Full-length and ΔC200 forms of Sgs1p rescue the fast senescence of mutants

<p><b>Copyright information:</b></p><p>Taken from "Evidence that the Sgs1 protein facilitates recombinational repair of telomeres during senescence"</p><p>Nucleic Acids Research 2006;34(2):506-516.</p><p>Published online 20 Jan 2006</p><p>PMCID:PMC1342037.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Full-length , or control vector sequences were integrated at the locus of diploids heterozygous for and null mutations. and spore products without or with the integrated alleles (indicated by an asterisk) were compared in liquid senescence assays (see Materials and Methods). The cumulative population doubling since spore germination, and the density of cells after 22 h of growth, from cultures inoculated with 4 × 10 cells/ml are shown. For each time point, the mean and standard errors for at least three independent spore products for each genotype are indicated. For each experiment, filled diamonds indicate , filled circles indicate , open diamonds indicate with an integrated * allele, and open circles indicate with an integrated * allele, as shown. Rescue by full-length integrated * Lack of rescue by the pRS405 vector used to integrate the * alleles. Rescue by , which encodes an Sgs1p derivative lacking the C-terminal 200 amino acids