Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA

We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis elegans the hSUV3 orthologue is necessary for viability of early embryos. Using immunofluorescence analysis, an in vitro mitochondrial uptake assay and sub‐fractionation of human mitochondria we show hSuv3p to be a soluble protein localised in the mitochondrial matrix. We expressed and purified recombinant hSuv3p protein from a bacterial expression system. The purified enzyme was capable of hydrolysing ATP with a Km of 41.9 µM and the activity was only modestly stimulated by polynucleotides. hSuv3p unwound partly hybridised dsRNA and dsDNA structures with a very strong preference for the latter. The presented analysis of the hSuv3p NTPase/helicase suggests that new functions of the protein have been acquired in the course of evolution

When combat prevents PTSD symptoms—results from a survey with former child soldiers in Northern Uganda

Weierstall R, Schalinski I, Crombach A, Hecker T, Elbert T. When combat prevents PTSD symptoms—results from a survey with former child soldiers in Northern Uganda. BMC Psychiatry. 2012;12(1): 41

Is the Implicit Association Test for Aggressive Attitudes a Measure for Attraction to Violence or Traumatization?

Traumatic exposure is particularly devastating for those who, at a young age, have become combatants or experienced massive adversity after abduction by armed movements. We investigated the impact of traumatic stressors on psychopathology among war-affected young men of Northern Uganda, including former child soldiers. Adaptation to violent environments and coping with trauma-related symptoms often result in an increasing appetite for violence. We analyze implicit attitudes toward violence, assessed by an Implicit Association Test (IAT), among 64 male participants. Implicit attitudes varied as a function of the number of experienced traumatic event types and committed offense types. As the number of traumatic experiences and violence exposure increased, more appetitive aggression was reported, whereas the IAT indicated increasingly negative implicit attitudes toward aggression. The IAT was also the strongest predictor of cortisol levels. Diffusion-model analysis was the best way to demonstrate IAT validity. Implicit measures revealed the trauma-related changes of cognitive structures

The non-ballistic superluminal motion in the plane of the sky-II

The model of non-ballistic jet motion proposed in 2008 provides a simple explanation to the inward jet motion and bent jet. Recently, evidences of such a non-radial motion increase rapidly, and more complicated morphologies appear. On the other hand, the ballistic plus precession model likely holds in majority samples of jet motion. This paper discusses the relationship between the ballistic and non-ballistic model of jet motion, which suggests that the interaction of ejectors with ambient matter can produce knots at different stages of evolution and hence different separations to the core. And as a jet precesses, knots produced between the core and the deceleration radius result in spiral pattern expected by the model of ballistic plus precession; and knots generated at the deceleration radius display non-radial motion such as bent jet or oscillation of ridge-line. This paper develops the first non-ballistic model in four aspects. Firstly, it provides a numerical simulation to the production of multi-knot for a precessing jet. Secondly, it fits the precession behavior of multi-knot and interprets the oscillation of ridge lines like S5 1803+784. Thirdly, it gives an unified interpretation to the bent jet applicable to both multi-knot and single knot. And fourthly, the problem of very large numbers of observed outward motions as opposed to the inward ones is addressed in a new scope.Comment: 9 pages, 6 figures, accepted by MNRA

A One-sided, Highly Relativistic Jet from Cygnus X-3

Very Long Baseline Array images of the X-ray binary, Cygnus X-3, were obtained 2, 4 and 7 days after the peak of a 10 Jy flare on 4 February 1997. The first two images show a curved one-sided jet, the third a scatter-broadened disc, presumably at the position of the core. The jet curvature changes from the first to the second epoch, which strongly suggests a precessing jet. The ratio of the flux density in the approaching to that in the (undetected) receding jet is > 330; if this asymmetry is due to Doppler boosting, the implied jet speed is > 0.81c. Precessing jet model fits, together with the assumptions that the jet is intrinsically symmetric and was ejected during or after the major flare, yield the following constraints: the jet inclination to the line of sight must be < 14 degrees; the cone opening angle must be < 12 degrees; and the precession period must be > 60 days.Comment: 12 pages 7 figures, accepted by Ap

Course of Mental Health in Refugees—A One Year Panel Survey

Background: Cross-sectional studies indicate that a substantial proportion of refugees have psychiatric disorders. However, longitudinal studies on the course of psychiatric symptoms and on influencing factors are scarce. The current study investigates the development of symptoms in an untreated refugee sample in Germany and seeks to identify potential predictors.Methods: Over the course of 1 year, 57 refugees participated in monthly assisted self-reports on the phone assessing emotional distress. At the same time, semi-annual, semi-structured clinical interviews focusing on posttraumatic stress disorder (PTSD) and depression were conducted. The overall dropout rate for the year was 23% for the assisted self-reports and 33% for the clinical interviews.Results: Symptoms did not systematically change over the course of the year. On the individual level, a reliable change in PTSD symptoms was observed in 13% who showed improvement and 24% who showed worsening symptoms. Figures for depression symptoms were 24 and 16% respectively. A higher number of traumatic experiences was related to a greater intensity of PTSD symptoms. In addition, postmigrational stressors were associated with a worsening of PTSD symptoms over the course of the year. Emotional distress was associated with current negative life events, unemployment, and frequent visits to physicians.Conclusions: There is on average no improvement or worsening of symptoms over the period of 1 year. However, individual courses vary, and thus show the importance of risk factors. Accordingly, the identification of risk factors such as trauma load and postmigrational stressors can be useful to determine the need of further monitoring and to provide appropriate interventions when necessary

Orbital dynamics of Cygnus X-3

Orbital-phased-resolved infrared spectra of Cygnus X-3 in outburst and quiescence, including tomographic analysis, are presented. We confirm the phasing of broad HeII and NV lines in quiescence, such that maximum blue shift corresponds to the X-ray minimum at phase = 0.00 +/- 0.04. In outburst, double-peaked HeI structures show a similar phasing with two significant differences: (a) although varying in relative strength, there is continuous line emission in blue and red peaks around the orbit, and (b) an absorption component, ~1/4 of an orbit out of phase with the emission features, is discerned. Doppler tomograms of the double-peaked profiles are consistent with a disk-wind geometry, rotating at velocities of 1000 km/s. Regrettably, the tomography algorithm will produce a similar ring structure from alternative line sources if contaminated by overlying P Cygni profiles. This is certainly the case in the strong 2.0587 micron HeI line, leading to an ambiguous solution for the nature of double-peaked emission. The absorption feature, detected 1/4 of an orbit out of phase with the emission features, is consistent with an origin in the He star wind and yields for the first time a plausible radial velocity curve for the system. We directly derive the mass function of the system, 0.027 M_sun. If we assume a neutron star accretor and adopt a high orbital inclination, i > 60 degrees, we obtain a mass range for the He star of 5 M_sun < M_WR < 11 M_sun. Alternatively if the compact object is a black hole, we estimate M_BH < 10 M_sun. We discuss the implications of these masses for the nature and size of the binary system.Comment: Accepted for publication in ApJ main journa

Sco X-1: The Evolution and Nature of the Twin Compact Radio Lobes

The radio components associated with the LMXB Sco X-1 have been monitored with extensive VLBI imaging at 1.7 and 5.0 GHz over four years, including a 56-hour continuous VLBI observation in 1999 June. We often detected one strong and one weak compact radio component, moving in opposite directions from the radio core. We suggest that the moving components are radio lobes generated by the disruption of energy flow in a twin-beam from the binary system. The average lifetime of a lobe-pair, the space motion of the lobes and the measured energy flow in the beam are discussed in arXiv:astro-ph/0104325. The lobe has a flux density that is variable over a time-scale of one hour, a measured minimum size of 1 mas (2.8 au), and is extended perpendicular to its motion. This short electron radiative lifetime may be caused by synchrotron losses if the lobe magnetic field is 300 G, or by adiabatic expansion of the electrons as soon as they are produced at the working surface. The lobes also show periods of slow expansion and a steepening radio spectrum, perhaps related to the characteristics of the beam energy flow. The radio morphology for Sco X-1 is more simple than for most other Galactic jet sources. The lobes of Sco X-1 are similar to hot-spots found in many extragalactic double sources. Scaling the phenomena observed in Sco X-1 to extragalactic sources implies hot-spot variability time-scales of 10^4 yr and hot-spot lifetimes of 10^5 yr. The recurrent formation of lobes in Sco X-1 probably does not occur for extragalactic radio sources.Comment: 22 pages of text + 16 figures. ApJ, in pres

The Hardness-Intensity Diagram of Cygnus X-3: Revisiting the Radio/X-Ray States

Cygnus X-3 is one of the brightest X-ray and radio sources in the Galaxy, and is well known for its erratic behaviour in X-rays as well as in the radio, occasionally producing major radio flares associated with relativistic ejections. However, even after many years of observations in various wavelength bands Cyg X-3 still eludes clear physical understanding. Studying different emission bands simultaneously in microquasars has proved to be a fruitful approach towards understanding these systems, especially by shedding light on the accretion disc/jet connection. We continue this legacy by constructing a hardness-intensity diagram (HID) from archival Rossi X-ray Timing Explorer data and linking simultaneous radio observations to it. We find that surprisingly Cyg X-3 sketches a similar shape in the HID to that seen in other transient black hole X-ray binaries during outburst but with distinct differences. Together with the results of this analysis and previous studies of Cyg X-3 we conclude that the X-ray states can be assigned to six distinct states. This categorization relies heavily on the simultaneous radio observations and we identify one new X-ray state, the hypersoft state, similar to the ultrasoft state, which is associated to the quenched radio state during which there is no or very faint radio emission. Recent observations of GeV flux observed from Cyg X-3 (Tavani et al. 2009; Fermi LAT Collaboration et al. 2009) during a soft X-ray and/or radio quenched state at the onset of a major radio flare hint that a very energetic process is at work during this time, which is also when the hypersoft X-ray state is observed. In addition, Cyg X-3 shows flaring with a wide range of hardness.Comment: 17 pages, 9 figures, accepted for publication in MNRA

A weak compact jet in a soft state of Cygnus X-1

We present evidence for the presence of a weak compact jet during a soft X-ray state of Cygnus X-1. Very-high-resolution radio observations were taken with the VLBA, EVN and MERLIN during a hard-to-soft spectral state change, showing the hard state jet to be suppressed by a factor of about 3-5 in radio flux and unresolved to direct imaging observations (i.e. < 1 mas at 4 cm). High time-resolution X-ray observations with the RXTE-PCA were also taken during the radio monitoring period, showing the source to make the transition from the hard state to a softer state (via an intermediate state), although the source may never have reached the canonical soft state. Using astrometric VLBI analysis and removing proper motion, parallax and orbital motion signatures, the residual positions show a scatter of ~0.2 mas (at 4 cm) and ~3 mas (at 13 cm) along the position angle of the known jet axis; these residuals suggest there is a weak unresolved outflow, with varying size or opacity, during intermediate and soft X-ray states. Furthermore, no evidence was found for extended knots or shocks forming within the jet during the state transition, suggesting the change in outflow rate may not be sufficiently high to produce superluminal knots.Comment: Accepted in MNRAS; 4 figures and 1 tabl