OEIGWG has come in from the cold: Will the EU do the same? Position paper on the zero draft of a binding treaty presented by the open-ended intergovernmental working group on transnational companies and other business enterprises

In July, the Open Ended Intergovernmental Working Group on Transnational Corporations and Other Business Enterprises, chaired by Ecuador, published the zero draft of the text of a treaty on business and human rights. This draft is the basis for negotiations in Geneva from 15-19 October 2018. The text represents a good first basis for further negotations among UN member states, and it goes some way toward closing protection gaps, especially in global supply chains. Helpfully, it builds on the UN Guiding Principles for Business and Human Rights and focuses on the most urgent areas in this field: the prevention of human rights abuses and access to effective remedy for victims. Rather than elevating corporations to direct subjects of international law, it bolsters the existing architecture of human rights protection: the state duty to protect, enforced by civil and criminal liability. These positive developments compared with earlier documents from the treaty process should be recognized by the UN member states and especially by the European Union. Further rounds of negotiations must now achieve the necessary precision, ironing out of contradicitons, and further development of the text

Oral health and oral health behaviour of adolescents with mild or borderline intellectual disabilities compared with a national representative sample of 17-year-olds in the Netherlands

Background People with intellectual disabilities have worse health outcomes compared to their peers without. However, regarding oral health parameters, recent systematic reviews reported conflicting evidence. The aim was to assess whether adolescents with MBID differ from their peers in oral health and oral health behaviour. Methods Ninety seven adolescents with MBID participated in this comparative study. Outcomes were compared to data of 17-year-old Dutch adolescents (n = 581) from a national epidemiological study on oral health and oral health behaviour. Results Adolescents with MBID showed worse oral health outcomes and poorer oral hygiene than their peers from the general population. Furthermore, they visit the dentist less regularly, brush less frequently, eat main-dishes less frequently and have higher levels of dental anxiety. Conclusion Adolescents with MBID have poorer oral health and show worse oral health-promoting behaviours than their peers in the general population. Targeted interventions to reach this vulnerable group are necessary

Object-Oriented Echo Perception and Cortical Representation in Echolocating Bats

Echolocating bats can identify three-dimensional objects exclusively through the analysis of acoustic echoes of their ultrasonic emissions. However, objects of the same structure can differ in size, and the auditory system must achieve a size-invariant, normalized object representation for reliable object recognition. This study describes both the behavioral classification and the cortical neural representation of echoes of complex virtual objects that vary in object size. In a phantom-target playback experiment, it is shown that the bat Phyllostomus discolor spontaneously classifies most scaled versions of objects according to trained standards. This psychophysical performance is reflected in the electrophysiological responses of a population of cortical units that showed an object-size invariant response (14/109 units, 13%). These units respond preferentially to echoes from objects in which echo duration (encoding object depth) and echo amplitude (encoding object surface area) co-varies in a meaningful manner. These results indicate that at the level of the bat's auditory cortex, an object-oriented rather than a stimulus-parameter–oriented representation of echoes is achieved

Effect of Lipid Head Groups on Double-Layered Two-Dimensional Crystals Formed by Aquaporin-0

Aquaporin-0 (AQP0) is a lens-specific water channel that also forms membrane junctions. Reconstitution of AQP0 with dimyristoyl phosphatidylcholine (DMPC) and E. coli polar lipids (EPL) yielded well-ordered, double-layered two-dimensional (2D) crystals that allowed electron crystallographic structure determination of the AQP0-mediated membrane junction. The interacting tetramers in the two crystalline layers are exactly in register, resulting in crystals with p422 symmetry. The high-resolution density maps also allowed modeling of the annular lipids surrounding the tetramers. Comparison of the DMPC and EPL bilayers suggested that the lipid head groups do not play an important role in the interaction of annular lipids with AQP0. We now reconstituted AQP0 with the anionic lipid dimyristoyl phosphatidylglycerol (DMPG), which yielded a mixture of 2D crystals with different symmetries. The different crystal symmetries result from shifts between the two crystalline layers, suggesting that the negatively charged PG head group destabilizes the interaction between the extracellular AQP0 surfaces. Reconstitution of AQP0 with dimyristoyl phosphatidylserine (DMPS), another anionic lipid, yielded crystals that had the usual p422 symmetry, but the crystals showed a pH-dependent tendency to stack through their cytoplasmic surfaces. Finally, AQP0 failed to reconstitute into membranes that were composed of more than 40% dimyristoyl phosphatidic acid (DMPA). Hence, although DMPG, DMPS, and DMPA are all negatively charged lipids, they have very different effects on AQP0 2D crystals, illustrating the importance of the specific lipid head group chemistry beyond its mere charge

G048.66-0.29: physical state of an isolated site of massive star formation

We present continuum observations of the infrared dark cloud (IRDC) G48.66-0.22 (G48) obtained with Herschel, Spitzer, and APEX, in addition to several molecular line observations. The Herschel maps are used to derive temperature and column density maps of G48 using a model based on a modified blackbody. We find that G48 has a relatively simple structure and is relatively isolated; thus, this IRDC provides an excellent target to study the collapse and fragmentation of a filamentary structure in the absence of complicating factors such as strong external feedback. The derived temperature structure of G48 is clearly non-isothermal from cloud to core scale. The column density peaks are spatially coincident with the lowest temperatures (~17.5 K) in G48. A total cloud mass of ~390 M ⊙ is derived from the column density maps. By comparing the luminosity-to-mass ratio of 13 point sources detected in the Herschel/PACS bands to evolutionary models, we find that two cores are likely to evolve into high-mass stars (M sstarf >= 8 M ⊙). The derived mean projected separation of point sources is smaller than in other IRDCs but in good agreement with theoretical predications for cylindrical collapse. We detect several molecular species such as CO, HCO+, HCN, HNC, and N2H+. CO is depleted by a factor of ~3.5 compared to the expected interstellar abundance, from which we conclude that CO freezes out in the central region. Furthermore, the molecular clumps, associated with the submillimeter peaks in G48, appear to be gravitationally unbound or just pressure confined. The analysis of critical line masses in G48 shows that the entire filament is collapsing, overcoming any internal support

The Prevalence of Tooth Wear in the Dutch Adult Population

This study aimed to assess the prevalence of tooth wear in different age groups of the Dutch adult population and to determine this tooth wear distribution by gender, socioeconomic class, and type of teeth. Results were compared with the outcomes of a previous study in a comparable population. As part of a comprehensive investigation of the oral health of the general Dutch adult population in 2013, tooth wear was assessed among 1,125 subjects in the city of 's-Hertogenbosch. The data collected were subjected to stratified analysis by 5 age groups (25-34, 35-44, 45-54, 55-64, and 65-74 years), gender, socioeconomic class, and type of teeth. Tooth wear was assessed using a 5-point ordinal occlusal/incisal grading scale. The number of teeth affected was higher in older age groups. Men showed more tooth wear than women, and subjects with low socioeconomic status (low SES) showed on average higher scores than those with high SES. Tooth wear prevalence found in this study was higher in all age groups than in the previous study. The present study found prevalences of 13% for mild tooth wear and 80% for moderate tooth wear, leading to the conclusion that these are common conditions in the Dutch adult population. Severe tooth wear (prevalence 6%) may however be characterized as rare. A tendency was found for there to be more tooth wear in older age groups, in men as compared with women, in persons with lower SES, and in the present survey as compared with the previous one

The Earliest Phases of Star Formation (EPoS): A Herschel Key Program - The precursors to high-mass stars and clusters

(Abridged) We present an overview of the sample of high-mass star and cluster forming regions observed as part of the Earliest Phases of Star Formation (EPoS) Herschel Guaranteed Time Key Program. A sample of 45 infrared-dark clouds (IRDCs) were mapped at PACS 70, 100, and 160 micron and SPIRE 250, 350, and 500 micron. In this paper, we characterize a population of cores which appear in the PACS bands and place them into context with their host cloud and investigate their evolutionary stage. We construct spectral energy distributions (SEDs) of 496 cores which appear in all PACS bands, 34% of which lack counterparts at 24 micron. From single-temperature modified blackbody fits of the SEDs, we derive the temperature, luminosity, and mass of each core. These properties predominantly reflect the conditions in the cold, outer regions. Taking into account optical depth effects and performing simple radiative transfer models, we explore the origin of emission at PACS wavelengths. The core population has a median temperature of 20K and has masses and luminosities that span four to five orders of magnitude. Cores with a counterpart at 24 micron are warmer and bluer on average than cores without a 24 micron counterpart. We conclude that cores bright at 24 micron are on average more advanced in their evolution, where a central protostar(s) have heated the outer bulk of the core, than 24 micron-dark cores. The 24 micron emission itself can arise in instances where our line of sight aligns with an exposed part of the warm inner core. About 10% of the total cloud mass is found in a given cloud's core population. We uncover over 300 further candidate cores which are dark until 100 micron. These are candidate starless objects, and further observations will help us determine the nature of these very cold cores.Comment: Accepted for publication in A&A, 81 pages, 68 figures. For full resolution image gallery (Appendix B), see http://www.mpia.de/~ragan/epos.htm

Limited predictive value of FDG-PET for response assessment in the preoperative treatment of esophageal cancer : results of a prospective multi-center trial (SAKK 75/02)

BACKGROUND: Only responding patients benefit from preoperative therapy for locally advanced esophageal carcinoma. Early detection of non-responders may avoid futile treatment and delayed surgery. PATIENTS AND METHODS: In a multi-center phase ll trial, patients with resectable, locally advanced esophageal carcinoma were treated with 2 cycles of induction chemotherapy followed by chemoradiotherapy (CRT) and surgery. Positron emission tomography with 2[fluorine-18]fluoro-2-deoxy-d-glucose (FDG-PET) was performed at baseline and after induction chemotherapy. The metabolic response was correlated with tumor regression grade (TRG). A decrease in FDG tumor uptake of less than 40% was prospectively hypothesized as a predictor for histopathological non-response (TRG < 2) after CRT. RESULTS: 45 patients were included. The median decrease in FDG tumor uptake after chemotherapy correlated well with TRG after completion of CRT (p = 0.021). For an individual patient, less than 40% decrease in FDG tumor uptake after induction chemotherapy predicted histopathological non-response after completion of CRT, with a sensitivity of 68% and a specificity of 52% (positive predictive value 58%, negative predictive value 63%). CONCLUSIONS: Metabolic response correlated with histopathology after preoperative therapy. However, FDG-PET did not predict non-response after induction chemotherapy with sufficient clinical accuracy to justify withdrawal of subsequent CRT and selection of patients to proceed directly to surgery

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity

Vesicle-inducing protein in plastids 1 (VIPP1) is essential for the biogenesis and maintenance of thylakoid membranes, which transform light into life. However, it is unknown how VIPP1 performs its vital membrane-remodeling functions. Here, we use cryo-electron microscopy to determine structures of cyanobacterial VIPP1 rings, revealing how VIPP1 monomers flex and interweave to form basket-like assemblies of different symmetries. Three VIPP1 monomers together coordinate a non-canonical nucleotide binding pocket on one end of the ring. Inside the ring's lumen, amphipathic helices from each monomer align to form large hydrophobic columns, enabling VIPP1 to bind and curve membranes. In vivo mutations in these hydrophobic surfaces cause extreme thylakoid swelling under high light, indicating an essential role of VIPP1 lipid binding in resisting stress-induced damage. Using cryo-correlative light and electron microscopy (cryo-CLEM), we observe oligomeric VIPP1 coats encapsulating membrane tubules within the Chlamydomonas chloroplast. Our work provides a structural foundation for understanding how VIPP1 directs thylakoid biogenesis and maintenance