Intrapartale Antibiotikaprophylaxe : Auswirkungen auf das Kind?

Durch die steigende Zahl an Krankheiten wie Asthma, Diabetes und Allergien ist das menschliche Mikrobiom in den Fokus von Bevölkerung und Forschung gerückt. Gleichzeitig erhalten durch die steigende Anzahl einer Sectio caesarea sowie durch die relativ hohe Inzidenz von B-Streptokokken-Trägerinnen immer mehr Frauen Antibiotika unter der Geburt. Diese themengeleitete Arbeit will die Frage beantworten, inwiefern sich eine Antibiotikaprophylaxe unter der Geburt auf das kindliche intestinale Mikrobiom auswirkt. Dazu werden die aktuelle Literatur und die Meinung zweier Experten verwendet. Ziel der Arbeit ist es, Hebammen über die Wirkung von Antibiotika und die Aufgaben des Mikrobioms zu informieren und Empfehlungen für die Praxis auszusprechen. Die Ergebnisse zeigen, dass durch den Einsatz von Antibiotika unter der Geburt eine Veränderung bezüglich Menge und Diversität der Mikroben im Stuhl von Neugeborenen nachgewiesen werden kann. Diese sind unabhängig vom Geburtsmodus. Zudem werden die von der Mutter übertragenen Bakterien durch den Einsatz von Antibiotika selektioniert, sodass vorwiegend resistente Bakterien das Neugeborene kolonisieren. Weiterführende Forschung ist notwendig, um die Langzeitauswirkungen auf die Gesundheit des Kindes aufzuzeigen. Weitere Forschungsgebiete stellen das Stillen als modulierenden Effekt auf das Mikrobiom sowie der Einsatz von Probiotika gegen B-Streptokokken dar

Case report: ETS1 gene deletion associated with a low number of recent thymic emigrants in three patients with Jacobsen syndrome

Jacobsen syndrome is a rare genetic disorder associated with a terminal deletion in chromosome 11. The clinical presentation is variable. Although immunodeficiency has been described in patients with Jacobsen syndrome, a clear genotype-phenotype correlation has not yet been established. Here, we report on the immunologic phenotypes of four patients with Jacobsen syndrome. All four patients showed one or more atypical immunologic features. One patient suffered from recurrent viral infections, two patients had experienced a severe bacterial infection and one had received antibiotic prophylaxis since early childhood. One patient had experienced severe, transient immune dysregulation. Hypogammaglobulinemia and low B cell counts were found in two patients, while the number of recent thymic emigrants (CD31+CD45RA+ CD4 cells) was abnormally low in three. When considering the six immune-related genes located within the affected part of chromosome 11 (ETS1, TIRAP, FLI1, NFRKB, THYN1, and SNX19), only the ETS1 gene was found be deleted in the three patients with low numbers of recent thymic emigrants and non-switched memory B cells. Our findings support the hypothesis whereby Jacobsen syndrome is associated with a combined immunodeficiency with variable presentation. Further investigations of potential genotype-phenotype correlations are warranted and might help to personalize patient management in individuals lacking immune-related genes. In addition, we recommend immunologic follow-up for all patients with Jacobsen syndrome, as immune abnormalities may develop over time. Keywords: ETS1; Jacobsen syndrome; genetic disorder; immunodeficiency; recent thymic emigrants

Immune and metabolic processes jointly contribute to susceptibility to invasive parasites - The case of Anguillicola crassus in eels

Die Einschleppung gebietsfremder Parasiten durch den Menschen ist einer der Hauptgründe für das Auftreten neuer Krankheiten in the letzten Jahrzehnten. Neue Wirte sind oftmals anfälliger für diese invasiven Parasiten als die ursprünglichen Wirte. In schwerwiegenden Fällen können invasive Parasiten zu Massensterben und zum Aussterben ihrer neuen Wirte führen. Der ursprüngliche Wirt des Aalparasiten Anguillicola crassus ist der Japanische Aal. In den frühen 1980er Jahren wurde der Parasit in die Population des Europäischen Aals eingeschleppt. Er ist einer der Faktoren, die für den Populationsrückgang des Europäischen Aals verantwortlich sind. Die molekularen Prozesse, die zur stärkeren Anfälligkeit des Europäischen Aals im Vergleich zum Japanischen Aal führen, sind noch nicht zureichend bekannt. Die Analyse transkriptomweiter differenzieller Genexpression von Immungewebe ergab, dass im Europäischen Aal sowohl Immun- als auch Nichtimmungene differenziell exprimiert waren. Dies war im Japanischen Aal nicht der Fall und deutet darauf hin, dass der Europäische Aal eine ineffiziente und kostspielige Immunantwort auslöst. Die Resultate ensprechen jenen die schon in anderen Wirbeltierwirt-invasiven Parasiten-Systemen beobachtet wurden. Ausserdem stützen diese Resultate die Hypothese, dass neuen Wirten eine wirksame Immunantwort fehlt und sie deuten darauf hin, dass Nichtimmunprozesse wesentlich zur höheren Anfälligkeit von neuen Wirten beitragen. Als Reaktion of die negativen Fitnesseffekte können neue Wirte Abwehrmechanismen entwickeln. Im Europäischen Aal entspricht das der Einkapselung von A. crassus. Einkapselung führte zu eine niedrigere Abundanz adulter A. crassus. Dies deutet darauf hin, dass das Einkapseln sich positiv auf die Gesundheit infizierter Aale auswirkt. Jedoch war die Abundanz zweier nativer Parasiten höher in Aalen, die A. crassus einkapselten. Eine verbesserte Abwehr des eingeschleppten Parasiten könnte daher die Abwehr nativer Parasiten beeinträchtigen.The human-mediated translocation of non-native parasites into foreign regions is one of the primary factors for the emergence of new diseases in recent decades. Novel hosts are often more susceptible to these invasive parasites than the native host. In severe cases, invasive parasites can lead to population declines and extinctions of their novel hosts. The eel parasite Anguillicola crassus is native to the Japanese eel. In the early 1980s it was introduced into the European eel population and is now considered to be one factor contributing to the population decline of its novel host. The underlying molecular processes determining higher susceptibility in the European eel compared to the Japanese eel are not well understood. Using whole-transcriptome differential gene expression analysis of immune organs, I found that genes involved in both immune and non-immune processes were differentially expressed in the European eel but not the Japanese eel, suggestive of an ineffective and costly immune response in the former. These results are in line with those observed between susceptible and resistant hosts in other vertebrate host-invasive parasite systems. Furthermore, the results support the hypothesis that novel hosts lack an effective immune response. The results also suggest that alteration of non-immune processes contributes substantially higher susceptibilities of novel hosts. In response to the negative fitness effects of invasive parasites, novel hosts can evolve coping mechanisms. The European eel has the capacity to encapsulate and kill A. crassus. Using natural infections, I found a lower abundance of adult A. crassus, the most costly parasitic stage in those eels encapsulating the parasite, suggesting that encapsulation can potentially improve health of infected eels. At the same time, the abundance of two native parasites was higher in those eels encapsulating A. crassus. Thus, coping with A. crassus may come at the expense of coping with native parasites

State of the art of existing breeding initiatives & actions planned to strengthen collaborations

State of the art of existing breeding initiatives3Introduction In order to strengthen organic breeding, it is important to know the state of the art of existing initiatives, programs and networks of organic breeding and breeding for organic, and in what crops most organic breeding is currently conducted. Although the number of organic breeding initiatives are growing, as a whole, organic breeding is still relatively marginal compared to conventional breeding. Next to more financial support, another solution to make organic breeding more effective is by improving collaborations. Collaboration can entail, among others, improved exchange of knowledge (breeding tools and approaches) or the exchange of material. In LIVESEED, several activities have been set up to improve collaboration, such as crop‐specific breeding activities, crop‐group activities and systems‐based breeding approaches. For each of these activities, timelines have been developed to strengthen collaborations. This shall improve on one side the capacity building of existing organic plant breeding programs for respective crops and help to identify breeding gaps for those crops, where no activity could be mapped so far

COVID-19 Vaccine Acceptance Among Parents of Children With Multisystem Inflammatory Syndrome in Children

Data on COVID-19 vaccine acceptability among parents of children with multisystem inflammatory syndrome (MIS-C) are limited. In this cohort of children with MIS-C, enrolled in the Swissped RECOVERY trial (NCT04826588), comparing intravenous immunoglobulins or methylprednisolone, who, in accordance with Swiss guidelines, were recommended for SARS-CoV-2 vaccination, 65% (73/112) of parents reported being vaccinated against SARS-CoV-2 before the MIS-C, while 70% were vaccinated after the MIS-C episode of their child. None of the children were vaccinated before the occurrence of the MIS-C, and only 9% (5/56) received the COVID-19 vaccine after the MIS-C. The predominant barriers to COVID-19 vaccination were concerns over potential side effects and insufficient support from their doctors. This emphasizes the crucial role of health care providers in promoting COVID-19 vaccination among children

Partitioning the effects of plant diversity on ecosystem functions at different trophic levels

Biodiversity effects on ecosystem functioning can be partitioned into complementarity effects, driven by many species, and selection effects, driven by few. Selection effects occur through interspecific abundance shifts (dominance) and intraspecific shifts in functioning. Complementarity and selection effects are often calculated for biomass, but very rarely for secondary productivity, i.e. energy transfer to higher trophic levels. We calculated diversity effects for three functions: aboveground biomass, insect herbivory and pathogen infection, the latter two as proxies for energy transfer to higher trophic levels, in a grassland experiment (PaNDiv) manipulating species richness, functional composition, nitrogen enrichment and fungicide treatment. Complementarity effects were on average positive and selection effects negative for biomass production and pathogen infection and multiple species contributed to diversity effects in mixtures. Diversity effects were on average less pronounced for herbivory. Diversity effects for the three functions were not correlated, because different species drove the different effects. Benefits (and costs) from growing in diverse communities, be it reduced herbivore or pathogen damage or increased productivity either due to abundance increases or increased productivity per area were distributed across different plant species, leading to highly variable contributions of single species to effects of diversity on different functions. These results show that different underlying ecological mechanisms can result in similar overall diversity effects across functions.Peer reviewe