Prospectus, March 8, 2000

https://spark.parkland.edu/prospectus_2000/1008/thumbnail.jp

Prospectus, February 23, 2000

https://spark.parkland.edu/prospectus_2000/1006/thumbnail.jp

Prospectus, November 10, 2010

CAMPUS TECH TO TACKLE BANDWIDTH ISSUE; Snow Day Woes: What to do when mother nature forbids going out; Chuck Shepherd\u27s News of the Weird; Human Pandora Pt. 3: Arcade Fire; World of Science lectures peak the interest of many; Lenville Stelle: A Rare Find at Parkland College; Greyhounds: Racing to the grave; No free rides\u27 for athletes; Do you mind if the FBI tracks your online habits?; Healthy Mind, Healthy Body Image: How a positive view of your body can lift your self-confidence; Virtual Embodiment: Tom Boellstorff discusses placing the virtual body; New from Cee Lo Green and Kid Cudi; Prepare to Access Success ; The Beginning of the Endhttps://spark.parkland.edu/prospectus_2010/1029/thumbnail.jp

Global diversity of the Brachypodium species complex as a resource for genome-wide association studies demonstrated for agronomic traits in response to climate

The development of model systems requires a detailed assessment of standing genetic variation across natural populations. The Brachypodium species complex has been promoted as a plant model for grass genomics with translation to small grain and biomass crops. To capture the genetic diversity within this species complex, thousands of Brachypodium accessions from around the globe were collected and genotyped by sequencing. Overall, 1897 samples were classified into two diploid or allopolyploid species, and then further grouped into distinct inbred genotypes. A core set of diverse B. distachyon diploid lines was selected for whole genome sequencing and high resolution phenotyping. Genome-wide association studies across simulated seasonal environments was used to identify candidate genes and pathways tied to key life history and agronomic traits under current and future climatic conditions. A total of 8, 22, and 47 QTL were identified for flowering time, early vigor, and energy traits, respectively. The results highlight the genomic structure of the Brachypodium species complex, and the diploid lines provided a resource that allows complex trait dissection within this grass model species.We are grateful for funding and support from the Australian Research Council Centre of Excellence in Plant Energy Biology (CE140100008). Australian Plant Phenomics Facility is supported under the National Collaborative Research Infrastructure Strategy of the Australian Government. The research was undertaken with the assistance of resources from the National Computational Infrastructure (NCI), which is supported by the Australian Government

Observing with the infrared array camera (IRAC) on the Spitzer Space Telescope

We describe the astronomical observation template (AOT) for the Infrared Array Camera (IRAC) on the Spitzer Space Telescope (formerly SIRTF, hereafter Spitzer). Commissioning of the AOTs was carried out in the first three months of the Spitzer mission. Strategies for observing fixed and moving targets are described, along with the performance of the AOT in flight. We also outline the operation of the IRAC data reduction pipeline at the Spitzer Science Center (SSC) and describe residual effects in the data due to electronic and optical anomalies in the instrument

Observing with the infrared array camera (IRAC) on the Spitzer Space Telescope

We describe the astronomical observation template (AOT) for the Infrared Array Camera (IRAC) on the Spitzer Space Telescope (formerly SIRTF, hereafter Spitzer). Commissioning of the AOTs was carried out in the first three months of the Spitzer mission. Strategies for observing fixed and moving targets are described, along with the performance of the AOT in flight. We also outline the operation of the IRAC data reduction pipeline at the Spitzer Science Center (SSC) and describe residual effects in the data due to electronic and optical anomalies in the instrument

Nutrient-Regulated Antisense and Intragenic RNAs Modulate a Signal Transduction Pathway in Yeast

The budding yeast Saccharomyces cerevisiae alters its gene expression profile in response to a change in nutrient availability. The PHO system is a well-studied case in the transcriptional regulation responding to nutritional changes in which a set of genes (PHO genes) is expressed to activate inorganic phosphate (Pi) metabolism for adaptation to Pi starvation. Pi starvation triggers an inhibition of Pho85 kinase, leading to migration of unphosphorylated Pho4 transcriptional activator into the nucleus and enabling expression of PHO genes. When Pi is sufficient, the Pho85 kinase phosphorylates Pho4, thereby excluding it from the nucleus and resulting in repression (i.e., lack of transcription) of PHO genes. The Pho85 kinase has a role in various cellular functions other than regulation of the PHO system in that Pho85 monitors whether environmental conditions are adequate for cell growth and represses inadequate (untimely) responses in these cellular processes. In contrast, Pho4 appears to activate some genes involved in stress response and is required for G1 arrest caused by DNA damage. These facts suggest the antagonistic function of these two players on a more general scale when yeast cells must cope with stress conditions. To explore general involvement of Pho4 in stress response, we tried to identify Pho4-dependent genes by a genome-wide mapping of Pho4 and Rpo21 binding (Rpo21 being the largest subunit of RNA polymerase II) using a yeast tiling array. In the course of this study, we found Pi- and Pho4-regulated intragenic and antisense RNAs that could modulate the Pi signal transduction pathway. Low-Pi signal is transmitted via certain inositol polyphosphate (IP) species (IP7) that are synthesized by Vip1 IP6 kinase. We have shown that Pho4 activates the transcription of antisense and intragenic RNAs in the KCS1 locus to down-regulate the Kcs1 activity, another IP6 kinase, by producing truncated Kcs1 protein via hybrid formation with the KCS1 mRNA and translation of the intragenic RNA, thereby enabling Vip1 to utilize more IP6 to synthesize IP7 functioning in low-Pi signaling. Because Kcs1 also can phosphorylate these IP7 species to synthesize IP8, reduction in Kcs1 activity can ensure accumulation of the IP7 species, leading to further stimulation of low-Pi signaling (i.e., forming a positive feedback loop). We also report that genes apparently not involved in the PHO system are regulated by Pho4 either dependent upon or independent of the Pi conditions, and many of the latter genes are involved in stress response. In S. cerevisiae, a large-scale cDNA analysis and mapping of RNA polymerase II binding using a high-resolution tiling array have identified a large number of antisense RNA species whose functions are yet to be clarified. Here we have shown that nutrient-regulated antisense and intragenic RNAs as well as direct regulation of structural gene transcription function in the response to nutrient availability. Our findings also imply that Pho4 is present in the nucleus even under high-Pi conditions to activate or repress transcription, which challenges our current understanding of Pho4 regulation