Special Issue 01

ABSTRAC

Estrogen treatment decreases matrix metalloproteinase (MMP)-9 in autoimmune demyelinating disease through estrogen receptor alpha (ERalpha).

Matrix metalloproteinases (MMPs) have a crucial function in migration of inflammatory cells into the central nervous system (CNS). Levels of MMP-9 are elevated in multiple sclerosis (MS) and predict the occurrence of new active lesions on magnetic resonance imaging (MRI). This translational study aims to determine whether in vivo treatment with the pregnancy hormone estriol affects MMP-9 levels from immune cells in patients with MS and mice with experimental autoimmune encephalomyelitis (EAE). Peripheral blood mononuclear cells (PBMCs) collected from three female MS patients treated with estriol and splenocytes from EAE mice treated with estriol, estrogen receptor (ER) alpha ligand, ERbeta ligand or vehicle were stimulated ex vivo and analyzed for levels of MMP-9. Markers of CNS infiltration were assessed using MRI in patients and immunohistochemistry in mice. Supernatants from PBMCs obtained during estriol treatment in female MS patients showed significantly decreased MMP-9 compared with pretreatment. Decreases in MMP-9 coincided with a decrease in enhancing lesion volume on MRI. Estriol treatment of mice with EAE reduced MMP-9 in supernatants from autoantigen-stimulated splenocytes, coinciding with decreased CNS infiltration by T cells and monocytes. Experiments with selective ER ligands showed that this effect was mediated through ERalpha. In conclusion, estriol acting through ERalpha to reduce MMP-9 from immune cells is one mechanism potentially underlying the estriol-mediated reduction in enhancing lesions in MS and inflammatory lesions in EAE

Load Balancing Techniques for Efficient Traffic Management in Cloud Environment

Cloud computing is an internet based computing. This computing paradigm has enhanced the use of network where the capability of one node can be utilized by other node. Cloud service provides access on demand to distributive resources such as database, servers, software, infrastructure etc. in pay as you go basis. Load balancing is one of the vexing issues in distributed environment. Resources of service provider need to balance the load of client request. Load balancing is adapted in order to increase the resource consumption in Data centers that leads to enhance the overall performance of system achieving client satisfaction

Thermal Stability and Antibacterial Activity of a Newly Prepared Organic Coating from a De-saturated Seed Oil of Thevetia Peruviana

Air drying polyesteramide-urethanes resins were synthesized from de-saturated fatty methyl ester (FAME) of Thevetia peruviana seed oil commonly known as Yellow oleanda (a tropical ornamental shrub) having about 68.8% unsaturated and 30.9% saturated fatty acids. Urea fractionation method was used to optimize the percentage of functional groups (in this case double bonds) by physio-sorption of the saturated fatty acids. The physico-chemical characterization of the optimized unsaturated FAME such as hydroxyl value, iodine value, saponification value, refractive index, inherent viscosity were carried out using standard methods. Through condensation reaction the N,N- bis(2-hydroxyethyl) Thevetia peruviana (HETA) prepared from the FAME react with isophthalic acid to form polyesteramide. The latter undergo urethanation with H1 • 2 MDI. The structural elucidation of the moisture cured polyesteramide-urethane urethane coating was based on FTIR, 1H-NMR and 13C-NMR spectroscopic methods. The coatings films were evaluated for its antibacterial activity and thermal stability properties on TGA and DSC. SEM of the coating films was also examine

Regulation of Stat5 by FAK and PAK1 in Oncogenic FLT3 and KIT driven Leukemogenesis

Oncogenic mutations of FLT3 and KIT receptors are associated with poor survival in patients with acute myeloid leukemia (AML) and myeloproliferative neoplasms (MPN) and currently available drugs are largely ineffective. Although Stat5 has been implicated in regulating several myeloid and lymphoid malignancies, how precisely Stat5 regulates leukemogenesis, including its nuclear translocation to induce gene transcription is poorly understood. In leukemic cells, we show constitutive activation of focal adhesion kinase (FAK), whose inhibition represses leukemogenesis. Downstream of FAK, activation of Rac1 is regulated by RacGEF Tiam1, whose inhibition prolongs the survival of leukemic mice. Inhibition of the Rac1 effector PAK1 prolongs the survival of leukemic mice in part by inhibiting the nuclear translocation of Stat5. These results reveal a leukemic pathway involving FAK/Tiam1/Rac1/PAK1 and demonstrate an essential role for these signaling molecules in regulating the nuclear translocation of Stat5 in leukemogenesis

An Android Application for Blind: Third Eye

With advances in new technologies, mobile devices have grown in popularity to become one of the most common consumer devices. Many of us can make a call or send a message at anytime from anywhere. As our society farther expands, there have been many supports for second-class citizens, disabled. There are a lot of visually impaired persons in and round the world. Physical disability of blind people makes their life difficult. They find themselveschallenging to meet daily challenges independently. One amongst several supports that are urgent is that the guarantee of quality for blind individuals.Several auxiliary tools are available to help blind people.But these could not able to help the user with basic mobile phone features. The main aim of this paper is to help the blind person to use basic features of smart phones by audible feedback and a single shake on this application identifies the location of place through GPS and sends a message comprising this location URL to the registered contacts and also make a call on the first registered contact to help the one in dangerous situations. Continuous location trailing info via SMS helps to seek out the situation of the victim quickly and might be saved safely

Short-Term History Based Authentication Using Smartphone Sensors and Apps

Secrete-QA could be a security primarily based application. A security question is asked once the user fails to login or forgets his/her password and to reset the password. A security question could be a secondary authentication, but these queries is guessed simply by an admirer or exposed to a strangers who might recognize our personal info like friends, relations or those that will access our personal info through public on-line tools. We will build these security queries additional customized by using the privilege of accessing our Smartphone sensors and apps while not affecting the user’s privacy. Three types of questions are generated that is, Yes/No questions, Multiple Choice questions and WH questions. An android app is created for the frequent updating of the Smartphone data and questions are asked in the web part using this updated data.The questions are generated by considering the legacy apps, GPS, calendar, app details etc.These have the mostmemorability to the users and high robustness to attacks

Class IA PI3Kinase Regulatory Subunit, p85α, Mediates Mast Cell Development through Regulation of Growth and Survival Related Genes

Stem cell factor (SCF) mediated KIT receptor activation plays a pivotal role in mast cell growth, maturation and survival. However, the signaling events downstream from KIT are poorly understood. Mast cells express multiple regulatory subunits of class 1A PI3Kinase (PI3K) including p85α, p85β, p50α, and p55α. While it is known that PI3K plays an essential role in mast cells; the precise mechanism by which these regulatory subunits impact specific mast cell functions including growth, survival and cycling are not known. We show that loss of p85α impairs the growth, survival and cycling of mast cell progenitors (MCp). To delineate the molecular mechanism (s) by which p85α regulates mast cell growth, survival and cycling, we performed microarray analyses to compare the gene expression profile of MCps derived from WT and p85α-deficient mice in response to SCF stimulation. We identified 151 unique genes exhibiting altered expression in p85α-deficient cells in response to SCF stimulation compared to WT cells. Functional categorization based on DAVID bioinformatics tool and Ingenuity Pathway Analysis (IPA) software relates the altered genes due to lack of p85α to transcription, cell cycle, cell survival, cell adhesion, cell differentiation, and signal transduction. Our results suggest that p85α is involved in mast cell development through regulation of expression of growth, survival and cell cycle related genes