CD38: A NAADP degrading enzyme

AbstractThe role of the multifunctional enzyme CD38 in formation of the Ca2+-mobilizing second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) was investigated. Gene silencing of CD38 did neither inhibit NAADP synthesis in intact Jurkat T cells nor in thymus or spleen obtained from CD38 knock out mice. In vitro, both NAADP formation by base-exchange and degradation to 2-phospho adenosine diphosphoribose were efficiently decreased. Thus in vivo CD38 appears to be a NAADP degrading rather than a NAADP forming enzyme, perhaps avoiding desensitizing NAADP levels in intact cells

Indirect contact predicts direct contact : longitudinal evidence and the mediating role of intergroup anxiety

While the effects of direct and indirect forms of contact on intergroup relations are well documented, little is known about their longitudinal co-development. Based on the social-psychological literature, we hypothesize that indirect contact predicts future direct contact by reducing intergroup anxiety. Across five longitudinal studies (Study 1: German adults, N = 560; Study 2: German, Dutch, and Swedish school students, N = 6,600; Study 3: Northern Irish children, N = 1,593; Study 4: Northern Irish adults, N = 404; Study 5: German adults, N = 735), we systematically examined this effect, and further tested the mediating role of intergroup anxiety in Studies 3 to 5. Cross-lagged models provided consistent evidence for the positive effect of indirect contact on future direct contact, while a reduction in intergroup anxiety mediates this effect in most models. Results highlight the importance of indirect contact, which has the potential to increase direct contact, and thus promote social cohesion in diverse contexts, over time.PostprintPeer reviewe

Difficulties faced by physicians from four European countries in rebutting anti-vaccination arguments::A cross-sectional study

Introduction:Physicians play a critical role in encouraging their patients to get vaccinated, in part by responding to patients’ concerns about vaccines. It is therefore important to understand what difficulties physicians have in dealing with different concerns they may encounter. The aim of this article was to determine physicians’ perceptions of difficulties in rebutting different anti-vaccination arguments from patients using data collected as part of a cross-sectional, cross-national questionnaire on physicians’ vaccine attitudes and behaviours.Methods:Physicians in four European countries (Finland, Germany, France, and Portugal, total n = 2,718) rated 33 different arguments, chosen to represent 11 different psychological motivations underlying vaccine hesitancy, in terms of their perceptions of how difficult each argument would be to rebut.Results:Across all countries, physicians perceived arguments based on religious concerns and “reactance” (i.e., resistance to perceived curbs of freedom) to be the most difficult to rebut, whereas arguments based on patients’ distorted perception of the risks of disease and vaccines were perceived to be the easiest. There were also between-country differences in the level of perceived difficulty of argument rebuttal. Physicians’ perceived difficulty with rebutting arguments was significantly negatively correlated with their vaccine recommendation behaviours and their preparedness for vaccination discussions.Conclusions:Physicians may feel better equipped to counter arguments that can be rebutted with facts and evidence, but may struggle to respond when arguments are motivated by psychological dispositions or values

Healthcare professionals’ attitudes to mandatory COVID-19 vaccination::Cross-sectional survey data from four European countries

Mandatory vaccinations are widely debated since they restrict individuals’ autonomy in their health decisions. As healthcare professionals (HCPs) are a common target group of vaccine mandates, and also form a link between vaccination policies and the public, understanding their attitudes toward vaccine mandates is important. The present study investigated physicians’ attitudes to COVID-19 vaccine mandates in four European countries: Finland, France, Germany, and Portugal. An electronic survey assessing attitudes to COVID-19 vaccine mandates and general vaccination attitudes (e.g., perceived vaccine safety, trust in health authorities, and openness to patients) was sent to physicians in the spring of 2022. A total of 2796 physicians responded. Across all countries, 78% of the physicians were in favor of COVID-19 vaccine mandates for HCPs, 49% favored COVID-19 vaccine mandates for the public, and 67% endorsed COVID-19 health passes. Notable differences were observed between countries, with attitudes to mandates found to be more positive in countries where the mandate, or similar mandates, were in effect. The associations between attitudes to mandates and general vaccination attitudes were mostly small to neglectable and differed between countries. Nevertheless, physicians with more positive mandate attitudes perceived vaccines as more beneficial (in Finland and France) and had greater trust in medical authorities (in France and Germany). The present study contributes to the body of research within social and behavioral sciences that support evidence-based vaccination policymaking

Endorsement of alternative medicine and vaccine hesitancy among physicians: A cross-sectional study in four European countries

Vaccine hesitancy has become a threat to public health, especially as it is a phenomenon that has also been observed among healthcare professionals. In this study, we analyzed the relationship between endorsement of complementary and alternative medicine (CAM) and vaccination attitudes and behaviors among healthcare professionals, using a cross-sectional sample of physicians with vaccination responsibilities from four European countries: Germany, Finland, Portugal, and France (total N = 2,787). Our results suggest that, in all the participating countries, CAM endorsement is associated with lower frequency of vaccine recommendation, lower self-vaccination rates, and being more open to patients delaying vaccination, with these relationships being mediated by distrust in vaccines. A latent profile analysis revealed that a profile characterized by higher-than-average CAM endorsement and lower-than-average confidence and recommendation of vaccines occurs, to some degree, among 19% of the total sample, although these percentages varied from one country to another: 23.72% in Germany, 17.83% in France, 9.77% in Finland, and 5.86% in Portugal. These results constitute a call to consider health care professionals’ attitudes toward CAM as a factor that could hinder the implementation of immunization campaigns

Difficulties faced by physicians from four European countries in rebutting anti-vaccination arguments

Physicians play a critical role in encouraging their patients to get vaccinated, in part by responding to patients' concerns about vaccines. It is therefore important to understand what difficulties physicians have in dealing with different concerns they may encounter. We investigated physicians' perceptions of difficulties in rebutting different anti-vaccination arguments from patients using data collected as part of a cross-sectional, cross-national questionnaire on physicians' vaccine attitudes and behaviours. Physicians in four European countries (Finland, Germany, France, and Portugal, total n = 2,718) rated 33 different arguments, chosen to represent 11 different psychological motivations, in terms of their perceptions of how difficult each argument would be to rebut. Across all countries, physicians perceived arguments based on religious concerns and “reactance” (i.e., resistance to perceived curbs of freedom) to be the most difficult to rebut, whereas arguments based on patients' distorted perception of the risks of disease and vaccines were perceived as the easiest. There were also between-country differences in the level of perceived difficulty of argument rebuttal. Physicians' perceived difficulty with rebutting arguments was significantly negatively correlated with their vaccine recommendation behaviours and their preparedness for vaccination discussions. Physicians may feel better equipped to counter arguments that can be rebutted with facts and evidence, but may need support to respond when arguments are motivated by more complex psychological dispositions

Incomplete cytokinesis and re-fusion of small mononucleated Hodgkin cells lead to giant multinucleated Reed-Sternberg cells

Multinucleated Reed-Sternberg (RS) cells are pathognomonic for classical Hodgkin lymphoma (HL), and their presence is essential for diagnosis. How these giant tumor cells develop is controversial, however. It has been postulated that RS cells arise from mononucleated Hodgkin cells via endomitosis. Conversely, continuous single-cell tracking of HL cell lines by long-term time-lapse microscopy has identified cell fusion as the main route of RS cell formation. In contrast to growth-induced formation of giant Hodgkin cells, fusion of small mononuclear cells followed by a size increase gives rise to giant RS cells. Of note, fusion of cells originating from the same ancestor, termed re-fusion, is seen nearly exclusively. In the majority of cases, re-fusion of daughter cells is preceded by incomplete cytokinesis, as demonstrated by microtubule bonds among the cells. We confirm at the level of individual tracked cells that giant Hodgkin and RS cells have little proliferative capacity, further supporting small mononuclear Hodgkin cells as the proliferative compartment of the HL tumor clone. In addition, sister cells show a shared propensity for re-fusion, providing evidence of early RS cell fate commitment. Thus, RS cell generation is related neither to cell fusion of unrelated Hodgkin cells nor to endomitosis, but rather is mediated by re-fusion of daughter cells that underwent mitosis. This surprising finding supports the existence of a unique mechanism for the generation of multinuclear RS cells that may have implications beyond HL, given that RS-like cells are frequently observed in several other lymphoproliferative diseases as well