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Characterization of TCDD-inducible poly-ADP-ribose polymerase (TIPARP/ARTD14) catalytic activity
YesHere, we report the biochemical characterization of the mono-ADP-ribosyltransferase 2,3,7,8-tetrachlorodibenzo-p-dioxin poly-ADP-ribose polymerase (TIPARP/ARTD14/PARP7), which is known to repress aryl hydrocarbon receptor (AHR)-dependent transcription. We found that the nuclear localization of TIPARP was dependent on a short N-terminal sequence and its zinc finger domain. Deletion and in vitro ADP-ribosylation studies identified amino acids 400–657 as the minimum catalytically active region, which retained its ability to mono-ADP-ribosylate AHR. However, the ability of TIPARP to ADP-ribosylate and repress AHR in cells was dependent on both its catalytic activity and zinc finger domain. The catalytic activity of TIPARP was resistant to meta-iodobenzylguanidine but sensitive to iodoacetamide and hydroxylamine, implicating cysteines and acidic side chains as ADP-ribosylated target residues. Mass spectrometry identified multiple ADP-ribosylated peptides in TIPARP and AHR. Electron transfer dissociation analysis of the TIPARP peptide 33ITPLKTCFK41 revealed cysteine 39 as a site for mono-ADP-ribosylation. Mutation of cysteine 39 to alanine resulted in a small, but significant, reduction in TIPARP autoribosylation activity, suggesting that additional amino acid residues are modified, but loss of cysteine 39 did not prevent its ability to repress AHR. Our findings characterize the subcellular localization and mono-ADP-ribosyltransferase activity of TIPARP, identify cysteine as a mono-ADP-ribosylated residue targeted by this enzyme, and confirm the TIPARP-dependent mono-ADP-ribosylation of other protein targets, such as AHR.This work was supported by Canadian Institutes of Health Research (CIHR) operating grants [MOP-494265 and MOP-125919]; CIHR New Investigator Award; an Early Researcher Award from the Ontario Ministry of Innovation [ER10-07-028]; the Johan Throne Holst Foundation; Novo Nordic Foundation; and the Norwegian Cancer Society to J.M. This work was also funded by grants from the Johan Throne Holst Foundation; and the Novo Nordic Foundation to H.I.N
Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water
Investigation of the solar wind-Moon interaction onboard Chandrayaan-1 mission with the SARA experiment
The SARA instrument (Sub-keV Atom Reflecting Analyser) comprises a low energy neutral atom (LENA) sensor for the energy range 10 eV-3.3 keV and an ion mass spectrometer (10 eV-15 keV). It is the first ever experiment to study the solar wind-planetary surface interaction via measurements of the sputtered atoms and neutralized back-scattered solar wind hydrogen. The neutral atom sensor uses conversion of the incoming neutrals to positive ions, which are then analysed via surface interaction technique. The ion mass spectrometer is based on the same principle. SARA performs LENA imaging of the Moon's elemental surface composition including that of permanently shadowed areas, and imaging of the lunar surface magnetic anomalies. It will also investigate processes of space weathering and sputtered sources of the exospheric gases