Suppression of Argonaute 2 Transcript Levels in Du182A Cells

RNA interference (RNAi) uses double-stranded RNA (dsRNA) molecules to degrade and suppress the transcript level of a complementary mRNA target1. The RNAi pathway is complex and includes many different proteins, like argonautes, in the core machinery. Argonautes are dsRNA binding proteins which help recognize and cleave target mRNA molecules. In our experiments, we attempted to suppress the transcript level of argonaute 2 (Ago2) in a Diabrotica undecimpunctata cell line (Du182A) using dsRNA, with the idea of disrupting the RNAi pathway using an RNAi of RNAi technique. Ago2 transcript levels were suppressed following treatment with dsRNA. Future experiments can now use this technique, with some modification to better understand the RNAi pathway

Brief Communication: Intertooth and Intrafacet Dental Microwear Variation in an Archaeological Sample of Modern Humans From the Jordan Valley

Dental microwear was recorded in a Bronze-Iron Age (3570–3000 BP) sample of modern humans recovered from Tell es-Sa'idiyeh in the Jordan Valley. Microwear patterns were compared between mandibular molars, and between the upper and lower part of facet 9. The comparison revealed a greater frequency of pits and shorter scratches on the second and third molars, compared to the first. Pit frequency also increased on the lower part of the facet on the first molar, compared to the upper part. These results support previous calls for standardization when selecting a molar type for a diet-microwear study. Otherwise the microwear variations along the tooth row could mask any diet-microwear correlations. The results also suggest that there may be a need to choose a consistent location on a facet in order to enhance comparability among studies

Herausforderungen für Hochschulen und Unternehmen durch die Generation Y: Zumutungen und Chancen durch die neue Generation Studierender und Arbeitnehmer

Hochschulen und Unternehmen stehen einer neuen Generation, der so genannten Generation Y gegenüber, wie die nach 1980 Geborenen genannt werden. Als Bewerber und Studierende bei Hochschulen und als potentielle und aktuelle Arbeitnehmer von Unternehmen, sind Veränderungen im Bewerbungs- und Arbeitsverhalten sichtbar. Risikoavers, egoistisch und effizient, aber auch weltoffen und tolerant sollen die jungen Menschen sein. Generationenforscher sind sich einig über den gemessenen Wertewandel. Allein die Hochschulen und rekrutierende Unternehmen sind noch überfordert mit der neuen Generation und deren Ansprüchen. Jammern über das Versagen tradierter Ausbildungsund Personalentwicklungskonzepte gilt nicht. Eine aktive Beschäftigung mit der aktuellen Generation und deren Werten ist nötig und ante portas stehen bereits die nach 2000 Geborenen, die Generation Z

Effect of Rurality on Diabetes-Related Hospitalizations in a Small Acute Care Hospital in Jackson County, Indiana

Patient access to healthcare can be a potentially significant barrier to overall health outcomes, especially when paired with a costly and complex disease state such as diabetes. The purpose of this study was to determine if patients with Type 2 diabetes mellitus living in rural areas of Jackson County experience greater health disparity and more diabetes-related hospitalizations than those living in non-rural areas. The study was conducted at Schneck Medical Center in Seymour, Indiana. A total of 164 patients were included in the final analysis based on the presence of a diabetes-related diagnosis code in the patient’s chart; of these, 53 were considered rural and 111 were considered not rural. The primary outcome was difference in diabetes-related hospitalizations between rural vs non-rural patients. Secondary outcomes included difference in length of stay and difference in total medical charges

O Partograma como instrumento de análise da assistência ao parto

Both the World Health Organization and the Brazilian Ministry of Health recommend using the partogram to follow labor. The objective of this study was to analyze the use of obstetrical interventions, the types of delivery, and perinatal outcomes according to zones I, II and III of the partogram. This cross-sectional study was performed with 233 low-risk pregnant women between December 2004 and March 2005 at a public maternity hospital located in the city of Itapecerica da Serra, in the state of São Paulo. Comparative analysis was performed using Chi-square and Fischer exact tests. The practices used in the different partogram zones with statistical significance of (p = 0.05) were: bath, movement and walking (zone-III); artificial rupture of the membranes (zone-II) and oxytocin (zone-I). Caesarean sections were performed on 24% of women in zone-III. The interventions occurred at a timely moment, indicating that the partogram is an instrument that can be used as a guide when adopting interventions during labor.La Organización Mundial de la Salud y el Ministerio de la Salud de Brasil recomiendan el uso del partograma para acompañamiento del trabajo de parto. El objetivo del estudio fue analizar el uso de intervenciones obstétricas, el tipo de parto y los resultados perinatales, según las zonas I, II y III del partograma. Estudio transversal realizado con 233 parturientas de bajo riesgo entre diciembre de 2004 y marzo de 2005 en una maternidad pública de Itapecerica de la Serra-SP. Fueron utilizadas las pruebas Chi-cuadrado y Exacta de Fischer para análisis comparativo, adoptándose el valor de p=0,05. Las prácticas utilizadas en las diferentes zonas del partograma con significancia estadística (p=0,05) fueron: baño, movimiento y ambulación (zona-III); rotura artificial de membrana (zona-II); y, oxcitocina (zona-I). La cesariana ocurrió en 24,0% de las parturientes en la zona-III. Las intervenciones ocurrieron en momento oportuno, apuntando que el partograma puede ser un instrumento orientador para la adopción de intervenciones en el trabajo de parto.A Organização Mundial da Saúde e Ministério da Saúde do Brasil recomendam o uso do partograma para acompanhamento do trabalho de parto. O objetivo do estudo foi analisar o uso de intervenções obstétricas, tipo de parto e resultados perinatais, segundo as zonas I, II e III do partograma. Estudo transversal realizado com 233 parturientes de baixo risco entre dezembro de 2004 e março de 2005 em uma maternidade pública de Itapecerica da Serra-SP. Foram utilizados os testes Qui-quadrado e Exato de Fischer para análise comparativa, adotando-se o valor de p=0,05. As práticas utilizadas nas diferentes zonas do partograma com significância estatística (p=0,05) foram: banho, movimento e deambulação (zona-III); rotura artificial de membrana (zona-II); ocitocina (zona-I). A cesariana ocorreu em 24,0% das parturientes na zona-III. As intervenções ocorreram em momento oportuno, apontando que partograma pode ser um instrumento norteador para adoção de intervenções no trabalho de parto

Daniel Pekol v. CSL Behring LLC

USDC for the Eastern District of Pennsylvani