Investigating Cepheid ℓ\ell Carinae's Cycle-to-cycle Variations via Contemporaneous Velocimetry and Interferometry

Baade-Wesselink-type (BW) techniques enable geometric distance measurements of Cepheid variable stars in the Galaxy and the Magellanic clouds. The leading uncertainties involved concern projection factors required to translate observed radial velocities (RVs) to pulsational velocities and recently discovered modulated variability. We carried out an unprecedented observational campaign involving long-baseline interferometry (VLTI/PIONIER) and spectroscopy (Euler/Coralie) to search for modulated variability in the long-period (P $\sim$ 35.5 d) Cepheid Carinae. We determine highly precise angular diameters from squared visibilities and investigate possible differences between two consecutive maximal diameters, $\Delta_{\rm{max}} \Theta$. We characterize the modulated variability along the line-of-sight using 360 high-precision RVs. Here we report tentative evidence for modulated angular variability and confirm cycle-to-cycle differences of $\ell$ Carinae's RV variability. Two successive maxima yield $\Delta_{\rm{max}} \Theta$ = 13.1 $\pm$ 0.7 (stat.) {\mu}as for uniform disk models and 22.5 $\pm$ 1.4 (stat.) {\mu}as (4% of the total angular variation) for limb-darkened models. By comparing new RVs with 2014 RVs we show modulation to vary in strength. Barring confirmation, our results suggest the optical continuum (traced by interferometry) to be differently affected by modulation than gas motions (traced by spectroscopy). This implies a previously unknown time-dependence of projection factors, which can vary by 5% between consecutive cycles of expansion and contraction. Additional interferometric data are required to confirm modulated angular diameter variations. By understanding the origin of modulated variability and monitoring its long-term behavior, we aim to improve the accuracy of BW distances and further the understanding of stellar pulsations.Comment: Accepted for publication in MNRAS. 19 pages, 13 figures, 10 table

Be Stars: Rapidly Rotating Pulsators

I will show that Be stars are, without exception, a class of rapidly rotating stars, which are in the majority of cases pulsating stars as well, while none of them does possess a large scale (i.e. with significant dipolar contribution) magnetic field.Comment: Review talk given at "XX Stellar Pulsation Conference Series: Impact of new instrumentation and new insights in stellar pulsations", Granada, 5-9 September 2011, in press in AIP Conf. Se

Implementation and Analysis of Respondent Driven Sampling: Lessons Learned from the Field

Those who engage in illegal or stigmatized behaviors, which put them at risk of HIV infection, are largely concentrated in urban centers. Owing to their illegal and/ or stigmatized behaviors, they are difficult to reach with public health surveillance and prevention programs. 1 These populations include illicit drug users, sex workers and men who have sex with men. Development and implementation of adequate prevention services targeting hidden populations requires data on risk behaviors and disease prevalence from non-biased samples. In the last two decades, a number of sampling methods have been used to collect risk behavior and disease prevalence data from highly at-risk populations and to direct survey participants to prevention services. These include venue-based time–space sampling, targeted sampling, and snowball sampling. Time–space (also called time–location or venue–day–time) and targeted sampling provide coverage limited to population members who are readily accessible; those who are missed may differ from those who are Bcaptured[. 2 Targeted sampling fares well when compared to other forms of convenienc

Lack of functional GABA receptors alters Kiss1 , Gnrh1 and Gad1 mRNA expression in the medial basal hypothalamus at postnatal day 4

Background/Aims: Adult mice lacking functional GABAB receptors (GABAB1KO) show altered Gnrh1 and Gad1 expressions in the preoptic area-anterior hypothalamus (POA-AH) and females display disruption of cyclicity and fertility. Here we addressed whether sexual differentiation of the brain and the proper wiring of the GnRH and kisspeptin systems were already disturbed in postnatal day 4 (PND4) GABAB1KO mice. Methods: PND4 wild-type (WT) and GABAB1KO mice of both sexes were sacrificed; tissues were collected to determine mRNA expression (qPCR), amino acids (HPLC), and hormones (RIA and/or IHC). Results: GnRH neuron number (IHC) did not differ among groups in olfactory bulbs or OVLT-POA. Gnrh1 mRNA (qPCR) in POA-AH was similar among groups. Gnrh1 mRNA in medial basal hypothalamus (MBH) was similar in WTs but was increased in GABAB1KO females compared to GABAB1KO males. Hypothalamic GnRH (RIA) was sexually different in WTs (males < females), but this sex difference was lost in GABAB1KOs; the same pattern was observed when analyzing only the MBH, but not in the POA-AH. Arcuate nucleus Kiss1 mRNA (micropunch-qPCR) was higher in WT females than in WT males and GABAB1KO females. Gad1 mRNA in MBH was increased in GABAB1KO females compared to GABAB1KO males. Serum LH and gonadal estradiol content were also increased in GABAB1KOs. Conclusion: We demonstrate that GABABRs participate in the sexual differentiation of the ARC/MBH, because sex differences in several reproductive genes, such as Gad1, Kiss1 and Gnrh1, are critically disturbed in GABAB1KO mice at PND4, probably altering the organization and development of neural circuits governing the reproductive axis. (c) 2013 S. Karger AG, Basel

Application of Respondent Driven Sampling to Collect Baseline Data on FSWs and MSM for HIV Risk Reduction Interventions in Two Urban Centres in Papua New Guinea

The need to obtain unbiased information among hard–to-reach and hidden populations for behavioural and biological surveillance, epidemiological studies, and intervention program evaluations has led researchers to search for a suitable sampling method. One method that has been tested among IDU and MSM recently is respondent-driven sampling (RDS). We used RDS to conduct a behavioural survey among FSWs and MSM in two urban centres in Papua New Guinea (PNG). In this paper we present the lessons learned implementing RDS in a developing country setting. We also present comparisons of RDSAT-adjusted versus unadjusted crude estimates of some key socio-demographic indicators as well as comparisons between the estimates from RDS and a hypothetical time–location sample (TLS). Overall, the use of RDS among the MSM and FSWs in PNG had numerous advantages in terms of collecting a required sample size in a short time period, minimizing costs and maximising security for staff and respondents. Although there were a few problems these were easily remedied and we would recommend RDS for other similar studies in PNG and other developing countries

On the Binarity of LBV Stars

We report on the binarity of luminous blue variable stars observed with a set of techniques and instruments. Among them, observations at high angular resolution with the VLT-NACO, the VLTI-AMBER and with spectrographs such as the VLT-XSHOOTER allowed us to find several LBV stars as binaries or having a potential companion. In particular the LBV Pistol Star clearly presents radial velocity variations and line profiles modifications (double peak appearance). In addition, the absorption component of the P Cygni lines varies as well with the time indicating a potential wind structure variability. Our observations also show directly for the first time a companion to at least one of the observed LBVs (HD 168625). This one seems to affect the environment of the system. This system is known to be surrounded by several rings similar to those of SN1987A, possibly indicating a future supernova occurrence for this Galactic object. These results show that Eta Car is no longer unique

miR-346 controls release of TNF-alpha protein and stability of its mRNA in rheumatoid arthritis via tristetraprolin stabilization

TNF-alpha is a major cytokine implicated in rheumatoid arthritis. Its expression is regulated both at the transcriptional and posttranscriptional levels and recent data demonstrated that miRNAs are implicated in TNF-alpha response in macrophages. LPS-activated FLS isolated from RA patients express TNF-alpha mRNA but not the mature protein. This prompted us to look for miRNAs which could be implicated in this anti-inflammatory effect. Using a microarray, we found two miRNAs, miR-125b and miR-939 predicted to target the 3'-UTR of TNF-alpha mRNA, to be up-regulated in RA FLS in response to LPS, but their repression did not restore mature TNF-alpha expression in FLS. We showed previously that miR-346, which is upregulated in LPS-activated FLS, inhibited Btk expression that stabilized TNF-alpha mRNA. Blocking miR-346 reestablished TNF-alpha expression in activated FLS. Interestingly, transfection of miR-346 in LPS-activated THP-1 cells inhibited TNF-alpha secretion. We also demonstrated that TTP, a RNA binding protein which inhibited TNF-alpha synthesis, is overexpressed in activated FLS and that inhibition of miR-346 decreases its expression. Conversely, transfection of miR-346 in LPS-activated THP-1 cells increased TTP mRNA expression and inhibited TNF-alpha release. These results indicate that miR-346 controls TNF-alpha synthesis by regulating TTP expression

Respondent-Driven Sampling in Participatory Research Contexts: Participant-Driven Recruitment

This article reports on the use of respondent-driven sampling (RDS) in participatory and community-based research. Participant-driven recruitment (PDR) retains all of the analytic capabilities of RDS while enhancing the role of respondents in framing research questions, instrument development, data interpretation, and other aspects of the research process. Merging the capabilities of RDS with participatory research methods, PDR creates new opportunities for engaging community members in research addressing social issues and in utilizing research findings within community contexts. This article outlines PDR’s synthesis of RDS and participatory research approaches, describes how PDR is implemented in community contexts, and provides two examples of the use of PDR, illustrating its process, potentials, and challenges

The Gaia-ESO survey: A spectroscopic study of the young open cluster NGC 3293

We present a spectroscopic analysis of the GIRAFFE and UVES data collected by the Gaia-ESO survey for the young open cluster NGC 3293. Archive spectra from the same instruments obtained in the framework of the `VLT-FLAMES survey of massive stars' are also analysed. Atmospheric parameters, non-LTE chemical abundances for six elements, or variability information are reported for a total of about 160 B stars spanning a wide range in terms of spectral types (B1 to B9.5) and rotation rate (up to 350 km/s). We take advantage of the multi-epoch observations to detect several binary systems or intrinsically line-profile variables. A deconvolution algorithm is used to infer the current, true (deprojected) rotational velocity distribution. We find a broad, Gaussian-like distribution peaking around 200-250 km/s. Although some stars populate the high-velocity tail, most stars in the cluster appear to rotate far from critical. We discuss the chemical properties of the cluster, including the low occurrence of abundance peculiarities in the late B stars and the paucity of objects showing CN-cycle burning products at their surface. We argue that the former result can largely be explained by the inhibition of diffusion effects because of fast rotation, while the latter is generally in accord with the predictions of single-star evolutionary models under the assumption of a wide range of initial spin rates at the onset of main-sequence evolution. However, we find some evidence for a less efficient mixing in two quite rapidly rotating stars that are among the most massive objects in our sample. Finally, we obtain a cluster age of ~20 Myrs through a detailed, star-to-star correction of our results for the effect of stellar rotation. This is significantly older than previous estimates from turn-off fitting that fully relied on classical, non-rotating isochrones. [abridged]Comment: 29 pages, 24 figures, accepted for publication in A&

The Gaia-ESO Survey: Pre-Main Sequence Stars in the Young Open Cluster NGC 3293

The young open cluster NGC3293 is included in the observing program of the Gaia-ESO survey (GES). The radial velocity values provided have been used to assign cluster membership probabilities by means of a single-variable parametric analysis. These membership probabilities are compared to the results of the photometric membership assignment of NGC3293, based on UBV RI photometry. The agreement of the photometric and kinematic member samples amounts to 65%, and could increase to 70% as suggested by the analysis of the differences between both samples. A number of photometric PMS candidate members of spectral type F are found, which are confirmed by the results from VPHAS photometry and SED fitting for the stars in common with VPHAS and GES data sets. Excesses at mid- and near-infrared wavelengths, and signs of Hα emission, are investigated for them. Marginal presence of Hα emission or infilling is detected for the candidate members. Several of them exhibit moderate signs of U excess and weak excesses at mid-IR wavelengths. We suggest that these features originate from accretion disks in their last stages of evolution