Expeditious Calibration Method for Quantification of Odorous Mixtures via GC

An odour sample is generally a complex matrix consisting of numerous molecules (mostly volatile organic compounds, VOCs) chemically different from each other, and which can be perceived by the human nose. In order to chemically characterize this complex gaseous matrix, gas-chromatography (GC) is commonly used. By this analytical technique, it is possible to obtain the resolution of odour gaseous mixtures in order to qualify and quantify the compounds. However, the quantification is a tricky operation based on the comparison of the sample under examination with purpose-made gas mixtures containing a compound, chosen as a standard, at known concentration. Commonly, these mixtures are obtained from cylinders of compressed gases, the use of which involves problems relating to their management, thermodynamic equilibrium feasibility, considerable economic outlay and not-negligible procurement times. This paper describes a method, proposed as a versatile and simple alternative to the use of such cylinders, for preparing gaseous calibration standards at known concentration. The method involves the continuous injection of VOC in liquid form, by means of a syringe pump, into a stream of neutral gas, such as air or nitrogen, which acts as diluent gas, controlled by a mass flow meter. Exploiting the volatility of the compounds used, it is possible to generate a continuous gas stream, exiting the system, containing the selected VOC at the desired concentration, which can be directly used as calibration standard

Hydrolyzed protein based materials for biodegradable spray mulching coatings

Low density polyethylene (LDPE) films are widespread used in agriculture for soil mulching. The use of LDPE mulching films causes the serious drawback of huge quantities of waste to be disposed of. Over the last years the growing environmental awareness has been prompting the research to develop a new generation of mulching products starting with raw materials from renewable origin. These materials have to retain their physical and mechanical properties while in use and have to be compostable or biodegradable at the end of their life, degrading via micro-organisms into carbon dioxide or methane, water and biomass. The research is focused on the development of novel biodegradable polymeric materials based on hydrolyzed proteins, derived from waste products of the leather industry. Biodegradable soil mulching coatings were realized with these biodegradable polymeric materials by means of spray techniques; the coatings were tested in a Ligustrum ovalifolium cultivation carried out inside a greenhouse. This paper describes the functionalities of the new bio-based mulching coatings, which were developed and tested in real scale greenhouse cultivation tests. During the trial, the biodegradable soil mulching materials showed suitable properties for an efficient and profitable use in agriculture. The innovative biodegradable spray coatings lasted up to 18 months. The biodegradable materials could be the environmentally friendly alternatives to synthetic petro-chemical polymers and could contribute to a sustainable agriculture

the effects of control measures on the economic burden associated with epidemics of avian influenza in italy

In 1999, Italy experienced a devastating epidemic of high-pathogenicity avian influenza (HPAI) caused by an H7N1 virus subtype. After this epidemic, a ministerial decree was passed to implement control measures for low-pathogenicity avian influenza (LPAI) due to H5 and H7 subtypes. We investigated whether these control measures have decreased the public expenditure associated with epidemics of LPAI and HPAI by comparing the direct and consequential losses of the 1999 epidemic to the losses associated with successive epidemics. The estimated total economic burden of the epidemics was about euro650 million (euro217 million in direct losses and euro433 million in consequential losses). The 1999 epidemic accounted for most of these losses (euro507 million: euro112 million in direct losses and euro395 million in consequential losses), whereas the total economic burden for the 5 successive LPAI was euro143 million (euro105 million in direct losses and euro38 million in consequential losses). These results demonstrate that the implementation of a coordinated set of disease-control measures, which included both emergency and prophylactic vaccination, was able to reduce the overall costs associated with avian influenza epidemics. The results also show that the application of adequate LPAI control measures may limit the risk of emergence of an HPAI virus in an area with a high poultry density, allowing the complete disruption of the poultry market and its huge associated costs to be avoided

The birthplace and age of the isolated neutron star RX J1856.5-3754

X-ray observations unveiled various types of radio-silent Isolated Neutron Stars (INSs), phenomenologically very diverse, e.g. the Myr old X-ray Dim INS (XDINSs) and the kyr old magnetars. Although their phenomenology is much diverse, the similar periods (P=2--10 s) and magnetic fields (~10^{14} G) suggest that XDINSs are evolved magnetars, possibly born from similar populations of supermassive stars. One way to test this hypothesis is to identify their parental star clusters by extrapolating backward the neutron star velocity vector in the Galactic potential. By using the information on the age and space velocity of the XDINS RX J1856.5-3754, we computed backwards its orbit in the Galactic potential and searched for its parental stellar cluster by means of a closest approach criterion. We found a very likely association with the Upper Scorpius OB association, for a neutron star age of 0.42+/-0.08 Myr, a radial velocity V_r^NS =67+/- 13$ km s^{-1}, and a present-time parallactic distance d_\pi^NS = 123^{+11}_{-15} pc. Our result confirms that the "true" neutron star age is much lower than the spin-down age (tau_{sd}=3.8 Myrs), and is in good agreement with the cooling age, as computed within standard cooling scenarios. The mismatch between the spin-down and the dynamical/cooling age would require either an anomalously large breaking index (n~20) or a decaying magnetic field with initial value B_0 ~ 10^{14} G. Unfortunately, owing to the uncertainty on the age of the Upper Scorpius OB association and the masses of its members we cannot yet draw firm conclusions on the estimated mass of the RX J1856.5-3754 progenitor.Comment: 6 pages, accepted for publication on Monthly Notices of the Royal Astronomical Societ

Pasta consumption and connected dietary habits: Associations with glucose control, adiposity measures, and cardiovascular risk factors in people with type 2 diabetes—TOSCA.IT study

Background: Pasta is a refined carbohydrate with a low glycemic index. Whether pasta shares the metabolic advantages of other low glycemic index foods has not really been investigated. The aim of this study is to document, in people with type-2 diabetes, the consumption of pasta, the connected dietary habits, and the association with glucose control, measures of adiposity, and major cardiovascular risk factors. Methods: We studied 2562 participants. The dietary habits were assessed with the European Prospective Investigation into Cancer and Nutrition (EPIC) questionnaire. Sex-specific quartiles of pasta consumption were created in order to explore the study aims. Results: A higher pasta consumption was associated with a lower intake of proteins, total and saturated fat, cholesterol, added sugar, and fiber. Glucose control, body mass index, prevalence of obesity, and visceral obesity were not significantly different across the quartiles of pasta intake. No relation was found with LDL cholesterol and triglycerides, but there was an inverse relation with HDL-cholesterol. Systolic blood pressure increased with pasta consumption; but this relation was not confirmed after correction for confounders. Conclusions: In people with type-2 diabetes, the consumption of pasta, within the limits recommended for total carbohydrates intake, is not associated with worsening of glucose control, measures of adiposity, and major cardiovascular risk factors

New Young Star Candidates in BRC 27 and BRC 34

We used archival Spitzer Space Telescope mid-infrared data to search for young stellar objects (YSOs) in the immediate vicinity of two bright-rimmed clouds, BRC 27 (part of CMa R1) and BRC 34 (part of the IC 1396 complex). These regions both appear to be actively forming young stars, perhaps triggered by the proximate OB stars. In BRC 27, we find clear infrared excesses around 22 of the 26 YSOs or YSO candidates identified in the literature, and identify 16 new YSO candidates that appear to have IR excesses. In BRC 34, the one literature-identified YSO has an IR excess, and we suggest 13 new YSO candidates in this region, including a new Class I object. Considering the entire ensemble, both BRCs are likely of comparable ages, within the uncertainties of small number statistics and without spectroscopy to confirm or refute the YSO candidates. Similarly, no clear conclusions can yet be drawn about any possible age gradients that may be present across the BRCs.Comment: 54 pages, 19 figures, accepted by A

Autoantibodies Against Oxidized LDLs and Atherosclerosis in Type 2 Diabetes

OBJECTIVE —The aim of our study was to examine, in type 2 diabetic patients, the relationship between autoantibodies against oxidatively modified LDL (oxLDL Ab) and two indexes of atherosclerosis, intimal-medial thickness of the common carotid artery (CCA-IMT), which reflects early atherosclerosis, and the ankle-brachial index (ABI), which reflects advanced atherosclerosis. RESEARCH DESIGN AND METHODS —Thirty newly diagnosed type 2 diabetic patients, 30 type 2 diabetic patients with long duration of disease, and 56 control subjects were studied. To detect oxLDL Ab, the ImmunoLisa Anti-oxLDL Antibody ELISA was used. ABI was estimated at rest by strain-gauge plethysmography. Carotid B-mode imaging was performed on a high-resolution imaging system (ATL HDI 5000). RESULTS —In patients with long duration of disease, IgG oxLDL Ab were significantly higher and ABI significantly lower compared with the other two groups. We found a correlation between IgG oxLDL Ab and CCA-IMT in all diabetic patients. A significant inverse correlation between IgG oxLDL Ab and ABI only in patients with long duration of disease was seen, demonstrating a close relationship between these autoantibodies and advanced atherosclerosis. CONCLUSIONS —IgG OxLDL Ab may be markers of the advanced phase of the atherosclerotic process and the response of the immunological system to the oxLDL, which are present within atherosclerotic lesions

ASPETTI DELLA PRODUZIONE DEI PICCOLI RUMINANTI CON IMPATTO SULLA SALUTE UMANA

Negli ultimi anni l’attenzione del consumatore si è sempre più orientata verso le caratteristiche nutrizionali degli alimenti. Queste proprietà sono di grande importanza anche per quanto riguarda le produzioni dei piccoli ruminanti. Il presente lavoro ha lo scopo di riassumere i principali risultati emersi dal progetto di ricerca “Aspetti della produzione dei piccoli ruminanti con particolare impatto sulla salute umana”. Sono stati analizzati mediante i metodi descritti in letteratura: 1) i polimorfismi genetici dei biopeptidi del latte dei piccoli ruminanti; 2) le attività di alcuni enzimi della membrana del globulo di grasso e la frazione lipidica del latte ovino; 3) la qualità nutrizionale del latte e del formaggio ovino in relazione all’intensità di pascolamento; 4) le componenti bioattive di siero e scotta residui alla produzione dei formaggi ovi-caprini; 5) la resistenza genetica alle encefalopatie spongiformi trasmissibili e l’efficienza economica e biologica in razze ovine. I risultati ottenuti evidenziano, da svariati punti di vista, numerose potenzialità legate alle produzioni dei piccoli ruminanti e alle loro ricadute sulla salute umana