Otvaranje zvukovnih arhiva Europe: projekt Europeana Sounds

Europeana Sounds is a project co-funded by the European Commission within the ICT Policy Support Programme as part of the Competitiveness and Innovation Framework Programme. The project aimed to enhance the total amount of the sound contents available through Europeana. Currently, more than 600,000 audio items and more than 300,000 audio-related contents have been aggregated thanks to the activities carried out by Europeana Sounds. The project has achieved other important objectives such as the enrichment of the metadata related to the digital objects, the realization of a set of Policy Recommendations, the launch of the first thematic channel of Europeana entirely devoted to the music named Europeana Music and a Radio that makes 200,000 music tracks available online. Europeana Sounds will continue to pursuit its objectives through the Task force established within the International Association of Sound and Audiovisual Archives (IASA).Slogan projekta Europeana Sounds kaže »Zvukovno nasljeđe Europe pod vašim prstima « i to je, u suštini, glavni cilj Projekta. Ovaj članak daje pregled Europeana Sounds projekta koji je započet u veljači 2014, a završen u siječnju 2017. godine. Projekt čini dostupnim sadržaje sakupljene putem Europeane koja nudi pristup do više od 53,870.000 izvora, uključujući knjige, videosnimke, slike, zvukove, umjetnička djela i još mnogo više iz europskog kulturnog nasljeđa. U prvome dijelu članka objašnjavaju se ciljevi Europeana Sounds, a to su: povećati ukupnu količinu zvučnih izvora koji su dostupni putem portala Europeana, obogatiti metapodatke vezane uz te izvore, kreirati specifičan kanal posvećen zvukovima i ostalim srodnim sadržajima, istražiti u svrhu rješavanja ograničenja domene vezane uz zvukovno nasljeđe, potaknuti kreativno korištenje navedenih resursa te kreirati mrežu interesenata i stručnjaka na polju zvuka. Unutar projekta formirano je nekoliko grupa koje su provele radnje vezane uz konačne ciljeve projekta: od sakupljanja sadržaja do tehničke infrastrukture koja omogućava objavljivanje na Europeani, od obogaćivanja metapodataka vezanih za izvore i crowdsourcinga do pitanja autorskih prava, od realizacije tematskog kanala do složenih aktivnosti vođenja projekta. Za vrijeme projekta svi postignuti ciljevi promovirani su kroz širok spektar aktivnosti, uključujući dva međunarodna skupa, sudjelovanjem na događanjima, dinamičnim blogom s više objava koje su vezane za zvukovno nasljeđe te stranicama na društvenim mrežama. Zaključno, trud uložen u pokretanje tematskoga kanala posvećenoga glazbi. Projekt, koji je u rasponu od tri godine ostvario razne ciljeve, nastavit će svoje aktivnosti zahvaljujući radnoj skupini formiranoj unutar International Association of Sound and Audiovisual Archives (IASA)

Bootstrapping the Chiral Anomaly at Large NcN_c

The bootstrap approach (demanding consistency conditions to scattering amplitudes) has shown to be quite powerful to tightly constrain gauge theories at large $N_c$. We extend previous analysis to scattering amplitudes involving pions and external gauge bosons. These amplitudes allow us to access the chiral anomaly and connect low-energy physical quantities to UV properties of the theory. In particular, we are able to obtain an analytic bound on the chiral anomaly coefficient as a function of the pion dipole polarizabilities. This bound can be useful for holographic models whose dual UV completions are not known, and provide a consistency condition to lattice simulations.Comment: 20 pages, 1 figure. v2: bound on the anomaly generalised and typos correcte

IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems

A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances

Building response to tunnelling

AbstractUnderstanding how buildings respond to tunnelling-induced ground movements is an area of great importance for urban tunnelling projects, particularly for risk management. In this paper, observations of building response to tunnelling, from both centrifuge modelling and a field study in Bologna, are used to identify mechanisms governing the soil–structure interaction. Centrifuge modelling was carried out on an 8-m-diameter beam centrifuge at Cambridge University, with buildings being modelled as highly simplified elastic and inelastic beams of varying stiffness and geometry. The Bologna case study presents the response of two different buildings to the construction of a sprayed concrete lining (SCL) tunnel, 12m in diameter, with jet grouting and face reinforcement.In both studies, a comparison of the building settlement and horizontal displacement profiles, with the greenfield ground movements, enables the soil structure interaction to be quantified. Encouraging agreement between the modification to the greenfield settlement profile, displayed by the buildings, and estimates made from existing predictive tools is observed. Similarly, both studies indicate that the horizontal strains, induced in the buildings, are typically at least an order of magnitude smaller than the greenfield values. This is consistent with observations in the literature. The potential modification to the settlement distortions is shown to have significant implications on the estimated level of damage. Potential issues for infrastructures connected to buildings, arising from the embedment of rigid buildings into the soil, are also highlighted

The classical Lenz vector and the two-dimensional quantum harmonic oscillator

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Considerazioni sulla rilevanza qualificatoria della certificazione dei contratti di lavoro = Considerations on the qualifying relevance of the certification of employment contracts. WP C.S.D.L.E. "Massimo D'Antona" .IT - 24/2004

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Development and Characterization of an Enzymatic Method for the Rapid Determination of Gamma Hydroxybutyric Acid

Gamma hydroxybutyric acid (GHB) is a regulated therapeutic drug, which naturally occurs in mammalian brain tissues as an intermediate of the GABA (gamma aminobutyric acid) neurotransmitter metabolism. The increasing misuse of GHB as a narcotic or abusing drug in recent years calls for the development of a simple and rapid screening method as an alternative to the currently available, technically demanding diagnostic methods. We have developed a rapid enzymatic assay based on the GHB dehydrogenase of Ralstonia eutropha. The enzyme is expressed as a recombinant protein in Escherichia coli and characterized in terms of reaction mechanism and kinetic parameters for the catalysis of conversion of GHB into succinic semialdehyde (SSA). The concomitant NADH production enables spectrophotometric monitoring of the reaction and the quantification of GHB in physiological fluids depending on initial velocities. We have tested a panel of twelve serum and urine samples containing GHB concentrations from 0.0 to 2.1 mmol/L. GHB dehydrogenase activity obeys a non classical bi bi ping pong mechanism exhibiting substrate inhibition by NAD+. With an optimal NAD+ concentration of 3.7 mmol/L in the reaction, the enzyme yields a KM of 1.0 mmol/L for GHB and a Vmax of 3.37 mmol/min/mg. The assay shows a linear standard curve from 0.1 to at least 1 mmol/L of GHB. Spiking experiments result in mean recoveries of 92% for urine and 114% for serum, respectively. The comparison to an ion chromatographic reference method exhibits a mean difference of 10% divergence from the target values in urine and 9% in serum, respectively

Antimalarials with Benzothiophene Moieties as Aminoquinoline Partners

Malaria is a severe and life-threatening disease caused by Plasmodium parasites that are spread to humans through bites of infected Anopheles mosquitoes. Here, we report on the efficacy of aminoquinolines coupled to benzothiophene and thiophene rings in inhibiting Plasmodium falciparum parasite growth. Synthesized compounds were evaluated for their antimalarial activity and toxicity, in vitro and in mice. Benzothiophenes presented in this paper showed improved activities against a chloroquine susceptible (CQS) strain, with potencies of IC50 = 6 nM, and cured 5/5 Plasmodium berghei infected mice when dosed orally at 160 mg/kg/day x 3 days. In the benzothiophene series, the examined antiplasmodials were more active against the CQS strain D6, than against strains chloroquine resistant (CQR) W2 and multidrug-resistant (MDR) TM91C235. For the thiophene series, a very interesting feature was revealed: hypersensitivity to the CQR strains, resistance index (RI) of lt 1. This is in sharp contrast to chloroquine, indicating that further development of the series would provide us with more potent antimalarials against CQR strains

Supplementary data for article : Konstantinović, J. M.; Selaković, M.; Srbljanovic, J.; Djurkovic-Djakovic, O.; Bogojević, K.; Sciotti, R.; Šolaja, B. A. Antimalarials with Benzothiophene Moieties as Aminoquinoline Partners. Molecules 2017, 22 (3). https://doi.org/10.3390/molecules22030343

Supplementary material for: [https://doi.org/10.3390/molecules22030343]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2441

Supplementary data for article: Opsenica, I.; Tot, M.; Gomba, L.; Nuss, J. E.; Sciotti, R. J.; Bavari, S.; Burnett, J. C.; Šolaja, B. A. 4-Amino-7-Chloroquinolines: Probing Ligand Efficiency Provides Botulinum Neurotoxin Serotype A Light Chain Inhibitors with Significant Antiprotozoal Activity. Journal of Medicinal Chemistry 2013, 56 (14), 5860–5871. https://doi.org/10.1021/jm4006077

Supporting information for: [https://doi.org/10.1021/jm4006077]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1381