A comparative study of the effect of peeling and drying on phytochemical and proximate composition of ginger varieties in Nepal

The handling and processing of ginger are done by farmers in Nepal by following primitive practices that result in poor and unhygienically processed ginger of low quality. Due to little information on the quality and compositional aspects of ginger and its value-added product (essential oil), there is a need to improve traditional methods of processing and drying for a better quality of ginger and its product. This study aimed to assess the effects of peeling and drying conditions on two local ginger varieties in Nepal. A three-factor Completely Randomized Design (CRD) experiment was laid out at Ginger Research Program, Kapurkot, Salyan, Nepal. Three treatment factors were variety (Bose ginger and Nase ginger varieties), peeling (peeled and unpeeled ginger), and drying methods (direct sun drying and oven drying). After drying ginger rhizomes, the dry recovery percentage was calculated and the dried ginger rhizomes were ground to powder and subjected to laboratory analysis, where essential oil content and proximate composition of ginger powder were evaluated. Then, the extracted essential oil was subjected to GC-MS (Gas Chromatography and Mass Spectrometry) analysis to know the chemical composition of essential oil. The result obtained showed that unpeeled oven-dried gingers retained higher essential oil content (2 %). The moisture content of oven-dried peeled ginger was reduced to 10.49 % which is within the standard of 7-12 % acceptable to the international market unlike that of direct-sun drying which could only attain about 17% moisture content in the study area. Likewise higher dry recovery percentage (22.25%) was observed in unpeeled sun-dried gingers. Ether extract (5.05 %) and crude fiber (5.05 %) were higher in the Nase variety whereas nitrogen-free extract (75.51 %) was more efficient in Bose variety. From the GC-MS analysis of ginger oil, α-Zingiberene (16.61-21 %) was found to be a major chemical constituent of ginger essential oil followed by (E, E)-α-farnesene (8.68-10.99 %) and β-Sesquiphellandrene (8.26-10.23 %). The use of an oven to dry unpeeled ginger will improve the retention of essential oil; However, peeling of ginger showed reduced fiber content in the ginger

Effect of different processing methods on functional and physiochemical properties of turmeric (Curcuma longa Linn.) rhizome Var. Kapurkot Haledo-1

This study was conducted to evaluate the impact of different processing methods on the functional and phytochemical properties of turmeric rhizomes. The experiment consisted of four treatments: Treatment 1 involved oven-drying the turmeric powder, Treatment 2 involved blanching followed by oven-drying, Treatment 3 involved cooking followed by oven-drying, and Treatment 4 involved sun-drying. Each treatment was replicated four times. The major findings of the study revealed that sun-drying (68.50%) and blanching/oven-drying (66.50%) positively influenced the dispersibility of turmeric powder. Blanching/oven-drying (0.32 g/ml) and cooking/oven-drying (0.30 g/ml) significantly improved the bulk density of turmeric powder (p<0.001), with no significant difference observed between these two treatments. The water absorption capacity of the turmeric powders ranged from 3.35 to 5.35 g/ml, with the sun-dried sample displaying the lowest capacity and the cooked/oven-dried sample demonstrating the highest capacity. Similarly, sun-dried powder exhibited the lowest swelling power and solubility, while heat treatment resulted in a substantial increase in both of these parameters. Additionally, the curcumin content was found to be highest in the cooked/oven-dried (3.11%) and sun-dried (2.99%) turmeric powder. In conclusion, this study suggests that blanching and cooking methods have wide applicability in the food industry to enhance the bulkiness of turmeric powder for appropriate packaging and handling. Moreover, these methods contribute to the characteristic flavor and aroma of turmeric. The findings emphasize the importance of considering different processing techniques for optimizing the functional and phytochemical properties of turmeric, thus enabling its effective utilization in various food applications

Breeds and Breeding System of Indigenous and Crossbred Goats in Nepal

Goats are the indispensable component of rural economy in Nepal where 10.98 million goats accounting for 45.3% of total ruminants contribute to 20.3% of the total meat production and 49.2 million US dollar in the national economy. Being popular as “poor man’s cow” and “living cheque,” they significantly contribute to food, nutritional, and economic security of the marginalized farmers creating employment opportunities in the country. This chapter has tried to review the existing breeds, their breeding systems, challenges and way forward to enhance production and productivity of goats. Findings revealed that Nepal is endowed with four major indigenous genetic resources of goats and their crosses with Boer, Jamnapari, Barbari, Sirohi, etc. Occasionally, native goat breeds of Nepal were crossed with Kiko and Damascus as well. Breeding of goats in Nepal is mainly based on selection, pure breeding within indigenous flock, and crossbreeding to the available exotic breeds. There have been some biotechnological approaches applied in goat breeding and evaluation of native breeds. Estrus synchronization followed by artificial insemination is currently being practiced. Goats, being the most popular and easy source of household income and family nutrition in Nepal, could be the important source of national revenue provided with improved breeding and other husbandry practices

Selective Breeding to Improve Productive and Reproductive Performances and Survivability of Indigenous Sakini Chicken

Indigenous chickens are quite popular in Family Poultry Production System (FPPS) in Nepal, but are constrained with their low productive performance. The present study evaluated the productive and reproductive performance of Sakini chicken in different filial generations and sexes. In addition, research also aimed at understanding the effect of generations on above performances. Initially, base populations (G-0) of Sakini were maintained by collecting eight weeks old birds from different agro-ecologial zones of Nepal. Performance of the birds of G-0 was evaluated based on weekly body weight (12-24 weeks), laying performance, fertility, hatchability, hatch weight and survivability. Selected birds of base population (G-0) were used to produce first (G-1), second (G-2) and third (G-3) generations through selective breeding in each generation. Similarly, body weights at hatching, 12 weeks, 16 weeks, 20 week and 24 weeks were significantly (p<0.001) improved from G0 to G3 and were also significantly (p<0.001) differ for sex (males were always heavier than females). Likewise, there was significant (p<0.05) improvement in egg production (per hen per year), age at first lay (days), body weight at sexual maturity, egg number and egg weight at 90 days of laying in progressive generations. Fertility, hatchability and survivability significantly (p<0.05) improved in selected generations in comparison to base population, whereas, no significant difference was obtained within the different selected population. Thus, indigenous Sakini chicken under this experiment performed better with respect to survivability, fertility and hatchability in later generations that provides ample scope of advancing selective breeding activities within the indigenous population in order to bring significant improvement in the overall productive performance of Sakini chicken in Nepal

PCR Based Genotyping of Lulu Cattle of Nepal for A1, A2 Type Beta-caseins

Lulu is an indigenous breed of cattle (Bos taurus) found in high altitude regions of western Nepal. Population of Lulu cattle has been declining due to introgression with other exotic breeds to increase milk productivity. Here we aimed at finding potential approach for conserving Lulu cattle and its assets by studying the milk contents and investigating which variant of beta-casein protein is present in this breed. Beta caseins are an abundant protein in cow milk with A1 and A2 being the most common genetic variants of this protein. Consumption of A1 type of milk has numerous health-related complications whereas A2 type of milk has numerous human health promoting factors. We used restriction fragment length polymorphism (RFLP) for determining the A1 and A2 variant of beta casein in Lulu cattle. For performing DNA extraction, we collected (n = 18) blood samples of Lulu from Mustang and (n=17) Nepal Agriculture research council farm. The amplified fragments in 3% agarose at 251bp and 213bp respectively confirmed the presence of both A1 and A2 gene in Lulu; however, A2 was of greater abundance. Our study indicated that Lulu has A2 variant of beta-casein predominantly. The gene frequency of A1A1 is 0, A1A2 is 0.06 and A2A2 is 0.94. We further found that the allele frequency of A1 and A2 is 0.03 and 0.97 respectively. We designed special primer for sequencing CSN2 genes since A2 type beta casein gene was predominantly seen on Lulu. The sequencing result further supports our RFLP result as most of our samples have “C” nucleotide SNP in amplified CSN2 gene sequence. The Chi-square value of the current study is 0.04 which supports Hardy-Weinberg equilibrium inferring that Lulu cattle are still in the pure state, where there is no genetic introgression with the exotic breed for the sake of improvement of productivity

Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model

Background: In bone tissue engineering (BTE), extensive research into vascular endothelial growth factor A (VEGFA)-mediated angiogenesis has yielded inconsistent results. The aim of this study was to investigate the influence on angio- and osteogenesis of adenoviral-mediated delivery of VEGFA alone or in combination with bone morphogenetic protein 2 (BMP2) in bone marrow stromal cells (BMSC) seeded onto a recently developed poly(LLA-co-CL) scaffold. Methods: Human BMSC were engineered to express VEGFA alone or in combination with BMP2 and seeded onto poly(LLA-co-CL) scaffolds. Changes in angiogenic and osteogenic gene and protein levels were examined by quantitative reverse-transcription polymerase chain reaction (RT-PCR), PCR array, and alkaline phosphatase assay. An in vivo subcutaneous mouse model was used to investigate the effect on angio- and osteogenesis of VEGFA alone or in combination with BMP2, using microcomputed tomography (μCT), histology, immunohistochemistry, and immunofluorescence. Results: Combined delivery of a lower ratio (1:3) of VEGFA and BMP2 (ad-BMP2 + VEGFA) led to upregulation of osteogenic and angiogenic genes in vitro at 3 and 14 days, compared with mono-delivery of VEGFA (ad-VEGFA) and other controls. In vivo, in a subcutaneous mouse model, both ad-VEGFA and ad-BMP2 + VEGFA scaffold explants exhibited increased angiogenesis at 2 weeks. Enhanced angiogenesis was largely related to the recruitment and differentiation of mouse progenitor cells to the endothelial lineage and, to a lesser extent, to endothelial differentiation of the implanted BMSC. μCT and histological analyses revealed enhanced de novo bone formation only in the ad-BMP2 + VEGFA group, corresponding at the molecular level to the upregulation of genes related to osteogenesis, such as ALPL, RUNX2, and SPP1. Conclusions: Although BMSC expressing VEGFA alone or in combination with BMP2 significantly induced angiogenesis, VEGFA alone failed to demonstrate osteogenic activity both in vitro and in vivo. These results not only call into question the use of VEGFA alone in bone regeneration, but also highlight the importance in BTE of appropriately formulated combined delivery of VEGFA and BMP2.publishedVersio

Acid Peptic Disease among Patients with Acute Abdomen Visiting the Department of Emergency Medicine in a Tertiary Care Centre

Introduction: Acid peptic disease is caused by excessive acid secretion or weakened mucosal defense. Symptoms include epigastric pain, bloating, and nausea. Factors like gastric acid, Helicobacter pylori infection, alcohol consumption, smoking, and stress contribute to peptic ulcers. Imbalances between offensive and defensive factors can lead to ulcers. Acid-related disorders impact the quality of life and mortality. Accurate diagnosis and prompt treatment are vital. This study aimed to find out the prevalence of acid peptic disease among patients with acute abdomen in the Department of Emergency Medicine in a tertiary care centre. Methods: A descriptive cross-sectional study was conducted from 2 April 2022 and 2 April 2023 among the patients presented in the Department of Emergency Medicine in a tertiary care centre. Ethical approval was obtained from the Institutional Review Committee. All patients presenting with acute abdominal pain in the Emergency Department were included in the study. Patients not giving consent were excluded from the study. Convenience sampling method was used. The point estimate was calculated at a 95% Confidence Interval. Results: Out of the 400 patients with acute abdomen, the prevalence of acid peptic disease was found to be 87 (21.75%) (17.71-25.79, 95% Confidence Interval). Conclusions: The prevalence of acid peptic disease among patients with acute abdomen was found to be lower than in other studies performed in similar settings

MicroRNA-138 abates fibroblast motility with effect on invasion of adjacent cancer cells

Background: Recent studies have shown aberrant expression of micro-RNAs in cancer-associated fibroblasts (CAFs). This study aimed to investigate miR-138 dysregulation in CAFs in oral squamous cell carcinoma (OSCC) and its effects on their phenotype and invasion of adjacent OSCC cells. Methods: Expression of miR-138 was first investigated in OSCC lesions (n = 53) and OSCC-derived CAFs (n = 15). MiR-138 mimics and inhibitors were used to functionally investigate the role of miR-138 on CAF phenotype and the resulting change in their ability to support OSCC invasion. Results: Expression of miR-138 showed marked heterogeneity in both OSCC tissues and cultured fibroblasts. Ectopic miR-138 expression reduced fibroblasts’ motility and collagen contraction ability and suppressed invasion of suprajacent OSCC cells, while its inhibition resulted in the opposite outcome. Transcript and protein examination after modulation of miR-138 expression showed changes in CAF phenotype-specific molecules, focal adhesion kinase axis, and TGFβ1 signaling pathway. Conclusions: Despite its heterogeneous expression, miR-138 in OSCC-derived CAFs exhibits a tumor-suppressive function.publishedVersio

EFFICIENT RESOURCE MANAGEMENT ON EMBEDDED DEVICES VIA ISOLATION AND ADAPTIVE RESOURCE ALLOCATION

The advent of Chip Multiprocessor (CMP) with high performance, compact size and power efficiency has made many engineering marvel possible. CMPs has played great role in industrial automation, autonomous vehicle, embedded AI, and medical prognosis. In industrial autonomy or in autonomous vehicle there are many critical task which has to be run in isolation without any interference and delay. Virtualization software (Hypervisors) are being used for application isolation in CMPs. Hypervisors such as XEN, KVM are fully fledged hypervisor with many features and have their own scheduling scheme thus, scheduling overhead. In this thesis we used light-weight partitioning hypervisor known as Jailhouse in order to provide isolation to critical task. From our experiment we see that Jailhouse provide better isolation without any scheduling overhead which is suitable for real time application. As Jailhouse partition available resources among cells without any emulation, the number of cell we can create is limited. Also, the resources from root cell (which runs Linux) get divided and application running on it may suffer from resource constraints. We purpose adaptive offloading in order to address this issue which shows performance and quality improvement. We also explore deep learning and its implementation in edge computing device. The availability of GPUs and large data set made it possible to use deep learning state-of-art in many fields computer vision, medical diagnosis, image processing, surveillance, etc. It is evident that deep learning consists of two parts training and inferencing, both of these are power and compute intensive. We implemented YOLOV3 object detection state-of-art algorithm in NVIDIA AGX Xavier. We utilized Tensor and NVDLA cores in the Xavier using NVIDIA TensorRT and CUDA library. This has resulted more than 100% improvement in performance and significant decrease in power consumption from original YOLOV3 in FP16 precision. We explorer FP16 and INT8 precision with TensorRT, and DLA. INT8 precision further optimizes the performance and power with some compromise in accuracy. Our results shows, we can optimize inference engine by using TensorRT and DLA in edge computing device like Jetson Xavier

Overtopping and breaching of rockfill dams with and without a central core

Embankment dams are vulnerable to excessive through flow and overtopping due to erodible and pervious nature of materials used such as rockfill and earthfill. Majority of dams in the world are embankment type which pose serious risks and possible hazards in the downstream areas in case of failure. The seriousness of this topic has created a huge demand for dam safety and regulation. It is therefore very important to have a reliable consequence classification of dam. Implementation of the regulations and the proper design requirements demand full knowledge and understanding of dam behaviour during failure and in extreme load situation. So this study has become a step forward in the direction of defining and evaluating breach process and parameters. Eight different physical models are presented here which include dams with central core and without core. All of these are rockfill dams and subjected to excess through flow and overtopping, and the results are observed for both the types. It was noted that dams without central core see excess throughflow while dams with core failed mostly with overtopping. Unlike dams with additional protection layers, there is no sudden breakout and collapse giving huge downstream flood. However the peak discharge associated with the dam breaching is significant and should be addressed in the floodzone mapping. Some already existed parametric models are also employed to compare results with the physical modelling. Softwares like SFM (Agisoft), GIS, R programming script help to handle big data files and extract quality result from the analysis