Multiple Migration and Use of Ties: Bangladeshis in Italy and Beyond

This article analyses previous multiple migratory trajectories of Bangladeshifirst generationmigrants before their arrival in Italy and within Italy. It also uncovers the role of social net-works and transnational ties in their multiple migrations. Thefindings show that theirfirstinternational migration was mainly shaped by their family\u2019s socio-economic condition andtransnational kinship networks. They already had someone from their family or close relativesin the preferred country with whom they were connected. Their onward relocations until arriv-ing in Italy, in most cases, was to achieve the socio-economic success and legal status thatthey had failed to attain in theirfirst and subsequent destinations, but the transnational connec-tions with friends or acquaintances are a key resource facilitating these remigrations. Banglade-shis who arrived in Italy from various countries mostly had networks, either with someonefrom their local district in Bangladesh or with their earlier fellow migrants who moved to Italybefore the

[Introduction] The making and unmaking of precarious, ideal subjects – migration brokerage in the Global South

The migration literature is often underpinned by the idea that migrants are either completely ‘free’ agents, individually choosing how best to achieve returns on their human capital and resources (Sjaastad 1962) or ‘agents of development’ for their home countries and regions (Turner and Kleist 2013). Conversely they are viewed as exploited slaves, being pushed into low-paid occupations and controlled by middlemen and employers. Unsurprisingly, in many close-knit societies a process as expensive and life-defining as migration is rarely undertaken as an individual act and is shaped by complex social interactions within kinship networks and beyond (Lindquist 2012). Brokerage is ever-present in migrant labour markets around the world, variously interpreted as occupying the ‘middle space’ between migrants and the state, helping migrants navigate complex immigration regimes (Lindquist, Xiang, and Yeoh 2012; McKeown 2012; Schapendonk 2017), acting as an extension of the state seeking to outsource border controls (Goh, Wee, and Yeoh 2017) and colluding with employers to cheapen and commoditise migrant labour (Guérin 2013; McCollum and Findlay 2018). It is increasingly recognised that an understanding of contemporary migration is not complete without an understanding of the mediating practices that facilitate and constrain it (Coe and Jordhus-Lier 2011; Cranston, Schapendonk, and Spaan 2018). This special issue investigates the role that migration brokers play in the subjectivation and precarisation of migrant men and women from marginalised classes and ethnicities in the Global South. It shows how these processes are critical for them to become a part of contemporary economic and political systems of international and internal labour circulation. It responds to the call of labour geographers for a deeper understanding of the ways in which diverse economic and social contexts result in complex forms of precarity (McDowell 2015) and adds to the evidence on the role of actors beyond the workplace in co-creating precarity (Buckley, McPhee, and Rogaly 2017)

Comparison of sequencing methods and data processing pipelines for whole genome sequencing and minority single nucleotide variant (mSNV) analysis during an influenza A/H5N8 outbreak

As high-throughput sequencing technologies are becoming more widely adopted for analysing pathogens in disease outbreaks there needs to be assurance that the different sequencing technologies and approaches to data analysis will yield reliable and comparable results. Conversely, understanding where agreement cannot be achieved provides insight into the limitations of these approaches and also allows efforts to be focused on areas of the process that need improvement. This manuscript describes the next-generation sequencing of three closely related viruses, each analysed using different sequencing strategies, sequencing instruments and data processing pipelines. In order to determine the comparability of consensus sequences and minority (sub-consensus) single nucleotide variant (mSNV) identification, the biological samples, the sequence data from 3 sequencing platforms and the *.bam quality-trimmed alignment files of raw data of 3 influenza A/H5N8 viruses were shared. This analysis demonstrated that variation in the final result could be attributed to all stages in the process, but the most critical were the well-known homopolymer errors introduced by 454 sequencing, and the alignment processes in the different data processing pipelines which affected the consistency of mSNV detection. However, homopolymer errors aside, there was generally a good agreement between consensus sequences that were obtained for all combinations of sequencing platforms and data processing pipelines. Nevertheless, minority variant analysis will need a different level of careful standardization and awareness about the possible limitations, as shown in this study

Net grassland carbon flux over a subambient to superambient CO2 gradient

Increasing atmospheric CO2 concentrations may have a profound effect on the structure and function of plant communities. A previously grazed, central Texas grassland was exposed to a 200-µmol mol-1 to 550 µmol mol-1 CO2 gradient from March to mid-December in 1998 and 1999 using two, 60-m long, polyethylene-covered chambers built directly onto the site. One chamber was operated at subambient CO2 concentrations (200-360 µmol mol-1 daytime) and the other was regulated at superambient concentrations (360-550 µmol mol-1). Continuous CO2 gradients were maintained in each chamber by photosynthesis during the day and respiration at night. Net ecosystem CO2 flux and end-of-year biomass were measured in each of 10, 5-m long sections in each chamber. Net CO2 fluxes were maximal in late May (c. day 150) in 1998 and in late August in 1999 (c. day 240). In both years, fluxes were near zero and similar in both chambers at the beginning and end of the growing season. Average daily CO2 flux in 1998 was 13 g CO2 m-2 day-1 in the subambient chamber and 20 g CO2 m-2 day-1 in the superambient chamber; comparable averages were 15 and 26 g CO2 m-2 day-1 in 1999. Flux was positively and linearly correlated with end-of-year above-ground biomass but flux was not linearly correlated with CO2 concentration; a finding likely to be explained by inherent differences in vegetation. Because C3 plants were the dominant functional group, we adjusted average daily flux in each section by dividing the flux by the average percentage C3 cover. Adjusted fluxes were better correlated with CO2 concentration, although scatter remained. Our results indicate that after accounting for vegetation differences, CO2 flux increased linearly with CO2 concentration. This trend was more evident at subambient than superambient CO2 concentrations

Norovirus outbreak in a natural playground: A One Health approach

Norovirus constitutes the most frequently identified infectious cause of disease outbreaks associated with untreated recreational water. When investigating outbreaks related to surface water, a One Health approach is insightful. Historically, there has been a focus on potential contamination of recreational water by bird droppings and a recent publication demonstrating human noroviruses in bird faeces suggested this should be investigated in future water-related norovirus outbreaks. Here, we describe a One Health approach investigating a norovirus outbreak in a natural playground. On social media, a large amount of waterfowl were reported to defecate near these playground premises leading to speculations about their potential involvement. Surface water, as well as human and bird faecal specimens, was tested for human noroviruses. Norovirus was found to be the most likely cause of the outbreak but there was no evidence for transmission via waterfowl. Cases had become known on social media prior to notification to the public health service underscoring the potential of online media as an early warning system. In view of known risk factors, advice was given for future outbreak investigations and natural playgroun

COVID-19 in health-care workers in three hospitals in the south of the Netherlands:A cross-sectional study

Background: 10 days after the first reported case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the Netherlands (on Feb 27, 2020), 55 (4%) of 1497 health-care workers in nine hospitals located in the south of the Netherlands had tested positive for SARS-CoV-2 RNA. We aimed to gain insight in possible sources of infection in health-care workers. Methods: We did a cross-sectional study at three of the nine hospitals located in the south of the Netherlands. We screened health-care workers at the participating hospitals for SARS-CoV-2 infection, based on clinical symptoms (fever or mild respiratory symptoms) in the 10 days before screening. We obtained epidemiological data through structured interviews with health-care workers and combined this information with data from whole-genome sequencing of SARS-CoV-2 in clinical samples taken from health-care workers and patients. We did an in-depth analysis of sources and modes of transmission of SARS-CoV-2 in health-care workers and patients. Findings: Between March 2 and March 12, 2020, 1796 (15%) of 12 022 health-care workers were screened, of whom 96 (5%) tested positive for SARS-CoV-2. We obtained complete and near-complete genome sequences from 50 health-care workers and ten patients. Most sequences were grouped in three clusters, with two clusters showing local circulation within the region. The noted patterns were consistent with multiple introductions into the hospitals through community-acquired infections and local amplification in the community. Interpretation: Although direct transmission in the hospitals cannot be ruled out, our data do not support widespread nosocomial transmission as the source of infection in patients or health-care workers. Funding: EU Horizon 2020 (RECoVer, VEO, and the European Joint Programme One Health METASTAVA), and the National Institute of Allergy and Infectious Diseases, National Institutes of Health

Metagenomic survey for viruses in Western Arctic caribou, Alaska, through iterative assembly of taxonomic units

Pathogen surveillance in animals does not provide a sufficient level of vigilance because it is generally confined to surveillance of pathogens with known economic impact in domestic animals and practically nonexistent in wildlife species. As most (re-)emerging viral infections originate from animal sources, it is important to obtain insight into viral pathogens present in the wildlife reservoir from a public health perspective. When monitoring living, free-ranging wildlife for viruses, sample collection can be challenging and availability of nucleic acids isolated from samples is often limited. The development of viral metagenomics platforms allows a more comprehensive