Uma abordagem municipal sobre o fenómeno da Religião

Revista Lusófona de Ciência das Religiõe

O GARSE (Gabinete de Assuntos Religiosos e Sociais Específicos) da Câmara Municipal de Loures

O GARSE(Gabinete de Assuntos Religiosos e Sociais Específicos)da Câmara Municipalde Loure

Material Didático De Educação A Distância, Neoliberalismo E Autonomia: Relações (Im)Possíveis

In this work, we take a discursive perspective to discuss the representations of student in teaching materials used in higher distance education. We also reflect on the relationships between the meaning-effects of building an image of an autonomous learner, by the authors of these materials, and the neoliberal socio-historical context.Key words: autonomy, distance education, neoliberalism, discourse, representation.Neste trabalho, discutimos, a partir de um olhar discursivo, representações de aluno em materiais didáticos utilizados no ensino superior a distância. Também desenvolvemos uma reflexão sobre as relações entre os efeitos de sentido da construção de uma imagem de aprendiz autônomo, por autores desses materiais, e o contexto sóciohistórico neoliberal.Palavras-chave: autonomia, educação a distância, neoliberalismo, discurso, representação

Material Didático De Educação A Distância, Neoliberalismo E Autonomia: Relações (Im)Possíveis

In this work, we take a discursive perspective to discuss the representations of student in teaching materials used in higher distance education. We also reflect on the relationships between the meaning-effects of building an image of an autonomous learner, by the authors of these materials, and the neoliberal socio-historical context.Key words: autonomy, distance education, neoliberalism, discourse, representation.Neste trabalho, discutimos, a partir de um olhar discursivo, representações de aluno em materiais didáticos utilizados no ensino superior a distância. Também desenvolvemos uma reflexão sobre as relações entre os efeitos de sentido da construção de uma imagem de aprendiz autônomo, por autores desses materiais, e o contexto sóciohistórico neoliberal.Palavras-chave: autonomia, educação a distância, neoliberalismo, discurso, representação

Entomopathogenic fungi biomass production and extracellular biosynthesis of silver nanoparticles for bioinsecticide action

Entomopathogenic fungi are microbial agents of insect control in nature. They have been used as biologic strategies to manage insect invasion; however, the challenge is to maintain their shelf life and viability when exposed to high temperatures, ultraviolet radiation, and humidity. Synthesized silver nanoparticles (AgNPs) from fungal extracellular enzymes are an alternative using these microorganisms to obtain nanoparticles with insecticidal action. The present study evaluates the biomass production and the potential to synthesize silver nanoparticles using entomopathogenic fungi isolates. Sixteen isolates of entomopathogenic fungi were used in this study. The fungi pathogenicity and virulence were evaluated using the insect model Tenebrio molitor, at a concentration of 5 × 106 conidia/mL. The fungal biomass was produced in a liquid medium, dried, and weighed. The synthesis of silver nanoparticles was performed with aqueous extracts of the entomopathogenic fungi and silver nitrate solution (1 mM), following characterization by a UV/vis spectrophotometer, mean size, and polydispersity index. The results showed a significant variation in pathogenicity, virulence, and biomass production among the evaluated fungi isolates; however, only one of the isolates did not have the potential to synthesize silver nanoparticles. Pearsons correlation showed significant correlation values only between virulence × biosynthesis potential and biomass production × biosynthesis potential, both with negative values, indicating an inverse correlation. Thus, AgNPs with entomopathogenic fungus extract can produce an innovative bioinsecticide product using a green production process.This work was supported by the Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, #443238/2014-6, #470388/2014-5) and Banco do Nordeste (FUNDECI/2017.0014), the Portuguese Science and Technology Foundation (FCT/MCT), and from European Funds (PRODER/COMPETE) for the project UIDB/04469/2020 (strategic fund), co-financed by FEDER, under the Partnership Agreement PT2020.info:eu-repo/semantics/publishedVersio

A ESCRITA NO MSN:ALGO NOVO DEBAIXO DO SOL?

ABSTRACT – This paper aims to investigate the writing configuration of conversations in MSN Messenger and Gmail. We intend to show two effects of meanings of the new technologies of communication in the web surfer writing: of those who let themselves be “crossed” by the monitor and of those who present a relative distance from the writing, but they are still strongly “crossed” by the paper

Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC

AbstractDidanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28°C. For an isocratic run, the flux was 1.0mL/min, detection was at 250nm and injected volume was 20μL. The method was selective and linear for concentrations between 50 and 5000ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at −20°C for one month after three freeze–thaw cycles, and for 24h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs

Nanopesticides in agriculture: benefits and challenge in agricultural productivity, toxicological risks to human health and environment

Nanopesticides are nanostructures with two to three dimensions between 1 to 200 nm, used to carry agrochemical ingredients (AcI). Because of their unique properties, the loading of AcI into nanoparticles offers benefits when compared to free pesticides. However, with the fast development of new engineered nanoparticles for pests control, a new type of environmental waste is being produced. This paper describes the nanopesticides sources, the harmful environmental and health effects arising from pesticide exposure. The potential ameliorative impact of nanoparticles on agricultural productivity and ecosystem challenges are extensively discussed. Strategies for controlled release and stimuli-responsive systems for slow, sustained, and targeted AcI and genetic material delivery are reported. Special attention to different nanoparticles source, the environmental behavior of nanopesticides in the crop setting, and the most recent advancements and nanopesticides representative research from experimental results are revised. This review also addresses some issues and concerns in developing, formulating and toxicity pesticide products for environmentally friendly and sustainable agriculture.FDA—Sorocaba University; PROPEIN 013_19info:eu-repo/semantics/publishedVersio

use of rumination and activity data as health status and performance indicators in beef cattle during the early fattening period

Abstract The aim of this study was to measure the level of activity and rumination in young bulls and to assess whether these data can be used as indicators of health status and average daily weight gain (ADG). Two groups of animals (period 1: n = 108 animals; period 2: n = 106 animals) were fitted with sensors to measure daily activity and rumination, were weighed on arrival and at the end of the trial (70 days) and were checked twice daily to verify their health condition. Any clinical signs and therapies were recorded. The dishomogeneity index of rumination (DR), and the daily dishomogeneity indices of activity (DDA) and rumination (DDR), were calculated. Bulls had an ADG of 1.42 ± 0.38 kg/day and showed an average duration of daily rumination of 404 ± 63 min and an average activity of 474 ± 46 bits, respectively. Animals characterised by low ADG had lower values of minimum daily rumination ( P = 0.01) and DDA ( P 0.001), and a greater rumination range ( P = 0.007) and DR ( P = 0.003). Bovine respiratory disease and lameness were detected 31 and five times, respectively; among affected animals, the average daily activity, rumination and DDA were lower ( P 0.05) at 3–6 days before the onset of visible clinical signs, whereas DDR increased compared to the values when individuals were apparently healthy. The use of individual sensors appears promising for the early diagnosis of disease in beef cattle and for improving herd management

Are nanobiosensors an improved solution for diagnosis of Leishmania?

Leishmaniasis is one of the deadliest neglected tropical diseases affecting 1215 million people worldwide, especially in middle- and low-income countries. Rapid and accurate diagnosis of the disease is important for its adequate management and treatment. Several techniques are available for the diagnosis of leishmaniasis. Among these, parasitological and immunological tests are most widely used. However, in most cases, the utilized diagnostic techniques are not good enough, showing cross-reactivity and reduced accuracy. In recent years, many new methods have been reported with potential for improved diagnosis. This review focuses on the diagnosis of Leishmania exploring the biosensors and nanotechnology-based options for their detection. New developments including the use of nanomaterials as fluorophores, fluorescence quenchers as reducing agents and as dendrimers for signal improvement and amplification, together with the use of aptamers to replace antibodies are described. Future research opportunities to overcome the current limitations on the available diagnostic approaches are also discussed.This work was supported by São Paulo Research Foundation (FAPESP) (2010/17.721-4), Portuguese Science and Technology Foundation (FCT) through the projects M-ERA-NET/0004/2015 (PAIRED) and UIDB/04469/2020 (strategic fund) funded by national funds, and co-financed Educa tion (FCT/MEC) from national funds and FEDER, under the Partnership Agreement PT202info:eu-repo/semantics/publishedVersio