different cellulose-based filtration materials against inhalation of SARS-CoV-2-like particles

The respiratory protection equipment (RPE) used by health professionals consists of an essential device to prevent infectious diseases, especially those caused by biological agents such as the coronavirus (SARS-CoV-2). The current epidemiological panorama is worrying, and the context of creation and production of the mask has emerged as an alternative to RPE to face the public health crisis worldwide. The aim of this work is to present a low-cost alternative as an FFP2-like filter for a reusable respirator face mask. This study presents the comparison of different cellulose-based filtering materials performed by retention testing, time saturation testing, aerosol penetration testing, nanoparticle (~140 nm) filtration testing, bacterial filtration efficiency (BFE), analysis of material morphology and usability. The reusable respirator face mask used in this study is an open-source innovation, using 3D printing. Cotton disc proved to be the best filter material for the reusable mask, with satisfactory results and a performance similar to that shown by the N95-type mask. The cotton disc ensured effectiveness over 6 h of use, and after that, the reusable respirator face mask (here, Delfi-TRON®) needed to be sanitized and replenished with a new cotton disc. Upon preliminary analyses of filtration efficiency, the selected filter was shown to be a low-cost biodegradable and biocompatible alternative.info:eu-repo/semantics/publishedVersio

Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair

The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model.The authors acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Brazil), Fundação de Amparo a Pesquisa do Estado de Sergipe (FAPITEC, MS/CNPq/FAPITEC/SE/SES N◦06/2018) e do Conselho Nacional de Pesquisas (CNPq, Apoio a Projetos de Pesquisa/MCTI/CNPQ/Universal 14/2014) for supporting funds. EBS acknowledges the Portuguese Science and Technology Foundation (FCT) for the funded projects M-ERA-NET/0004/2015 (PAIRED) and UIDB/04469/2020 (strategic fund) financed from national funds, and co-financed Education (FCT/MEC) from national funds and FEDER, under the Partnership Agreement PT2020.info:eu-repo/semantics/publishedVersio

β-cyclodextrin/isopentyl caffeate inclusion complex: synthesis, characterization and antileishmanial activity

Isopentyl caffeate (ICaf) is a bioactive ester widely distributed in nature. Our patented work has shown promising results of this molecule against Leishmania. However, ICaf shows poor solubility, which limits its usage in clinical settings. In this work, we have proposed the development of an inclusion complex of ICaf in -cyclodextrin (-CD), with the aim to improve the drug solubility, and thus, its bioavailability. The inclusion complex (ICaf:-CD) was developed applying three distinct methods, i.e., physical mixture (PM), kneading (KN) or co-evaporation (CO) in different molar proportions (0.25:1, 1:1 and 2:1). Characterization of the complexes was carried out by thermal analysis, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and molecular docking. The ICaf:-CD complex in a molar ratio of 1:1 obtained by CO showed the best complexation and, therefore, was selected for further analysis. Solubility assay showed a marked improvement in the ICaf:-CD (CO, 1:1) solubility profile when compared to the pure ICaf compound. Cell proliferation assay using ICaf:-CD complex showed an IC50 of 3.8 and 2.7 µg/mL against L. amazonesis and L. chagasi promastigotes, respectively. These results demonstrate the great potential of the inclusion complex to improve the treatment options for visceral and cutaneous leishmaniases.This research was funded by Banco do Nordeste (grant FUNDECI/2016.0015), Coordenação Aperfeiçoamento de Pessoal de Nivel Superior (CAPES) and Fundação de Ámparo à Pesquisa do Estado de Sergipe (FAPITEC) (PROCESSO: 88887.159533/2017-00 extração, encapsulação e caracterização de bioativos para o interesse biotecnologico). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 301964/2019-0 Chamada 06/2019, and Chamada CNPq nº 01/2019) and from the Portuguese Science and Technology Foundation (FCT) project UIDB/04469/2020 (strategic fund).info:eu-repo/semantics/publishedVersio

BRS Energia.

bitstream/CNPA/19902/1/Folder_brs_energia.pd

O antes e o depois do PIBID-Química: o papel na formação inicial de licenciandos do curso de Química da UFRN

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Curta Química: uma proposta para abordar conceitos químicos

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