Social Emotional Learning Skill Development And Application

This capstone explores the development and application of social emotional learning skills in elementary age students. The research question addressed was “what social emotional learning skills are most important in a social emotional learning curriculum for elementary age students? Research surrounding the early development of these skills and the impact that social emotional learning has on overall academic success in the classroom is discussed. Based on findings in the research, a K-2 social emotional learning curriculum was created. The curriculum focuses on three themes- respect, social awareness and self-awareness. This curriculum aims to provide the most comprehensive and developmentally appropriate program for kindergarten-second grade students. The learning opportunities are hands on and engaging, through the incorporation of movement, technology, children’s literature, and real-world application. Additionally, the curriculum includes a home component and opportunities for students to develop goals throughout the course of the lesson

Interaction between Staphylococcus aureus and Pseudomonas aeruginosa is beneficial for colonisation and pathogenicity in a mixed-biofilm

Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early (<24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P<0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive phenotype in S. aureus. We show that co-infected keratinocytes exhibit an intermediate inflammatory response concurrent with impaired wound closure that is in keeping with a sustained pro-inflammatory response which allows for persistent microbial colonisation. These studies demonstrate that, contrary to the dominant hypothesis, interactions between S. aureus and P. aeruginosa may be an important factor for both colonisation and pathogenicity in the chronic infected wound

Ocean Futures Under Ocean Acidification, Marine Protection, and Changing Fishing Pressures Explored Using a Worldwide Suite of Ecosystem Models

Ecosystem-based management (EBM) of the ocean considers all impacts on and uses of marine and coastal systems. In recent years, there has been a heightened interest in EBM tools that allow testing of alternative management options and help identify tradeoffs among human uses. End-to-end ecosystem modeling frameworks that consider a wide range of management options are a means to provide integrated solutions to the complex ocean management problems encountered in EBM. Here, we leverage the global advances in ecosystem modeling to explore common opportunities and challenges for ecosystem-based management, including changes in ocean acidification, spatial management, and fishing pressure across eight Atlantis (atlantis.cmar.csiro.au) end-to-end ecosystem models. These models represent marine ecosystems from the tropics to the arctic, varying in size, ecology, and management regimes, using a three-dimensional, spatially-explicit structure parametrized for each system. Results suggest stronger impacts from ocean acidification and marine protected areas than from altering fishing pressure, both in terms of guild-level (i.e., aggregations of similar species or groups) biomass and in terms of indicators of ecological and fishery structure. Effects of ocean acidification were typically negative (reducing biomass), while marine protected areas led to both “winners” and “losers” at the level of particular species (or functional groups). Changing fishing pressure (doubling or halving) had smaller effects on the species guilds or ecosystem indicators than either ocean acidification or marine protected areas. Compensatory effects within guilds led to weaker average effects at the guild level than the species or group level. The impacts and tradeoffs implied by these future scenarios are highly relevant as ocean governance shifts focus from single-sector objectives (e.g., sustainable levels of individual fished stocks) to taking into account competing industrial sectors\u27 objectives (e.g., simultaneous spatial management of energy, shipping, and fishing) while at the same time grappling with compounded impacts of global climate change (e.g., ocean acidification and warming)

The intronic BRCA1 c.5407-25T>A variant causing partly skipping of exon 23—a likely pathogenic variant with reduced penetrance?

Rare sequence variants in the non-coding part of the BRCA genes are often reported as variants of uncertain significance (VUS), which leave patients and doctors in a challenging position. The aim of this study was to determine the pathogenicity of the BRCA1 c.5407-25T>A variant found in 20 families from Norway, France and United States with suspected hereditary breast and ovarian cancer. This was done by combining clinical and family information with allele frequency data, and assessment of the variant’s effect on mRNA splicing. Mean age at breast (n = 12) and ovarian (n = 11) cancer diagnosis in female carriers was 49.9 and 60.4 years, respectively. The mean Manchester score in the 20 families was 16.4. The allele frequency of BRCA1 c.5407-25T>A was 1/64,566 in non-Finnish Europeans (gnomAD database v2.1.1). We found the variant in 1/400 anonymous Norwegian blood donors and 0/784 in-house exomes. Sequencing of patient-derived cDNA from blood, normal breast and ovarian tissue showed that BRCA1 c.5407-25T>A leads to skipping of exon 23, resulting in frameshift and protein truncation: p.(Gly1803GlnfsTer11). Western blot analysis of transiently expressed BRCA1 proteins in HeLa cells showed a reduced amount of the truncated protein compared with wild type. Noteworthily, we found that a small amount of full-length transcript was also generated from the c.5407-25T>A allele, potentially explaining the intermediate cancer burden in families carrying this variant. In summary, our results show that BRCA1 c.5407-25T>A leads to partial skipping of exon 23, and could represent a likely pathogenic variant with reduced penetrance.publishedVersio

Monoskop Exhibition Library

The Exhibition Library reimagines the medium of art exhibition as well as that of art catalogue. Catalogues carry exhibitions through time and space, figuring as tropes for imagining arrangements and the course of works and settings they describe. However, they rarely give us a clue about what really happened, since they are often made before the show opens. Rather than documenting it, they often stand on their own, almost as if another work on display, truly as an artistic medium on its own. For this work, artists, designers, curators, poets and collectives created thirty catalogues of imaginary exhibitions. Exploring both the potential and impossible in art, the resulting exhibition library also serves as a “library of exhibitions.

Functional analysis of Casein Kinase 1 in a minimal circadian system

The Earth's rotation has driven the evolution of cellular circadian clocks to facilitate anticipation of the solar cycle. Some evidence for timekeeping mechanism conserved from early unicellular life through to modern organisms was recently identified, but the components of this oscillator are currently unknown. Although very few clock components appear to be shared across higher species, Casein Kinase 1 (CK1) is known to affect timekeeping across metazoans and fungi, but has not previously been implicated in the circadian clock in the plant kingdom. We now show that modulation of CK1 function lengthens circadian rhythms in Ostreococcustauri, a unicellular marine algal species at the base of the green lineage, separated from humans by ~1.5 billion years of evolution. CK1 contributes to timekeeping in a phase-dependent manner, indicating clock-mediated gating of CK1 activity. Label-free proteomic analyses upon overexpression as well as inhibition revealed CK1-responsive phosphorylation events on a set of target proteins, including highly conserved potentially clock-relevant cellular regulator proteins. These results have major implications for our understanding of cellular timekeeping and can inform future studies in any circadian organism

Smartphone Restriction and its Effect on Subjective Withdrawal Related Scores

Smartphone overuse is associated with a number of negative consequences for the individual and the environment. In the right end of the distribution of smartphone usage, concepts such as smartphone addiction seem warranted. An area that so far lacks research concerns the effect of smartphone restriction generally and specifically on subjective withdrawal related scores across different degrees of smartphone usage. The present study examined withdrawal related scores on the Smartphone Withdrawal Scale (SWS), the Fear of Mission Out Scale (FoMOs) and the Positive and Negative Affect Schedule (PANAS) scale during a smartphone restriction period, lasting 72 hours. In all, 127 participants were randomly assigned into one of two conditions; a restricted condition (n= 67) or a control condition (n= 60). During the restriction period, the aforementioned scales were completed three times a day by the participants. The results revealed a significant difference in scores between the restricted condition and the control condition on the SWS. Further, the participants with higher scores on smartphone addiction in the restricted condition were, compared to those with lower scores on smartphone addiction, significantly more negatively affected by the smartphone restriction condition, according to the SWS and the PANAS (Negative Affect) than those with higher and lower addiction scores in the control condition. This indicates that being restricted from one’s smartphone could cause significant withdrawal symptoms for an individual and that this effect is stronger among individuals with higher levels of smartphone addiction

Interleukin-6 as a marker of Huntington's disease progression: Systematic review and meta-analysis

Huntington's disease (HD) is a rare, inherited disorder with a broad spectrum of manifestations that vary with disease severity and progression. Although genetic testing can readily confirm the initial diagnosis of HD, markers sensitive to HD progression are needed to aid the development of individual treatment plans. The current analysis aims to identify plasma Interleukin-6 (IL-6) as a marker of disease progression in HD patients. A systematic search of PubMed and Medline from conception through October 2021 was conducted. Studies reporting plasma IL-6 levels of mutation-positive HD patients and healthy controls that met inclusion criteria were selected. The search strategy collected 303 studies, 9 of which met analysis inclusion criteria. From included studies, plasma IL-6 levels of 469 individuals with the HD mutation and 206 healthy controls were collected. Plasma IL-6 levels were meta-analytically compared between healthy controls and individuals with the confirmed HD mutation at all stages of disease and correlated to performance on standardized measures of total cognitive and motor function. Plasma IL-6 was significantly increased in HD groups compared to controls (g = 0.73, 95% CI = 0.31,1.16, P < 0.01) and increased significantly throughout most stages of disease progression, notably between pre-manifest and manifest (g = 0.31, 95% CI = 0.04,0.59, P < 0.05) and early and moderate HD stages (g = 0.52, 95% CI = 0.18,0.86, P < 0.01). Significant correlations between plasma IL-6 levels and HD symptomatic progression were identified, with increased cytokine levels associated with more severe motor impairments (r = 0.179, 95% CI = 0.0479,0.304, P = 0.008) and more extreme disabilities in activities of daily living and/or work tasks (r = −0.229, 95% CI = −0.334, −0.119, P < 0.001). Conclusively, plasma IL-6 levels correlate with disease and motor symptom progression and may act as a viable marker for clinical use. Analysis is limited by small study numbers and highlights the need for future work to identify definitive ranges or rates of change of plasma IL-6 levels that correlate to progressive HD disease states

Smartphone restriction and its effect on subjective withdrawal related scores

Excessive smartphone use has been associated with a number of negative consequences for the individual and the environment. Some similarities can be observed between excessive smartphone usage and several behavioural addictions, and continual usage constitutes one of several characteristics included in addiction. In the extreme high end of the distribution of smartphone usage, smartphone restriction might be expected to elicit negative effects for individuals. These negative effects may be regarded as withdrawal symptoms traditionally associated with substance-related addictions. To address this timely issue, the present study examined scores on the Smartphone Withdrawal Scale (SWS), the Fear of Missing Out Scale (FoMOS) and the Positive and Negative Affect Schedule (PANAS) during 72 h of smartphone restriction. A sample of 127 participants (72.4% women), aged 18–48 years (M = 25.0, SD = 4.5), were randomly assigned into one of two conditions: a restricted condition (experimental group, n = 67) or a control condition (control group, n = 60). During the restriction period participants completed the aforementioned scales three times a day. The results revealed significantly higher scores on the SWS and FoMOS for participants allocated to the restricted condition than those assigned to the control condition. Overall the results suggest that smartphone restriction could cause withdrawal symptoms