10 research outputs found
Building the analytical response in frequency domain of AC biased bolometers Application to Planck/HFI
Context: Bolometers are high sensitivity detector commonly used in Infrared
astronomy. The HFI instrument of the Planck satellite makes extensive use of
them, but after the satellite launch two electronic related problems revealed
critical. First an unexpected excess response of detectors at low optical
excitation frequency for {\nu} < 1 Hz, and secondly the Analog To digital
Converter (ADC) component had been insufficiently characterized on-ground.
These two problems require an exquisite knowledge of detector response. However
bolometers have highly nonlinear characteristics, coming from their electrical
and thermal coupling making them very difficult to modelize.
Goal: We present a method to build the analytical transfer function in
frequency domain which describe the voltage response of an Alternative Current
(AC) biased bolometer to optical excitation, based on the standard bolometer
model. This model is built using the setup of the Planck/HFI instrument and
offers the major improvement of being based on a physical model rather than the
currently in use had-hoc model based on Direct Current (DC) bolometer theory.
Method: The analytical transfer function expression will be presented in
matrix form. For this purpose, we build linearized versions of the bolometer
electro thermal equilibrium. And a custom description of signals in frequency
is used to solve the problem with linear algebra. The model performances is
validated using time domain simulations.
Results: The provided expression is suitable for calibration and data
process- ing. It can also be used to provide constraints for fitting optical
transfer function using real data from steady state electronic response and
optical response. The accurate description of electronic response can also be
used to improve the ADC nonlinearity correction for quickly varying optical
signals.Comment: 20 pages, 10 figure
Identification en thĂ©rapie cellulaire des patrons dâexpression transcriptomique de cellules souches utilisĂ©es pour traiter la dĂ©faillance cardiaque afin dâen amĂ©liorer le potentiel thĂ©rapeutique
La cardiopathie ischĂ©mique incluant lâinsuffisance cardiaque est la deuxiĂšme cause de mortalitĂ© annuelle au Canada. Bien que de nombreuses stratĂ©gies prĂ©ventives et des thĂ©rapies pharmacologiques retardent la progression de la maladie, il nâexiste aucune solution qui module directement aux les remaniements pathologiques et la perte de cardiomyocytes. Au cours des 25 derniĂšres annĂ©es, de multiples progrĂšs dans les domaines de la mĂ©decine rĂ©gĂ©nĂ©rative et de la thĂ©rapie cellulaire ont annoncĂ© des rĂ©sultats prometteurs, mais les rĂ©sultats dâĂ©tudes cliniques contemporaines demeurent plutĂŽt mitigĂ©s. COMPARE-AMI, une Ă©tude randomisĂ©e-contrĂŽlĂ©e de phase II, a Ă©valuĂ© lâeffet dâinjections intracoronariennes de cellules souches hĂ©matopoĂŻĂ©tiques CD133+ chez des patients souffrant dâinfarctus aigu. IMPACT-CABG, une ĂRC de phase II a Ă©galement Ă©valuĂ© lâeffet dâinjections intramyocardiques de cellules CD133+ chez les patients souffrant de cardiomyopathie ischĂ©mique chronique nĂ©cessitant une revascularisation chirurgicale. Nous avons Ă©mis lâhypothĂšse que les cellules CD133+ utilisĂ©es dans des Ă©tudes cliniques de cardiomyopathies ischĂ©miques aiguĂ«s et chroniques des patients rĂ©pondant Ă la thĂ©rapie cellulaire exhibent des signatures transcriptomiques communes responsables de leur effet thĂ©rapeutique. En classant les patients en tant que rĂ©pondants et non-rĂ©pondants selon leur fonction cardiaque, nous avons Ă©valuĂ©, a posteriori, ces patrons dâexpression. Les cellules CD133+ autologues de patients jugĂ©s rĂ©pondants expriment des signatures qui sont hautement conservĂ©es entre elles (incluant lâangiogĂ©nĂšse, la rĂ©gulation de la rĂ©ponse au stress et la survie cellulaire) et uniques dâun modĂšle Ă lâautre et qui pourraient, en partie, exprimer les issus cliniques des patients. Afin de maximiser les effets de la thĂ©rapie cellulaire aux cellules souches, nous avons par la suite tentĂ© de reproduire ces phĂ©notypes par stimulation pharmacologique avec des inhibiteurs dâHSP90 pour leurs effets qui semblent reproduire ces signatures. Ainsi, nous avons dĂ©montrĂ© quâune stimulation de cellules souches mĂ©senchymateuses humaines (CSMh) au CĂ©lastrol (inhibiteur HSP90) pouvait rĂ©pliquer certains de ces phĂ©notypes. Notamment, des CSMh conditionnĂ©es activent des voies de signalisation de type âRISKâ et augmentent leur sĂ©crĂ©tion de protĂ©ines en lien avec la rĂ©ponse au stress ainsi que dâexosomes contenant des molĂ©cules impliquĂ©es dans la communication intercellulaire sans ĂȘtre liĂ©es Ă un changement de type cellulaire. De plus, les CSMh traitĂ©es semblent amĂ©liorer la guĂ©rison de plaie par activitĂ© paracrine et sont plus rĂ©sistante Ă la sĂ©nescence oxydative. Ces rĂ©sultats encourageants nous permettent dâenvisager des stratĂ©gies plus poussĂ©es de prĂ©-conditionnement cellulaire ex vivo de cellules CD133+ avant leur implantation. Ă terme, cela pourrait mener Ă une optimisation de la thĂ©rapie cellulaire afin dâen maximiser les bĂ©nĂ©fices cliniques et dâen exploiter leur plein potentiel.Ischemic cardiomyopathy and heart failure are the second annual cause of mortality in Canada.
Despite rigorous prevention strategies and drug regimens preventing progression, no therapeutic
modality can currently reverse the pathologic modifications of the disease. In the last quarter century,
numerous advances in the field of regenerative medicine and cell therapy have demonstrated promising
effects. COMPARE-AMI, a phase II randomized controlled trial (RCT), evaluated the effect of intracoronary
injection of CD133+ cells in acute myocardial infarction following percutaneous intervention. IMPACTCABG,
also a phase II RCT, evaluated the effect of intramyocardial injection of CD133+ hematopoietic stem
cells in chronic ischemic cardiomyopathy at the time of surgical revascularization. That being said, we
believe that the CD133+ cells used in therapy have shared transcriptomic signatures that are responsible
for their clinical effects. By classifying patients into responders and non-responders according to an
improvement in ejection fraction, we evaluated, a posteriori, those expression patterns. Autologous
CD133+ cells of patients classified as responders expressed highly conserved transcriptomic signatures
that could be responsible for the clinical outcomes of patients. Notably, these signatures were related to
cardioprotective mechanisms including angiogenesis, stress response regulation and cell survival. In order
to harness the full potential of stem cell therapy, we attempted to reproduce the identified phenotypes
by pharmacological intervention with HSP90 inhibitors which are known to mimic some of these effets.
Conditioned human mesenchymal stem cells (hMSC) activated âRISKâ-type signaling pathways and
augmented exosome and protein secretion relating to the response to cellular stress; this activation was
unrelated to a switch of cell type. Furthermore, treated hMSC seemed to favour improved wound healing
by paracrine activity and were more resistant to oxidative senescence. These encouraging results allow
us to develop novel, more advance, strategies of ex vivo cell preconditioning before implantation,
including of CD133+ cells. Ultimately, we hope that optimisation of cells through this mechanism and
others will allow us to unleash the full potential of stem cell therapy
Recommended from our members
Catalase Prevents Maternal DiabetesâInduced Perinatal Programming via the Nrf2âHO-1 Defense System
We investigated whether overexpression of catalase (CAT) in renal proximal tubular cells (RPTCs) could prevent the programming of hypertension and kidney disease in the offspring of dams with maternal diabetes. Male offspring of nondiabetic and diabetic dams from two transgenic (Tg) lines (Hoxb7-green fluorescent protein [GFP]-Tg [controls] and Hoxb7/CAT-GFP-Tg, which overexpress CAT in RPTCs) were studied from the prenatal period into adulthood. Nephrogenesis, systolic blood pressure, renal hyperfiltration, kidney injury, and reactive oxygen species (ROS) generation were assessed. Gene expression of transforming growth factor-ÎČ1 (TGF-ÎČ1), nuclear factor erythroid 2p45ârelated factor-2 (Nrf2), and heme oxygenase-1 (HO-1) was tested in both in vitro and in vivo studies. Renal dysmorphogenesis was observed in offspring of Hoxb7-GFP-Tg dams with severe maternal diabetes; the affected male offspring displayed higher renal ROS generation and developed hypertension and renal hyperfiltration as well as renal injury with heightened TGF-ÎČ1 expression in adulthood. These changes were ameliorated in male offspring of diabetic Hoxb7/CAT-GFP-Tg dams via the Nrf2âHO-1 defense system. CAT promoted Nrf2 nuclear translocation and HO-1 gene expression, seen in both in vitro and in vivo studies. In conclusion, CAT overexpression in the RPTCs ameliorated maternal diabetesâinduced perinatal programming, mediated, at least in part, by triggering the Nrf2âHO-1 defense system
VITAE : VIrTual brAin pErfusion
VITAE is an ERC funded software project aimed at providing full brain simulations of cerebral blood flow and solute exchange between blood and the neural tissue. The endgoal is to understand fine scale interactions between the architecture of the microvascular network in the brain and its functions (blood supply, oxygen and nutrient delivery, waste removal). This may indeed help unveil potential causes of cerebral disease like Alzheimerâs Disease.
In the actual state of the art, full scale brain simulations are something new. First, acquiring input anatomical data of the blood vessel network is difficult and is an active domain of research. Next, simulation by itself is a CPU intensive Computational Fluid Dynamic problem requiring both inversion of large matrices and manipulation of large amounts of data.
The current milestone is capable of running pressure resolution in a full mouse brain composed of about 5 millions of microvessels in one second on 1024 processor cores. The software written in C++ fully supports parallelized IO and graph partitioning to optimize the placement of vertices and reduce computing times. The next challenge is to run simulations taking the complex behavior of blood into account, which requires to run the pressure solver from one hundred to several thousand times. This will require to improve significantly the convergence time.
Acknowledgements: ERC Funded Project: Proof of Concept (PoC), ERC-2018-PoC
A. Sauvé, J.-D. Julien, M. Berg, M. Peyrounette, P. Elyakime, Y. Davit, M. Pigou, S. Lorthoi
Fake news : les bibliothĂ©caires du QuĂ©bec veulent faire partie de lâĂ©quation
Cette enquĂȘte prĂ©sente les perceptions de la communautĂ© des bibliothĂ©caires professionnels du QuĂ©bec face aux fake news et les initiatives que celle-ci a mis en place pour lutter contre la dĂ©sinformation. Au moyen dâun questionnaire en ligne soumis durant lâĂ©tĂ© 2020, auquel 263 bibliothĂ©caires provenant de divers milieux dâexercice ont rĂ©pondu, la consultation rĂ©vĂšle que la quasi-totalitĂ© des bibliothĂ©caires se prĂ©occupent de la prĂ©sence des fake news dans le paysage mĂ©diatique. Pour freiner les fake news, lâĂ©ducation aux mĂ©dias et Ă lâinformation est une solution qui fait consensus, mais le recours aux lois reçoit un accueil mitigĂ©. GrĂące aux commentaires reçus, lâenquĂȘte met en lumiĂšre de nombreuses initiatives locales et nationales visant Ă sensibiliser la population aux dangers de la dĂ©sinformation et lui donner des outils pour Ă©viter de tomber dans le panneau. En conclusion, les auteurs encouragent les bibliothĂ©caires Ă intervenir davantage sur la place publique, notamment en faisant valoir leur expertise, car celle-ci peut ĂȘtre utile Ă lâensemble des citoyens.This survey presents the perceptions of the Quebec professional library community regarding fake news and the initiatives it has put in place to combat misinformation. Through an online questionnaire submitted in the summer of 2020, to which 263 librarians from various practice settings responded, the consultation reveals that almost all librarians are concerned about the presence of fake news in the media landscape. There is a consensus that educating citizens in information literacy skills is one way to stop fake news, but the use of legislative change receives a mixed reception. Thanks to the comments received, the survey highlights numerous local and national initiatives aiming to raise awareness of the dangers of misinformation and give people tools to avoid falling into the trap. In conclusion, the authors encourage librarians to become more involved in the public arena because their expertise can be useful to all citizens