Positive and psycho-pathological aspects between shame and shamelessness

Interpersonal relationships represent an essential aspect of mental wellbeing and social functioning. If all the symptoms contain a relational meaning, shame represents the relational affect par excellence both in terms of its origin and its purpose. This paper aims to highlight the role of shame as an affect inherent in the rhythmic nature of the encounter with the other, as well as the pathological elements of this aspect in both its conscious and unconscious dimensions. There is a heterogeneous quantitative and qualitative declination of shame, or of the defenses against this affect, among the various pathologies. We consider the fundamental needs of belonging and acceptance and the parallel abandonment anguish from various psychoanalytic and philosophical theoretical perspectives and then analyze the link between their dissatisfaction and the origin of shame. We also touch on the different interpretaions of shame based on eastern and western cultural norms. These hypotheses are closely intertwined with the beliefs of classical psychopathology. The role of the body in the encounter with the other and in the experience of shame is also examined. In particular, we study the role of this affect in schizophrenia, depression, eating disorders, and personality disorders

The AquaGranda digital community memory: activating awareness about climate risk

This study aims to explore how vulnerable communities, scientists, and artists can collaborate to construct digital community memories to excavate opinion spaces related to socio-natural events, creating a shared and inclusive ground for mutual understanding and collective action. The proposed approach combines web archeology and digital artivism to construct community memories that can be used for social activation in relation to climate change. The research draws on participatory and community-based research techniques, citizen-science approaches, and digital methods to collect dispersed community-memory fragments and create an expressive space for the diverse perspectives and needs of the affected populations. It emphasises the importance of inclusivity and non-polarizing approaches for reconstructing and interpreting narrative streams that propagate in the physical and virtual world, and for representing, through digital art, extreme events and their societal impact. To illustrate the proposed approach, the AquaGranda project is here presented as a case study of community memory related to an extreme event: the high tides that occurred in Venice in November 2019

Incorporation of a Non-Conventional Liana Tetracapidium Conophorum Seed Oil Cake in Based - Diets of Indigenous Batéké Chicks Raised in Close Confinement in Congo

The indigenous Batéké hen (Gallus gallus) has low productivity due to various constraints such as feeding sources. The aim of this study was to improve the productivity of the local hen using a new local protein source: Tetracarpidium conophorum meal. Ninety-six chicks 21-day old from the local population were randomized into 3 groups of 32 birds each (TC0, TC4 and TC6), with one replicate. Tetracarpidium conophorum meal was incorporated into groupsTC4 (4% Tcconophorum oil cake and 11% soya oil cake), TC6 (6% T.conophorum oil cake and 9% soya oil cake) whereas TC0   served as a control group (0% T.conophorum meal and 15% soya oilcake). The experiment started at 3 weeks and lasted at 20 weeks of age. Chickens were fed ad libithum and had free access to drinkers. The treatment decreased mortality rate (3.12 % versus 9.3), On the other hand the treatment improves significantly (p ˂ 0.05).  feed intake (56.8 vs 60.1g per day), feed conversion (6.5 vs 5.7), and carcass yield (65.8 % vs 71.3 %). The final body weight at 20 weeks of age was low in all groups (1.200g versus 1300g) at 4% of incorporation of T. conophorum meal. Meat yield. This study indicated to incorporate T.conophorum oil cake at 4% and reducing soya oil cake  from 15% to 11%) in  a small scale poultry farming in Congo

Hepatocyte growth factor and MET support mouse enteric nervous system development, the peristaltic response, and intestinal epithelial proliferation in response to injury

UNLABELLED: Factors providing trophic support to diverse enteric neuron subtypes remain poorly understood. We tested the hypothesis that hepatocyte growth factor (HGF) and the HGF receptor MET might support some types of enteric neurons. HGF and MET are expressed in fetal and adult enteric nervous system. In vitro, HGF increased enteric neuron differentiation and neurite length, but only if vanishingly small amounts (1 pg/ml) of glial cell line-derived neurotrophic factor were included in culture media. HGF effects were blocked by phosphatidylinositol-3 kinase inhibitor and by MET-blocking antibody. Both of these inhibitors and MEK inhibition reduced neurite length. In adult mice, MET was restricted to a subset of calcitonin gene-related peptide-immunoreactive (IR) myenteric plexus neurons thought to be intrinsic primary afferent neurons (IPANs). Conditional MET kinase domain inactivation (Met(fl/fl); Wnt1Cre+) caused a dramatic loss of myenteric plexus MET-IR neurites and 1-1\u27-dioctodecyl-3,3,3\u27,3\u27-tetramethylindocarbocyamine perchlorate (DiI) labeling suggested reduced MET-IR neurite length. In vitro, Met(fl/fl); Wnt1Cre+ mouse bowel had markedly reduced peristalsis in response to mucosal deformation, but normal response to radial muscle stretch. However, whole-bowel transit, small-bowel transit, and colonic-bead expulsion were normal in Met(fl/fl); Wnt1Cre+ mice. Finally, Met(fl/fl); Wnt1Cre+ mice had more bowel injury and reduced epithelial cell proliferation compared with WT animals after dextran sodium sulfate treatment. These results suggest that HGF/MET signaling is important for development and function of a subset IPANs and that these cells regulate intestinal motility and epithelial cell proliferation in response to bowel injury. SIGNIFICANCE STATEMENT: The enteric nervous system has many neuronal subtypes that coordinate and control intestinal activity. Trophic factors that support these neuron types and enhance neurite growth after fetal development are not well understood. We show that a subset of adult calcitonin gene-related peptide (CGRP)-expressing myenteric neurons produce MET, the receptor for hepatocyte growth factor, and that loss of MET activity affects peristalsis in response to mucosal stroking, reduces MET-immunoreactive neurites, and increases susceptibility to dextran sodium sulfate-induced bowel injury. These observations may be relevant for understanding and treating intestinal motility disorders and also suggest that enhancing the activity of MET-expressing CGRP neurons might be a useful strategy to reduce bowel inflammation

The photodecarboxylative addition of carboxylates to phthalimides as a key-step in the synthesis of biologically active 3-arylmethylene-2,3-dihydro-1H-isoindolin-1-ones

The synthesis of various 3-arylmethylene-2,3-dihydro-1H-isoindolin-1-ones was realized following a simple three-step process. The protocol utilized the photodecarboxylative addition of readily available carboxylates to N-(bromoalkyl)phthalimides as a versatile and efficient key step. The initially obtained hydroxyphthalimidines were readily converted to the desired N-diaminoalkylated 3-arylmethylene-2,3-dihydro-1H-isoindolin-1-ones via acid-catalyzed dehydration and subsequent nucleophilic substitution with the corresponding secondary amines. The procedure was successfully applied to the synthesis of known local anesthetics (AL-12, AL-12B and AL-5) in their neutral forms

Incorporation of a Non-Conventional Liana (Tetracapidium conophorum) Seed Oil Cake in Diets of Congolese Indigenous Batéké Chicks Raised Without Outdoor Access

The indigenous Batéké hen (Gallus gallus) has low productivity due to various constraints such as feeding sources. The aim of this study was to improve the productivity of the local hen using a new local protein source: Tetracarpidium conophorum meal.  A sample of ninety-six chicks 21-day old from the local population were randomized into 3 groups of 32 birds each (TC0, TC4 and TC6), with one replicate. In each group. Tetracarpidium conophorum oil cake  was incorporated into groupsTC4 (4% Tcconophorum oil cake and 11% soya oil cake), TC6 (6% T.conophorum oil cake and 9% soya oil cake) whereas TC0   served as a control group (0% T.conophorum meal and 15% soya oilcake). The experiment started at 3 weeks and lasted at 20 weeks of age. Chickens were fed ad libithum and had free access to drinkers. The results  indicated that  mortality rate  decreased (3.12 % versus 9.3),  Also, the treatment improved significantly (p ˂ 0.05)  feed intake (56.8 vs 60.1g per day), feed conversion (6.5 vs 5.7), and carcass yield (65.8 % vs 71.3 %). The final body weight at 20 weeks of age has been improved  groups (1.200g versus 1300g) .. This study indicated  that T.conophorum oil cake  may be useful in diets at 4%  thus reducing soya oil cake  from 15% to 11% of incorporation) in  a small scale poultry farming in Congo

Arrays of Nano-Electromechanical Biosensors Functionalized by Microcontact Printing

The biofunctionalization of nanoelectromechanical structures is critical for the development of new classes of biosensors displaying improved performances and higher-level of integration. We propose a modified microcontact printing method for the functionalization and passivation of large arrays of nanocantilevers in a single, self-aligned step. Using fluorescence microscopy and resonant frequency measurements, we demonstrate (1) the bioactivity and the anti-fouling property of deposited antibodies and BSA molecules and (2) the preservation of the nanostructures' mechanical integrity.Comment: 20 pages, 5 figure

Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse

AbstractHirschsprung Disease (HSCR) is a potentially deadly birth defect characterized by the absence of the enteric nervous system (ENS) in distal bowel. Although HSCR has clear genetic causes, no HSCR-associated mutation is 100% penetrant, suggesting gene–gene and gene-environment interactions determine HSCR occurrence. To test the hypothesis that certain medicines might alter HSCR risk we treated zebrafish with medications commonly used during early human pregnancy and discovered that ibuprofen caused HSCR-like absence of enteric neurons in distal bowel. Using fetal CF-1 mouse gut slice cultures, we found that ibuprofen treated enteric neural crest-derived cells (ENCDC) had reduced migration, fewer lamellipodia and lower levels of active RAC1/CDC42. Additionally, inhibiting ROCK, a RHOA effector and known RAC1 antagonist, reversed ibuprofen effects on migrating mouse ENCDC in culture. Ibuprofen also inhibited colonization of Ret+/− mouse bowel by ENCDC in vivo and dramatically reduced bowel colonization by chick ENCDC in culture. Interestingly, ibuprofen did not affect ENCDC migration until after at least three hours of exposure. Furthermore, mice deficient in Ptgs1 (COX 1) and Ptgs2 (COX 2) had normal bowel colonization by ENCDC and normal ENCDC migration in vitro suggesting COX-independent effects. Consistent with selective and strain specific effects on ENCDC, ibuprofen did not affect migration of gut mesenchymal cells, NIH3T3, or WT C57BL/6 ENCDC, and did not affect dorsal root ganglion cell precursor migration in zebrafish. Thus, ibuprofen inhibits ENCDC migration in vitro and bowel colonization by ENCDC in vivo in zebrafish, mouse and chick, but there are cell type and strain specific responses. These data raise concern that ibuprofen may increase Hirschsprung disease risk in some genetically susceptible children

Suicidal ideation and burnout among psychiatric trainees in Japan

AIM: Burnout is a psychological condition that may occur in all workers after being exposed to excessive work-related stresses. We investigated suicidal ideation and burnout among Japanese psychiatric trainees as a part of the Burnout Syndrome Study (BoSS) International.  METHODS: In the Japanese branch, 91 trainees fully completed suicide ideation and behaviour questionnaire (SIBQ) and Maslach Burnout Inventory-General Survey (MBI-GS).  RESULTS: Passive suicidal ideation was reported by 38.5% of Japanese trainees and 22.0% of them had experienced active suicidal ideation. The burnout rate among Japanese subjects was 40.0%. These results were worse compared to the all 1980 trainees who fully completed the main outcome measure in BoSS International, 25.9%, 20.4% and 36.7%, respectively.  CONCLUSIONS: Our results suggest a higher risk of suicide among Japanese residents. Japan has a higher suicide rate than other countries. Early detection of, and appropriate intervention for, suicidal ideation is important in preventing suicide in psychiatry residents

Is There a Role for Biomarkers in Surveillance of Pancreatic Neuroendocrine Neoplasms in Von Hippel-Lindau Disease?

Von Hippel-Lindau (VHL) disease is an autosomal dominant disorder characterized by the development of multi-organ neoplasms. Among the manifestations of VHL are pancreatic neuroendocrine neoplasms (panNENs). In order to detect these lesions in a timely manner, patients are enrolled in a surveillance program, in accordance with the several existing VHL guidelines. However, these guidelines remain unclear about the role of biomarkers in diagnosing panNENs, despite the benefits a biomarker may offer regarding early detection of new lesions, thereby possibly limiting radiation exposure, and improving quality of life. The aim is to determine which biomarkers might be available in VHL patients and to assess their clinical relevance in diagnosing panNENs in VHL patients. We searched the databases of PubMed/Medline, Embase, and Web of Science to identify relevant articles. Seven studies assessing the diagnostic or prognostic value of biomarkers were included. The results from these studies were conflicting. Since no evident association between VHL-related panNENs and biomarkers was established in studies with larger study populations, currently biomarkers do not play a significant role in early detection or follow-up for panNENs in VHL patients. The absence of evidence underscores the need for specific research to address this unmet need