Eugenia:towards disciplined and automated development of GMF-based graphical model editors

EMF and GMF are powerful frameworks for implementing tool support for modelling languages in Eclipse. However, with power comes complexity, implementing a graphical editor for a modelling language using EMF and GMF requires developers to handcraft and maintain several detailed interconnected models through a loosely guided, labour-intensive, and error-prone process. We demonstrate how the application of metamodel annotation and model transformation techniques can help to manage the complexity of GMF and EMF and deliver significant productivity, quality, and maintainability benefits. We present Eugenia, an open-source tool that implements the proposed approach, illustrate its functionality with an example, evaluate it through an empirical study, and report on the community’s response to the tool

Axonopathy in the central nervous system is the hallmark of mice with a novel intragenic null mutation of dystonin.

Dystonia musculorum is a neurodegenerative disorder caused by a mutation in the dystonin gene. It has been described in mice and humans where it is called hereditary sensory autonomic neuropathy. Mutated mice show severe movement disorders and die at the age of 3-4 weeks. This study describes the discovery and molecular, clinical, as well as pathological characterization of a new spontaneously occurring mutation in the dystonin gene in C57BL/6N mice. The mutation represents a 40-kb intragenic deletion allele of the dystonin gene on chromosome 1 with exactly defined deletion borders. It was demonstrated by Western blot, mass spectrometry, and immunohistology that mice with a homozygous mutation were entirely devoid of the dystonin protein. Pathomorphological lesions were restricted to the brain stem and spinal cord and consisted of swollen, argyrophilic axons and dilated myelin sheaths in the white matter and, less frequently, total chromatolysis of neurons in the gray matter. Axonal damage was detected by amyloid precursor protein and nonphosphorylated neurofilament immunohistology. Axonopathy in the central nervous system (CNS) represents the hallmark of this disease. Mice with the dystonin mutation also showed suppurative inflammation in the respiratory tract, presumably due to brain stem lesion-associated food aspiration, whereas skeletal muscles showed no pathomorphological changes. This study describes a novel mutation in the dystonin gene in mice leading to axonopathy in the CNS. In further studies, this model may provide new insights into the pathogenesis of neurodegenerative diseases and may elucidate the complex interactions of dystonin with various other cellular proteins especially in the CNS

Influencia de la variabilidad en la virulencia de diferentes aislados de Toxoplasma gondii sobre las lesiones de encéfalos fetales ovinos

Trabajo presentado a la: XXXII Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV). 1 octubre. Congreso virtual.Peer reviewe

Chance of aneurysm in patients suspected of SAH who have a ‘negative’ CT scan but a ‘positive’ lumbar puncture

In patients with sudden severe headache and a negative computed tomography (CT) scan, a lumbar puncture (LP) is performed to rule in or out a subarachnoid haemorrhage (SAH), but this procedure is under debate. In a hospital-based series of 30 patients with sudden headache, a negative CT scan but a positive LP (defined as detection of bilirubin >0.05 at wavelength 458 nm), we studied the chance of harbouring an aneurysm and the clinical outcome. Aneurysms were found in none of both patients who presented within 3 days, in 8 of the 18 (44%) who presented within 4–7 days and in 5 of the 10 (50%) who presented within 8–14 days. Of the 13 patients with an aneurysm, 3 (23%) had poor outcome. In patients who present late after sudden headache, the yield in terms of aneurysms is high in those who have a positive lumbar puncture. In patients with an aneurysm as cause of the positive lumbar puncture, outcome is in the same range as in SAH patients admitted in good clinical condition

Influenza A (H10N7) virus causes respiratory tract disease in harbor seals and ferrets

Avian influenza viruses sporadically cross the species barrier to mammals, including humans, in which they may cause epidemic disease. Recently such an epidemic occurred due to the emergence of avian influenza virus of the subtype H10N7 (Seal/H10N7) in harbor seals (Phoca vitulina). This epidemic caused high mortality in seals along the north-west coast of Europe and represented a potential risk for human health. To characterize the spectrum of lesions and to identify the target cells and viral distribution, findings in 16 harbor seals spontaneously infected with Seal/H10N7 are described. The seals had respiratory tract inflammation extending from the nasal cavity to bronchi associated with intralesional virus antigen in respiratory epithelial cells. Virus infection was restricted to the respiratory tract. The fatal outcome of the viral infection in seals was most likely caused by secondary bacterial infections. To investigate the pathogenic potential of H10N7 infection for humans, we inoculated the seal virus intratracheally into six ferrets and performed pathological and virological analyses at 3 and 7 days post inoculation. These experimentally inoculated ferrets displayed mild clinical signs, virus excretion from the pharynx and respiratory tract inflammation extending from bronchi to alveoli that was associated with virus antigen expression exclusively in the respiratory epithelium. Virus was isolated only from the respiratory tract. In conclusion, Seal/H10N7 infection in naturally infected harbor seals and experimentally infected ferrets shows that respiratory epithelial cells are the permissive cells for viral replication. Fatal outcome in seals was caused by secondary bacterial pneumonia similar to that in fatal human cases during influenza pandemics. Productive infection of ferrets indicates that seal/H10N7 may possess a zoonotic potential. This outbreak of LPAI from wild birds to seals demonstrates the risk of such occasions for mammals and thus humans

Soil compaction and stress propagation after different wheeling intensities on a silt soil in South-East Norway

The objective of this study was to evaluate the effect of wheeling with two different wheel loads (1.7 and 2.8 Mg) and contrasting wheeling intensities (1x and 10x) on the bearing capacity of a Stagnosol derived from silty alluvial deposits. Soil strength was assessed by laboratory measurements of the precompression stress in topsoil (20 cm) and subsoil (40 and 60 cm) samples. Stress propagation, as well as elastic and plastic deformation during wheeling were measured in the field with combined stress state (SST) and displacement transducers (DTS). We also present results from soil physical analyses (bulk density, air capacity, saturated hydraulic conductivity) and barley yields from the first two years after the compaction. Although the wheel loads used were comparatively small, typical for the machinery used in Norway, the results show that both increased wheel load and wheeling intensity had negative effects on soil physical parameters especially in the topsoil but with similar tendencies also in the subsoil. Stress propagation was detected down to 60 cm depth (SST). The first wheeling was most harmful, but all wheelings led to accumulative plastic soil deformation (DTS). Under the workable conditions in this trial, increased wheeling with a small machine was more harmful to soil structure than a single wheeling with a heavier machine. However, the yields in the first two years after the compaction did not show any negative effect of the compaction.acceptedVersio

Avian polyomavirus infection of a fledgling budgerigar (Melopsittacus undulatus) and differential diagnoses of viral inclusions in psittacine birds - case report and mini-review = Aviäre Polyomavirusinfektion bei einem jungen Wellensittich (Melopsittacus undulatus) und Differenzialdiagnostik viraler Einschlusskörperchen in Psittaciden - Fallbericht und Mini- Review

A two-week-old budgerigar (Melopsittacus undulatus) of an outdoor aviary diedsuddenly and was submitted for determination the cause of illness and death.Macroscopically, the sparsely feathered animal was in a poor body condition.Histopathological examination revealed in various mesenchymal and epithelialtissues, numerous up to 15 amp;#956;m in diameter large intranuclear, amphophilic tobasophilic inclusion bodies with a clearing of the centre. Additionally, a featherdysplasia and retention hyperkeratosis of feather follicles was found. Ultrastructurally,viral particles of approximately 35 nm in diameter were detected in thefeather follicle epithelium. A PCR for Avian Polyomavirus on fresh skin sampleswas negative whereas on formalin-fixed kidney samples with a high amount ofviral inclusion bodies yielded a positive result. In addition, viral inclusion bodydiseases, like Avian Poxvirus, Psittacine Beak and Feather disease virus, AvianAdenovirus, Psittacine Herpesvirus and papillomavirus of psittacines are summarizedand compared in the present article