Results from a randomized, controlled trial on efficacy and tolerability of a 5% Lidocaine-Medicated Plaster vs. Pregabalin in patients with Post-Herpetic Neuralgia (PHN) and Painful Diabetic Polyneuropathy (DPN) [poster]

No abstract available

Effects of HIV status and linguistic medium on the test performance of rural low-literacy adults: implications for neuropsychological test development in Zambia

Purpose of the study: The purpose of the study was to determine whether the familiar language (Chichewa version) could contribute to the early diagnosis of neurocognitive dysfunctions and develop a battery of locally valid tests capable of detecting early changes in the cognitive profile of neurocognitive dysfunctions among HIV positive patients.Research question one: What is the difference in performance between HIV negative and HIV positive individuals when they are subjected to the four verbal tests of the neuropsychological test battery using the English and Chichewa versions?Research question two: What is the interaction effect among the influences of HIV status, linguistic medium and gender on the four verbal tests of the neuropsychological test battery?Design: It was an experimental design that assessed the neuropsychological effects of HIV status and linguistic medium on the test performance. The Hopkins Verbal Learning Test (HVLT-R) for both immediate and delayed recall were used to test the verbal episodic memory from the Verbal Learning and Memory Recall Domain (Brandt and Benedict, 2001). Other tests included Animal and Action Naming. These tests were translated into Chichewa and administered to 28 HIV positive and 22 HIV negative rural low illiterate adults aged between 40 and 65 years.Results: On all the Neuropsychological tests administered, HIV positive respondents scored significantly lower than HIV negative respondents, and the mean scores on the English medium version were consistently lower than scores on the L1 (Chichewa) version across all tests and all groups.Conclusion: The study has shown that the primary language is best suited to test neurocognitive performance and especially when one is using test components that do not require reading or writing

The role of self-compassion and self-criticism in binge eating behaviour

Self-criticism and low self-compassion are implicated in the development and maintenance of binge eating. However, the association between these self-attitudes and binge eating symptoms remains unclear. Women with symptoms of Bulimia Nervosa (BN) or Binge Eating Disorder (BED) were randomised to either a self-compassion (n = 30) or self-critical rumination (n = 30) strategy following a negative mood induction. Responses to food cues (cue reactivity and affect) and calorie consumption in a ‘taste test’ were assessed. The self-compassion strategy was associated with a greater improvement in positive and negative affect following the negative mood induction. Despite the differential effects on mood, self-compassion and self-critical rumination led to similar self-reported food cravings and physiological reactivity to cues. However, participants in the self-compassion condition consumed significantly fewer calories, rated the consumed food as less pleasurable, and reported less desire to continue eating. The findings suggest that therapeutic strategies for cultivating self-compassion are associated with improved food-related self-regulation in the context of negative mood

Linking changes in pain severity to changes in other outcomes in patients with posttraumatic peripheral neuropathic pain treated with pregabalin [POSTER]

No abstract available

Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell

High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the structure of such biomolecular systems. However, existing systems are not optimized for the requirements of fibrous proteins. We have designed and built a low-volume (200 μL), low-wavelength (down to 180 nm), low-pathlength (100 μm), high-alignment flow-alignment system (couette) to perform ultraviolet linear dichroism studies on the fibers formed by a range of biomolecules. The apparatus has been tested using a number of proteins for which longer wavelength linear dichroism spectra had already been measured. The new couette cell has also been used to obtain data on two medically important protein fibers, the all-β-sheet amyloid fibers of the Alzheimer's derived protein Aβ and the long-chain assemblies of α1-antitrypsin polymers

Satisfaction, adherence and health-related quality of life with transdermal buprenorphine compared with oral opioid medications in the usual care of osteoarthritis pain

Background Osteoarthritis (OA) causes substantial pain and reduced health-related quality of life (HRQL). Although opioid analgesics are commonly used, the relative benefits of different opioids are poorly studied. Transdermal buprenorphine (TDB) offers an alternative to oral opioids for the treatment of moderate-to-severe chronic pain. This observational study of people with OA pain assessed satisfaction, HRQL and medication adherence. Methods Patients in the UK with self-reported knee and/or hip OA who had been receiving one or more of TDB, co-codamol (an oral paracetamol/codeine combination) and tramadol for at least 1 month completed an online or telephone questionnaire. Medication satisfaction scores, HRQL scores (Short-Form 36 [SF-36]), medication adherence (Morisky Medication Adherence Scale [MMAS™]), adverse events and treatment discontinuations were recorded. Linear and logistic regression models were used to compare the treatment effect of TDB with co-codamol or tramadol. Results Overall, 966 patients met the inclusion criteria; 701 were taking only one of the target medications (TDB: 85; co-codamol: 373; tramadol: 243). The largest age group was 50–59 years and 76.0 % of patients were female. The TDB group was younger, with more male patients, therefore the statistical models were adjusted for age and sex. Medication satisfaction scores were significantly higher in the TDB group than the other two groups (TDB vs. co-codamol: 3.56, 95 % confidence interval [CI] 1.90–6.68, p < 0.0001; TDB vs. tramadol: 3.22, 95 % CI 1.67–6.20, p = 0.0005). Physical Component Summary scores for HRQL and mean adherence were also higher in the TDB group, while Mental Component Summary HRQL scores were similar across the three groups. Conclusions Patients with knee and/or hip OA pain treated with TDB were more satisfied and more adherent with their medication, and reported higher Physical Component Summary HRQL scores than those treated with co-codamol or tramadol, although demographic differences were observed between groups

Results from a randomized, controlled trial on efficacy and tolerability of a 5% Lidocaine-Medicated Plaster vs. Pregabalin in patients with Post-Herpetic Neuralgia (PHN) and Painful Diabetic Polyneuropathy (DPN) [poster]

No abstract available

A Qualitative Exploration of Sport and Social Pressures on Elite Athletes in Relation to Disordered Eating

Introduction: Athletes are at increased risk of disordered eating compared to non-athletes. Inspired by previous investigation into quantitative work on an etiological model of disordered eating in athletes, the current study aimed to explore a problematic aspect of the model: athletes' lived experiences of social and sport pressures in relation to the onset of disordered eating and differing eating behaviors. / Methods: Nine (N = 9) male and female athletes representing a range of endurance sports took part in semi-structured interviews. Thematic analysis was utilized. / Analysis: Analysis revealed two main themes each with two corresponding subthemes (1) Conflating physical appearance and sporting ability with the subthemes of (1a) social comparison in a sporting world and (1b) societal notions of “the athlete body” and (2) Living as an athlete with the corresponding subthemes of (2a) discipline and sacrifice and (2b) the balancing act. / Discussion: It is the complex interaction between societal expectations as lived out in social messages and comparisons, and sport pressures that contributes to the development of disordered eating behaviors. These findings suggest that prevention and treatment of disordered eating in athletes can be applied from those already established in non-sporting realm

Selection of optimised ligands by fluorescence-activated bead sorting

The chemistry of aptamers is largely limited to natural nucleotides, and although modifications of nucleic acids can enhance target aptamer affinity, there has not yet been a technology for selecting the right modifications in the right locations out of the vast number of possibilities, because enzymatic amplification does not transmit sequence-specific modification information. Here we show the first method for the selection of specific nucleoside modifications that increase aptamer binding efficacy, using the oncoprotein EGFR as a model target. Using fluorescence-activated bead sorting (FABS), we have successfully selected optimized aptamers from a library of >65 000 variations. Hits were identified by tandem mass spectrometry and validated by using an EGFR binding assay and computational docking studies. Our results provide proof of concept for this novel strategy for the selection of chemically optimised aptamers and offer a new method for rapidly synthesising and screening large aptamer libraries to accelerate diagnostic and drug discovery

Sequence Isomerism in Uniform Polyphosphoesters Programmes Self-Assembly and Folding

We have adapted solid phase phosphoramidite synthesis commonly used to make DNA, to produce two sequence-isomeric non-biological polymers which display sequence-programmed folding and self-assembly, going beyond structures which would be trivially anticipated. These findings open up possibilities for more sophisticated sequence/structure relationships using the same synthetic platform