Bazı monoterpenoidlerin fungal biyotransformasyonunun incelenmesi

06.03.2018 tarihli ve 30352 sayılı Resmi Gazetede yayımlanan “Yükseköğretim Kanunu İle Bazı Kanun Ve Kanun Hükmünde Kararnamelerde Değişiklik Yapılması Hakkında Kanun” ile 18.06.2018 tarihli “Lisansüstü Tezlerin Elektronik Ortamda Toplanması, Düzenlenmesi ve Erişime Açılmasına İlişkin Yönerge” gereğince tam metin erişime açılmıştır.Monoterpenoidlerin biyotransformasyonları özellikle gıda sanayi, parfümeri ve eczacılıkta kullanılan çok önemli bileşiklerin hazırlanması amacıyla dünya çapında yaygın olarak uygulanmaktadır. Birçok monoterpenoid yıllardır bu amaçla mikrobiyal biyotransformasyonlara maruz bırakılmaktadır. Günümüzde mikrobiyal biyotransformasyonların etkinliğinin artırılması ve faydalı olabilecek yeni mikroorganizmalar ile reaksiyonların bulunmasına yönelik yoğun çalışmalar sürmektedir.Bu çalışmada (-)-mirtenol, (-)-nopol, (-)-verbenon, (±)-?-iyonon ve ß-iyonon monoterpenoidlerinin Aspergillus tamarii, Aspergillus terreus ve Aspergillus fumigatus küfleri ile biyotransformasyonları gerçekleştirildi. Aspergillus tamarii ve Aspergillus terreus ile inkübasyonlar bazı hidroksillenmiş metabolitler verirken Aspergillus fumigatus ile inkübasyonlar sadece değişmeyen başlangıç maddelerini verdi.Metabolitlerin yapısı optik rotasyonlarının ölçülmesi, 1H NMR, 13C NMR, 13C DEPT NMR ile FTIR spektrumlarının alınması ve bunların başlangıç maddelerininkilerle karşılaştırılması ile belirlendi.Microbial biotransformations of monoterpenoids have found worldwide application for the production of more valuable and functionalized compounds used especially in foods, perfumes and medicines. A number of researches on microbial biotransformations of a wide range of monoterpenoids have been reported for years. There are still enormous efforts to increase the efficiency of microbial biotransformations and find new useful microorganisms and reactions.In this work, the incubations of (-)-myrtenol, (-)-nopol, (-)-verbenone, (±)-?-ionone and ß-ionone were performed with Aspergillus tamarii, Aspergillus terreus and Aspergillus fumigatus. Incubations with Aspergillus tamarii and Aspergillus terreus afforded some hydroxylated metabolites whereas incubations with Aspergillus fumigatus afforded unchanged starting materials only.The structures of metabolites were determined by measuring their optical rotations, taking their 1H NMR, 13C NMR, 13C DEPT NMR and FTIR spectra and comparing them with those of starting materials

Can We Trust a Compact Bacteriological Screening Test to Identify the Common Vaginal Pathogens?

Objectives: The objective of this study was to evaluate the performance of the A.F. Genital System® in the detection of vaginal pathogens in patients with obstetrical and gynecological pathologies. Materials and Methods: A total of 197 vaginal swab samples were collected from patients presenting with various obstetrical and gynecological pathologies. The A.F. Genital System® and vaginal culture/traditional methods were used for pathogen detection. Results: The A.F. Genital System® demonstrated a detection rate of 68% for single vaginal infectious agents, outperforming the vaginal culture/traditional methods (52.8%). However, differences in detection rates were observed for specific pathogens, such as E. coli, Gardnerella vaginalis, Staphylococcus aureus, and Pseudomonas spp. Conclusions: Despite lower sensitivity for specific pathogens, the A.F. Genital System® showed a high correlation with reference tests, suggesting its potential utility as a diagnostic tool for identifying common vaginal pathogens in clinical settings

Renal complications of lipodystrophy: A closer look at the natural history of kidney disease

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144612/1/cen13732_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144612/2/cen13732.pd

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

18 üyeli N2O2S2 hetero atomlu makrosiklik oksim sentezi ve komplekslerinin incelenmesi

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır.ÖZET Anahtar kelimeler: vic-Dioksim, katekol, siyanojen-di-N-oksit, Ni11, Cou, Cu11, UO2ÇİÎ) compleksleri Koordinasyon bileşikleri içerisinde oksim ve vic-dioksimlerden elde edilen kompleksler ayrı bir önem taşır. Oksim adı oksi ve imin kelimelerinin birleşmesiyle oluşmuştur (-C= NOH). Oksim grubu zayıf bazik azot atomu ve orta şiddette asidik hidroksil grubu dolayısıyla amfiprotik bir özellik taşır. Oksim bileşikleri; şelat oluşturma, oksijen tutma, biyolojik olarak kendiliğinden parçalanabilme gibi özellikleri ve fotokimyasal reaksiyonlardaki eşsiz etkileri ile geniş ölçüde tanınmaktadır. Birçok sentezlerde kullanılmaları yanında geçiş elementleriyle sağlam kompleksler yaparlar. Oksimler aldehit ve ketonlar ile hidroksil aminlerin kondenzasyon ürünleridir. Bu çalışmanın amacı vic-dioksimlerde halkayı oluşturarak üç tür hetero atomu N2S2O2 şeklinde biraraya getirmektir. Bu maksatla; bu çalışmada literatürden yararlanılarak başlangıç maddesi olarak 1,2- bis (2-Iyodoetoksi) 4,5-dibromobenzen bileşiği 4 kademede katekolden başlıyarak sentezlenmiş ve yüksek verim elde edilmiştir (%75). Bu maddenin (1,2- dihidroksibenzen) 2-aminotiyofenol ile kondenzasyonundan l,2-bis(2'- aminofenilsülfonil etoksi) 4,5-dibromo benzen elde edilmiştir.Bu kademeden sonra üç farklı hetero atom içeren (N2S2O2) bu yapıdan (l,2-bis(2'-aminofenilsülfonil etoksi) 4,5-dibromo benzen), siyanojen di-N-oksit ile -40°C deki muamelesinden literatürde bulunmayan orjinal makrosiklik oksim bileşiğine. [2,3-Dibromo- 6,7,13,14,15,16,22,23 oktahidro 14,15-bis (hidroksiimino) tribenzo[e,k,q] [1,4,7,10,13,16] diazodioksaditiyasiklooktadesin] geçilmiştir. Son aşama olarak elde edilen bu makrosiklik oksimden Nikel(II), Kobalt(II), Bakır(II) iyonlan ile metal ligand oranı 1:2 olan mononükleer, Uranil(U02) ile metal ligand oranı 2:2 olan binükleer kompleksleri izole edilmiştir. Sentezi yapılan bu yeni maddelerin yapılan elementel analiz, UV/VIS, İR, ^-NMR ve MS teknikleriyle aydınlatılmıştır. vıııSYNTHESIS AND COMPLEX FORMATION OF NOVEL VIC- DIOXIMES KEYWORDS: vic-Dioximes, macrocyclic compounds, cyanogen-di-N-oxide, Niu, Co11, Cun, U02(n) complexes. The transition metal complexes of macrocyclic ligands have been subject of great interest to researchers in catalysis, enzyme, functions and the other areas of the chemistry. Vicinal dioximes have eceived consideable attention as model compounds to mimic bi function such as reduction of vitamin B12. Their complexes have been the source, throguh the decades, of a never-ending series of interesting reports. The oxime groups is derived from oxy-imine, C=NOH. This group is amphiprotic with a slightly basic nitrogen and mildly acidic hydoxyl-group owing to their importance as stable MN4 core-containing coordination compounds, vie dioxime complexes have been much invertigated. incorporating of vic-dioksime moiety on the macrocycle provides can efficient binding site for transition metal ions by formation of on MN4 core with additions two hydrogen bridges. As a ligand, the oxime group is potentially amphidedante with possibilites of coordination through nitrogen sulpher and/or oxygen atoms. The best known example of a vic-dioxime is dimethylglioxime (H2DMG), which gives the well- known red nickel complexes with Ni+2 in ammoniacal solution. The detailed stuructures of a fairly large of metal vic-dioximates are known from X-ray investigations. The important features apart from N4 planar binding are the strong O H O hydrogen bondings and stacking of the planar units to each other in the crystal, in the case of Niu and Pdu in general. Our primary aim has been synthesized a new macrocycle vic-dioxime bearing higher member of macrocycles on the periphery. Oxime complexes carrying three kind donor atoms, such as N, O, S in the higher member macrocycles are rather few or almost never. Acrording to this strategy we have in report the synthesis of a new vic-dioksime coming together on the some ligand molecule. For this purpose, the synthetic work started of the known-compounds with catechol and finalized with 1,2-bis (2- iyodoetoxy) 4,5-dibromobenzene. The first orginal step is the synthesis of 1,2-bis i

The Preparation of Zeolite/Ag Composite Powders by Electroless Deposition Process

Core-shell Ag-coated zeolite composite powders were synthesized using silver electroless deposition process, which was carried out in an ammonia-based coating solution containing silver nitrate as a precursor material. The influence of the chemical components and powder concentration in the Ag coating was investigated by scanning electron microscopy, energy dispersive spectroscopy, and X-ray diffraction techniques. The zeolite/Ag composite powders were fabricated in order to find an antibacterial material and a new implant material based on the microstructural change of zeolite/Ag composites powders using different parameters of Ag deposition

Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads

In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Ammonia is produced from the enzymatic reactions between Gly and GDH with NAD(+) in phosphate buffer medium. The indophenol blue method was used for ammonia detection based on the spectrophotometric measurements of blue-colored product absorbing at 640 nm. The calibration graph is linear in the range of 0.1-10 mM of Gly concentrations. The effect of pH, temperature, and time interval was studied to find column stability, and also the interference effects of other amino acids was investigated. The interaction between GDH and glyoxal agarose beads was analyzed by Fourier transform infrared (FTIR) spectroscopy. The morphology of the immobilized and non-immobilized agarose beads were characterized by atomic force microscopy (AFM)

Biosorption of cadmium, manganese, nickel, lead, and zinc ions by Aspergillus tamarii

In this paper, Cd2+, Mn2+, Ni2+, Pb2+, and Zn2+ heavy metals ions adsorption properties of commercially obtained Aspergillus tamarii were investigated. The dead biomass was used with a batch system for experiments. The effect of the operating parameters, such as pH, temperature, agitation speed, contact time, initial metal concentration, and biomass dosage of aqueous solution containing Cd2+, Mn2+, Ni2+, Pb2+, and Zn2+ was studied to find biosorption capacity. The optimum pH range for all heavy metal uptakes was 6.0. The experiments were carried out at different temperatures in the range of 20-50 degrees C and the maximum uptake was found to be at 25 degrees C. Heavy metal ion uptake increased with agitation speed until 150rpm. After this agitation speed adsorption capacity slightly decreased. The adsorption equilibrium was obtained at 150min contact time. At the optimal conditions, maximum uptake of Cd2+, Mn2+, Ni2+, Pb2+, and Zn2+ was found to be 51.69, 46.99, 58.74, 98.14, and 54.33%, respectively, by using 1.5g biomass. The interaction between heavy metals and biomass was characterized by FTIR spectroscopy

Fatty acid composition of root and shoot samples of some Astragalus L. (Fabaceae) taxa growing in the east and southeast of Turkey.

The fatty acid compositions of root and shoot samples of some Astragalus L. (Fabaceae) taxa [A. gossypinus Fisch., A. amblolepis Fisch., A. edmondi (Kuntze) Sheld., A. tigridis Boiss., A. aleppicus Boiss., A. suberosus Banks & Sol. subsp. suberosus Banks & Sol., A. gummifer Labill., A. diphtherites Fenzl var. diphtherites Fenzl, and A. gymnalopecias Rech.f.] growing in the east and southeast of Turkey were determined by gas chromatography. Astragalus species contained palmitic (C 16:0) (20.13%–53.8%), linoleic (C 18:2ω6) (13.25%–41.06%), oleic (C 18:1ω-9) (5.78%–25.7%), linolenic (C 18:3ω-3) (6.1%–22.89%), and stearic acid (C 18:0) (5.16%–13.1%) in the roots and linolenic (8.9%–51.42%), palmitic (20.16%–44.88%), linoleic (7.34%–27.57%), oleic (2.45%–17.91%), and stearic acid (4.28%–8.31%) in the shoots as major components. In this study the fatty acid composition of Astragalus species showed uniform fatty acid patterns. Palmitic and stearic acids were the major saturated and linoleic and linolenic acids were the major unsaturated fatty acids in the roots and shoots