EFEKTIVITAS IMPLEMENTASI SISTEM MANAJEMEN MUTU ISO 9001:2008 DI MAN 2 MODEL PEKANBARU

Penelitian ini berjudul efektivitas implementasi sistem manajemen mutu ISO 9001:2008 di MAN 2 Model Pekanbaru. Adapun tujuan penelitian ini adalah untuk mengetahui Efektivitas Implementasi Sistem Manajemen Mutu ISO 9001:2008 di MAN 2 Model Pekanbaru. Penelitian ini menggunakan pendekatan kualitatif dengan metode deskriptif. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dan studi dokumentasi, dengan peneliti sebagai instrument kunci. Teknik pengambilan sampel dalam penelitian ini menggunakan purposive sampling yaitu peneliti menunjuk secara langsung informan yang dianggap dapat memberikan informasi akurat. Uji keabsahan data dilakukan dengan uji reliabilitas dan validitas. Dari hasil analisis deskriptif dengan strategi triangulasi diperoleh pemenuhan klausul ISO 9001:2008 oleh MAN 2 Model Pekanbaru secara garis besar yaitu: 1) Penetapan perencanaan; 2) Pelaksanaan; 3) Pemeriksaan Proses; dan 4) Rencana tindak lanjut. Hasil penelitian ini menunjukkan bahwa implementasi sistem manajemen mutu ISO 9001:2008 sudah berjalan efektif. Hal ini dapat dilihat dari presentase tercapainya target sasaran-sasaran mutu yang telah ditetapkan sebelumnya, dari sembilan target yang ditentukan, hanya ada satu target sasaran mutu yang tidak dapat dicapai, dengan arti sekitar 90% target sasaran mutu dapat tercapai sesuai dengan jangka waktunya. Serta dari rekapitulasi hasil audit internal ditemukan bahwa presentase ketidaksesuaian mengalami penurunan setiap tahunnya. ;---This study, entitled the effectiveness of the implementation of the quality management system ISO 9001: 2008 in MAN 2 Model Pekanbaru. The purpose of this study was to determine the effectiveness of implementation of the Quality Management System ISO 9001: 2008 in MAN 2 Model Pekanbaru. This study used a qualitative approach with descriptive methods. Data collection techniques used were observation, interviews and documentation study, by researchers as a key instrument. The sampling technique in this study using purposive sampling that researchers pointed directly informant who was supposed to provide accurate information. Test the validity of the data is done with reliability and validity testing. Descriptive analysis of the results obtained by triangulation strategy fulfillment of the clauses of ISO 9001: 2008 by MAN 2 Model Pekanbaru outline namely: 1) Establishment of planning; 2) Implementation; 3) Examination Process; and 4) follow-up plan. The results of this study indicate that the implementation of the quality management system ISO 9001: 2008 has been running effectively. It can be seen from the percentage achieving the target goals established quality before, from nine targets set, there is only one target quality objectives can not be achieved, meaning about 90% of target quality objectives can be achieved in accordance with the time period. As well as of the recapitulation of the internal audit found that the percentage of non-conformity has decreased every year

Reflux precipitation polymerization : a new synthetic insight in molecular imprinting at high temperature

Synthesis of uniform molecularly imprinted polymer (MIP) microspheres (MSs) using distillation precipitation polymerization (DPP) at high temperature has attracted great interest in the field of molecular imprinting. However,there were still some shortcomings in this method. In this work, to create uniform MIP MSs in a short time and to demonstrate the effects of high temperature on imprinting performance, a new precipitation polymerization method (reflux precipitation polymerization, RPP) was used for the first time to fabricate MIP MSs in this study. The SEM images of the polymeric MSs indicate the presence of template molecules could improve the particle morphology and size uniformity. The specific molecular recognition of the monodispersed MIP MSs was confirmed by fluorescence measurement and HPLC-UV analysis. The binding behavior of the MIP MSs was simulated using the heterogeneous Freundlich isotherm, which shows that the MIP MSs produced by the RPP possess compatible selectivity in comparison with those by traditional PP method. It is noted that, for the first time, we demonstrated that molecular imprinting at high temperature was only successful when electrostatic interactions played important roles in the imprinting process

Développement d’une méthode de dosage automatisé du méthotrexate dans le plasma humain par couplage direct à la chromatographie liquide de l’extraction sur phase solide à empreintes moléculaires

Peer reviewe

Развитие инвестиционного кредитования в Украине

Free radical polymerization is often used to prepare protein and peptide-loaded hydrogels for the design of controlled release systems and molecular imprinting materials. Peroxodisulfates (ammonium peroxodisulfates (APS) or potassium peroxodisulfates (KPS)) with N,N,N,N-tetramethylethylenediamine (TEMED) are frequently used as initiator and catalyst. However, exposure to these free radical polymerization reagents may lead to modification of the protein and peptide. In this work, we show the modification of lysine residues by ammonium peroxodisulfate (APS)/TEMED of the immunostimulant thymopentin (TP5). Parallel studies on a decapeptide and a library of 15 dipeptides were performed to reveal the mechanism of modification. LC-MS of APS/TEMED-exposed TP5 revealed a major reaction product with an increased mass (+12 Da) with respect to TP5. LC-MS2 and LC-MS3 were performed to obtain structural information on the modified peptide and localize the actual modification site. Interpretation of the obtained data demonstrates the formation of a methylene bridge between the lysine and arginine residue in the presence of TEMED, while replacing TEMED with a sodium bisulfite catalyst did not show this modification. Studies with the other peptides showed that the TEMED radical can induce methyleneation on peptides when lysine is next to arginine, proline, cysteine, aspargine, glutamine, histidine, tyrosine, tryptophan, and aspartic acid residues. Stability of peptides and protein needs to be considered when using APS/TEMED in in situ polymerization systems. The use of an alternative catalyst such as sodium bisulfite may preserve the chemical integrity of peptides during in situ polymerization

Cover and uncover : chiral switching exploiting templating and layer-by-layer grafting

Consecutive grafting of crosslinked L-phenylalanine anilide, D-phenylalanine anilide and non-imprinted polymer layers on the surface of wide pore silica resulted in porous composite beads displaying enantiomeric discrimination reflecting the last grafted layer. This no longer holds for beads freed from silica, which instead display enantio-selectivity reflecting the first grafted layer

Insights into the formation, structural properties and performance of RAFT polymerized L-phenylalanine anilide molecularly imprinted polymers

Conventional molecularly imprinted polymers (MIPs) are amorphous materials with a nonuniform microenvironment and a broad distribution of binding sites. Controlled radical polymerization has been demonstrated to improve their properties in this regard. The RAFT method was employed in the present study with the aim of achieving more homogeneous MIP monolithic structures. RAFT control by α-cyanobenzyldithiobenzoate was employed during the synthesis of a poly(methacrylic acid-co-ethylene glycol dimethacrylate) conventional MIP for L-phenylalanine anilide. The influence of the amount of the added RAFT agent on the polymerization as well as on the polymer porous structure and properties was studied. The results demonstrate that the RAFT agent promoted retardation and a more gel-like polymer morphology as reflected in a decrease in pore diameter and an increased swelling factor with the added RAFT agent. This was accompanied by an enhanced thermal stability of up to 100 °C compared to a corresponding MIP prepared by FRP. The RAFT polymers were then tested in the chromatographic mode due to their ability to resolve the racemate D,L-PheNHPh. An optimum RAFT agent level during polymerization was found to result in a markedly enhanced selectivity, column efficiency and resolution accompanied by a considerably higher sample loading capacity when compared to polymers prepared in the absence of the RAFT agent

Synergistic Catalysis by “Polymeric Microzymes and Inorganic Nanozymes” : The 1+1>2 Effect for Intramolecular Cyclization of Peptides

In this work, we developed an efficient “molecularly imprinted polymer microzymes and inorganic magnetic nanozymes” synergistic catalysis strategy for the formation of disulfide bonds in peptides. The polymeric microzymes showed excellent selectivity toward the template peptide as well as the main reactant (linear peptide), and the Fe3O4 magnetic nanoparticle (MNP) nanozymes inhibited the intermolecular reaction during the formation of disulfide bonds in peptides. As a result, the integration of the two different artificial enzymes in one process facilitates the intramolecular cyclization in high product yields (59.3%) with excellent selectivity. Mechanism study indicates the synergistic effect was occurred by using a “reversed solid phase synthesis” strategy with an enhanced shift of reaction balance to product generation. We believe the synergistic catalysis by “polymeric microzymes and inorganic nanozymes” presented in the present work may open new opportunities in creation of multifunctional enzyme mimics for sensing, imaging, and drug delivery

Synergistic Catalysis by “Polymeric Microzymes and Inorganic Nanozymes”: The 1+1>2 Effect for Intramolecular Cyclization of Peptides

In this work, we developed an efficient “molecularly imprinted polymer microzymes and inorganic magnetic nanozymes” synergistic catalysis strategy for the formation of disulfide bonds in peptides. The polymeric microzymes showed excellent selectivity toward the template peptide as well as the main reactant (linear peptide), and the Fe3O4 magnetic nanoparticle (MNP) nanozymes inhibited the intermolecular reaction during the formation of disulfide bonds in peptides. As a result, the integration of the two different artificial enzymes in one process facilitates the intramolecular cyclization in high product yields (59.3%) with excellent selectivity. Mechanism study indicates the synergistic effect was occurred by using a “reversed solid phase synthesis” strategy with an enhanced shift of reaction balance to product generation. We believe the synergistic catalysis by “polymeric microzymes and inorganic nanozymes” presented in the present work may open new opportunities in creation of multifunctional enzyme mimics for sensing, imaging, and drug delivery

Productive encounter : molecularly imprinted nanoparticles prepared using magnetic templates

Synthesis of core-shell nanoparticles by surface initiated reversible addition fragmentation chain transfer polymerization in presence of a chiral template conjugated to magnetic nanoparticles is reported. The approach leads to imprinted nanoparticles featuring enantioselectivity and enhanced affinity compared to nanoparticles prepared using free template

Combinatorial design of a sialic acid imprinted binding site exploring a dual ion receptor approach

Aberrant sialic acid expression is one of the key indicators of pathological processes. This acidic saccharide is overexpressed in tumor cells and is a potent biomarker. Development of specific capture tools for various sialylated targets is an important step for early cancer diagnosis. However, sialic acid recognition by synthetic hosts is often complicated due to the competition for the anion binding by their counterions, such as Na+ and K+. Here we report on the design of a sialic acid receptor via simultaneous recognition of both the anion and cation of the target analyte. The polymeric receptor was produced using neutral (thio)urea and crown ether based monomers for simultaneous complexation of sialic acid's carboxylate group and its countercation. Thiourea and urea based functional monomers were tested both in solution by 1H NMR titration and in a polymer matrix system for their ability to complex the sodium salt of sialic acid alone and in the presence of crown ether. Combination of both orthogonally acting monomers resulted in higher affinities for the template in organic solvent media. The imprinted polymers displayed enhanced sialic acid recognition driven to a significant extent by the addition of the macrocyclic cation host. The effect of various counterions and solvent systems on the binding affinities is reported. Binding of K+, Na+ and NH4+ salts of sialic acid exceeded the uptake of bulky lipophilic salts. Polymers imprinted with sialic or glucuronic acids displayed a preference for their corresponding templates and showed a promising enrichment of sialylated peptides from the tryptic digest of glycoprotein bovine fetuin