System and method for monitoring cellular activity

A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands

Metformin as a Therapeutic Target in Endometrial Cancers.

Endometrial cancer is the most common gynecologic malignancy in developed countries. Its increasing incidence is thought to be related in part to the rise of metabolic syndrome, which has been shown to be a risk factor for the development of hyperestrogenic and hyperinsulinemic states. This has consequently lead to an increase in other hormone-responsive cancers as well e.g., breast and ovarian cancer. The correlation between obesity, hyperglycemia, and endometrial cancer has highlighted the important role of metabolism in cancer establishment and persistence. Tumor-mediated reprogramming of the microenvironment and macroenvironment can range from induction of cytokines and growth factors to stimulation of surrounding stromal cells to produce energy-rich catabolites, fueling the growth, and survival of cancer cells. Such mechanisms raise the prospect of the metabolic microenvironment itself as a viable target for treatment of malignancies. Metformin is a biguanide drug that is a first-line treatment for type 2 diabetes that has beneficial effects on various markers of the metabolic syndrome. Many studies suggest that metformin shows potential as an adjuvant treatment for uterine and other cancers. Here, we review the evidence for metformin as a treatment for cancers of the endometrium. We discuss the available clinical data and the molecular mechanisms by which it may exert its effects, with a focus on how it may alter the tumor microenvironment. The pleiotropic effects of metformin on cellular energy production and usage as well as intercellular and hormone-based interactions make it a promising candidate for reprogramming of the cancer ecosystem. This, along with other treatments aimed at targeting tumor metabolic pathways, may lead to novel treatment strategies for endometrial cancer

Development of a carbon neutral process for producing renewable charcoal

This thesis investigates the process conditions, charcoal composition and biomass sources for process development of BBQ charcoal briquette production. This aim has been achieved by three areas of investigation: 1. Experimental pyrolysis at lab and pilot scale, with the aim of determining optimal process conditions and if addition of low cost catalyst material is beneficial to slow pyrolysis. 2. BBQ charcoal combustion and modelling of the fire temperature history to determine the pyrolysis conditions and allowable concentrations of non-charcoal additive important to the final charcoal briquette. 3. Techno-economic and carbon analysis (TEA) of the various options for by-product utilisations and determination of important process conditions. The novel findings from the experimental and modelling studies add to the literature on catalytic slow pyrolysis and lump char combustion, whilst applying these concepts to present optimal process flows and conditions for BBQ charcoal production. 1. The addition of low cost acidic and basic catalyst such as bentonite clay or dolomite to the slow pyrolysis of biomass can significantly increase the charcoal yield, and significantly improve the process economics. This also improves the quality of by-product liquid, high in low molecular weight phenolic compounds. 2. Lump charcoal combustion in a grate burner can be simulated by applying the analogy of a two first order chemical reactions in series model to the temperature profile of a simple combustion experiment. The model only requires proximate analysis of the charcoal briquette, and is valid up to briquette mixtures containing 30% inert or raw biomass additive. 3. Temperatures, around 450°C, are required. Lower temperature increases charcoal yield and therefore process economics and this is not too low to produce poor quality charcoal. 4. Processes either A) utilising the gas and liquid by-products in a combined heat and power engine or B) recovering the chemical stream as phenolic oils and using the gas stream to produce heat are the most economic and carbon efficient outcomes. 5. Addition of clay minerals are key to the economic success of both processes by increasing charcoal yield and producing better quality oil high in low molecular weight phenolic compounds

Shrinking binary and planetary orbits by Kozai cycles with tidal friction

At least two arguments suggest that the orbits of a large fraction of binary stars and extrasolar planets shrank by 1-2 orders of magnitude after formation: (i) the physical radius of a star shrinks by a large factor from birth to the main sequence, yet many main-sequence stars have companions orbiting only a few stellar radii away, and (ii) in current theories of planet formation, the region within ~0.1 AU of a protostar is too hot and rarefied for a Jupiter-mass planet to form, yet many "hot Jupiters" are observed at such distances. We investigate orbital shrinkage by the combined effects of secular perturbations from a distant companion star (Kozai oscillations) and tidal friction. We integrate the relevant equations of motion to predict the distribution of orbital elements produced by this process. Binary stars with orbital periods of 0.1 to 10 days, with a median of ~2 d, are produced from binaries with much longer periods (10 d to 10^5 d), consistent with observations indicating that most or all short-period binaries have distant companions (tertiaries). We also make two new testable predictions: (1) For periods between 3 and 10 d, the distribution of the mutual inclination between the inner binary and the tertiary orbit should peak strongly near 40 deg and 140 deg. (2) Extrasolar planets whose host stars have a distant binary companion may also undergo this process, in which case the orbit of the resulting hot Jupiter will typically be misaligned with the equator of its host star.Comment: Submitted to ApJ; 18 pages, 10 figure

Transplantation of Ciliary Neurotrophic Factor-Expressing Adult Oligodendrocyte Precursor Cells Promotes Remyelination and Functional Recovery after SpinalCord Injury

Demyelination contributes to the dysfunction after traumatic spinal cord injury (SCI). We explored whether the combination of neurotrophic factors and transplantation of adult rat spinal cord oligodendrocyte precursor cells (OPCs) could enhance remyelination and functional recovery after SCI. Ciliary neurotrophic factor (CNTF) was the most effective neurotrophic factor to promote oligodendrocyte (OL) differentiation and survival of OPCs in vitro. OPCs were infected with retroviruses expressing enhanced green fluorescent protein (EGFP) or CNTF and transplanted into the contused adult thoracic spinal cord 9 d after injury. Seven weeks after transplantation, the grafted OPCs survived and integrated into the injured spinal cord. The survival of grafted CNTF-OPCs increased fourfold compared with EGFP-OPCs. The grafted OPCs differentiated into adenomatus polyposis coli (APC+) OLs, and CNTF significantly increased the percentage of APC+ OLs from grafted OPCs. Immunofluorescent and immunoelectron microscopic analyses showed that the grafted OPCs formed central myelin sheaths around the axons in the injured spinal cord. The number of OL-remyelinated axons in ventrolateral funiculus (VLF) or lateral funiculus (LF) at the injured epicenter was significantly increased in animals that received CNTF-OPC grafts compared with all other groups. Importantly, 75% of rats receiving CNTF-OPC grafts recovered transcranial magnetic motor-evoked potential and magnetic interenlargement reflex responses, indicating that conduction through the demyelinated axons in VLF or LF, respectively, was partially restored. More importantly, recovery of hindlimb locomotor function was significantly enhanced in animals receiving grafts of CNTF-OPCs. Thus, combined treatment with OPC grafts expressing CNTF can enhance remyelination and facilitate functional recovery after traumatic SCI

Reassortant Pandemic (H1N1) 2009 Virus in Pigs, United Kingdom

Surveillance for influenza virus in pigs in the United Kingdom during spring 2010 detected a novel reassortant influenza virus. This virus had genes encoding internal proteins from pandemic (H1N1) 2009 virus and hemagglutinin and neuraminidase genes from swine influenza virus (H1N2). Our results demonstrate processes contributing to influenza virus heterogeneity