18 research outputs found
Interactions between proteins bound to biomembranes
We study a physical model for the interaction between general inclusions
bound to fluid membranes that possess finite tension, as well as the usual
bending rigidity. We are motivated by an interest in proteins bound to cell
membranes that apply forces to these membranes, due to either entropic or
direct chemical interactions. We find an exact analytic solution for the
repulsive interaction between two similar circularly symmetric inclusions. This
repulsion extends over length scales of order tens of nanometers, and contrasts
with the membrane-mediated contact attraction for similar inclusions on
tensionless membranes. For non circularly symmetric inclusions we study the
small, algebraically long-ranged, attractive contribution to the force that
arises. We discuss the relevance of our results to biological phenomena, such
as the budding of caveolae from cell membranes and the striations that are
observed on their coats.Comment: 22 pages, 2 figure
Measuring proper motions of isolated neutron stars with Chandra
The excellent spatial resolution of the Chandra observatory offers the
unprecedented possibility to measure proper motions at X-ray wavelength with
relatively high accuracy using as reference the background of extragalactic or
remote galactic X-ray sources. We took advantage of this capability to
constrain the proper motion of RX J0806.4-4123 and RX J0420.0-5022, two X-ray
bright and radio quiet isolated neutron stars (INSs) discovered by ROSAT and
lacking an optical counterpart. In this paper, we present results from a
preliminary analysis from which we derive 2 sigma upper limits of 76 mas/yr and
138 mas/yr on the proper motions of RX J0806.4-4123 and RX J0420.0-5022
respectively. We use these values together with those of other ROSAT discovered
INSs to constrain the origin, distance and evolutionary status of this
particular group of objects. We find that the tangential velocities of radio
quiet ROSAT neutron stars are probably consistent with those of 'normal'
pulsars. Their distribution on the sky and, for those having accurate proper
motion vectors, their possible birth places, all point to a local population,
probably created in the part of the Gould Belt nearest to the earth.Comment: 8 pages, 3 figures, to appear in Astrophysics and Space Science, in
the proceedings of "Isolated Neutron Stars: from the Interior to the
Surface", edited by D. Page, R. Turolla and S. Zan
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Energetic particle influence on the Earth's atmosphere
This manuscript gives an up-to-date and comprehensive overview of the effects of energetic particle precipitation (EPP) onto the whole atmosphere, from the lower thermosphere/mesosphere through the stratosphere and troposphere, to the surface. The paper summarizes the different sources and energies of particles, principally
galactic cosmic rays (GCRs), solar energetic particles (SEPs) and energetic electron precipitation (EEP). All the proposed mechanisms by which EPP can affect the atmosphere
are discussed, including chemical changes in the upper atmosphere and lower thermosphere, chemistry-dynamics feedbacks, the global electric circuit and cloud formation. The role of energetic particles in Earth’s atmosphere is a multi-disciplinary problem that requires expertise from a range of scientific backgrounds. To assist with this synergy, summary tables are provided, which are intended to evaluate the level of current knowledge of the effects of energetic particles on processes in the entire atmosphere
Curriculum Design and Scholarship for New Educators: A Professional Development Workshop for Medical Students
Introduction: Medical students' professional development includes their role as educators. Despite greater opportunities to join medical education curriculum development, medical students' engagement in these activities remains limited. A recent national study on student leadership in curricular change revealed a formal lack of leadership and training in medical education as significant barriers. Medical students' unawareness of how to disseminate curricula as educational scholarship and its value to their careers also restricts the fullness of their formation as educators.
Methods: We designed a 3-hour, interactive, project-focused conference workshop for medical students without prior knowledge in curriculum development. Of participants, 64 worked in 10 groups creating medical curricula using Kern's six-step approach in student-facilitated breakout sessions. Completed group projects were presented, including brief action plans for transforming their work into scholarship. The workshop was evaluated using a mixed-methods approach.
Results: Of survey respondents, 44 mostly medical students, faculty, and administrators from different institutions rated the workshop as a very positive experience, and the pacing of the breakout groups as effective. A notable increase in self-reported mastery, as measured by learning objectives aligned with Kern's six-step model, was recorded from student respondents as compared to faculty. A sense of readiness to participate in curricular decisions either at the home institution or in individual career paths was evident from narrative comments.
Discussion: Our workshop provided medical students with a foundation in curriculum development and educational scholarship. Session design provided flexibility in the pace of breakout sessions and allowed in-depth discussion of educational topics
A conserved carboxy-terminal domain in the major tegument structural protein VP22 facilitates virion packaging of a chimeric protein during productive herpes simplex virus 1 infection
AbstractRecombinant virus HSV-1(RF177) was previously generated to examine tegument protein VP22 function by inserting the GFP gene into the gene encoding VP22. During a detailed analysis of this virus, we discovered that RF177 produces a novel fusion protein between the last 15 amino acids of VP22 and GFP, termed GCT-VP22. Thus, the VP22 carboxy-terminal specific antibody 22-3 and two anti-GFP antibodies reacted with an approximately 28 kDa protein from RF177-infected Vero cells. GCT-VP22 was detected at 1 and 3 hpi. Examination of purified virions indicated that GCT-VP22 was incorporated into RF177 virus particles. These observations imply that at least a portion of the information required for virion targeting is located in this domain of VP22. Indirect immunofluorescence analyses showed that GCT-VP22 also localized to areas of marginalized chromatin during RF177 infection. These results indicate that the last fifteen amino acids of VP22 participate in virion targeting during HSV-1 infection