Biodegradation of Organophosphate Chemical Warfare Agents by Activated Sludge

Organophosphates (OPs) have been widely used as Chemical Warfare Agents (CWAs) as well as pesticides since World War II and still remain a threat to national security. While efforts have been taken at military installations and civilian communities to secure these chemicals and prevent their misuse, a determined adversary could still obtain and deploy them to injure, kill, or instill terror. The lethal properties of this group of compounds are primarily owed to their irreversible inhibition of the enzyme acetyl cholinesterase (AChE) and thus may alter the human nervous system or affect the hormonal balance of children in particular. In the event of a chemical incident, standard operating procedures dictate that contaminated personnel be decontaminated. Often times, decontamination is accomplished with water. Many communities plan for this decontamination water to be sent to the local municipal wastewater treatment plant. However, the fate of these compounds in a municipal wastewater treatment plant is largely unknown. If the compounds cannot be degraded, they will enter surface water bodies with plant effluent or waste sludge. This study investigated the fate of ethyl methylphosphonic acid (EMPA), a hydrolysis product of VX, in a single sludge laboratory-scale sequencing batch reactor (SBR) that simulated a municipal activated sludge wastewater treatment plant. The reactor was fed peptone and sodium acetate to simulate wastewater. Sorption kinetics, sorption equilibrium isotherm, and degradation batch experiments demonstrated that EMPA did not sorb to the biomass. Degradation results showed that approximately 28% of the initial concentration of 1 mg L-1 EMPA was degraded. In addition, the results suggest that the nitrifying bacteria may be responsible for the degradation via cometabolism. Therefore, CWA may pass through an activated sludge wastewater treatment plant completely unchanged

Mastermind Acts Downstream of Notch to Specify Neuronal Cell Fates in theDrosophilaCentral Nervous System

AbstractIn theDrosophilacentral nervous system, cellular diversity is generated through the asymmetric partitioning of cell fate determinants at cell division. Neural precursors (or neuroblasts) divide in a stem cell lineage to generate a series of ganglion mother cells, each of which divides once to produce a pair of postmitotic neurons or glial cells. An exception to this rule is the MP2 neuroblast, which divides only once to generate two neurons. We screened for genes expressed in the MP2 neuroblast and its progeny as a means of identifying the factors that specify cell fate in the MP2 lineage. We identified a P-element insertion line that expresses the reporter gene, tau-β-galactosidase, in the MP2 precursor and its progeny, the vMP2 and dMP2 neurons. The transposon disrupts the neurogenic gene,mastermind,but does not lead to neural hyperplasia. However, the vMP2 neuron is transformed into its sibling cell, dMP2. By contrast, expression of a dominant activated form of the Notch receptor in the MP2 lineage transforms dMP2 to vMP2. Notch signalling requires Mastermind, suggesting that Mastermind acts downstream of Notch to determine the vMP2 cell fate. We show that Mastermind plays a similar role in the neurons derived from ganglion mother cells 1-1a and 4-2a, where it specifies the pCC and RP2sib fates, respectively. This suggests that Notch signalling through Mastermind plays a wider role in specifying neuronal identity in theDrosophilacentral nervous system

Silicon-on ceramic process: Silicon sheet growth and device development for the large-area silicon sheet task of the low-cost solar array project

The technical feasibility of producing solar-cell-quality sheet silicon to meet the Department of Energy (DOE) 1986 overall price goal of $0.70/watt was investigated. With the silicon-on-ceramic (SOC) approach, a low-cost ceramic substrate is coated with large-grain polycrystalline silicon by unidirectional solidification of molten silicon. This effort was divided into several areas of investigation in order to most efficiently meet the goals of the program. These areas include: (1) dip-coating; (2) continuous coating designated SCIM-coating, and acronym for Silicon Coating by an Inverted Meniscus (SCIM); (3) material characterization; (4) cell fabrication and evaluation; and (5) theoretical analysis. Both coating approaches were successful in producing thin layers of large grain, solar-cell-quality silicon. The dip-coating approach was initially investigated and considerable effort was given to this technique. The SCIM technique was adopted because of its scale-up potential and its capability to produce more conventiently large areas of SOC

Silicon on ceramic process. Silicon sheet growth development for the large-area silicon sheet task of the low-cost silicon solar array project

The technical and economic feasibility of producing solar-cell-quality sheet silicon was investigated. The sheets were made by coating one surface of carbonized ceramic substrates with a thin layer of large-grain polycrystalline silicon from the melt. Significant progress was made in all areas of the program

A Sustainable Prototype for Renewable Energy: Optimized Prime-power Generator Solar Array Replacement

Remote locations such as disaster relief camps, isolated arctic communities, and military forward operating bases are disconnected from traditional power grids forcing them to rely on diesel generators with a total installed capacity of 10,000 MW worldwide. The generators require a constant resupply of fuel, resulting in increased operating costs, negative environmental impacts, and challenging fuel logistics. To enhance remote site sustainability, planners can develop stand-alone photovoltaic-battery systems to replace existing prime power generators. This paper presents the development of a novel cost-performance model capable of optimizing solar array and Li-ion battery storage size by generating tradeoffs between minimizing initial system cost and maximizing power reliability. A case study for the replacement of an 800 kW generator, the US Air Force’s standard for prime power at deployed locations, was analyzed to demonstrate the model and its capabilities. A MATLAB model, simulating one year of solar data, was used to generate an optimized solution to minimize initial cost while providing over 99% reliability. Replacing a single diesel generator would result in a savings of 1.9 million liters of fuel, eliminating 100 fuel tanker truck deliveries annually. The distinctive capabilities of this model enable designers to enhance environmental, economic, and operational sustainability of remote locations by creating energy self-sufficient sites, which can operate indefinitely without the need for resupply

Dip-coating process: Silicon sheet growth development for the large-area silicon sheet task of the low-cost silicon solar array project

The objective of this research program is to investigate the technical and economic feasibility of producing solar-cell-quality sheet silicon by coating one surface of carbonized ceramic substrates with a thin layer of large-grain polycrystalline silicon from the melt. The past quarter demonstrated significant progress in several areas. Seeded growth of silicon-on-ceramic (SOC) with an EFG ribbon seed was demonstrated. Different types of mullite were successfully coated with silicon. A new method of deriving minority carrier diffusion length, L sub n from spectral response measurements was evaluated. ECOMOD cost projections were found to be in good agreement with the interim SAMIS method proposed by JPL. On the less positive side, there was a decrease in cell performance which we believe to be due to an unidentified source of impurities

Silicon on Ceramic Process: Silicon Sheet Growth and Device Development for the Large-area Silicon Sheet and Cell Development Tasks of the Low-cost Solar Array Project

The technical and economic feasibility of producing solar cell quality sheet silicon was investigated. It was hoped this could be done by coating one surface of carbonized ceramic substrates with a thin layer of large-grain polycrystalline silicon from the melt. Work was directed towards the solution of unique cell processing/design problems encountered with the silicon-ceramic (SOC) material due to its intimate contact with the ceramic substrate. Significant progress was demonstrated in the following areas; (1) the continuous coater succeeded in producing small-area coatings exhibiting unidirectional solidification and substatial grain size; (2) dip coater succeeded in producing thick (more than 500 micron) dendritic layers at coating speeds of 0.2-0.3 cm/sec; and (3) a standard for producing total area SOC solar cells using slotted ceramic substrates was developed

Silicon-on-ceramic process: Silicon sheet growth and device development for the large-area silicon sheet task of the low-cost solar array project

The technical feasibility of producing solar cell quality sheet silicon to meet the DOE 1986 cost goal of 70 cents/watt was investigated. The silicon on ceramic approach is to coat a low cost ceramic substrate with large grain polycrystalline silicon by unidirectional solidification of molten silicon. Results and accomplishments are summarized

The Viability and Simplicity of 3D-Printed Construction: A Military Case Study

In November 2019, U.S. Marines, Air Force, and Army Corps of Engineers personnel demonstrated the viability and simplicity of three-dimensionally (3D)-printed construction in a controlled environment at the U.S. Army Engineer Research and Development Center—Construction Engineering Research Laboratory in Champaign, Illinois. The tri-service exercise spanned three days and culminated in the construction of three 1 m × 1 m × 1 m (3 ft × 3 ft × 3 ft) concrete dragon’s teeth (square pyramid military fortifications used to defend against tanks and armored vehicles) and several custom-designed objects. The structural components were printed using a custom-built, gantry-style printer called ACES Lite 2 and a commercially available, proprietary mortar mix. This paper examines the viability of using 3D-printed construction in remote, isolated, and expeditionary environments by considering the benefits and challenges associated with the printing materials, structural design, process efficiency, labor demands, logistical considerations, environmental impact, and project cost. Based on the results of this exercise, 3D-printed construction was found to be faster, safer, less labor-intensive, and more structurally efficient than conventional construction methods: the dragon’s teeth were printed in an average of 57 min each and required only two laborers. However, the use of commercially procured, pre-mixed materials introduced additional cost, logistical burden, and adverse environmental impact as compared to traditional, on-site concrete mixing and production. Finally, this paper suggests future applications and areas of further research for 3D-printed construction