Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) Observed Feeding on Chamaesaracha sp. in Eastern Colorado.

Egg, larval, and adult life stages of Colorado potato beetle, Leptinotarsa decemlineata (Say), were observed feeding on or attached to a previously undocumented host plant belonging to the genus Chamaesaracha in eastern Colorado on July 2017. At one site, L. decemlineata were more abundant on Chamaesaracha sp. than the accepted ancestral host plant, Solanum rostratum (Dunal). While future studies should confirm the ancestral status of the observed L. decemlineata and suitability of Chamaesaracha sp. for completion of development, our observations suggest a need for further characterization of the ancestral host range of L. decemlineata

Exploring the Implementation Process of Technology Adoption In Long-term care Nursing Facilities.

There is little understanding of how long-term care settings implement and adopt technology. The study purpose was to set forth a model that integrates implementation science and technology adoption frameworks and to explore the process of EHR technology implementation leading to adoption. Research questions investigated key stakeholders’ experiences with the implementation, if adoption occurred, and what themes mapped to the new model. There were three components of the dissertation. Based on a critical analysis of the literature, a model was set forth that integrates implementation science and technology adoption frameworks. Next, the experiences of 30 key stakeholders in three nursing homes were explored to understand implementation strategies. The third was one in-depth case study to explore EHR implementation and adoption. The first study was an exploratory qualitative study using grounded theory methods with focus groups (nurses and certified nurse aides) and individual interviews (Directors of Nursing) conducted at three Midwestern nursing homes with various numbers of beds (99-200), locations, and stages of implementation. A stratified random sample was used for focus groups (nurses and certified nurse aides). Data analysis included constant comparison of data. The second study an in-depth case study at a 124 bed, inner-city nursing home. Data sources were interviews of nurses and nurse aides (15), observation sessions of key care events (15), and leadership meetings. Data analysis included using constant comparison of themes and descriptive statistics (activity frequencies and percentages). Integration of data occurred to illustrate the dynamics of implementing and adopting the EHR. Five major themes emerged which included: motivation and EHR adoption, factors that influence the implementation, audit and bi-directional feedback, benefits, and opportunities to improve the EHR. The studies supported the new model with the workflow concept broadened to work processes. The importance of this dissertation is that it added to the knowledge of individual’s and system’s perspectives about implementation and adoption of an EHR in LTC facilities. The study supported the new Integrated Technology Implementation model concepts. Future research that is designed prospectively using this new model is needed. Other types of users should be studied such as administrators, physicians, and residents.PhDNursingUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/113622/1/rhondas_1.pd

Evolutionary diversification of cryophilic Grylloblatta species (Grylloblattodea: Grylloblattidae) in alpine habitats of California

<p>Abstract</p> <p>Background</p> <p>Climate in alpine habitats has undergone extreme variation during Pliocene and Pleistocene epochs, resulting in repeated expansion and contraction of alpine glaciers. Many cold-adapted alpine species have responded to these climatic changes with long-distance range shifts. These species typically exhibit shallow genetic differentiation over a large geographical area. In contrast, poorly dispersing organisms often form species complexes within mountain ranges, such as the California endemic ice-crawlers (Grylloblattodea: Grylloblattidae: <it>Grylloblatta</it>). The diversification pattern of poorly dispersing species might provide more information on the localized effects of historical climate change, the importance of particular climatic events, as well as the history of dispersal. Here we use multi-locus genetic data to examine the phylogenetic relationships and geographic pattern of diversification in California <it>Grylloblatta</it>.</p> <p>Results</p> <p>Our analysis reveals a pattern of deep genetic subdivision among geographically isolated populations of <it>Grylloblatta </it>in California. Alpine populations diverged from low elevation populations and subsequently diversified. Using a Bayesian relaxed clock model and both uncalibrated and calibrated measurements of time to most recent common ancestor, we reconstruct the temporal diversification of alpine <it>Grylloblatta </it>populations. Based on calibrated relaxed clock estimates, evolutionary diversification of <it>Grylloblatta </it>occurred during the Pliocene-Pleistocene epochs, with an initial dispersal into California during the Pliocene and species diversification in alpine clades during the middle Pleistocene epoch.</p> <p>Conclusions</p> <p><it>Grylloblatta </it>species exhibit a high degree of genetic subdivision in California with well defined geographic structure. Distinct glacial refugia can be inferred within the Sierra Nevada, corresponding to major, glaciated drainage basins. Low elevation populations are sister to alpine populations, suggesting alpine populations may track expanding glacial ice sheets and diversify as a result of multiple glacial advances. Based on relaxed-clock molecular dating, the temporal diversification of <it>Grylloblatta </it>provides evidence for the role of a climate-driven species pump in alpine species during the Pleistocene epoch.</p

Grylloblattodea of Canada

The enigmatic insect order Grylloblattodea comprises two described species in Canada, which are limited to the Montane Cordillera and Pacific Maritime ecozones. One of the described species has three Canadian subspecies of uncertain taxonomic ranking, and there are two additional undescribed or unreported species known in close proximity to the Canadian border in western Alberta and British Columbia that likely also occur in Canada. Thus, as much as 50% of the total taxonomic diversity of Grylloblattodea in Canada is still undocumented. Targeted surveys and taxonomic work, as well as studies that describe the ecology and conservation status of Grylloblattodea are important goals for future research

Experimental lithic tool displacement due to long-term animal disturbance

Controlled experiments in lithic technology tend to focus on controlling the human component of lithic tool manufacturing and use; however, animal disturbance can move and alter artifacts in non-random ways, thus altering the behavioral meaning assigned to artifacts and their contexts. The patterning visible in archeological debris on a horizontal plane can provide evidence for activity zones, pathways, and site formation processes. While the effects of trampling actors on the vertical displacement of artifacts have shown that artifacts can be dramatically displaced, the horizontal movement due to trampling is relatively less studied, particularly the effect over extended time periods. Here, an experimental investigation of experimentally produced lithic tools in three contexts with varying degrees of animal trampling intensity is described, and the resulting patterns of artifact displacement are presented. Animal trampling can produce directed, non-random patterning in how artifacts are moved from their original location. The role that bedding slope plays in transport direction given different degrees of activity is also explored. These results show that trampling can produce patterned artifact scatters similar to activity centers and should be taken into consideration for spatial analyses of archeological formation processes

Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) Observed Feeding on Chamaesaracha sp. in Eastern Colorado.

Egg, larval, and adult life stages of Colorado potato beetle, Leptinotarsa decemlineata (Say), were observed feeding on or attached to a previously undocumented host plant belonging to the genus Chamaesaracha in eastern Colorado on July 2017. At one site, L. decemlineata were more abundant on Chamaesaracha sp. than the accepted ancestral host plant, Solanum rostratum (Dunal). While future studies should confirm the ancestral status of the observed L. decemlineata and suitability of Chamaesaracha sp. for completion of development, our observations suggest a need for further characterization of the ancestral host range of L. decemlineata

New experiments and a model-driven approach for interpreting Middle Stone Age Lithic Point Function using the Edge Damage Distribution Method

The Middle Stone Age (MSA) is associated with early evidence for symbolic material culture and complex technological innovations. However, one of the most visible aspects of MSA technologies are unretouched triangular stone points that appear in the archaeological record as early as 500,000 years ago in Africa and persist throughout the MSA. How these tools were being used and discarded across a changing Pleistocene landscape can provide insight into how MSA populations prioritized technological and foraging decisions. Creating inferential links between experimental and archaeological tool use helps to establish prehistoric tool function, but is complicated by the overlaying of post-depositional damage onto behaviorally worn tools. Taphonomic damage patterning can provide insight into site formation history, but may preclude behavioral interpretations of tool function. Here, multiple experimental processes that form edge damage on unretouched lithic points from taphonomic and behavioral processes are presented. These provide experimental distributions of wear on tool edges from known processes that are then quantitatively compared to the archaeological patterning of stone point edge damage from three MSA lithic assemblages--Kathu Pan 1, Pinnacle Point Cave 13B, and Die Kelders Cave 1. By using a model-fitting approach, the results presented here provide evidence for variable MSA behavioral strategies of stone point utilization on the landscape consistent with armature tips at KP1, and cutting tools at PP13B and DK1, as well as damage contributions from post-depositional sources across assemblages. This study provides a method with which landscape-scale questions of early modern human tool-use and site-use can be addressed

RNA interference of three up-regulated transcripts associated with insecticide resistance in an imidacloprid resistant population of Leptinotarsa decemlineata

AbstractThe Colorado potato beetle, Leptinotarsa decemlineata (Say), is a major agricultural pest of potatoes in the Central Sands production region of Wisconsin. Previous studies have shown that populations of L. decemlineata have become resistant to many classes of insecticides, including the neonicotinoid insecticide, imidacloprid. Furthermore, L. decemlineata has multiple mechanisms of resistance to deal with a pesticide insult, including enhanced metabolic detoxification by cytochrome p450s and glutathione S-transferases. With recent advances in the transcriptomic analysis of imidacloprid susceptible and resistant L. decemlineata populations, it is possible to investigate the role of candidate genes involved in imidacloprid resistance. A recently annotated transcriptome analysis of L. decemlineata was obtained from select populations of L. decemlineata collected in the Central Sands potato production region, which revealed a subset of mRNA transcripts constitutively up-regulated in resistant populations. We hypothesize that a portion of the up-regulated transcripts encoding for genes within the resistant populations also encode for pesticide resistance and can be suppressed to re-establish a susceptible phenotype. In this study, a discrete set of three up-regulated targets were selected for RNA interference experiments using a resistant L. decemlineata population. Following the successful suppression of transcripts encoding for a cytochrome p450, a cuticular protein, and a glutathione synthetase protein in a select L. decemlineata population, we observed reductions in measured resistance to imidacloprid that strongly suggest these genes control essential steps in imidacloprid metabolism in these field populations