Is the magnetic field in the heliosheath laminar or a turbulent bath of bubbles?

All the current global models of the heliosphere are based on the assumption that the magnetic field in the heliosheath, in the region close to the heliopause is laminar. We argue that in that region the heliospheric magnetic field is not laminar but instead consists of magnetic bubbles. Recently, we proposed that the annihilation of the "sectored" magnetic field within the heliosheath as it is compressed on its approach to the heliopause produces the anomalous cosmic rays and also energetic electrons. As a product of the annihilation of the sectored magnetic field, densely-packed magnetic islands/bubbles are produced. These magnetic islands/bubbles will be convected with the ambient flows as the sector region is carried to higher latitudes filling the heliosheath. We further argue that the magnetic islands/bubbles will develop upstream within the heliosheath. As a result, the magnetic field in the heliosheath sector region will be disordered well upstream of the heliopause. We present a 3D MHD simulation with very high numerical resolution that captures the north-south boundaries of the sector region. We show that due to the high pressure of the interstellar magnetic field a north-south asymmetry develops such that the disordered sectored region fills a large portion of the northern part of the heliosphere with a smaller extension in the southern hemisphere. We suggest that this scenario is supported by the following changes that occur around 2008 and from 2009.16 onward: a) the sudden decrease in the intensity of low energy electrons detected by Voyager 2; b) a sharp reduction in the intensity of fluctuations of the radial flow; and c) the dramatic differences in intensity trends between GCRs at V1 and 2. We argue that these observations are a consequence of V2 leaving the sector region of disordered field during these periods and crossing into a region of unipolar laminar field.Comment: 36 pages, 15 figures, submitted to Ap

Considerations In Developing And Utilizing Operator Training Simulators

Effective electric system operation depends on strengthening the relationship between the system operator and the electrical system with its associated control system. This relationship can be developed through a training medium which increases the operator\u27s knowledge of the behavior of the power system under various operating conditions and contingencies, and its response to control actions. An Operator Training Simulator (OTS), which simulates the static and dynamic responses of the operator\u27s power system and his control system, can accomplish these training objectives. The concepts developed in this paper are based on the work of the project team through funding by the Electric Power Research Institute. Copyright © 1983 by The Institute of Electrical and Electronics Engineers, Inc

Extreme Plasma Astrophysics

This is a science white paper submitted to the Astro-2020 and Plasma-2020 Decadal Surveys. The paper describes the present status and emerging opportunities in Extreme Plasma Astrophysics -- a study of astrophysically-relevant plasma processes taking place under extreme conditions that necessitate taking into account relativistic, radiation, and QED effects.Comment: A science white paper submitted to the Astro-2020 and Plasma-2020 Decadal Surveys. 7 pages including cover page and references. Paper updated in late March 2019 to include a several additional co-authors and references, and a few small change

A Crystal Structure of the Bifunctional Antibiotic Simocyclinone D8, Bound to DNA Gyrase

Simocyclinones are bifunctional antibiotics that inhibit bacterial DNA gyrase by preventing DNA binding to the enzyme. We report the crystal structure of the complex formed between the N-terminal domain of the Escherichia coli gyrase A subunit and simocyclinone D8, revealing two binding pockets that separately accommodate the aminocoumarin and polyketide moieties of the antibiotic. These are close to, but distinct from, the quinolone-binding site, consistent with our observations that several mutations in this region confer resistance to both agents. Biochemical studies show that the individual moieties of simocyclinone D8 are comparatively weak inhibitors of gyrase relative to the parent compound, but their combination generates a more potent inhibitor. Our results should facilitate the design of drug molecules that target these unexploited binding pockets

MULTIPLE CURRENT SHEET SYSTEMS IN THE OUTER HELIOSPHERE: ENERGY RELEASE AND TURBULENCE

Accepted for publication in The Astrophysical Journal, March 21, 201

Structural Insights into the Quinolone Resistance Mechanism of Mycobacterium tuberculosis DNA Gyrase

Mycobacterium tuberculosis DNA gyrase, an indispensable nanomachine involved in the regulation of DNA topology, is the only type II topoisomerase present in this organism and is hence the sole target for quinolone action, a crucial drug active against multidrug-resistant tuberculosis. To understand at an atomic level the quinolone resistance mechanism, which emerges in extensively drug resistant tuberculosis, we performed combined functional, biophysical and structural studies of the two individual domains constituting the catalytic DNA gyrase reaction core, namely the Toprim and the breakage-reunion domains. This allowed us to produce a model of the catalytic reaction core in complex with DNA and a quinolone molecule, identifying original mechanistic properties of quinolone binding and clarifying the relationships between amino acid mutations and resistance phenotype of M. tuberculosis DNA gyrase. These results are compatible with our previous studies on quinolone resistance. Interestingly, the structure of the entire breakage-reunion domain revealed a new interaction, in which the Quinolone-Binding Pocket (QBP) is blocked by the N-terminal helix of a symmetry-related molecule. This interaction provides useful starting points for designing peptide based inhibitors that target DNA gyrase to prevent its binding to DNA

A Symmetric Dual Feedback System Provides a Robust and Entrainable Oscillator

Many organisms have evolved molecular clocks to anticipate daily changes in their environment. The molecular mechanisms by which the circadian clock network produces sustained cycles have extensively been studied and transcriptional-translational feedback loops are common structures to many organisms. Although a simple or single feedback loop is sufficient for sustained oscillations, circadian clocks implement multiple, complicated feedback loops. In general, different types of feedback loops are suggested to affect the robustness and entrainment of circadian rhythms

High Confidence Prediction of Essential Genes in Burkholderia Cenocepacia

BACKGROUND: Essential genes are absolutely required for the survival of an organism. The identification of essential genes, besides being one of the most fundamental questions in biology, is also of interest for the emerging science of synthetic biology and for the development of novel antimicrobials. New antimicrobial therapies are desperately needed to treat multidrug-resistant pathogens, such as members of the Burkholderia cepacia complex. METHODOLOGY/PRINCIPAL FINDINGS: We hypothesize that essential genes may be highly conserved within a group of evolutionary closely related organisms. Using a bioinformatics approach we determined that the core genome of the order Burkholderiales consists of 649 genes. All but two of these identified genes were located on chromosome 1 of Burkholderia cenocepacia. Although many of the 649 core genes of Burkholderiales have been shown to be essential in other bacteria, we were also able to identify a number of novel essential genes present mainly, or exclusively, within this order. The essentiality of some of the core genes, including the known essential genes infB, gyrB, ubiB, and valS, as well as the so far uncharacterized genes BCAL1882, BCAL2769, BCAL3142 and BCAL3369 has been confirmed experimentally in B. cenocepacia. CONCLUSIONS/SIGNIFICANCE: We report on the identification of essential genes using a novel bioinformatics strategy and provide bioinformatics and experimental evidence that the large majority of the identified genes are indeed essential. The essential genes identified here may represent valuable targets for the development of novel antimicrobials and their detailed study may shed new light on the functions required to support life