Antioxidative effect of Arthrospira platensis biomass on the lipid oxidation

Introduction: Cyanobacteria are promising natural sources of antioxidants. Environmental conditions that influence synthesis of substances in cultures of cyanobacteria have been studied. Objective: This investigation aimed to evaluate the antioxidative effect of Arthrospira platensis biomass on the process of lipid oxidation in bulk oil and oil/water emulsion. Method: Arthrospira platensis growth potential for antioxidant production under different cultivation conditions using an experimental design. The antioxidative effect of the methanolic extracts and the biomass on preventing lipid oxidation was measured by peroxide value (PV). Results: The results showed that the growing conditions to obtain the biomass promoted change in the protective ability of different biomass extracts. Extracts obtained from the cultivated biomass grown under 150 μmol photons/m2s-1, 1.875 g.L-1 NaNO3; 13.5 g.L-1 NaHCO3 (assay14) and 50 μmol photons/m2s-1, 2.5 g.L-1 NaNO3; 18.0 g.L-1 NaHCO3 (standard)  showed the most antioxidative effect on preventing lipid oxidation, therefore used in the formulation of mayonnaise. The mayonnaise made with soybean oil and 0.5% biomass was preserved against lipid photodegradation during seven days of storage, but the peroxide value related to control varied from 2.9 to 3.1 mEqO2.Kg-1, with no significant difference in the preservation afforded by tert-butilhydroquinone, in the same period of storage. In mayonnaise made with sunflower oil, biomass, independent of concentration, was unable to protect the product against photooxidation. Conclusions: The results demonstrate the protective ability on preventing lipid oxidation of Arthrospira platensis biomass and its potential for use in food lipid-based of soybean oil.TÍTULO PT: Efeito antioxidante da biomassa de Arthrospira platensis sobre a oxidação lipídicaIntrodução: Cianobactérias são promissoras fontes naturais de antioxidantes. Condições ambientais que influenciam síntese de substâncias em cultivos de cianobactérias tem sido estudadas. Objetivo: O objetivo deste estudo foi avaliar o efeito antioxidante da biomassa de Arthrospira platensis no processo de oxidação lipídica do óleo e da emulsão óleo/água. Método: A produção de antioxidantes por Arthrospira platensis em função das condições de cultivo foi avaliada por meio de planejamento experimental. O efeito antioxidante dos extratos metanólicos e das biomassas foi avaliado através do índice de peróxidos. Resultados: Os resultados mostram que as condições de crescimento para se obter a biomassa promoveram mudança na capacidade protetora dos diferentes extratos da biomassa. Extratos obtidos a partir da biomassa cultivada sob 150 μmol fótons/m2s-1, 1,875 g.L-1 NaNO3; 13,5 g.L-1 NaHCO3 (ensaio 14) e 50 μmol fótons/m2s-1, 2,5 g.L-1 NaNO3; 18,0 g.L-1 NaHCO3 (padrão) mostraram maior efeito antioxidante contra a oxidação lipídica, portanto, utilizados para formulação da maionese. A maionese feita com óleo de soja e 0,5% de biomassa foi preservada da fotodegradação lipídica durante sete dias de armazenamento, mas o índice de peroxido em relação ao controle variou de 2,9 para 3,1 mEqO2.Kg-1, não havendo diferença significativa da preservação proporcionada pela terc-butil hidroquinona, no mesmo período de armazenamento. Na maionese feita com óleo de girassol, a biomassa, independentemente da concentração, não foi capaz de proteger o produto contra a foto-oxidação. Conclusões: Os resultados demonstram a capacidade protetora contra a oxidação lipídica da biomassa de Arthrospira platensis e seu potencial para uso em alimentos ricos em lipídeos à base de óleo de soja

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Analysis of Salmonella in free-range eggs through polymerase chain reaction

A identificação de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redução de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detecção da infecção, que, muitas vezes, cursa sem sinais clínicos. A Reação em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detecção de Salmonella, pois vários autores têm utilizado a PCR para verificar a presença da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipulação das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos foram divididos em grupos de seis, totalizando sessenta amostras. O exame bacteriológico foi realizado conforme metodologia preconizada pelas normas técnicas e a metodologia de extração de DNA pelo fenol-clorofórmio. A PCR foi realizada para a amplificação de um fragmento de DNA de 284 pb. A análise dos resultados não demonstrou diferença significativa entre a PCR e o bacteriológico. Todas as amostras positivas ao bacteriológico foram positivas na PCR, sendo que essa última detectou duas amostras a mais, devido a sua alta sensibilidade e especificidade, especialmente quando é sabido que os ovos apresentam uma população microbiana mista que, muitas vezes, impede o isolamento adequado das salmonelas no bacteriológico pela competição com a flora bacteriana normalmente presente.The identification of salmonella infection in commercial poultry has been one of the strong points of prophylaxis and consequent reduction of salmonellosis outbreaks in humans associated to consumption of eggs, considering that the analysis of the eggs can be one more point of detection of infection, which for many times appear without clinical signs. The Polymerase Chain Reaction (PCR) seems to be a useful strategy for Salmonella detection, because various authors have used the PCR to verify the presence of bacteria in meat, feces, tissues, blood, milk and eggs, with different methods of manipulation of samples. We have analyzed 360 eggs from ten farms, producers of free range-eggs, in the district of Camobi, in Santa Maria . RS . Brasil. The eggs were grouped in pools of six, totaling sixty samples The bacteriological exam was done in compliance with the method preconized by the technical rules and the method for extraction of DNA was by phenol-chloroform. The PCR was performed for the amplification of a 284 bp DNA fragment. The analysis of the results do not show significant difference between the PCR and the bacteriological exam. All positive samples in the bacteriological exam were also positive by PCR, however the PCR detected more two samples due to higher sensitivity and specificity, specially when it is known that the eggs show a mixed population of germs that many times difficult isolation of salmonellas in the bacteriological exam because of the competition with normal flora bacteria

Analysis of Salmonella in free-range eggs through polymerase chain reaction

A identificação de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redução de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detecção da infecção, que, muitas vezes, cursa sem sinais clínicos. A Reação em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detecção de Salmonella, pois vários autores têm utilizado a PCR para verificar a presença da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipulação das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos foram divididos em grupos de seis, totalizando sessenta amostras. O exame bacteriológico foi realizado conforme metodologia preconizada pelas normas técnicas e a metodologia de extração de DNA pelo fenol-clorofórmio. A PCR foi realizada para a amplificação de um fragmento de DNA de 284 pb. A análise dos resultados não demonstrou diferença significativa entre a PCR e o bacteriológico. Todas as amostras positivas ao bacteriológico foram positivas na PCR, sendo que essa última detectou duas amostras a mais, devido a sua alta sensibilidade e especificidade, especialmente quando é sabido que os ovos apresentam uma população microbiana mista que, muitas vezes, impede o isolamento adequado das salmonelas no bacteriológico pela competição com a flora bacteriana normalmente presente.The identification of salmonella infection in commercial poultry has been one of the strong points of prophylaxis and consequent reduction of salmonellosis outbreaks in humans associated to consumption of eggs, considering that the analysis of the eggs can be one more point of detection of infection, which for many times appear without clinical signs. The Polymerase Chain Reaction (PCR) seems to be a useful strategy for Salmonella detection, because various authors have used the PCR to verify the presence of bacteria in meat, feces, tissues, blood, milk and eggs, with different methods of manipulation of samples. We have analyzed 360 eggs from ten farms, producers of free range-eggs, in the district of Camobi, in Santa Maria . RS . Brasil. The eggs were grouped in pools of six, totaling sixty samples The bacteriological exam was done in compliance with the method preconized by the technical rules and the method for extraction of DNA was by phenol-chloroform. The PCR was performed for the amplification of a 284 bp DNA fragment. The analysis of the results do not show significant difference between the PCR and the bacteriological exam. All positive samples in the bacteriological exam were also positive by PCR, however the PCR detected more two samples due to higher sensitivity and specificity, specially when it is known that the eggs show a mixed population of germs that many times difficult isolation of salmonellas in the bacteriological exam because of the competition with normal flora bacteria

Resumos concluídos - Educação

Resumos concluídos - Educaçã

Análise da contaminação por Salmonella em ovos do tipo colonial através da reação em cadeia da polimerase Analysis of Salmonella in free-range eggs through polymerase chain reaction

A identificação de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redução de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detecção da infecção, que, muitas vezes, cursa sem sinais clínicos. A Reação em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detecção de Salmonella, pois vários autores têm utilizado a PCR para verificar a presença da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipulação das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos foram divididos em grupos de seis, totalizando sessenta amostras. O exame bacteriológico foi realizado conforme metodologia preconizada pelas normas técnicas e a metodologia de extração de DNA pelo fenol-clorofórmio. A PCR foi realizada para a amplificação de um fragmento de DNA de 284 pb. A análise dos resultados não demonstrou diferença significativa entre a PCR e o bacteriológico. Todas as amostras positivas ao bacteriológico foram positivas na PCR, sendo que essa última detectou duas amostras a mais, devido a sua alta sensibilidade e especificidade, especialmente quando é sabido que os ovos apresentam uma população microbiana mista que, muitas vezes, impede o isolamento adequado das salmonelas no bacteriológico pela competição com a flora bacteriana normalmente presente.<br>The identification of salmonella infection in commercial poultry has been one of the strong points of prophylaxis and consequent reduction of salmonellosis outbreaks in humans associated to consumption of eggs, considering that the analysis of the eggs can be one more point of detection of infection, which for many times appear without clinical signs. The Polymerase Chain Reaction (PCR) seems to be a useful strategy for Salmonella detection, because various authors have used the PCR to verify the presence of bacteria in meat, feces, tissues, blood, milk and eggs, with different methods of manipulation of samples. We have analyzed 360 eggs from ten farms, producers of free range-eggs, in the district of Camobi, in Santa Maria - RS - Brasil. The eggs were grouped in pools of six, totaling sixty samples. The bacteriological exam was done in compliance with the method preconized by the technical rules and the method for extraction of DNA was by phenol-chloroform. The PCR was performed for the amplification of a 284 bp DNA fragment. The analysis of the results do not show significant difference between the PCR and the bacteriological exam. All positive samples in the bacteriological exam were also positive by PCR, however the PCR detected more two samples due to higher sensitivity and specificity, specially when it is known that the eggs show a mixed population of germs that many times difficult isolation of salmonellas in the bacteriological exam because of the competition with normal flora bacteria

Gestão pública II: relatos, pesquisas, experiências e visão de trabalho.

Coletânea de trabalhos publicados e premiados no II Seminário de Gestão Pública da UFRA e na 1ª edição do Prêmio Novos Ventos de Gestão Pública, sob a responsabilidade da Pró-Reitoria de Gestão de Pessoas (PROGEP).UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIAEste livro, na forma de coletânea, se constituiu em um processo mais amplo de reflexão sobre a gestão pública dentro da Universidade. Aqui, partimos para uma importante reflexão, a partir do processo de sistematizar experiências, relatos de experiência, e pesquisas, sobre a Universidade Pública e sua relação com as pessoas e as ciências. Em especial, o capítulo 1, a partir da coletânea dos trabalhos apresentados no II Seminário de Gestão Pública, diferencia-se dos trabalhos submetidos para a primeira edição. As experiências destacadas para este livro reforçam a identidade e missão da UFRA, como os resultados dos trabalhos “OS CAMINHOS DA MERENDA ESCOLAR: análise da logística na cadeia de suprimentos do município de Tomé-Açu” e “ANÁLISE QVT NA GESTÃO DE UMA EMPRESA PRODUTORA DE DENDÊ EM TOMÉ-AÇU/PA”, e que também agregam valor a gestores e equipes de trabalho quando promovem a discussão de temas relacionados ao dia-a-dia do trabalho, tais como: QUALIDADE DE VIDA NO TRABALHO e GESTÃO ESTRATÉGICA DE PESSOAS NO SETOR PÚBLICO. Nesse sentido, servidores e pesquisadores cumprem a função de embasar inúmeras discussões que vêm sendo travadas pelas Instituições Públicas sobre qualidade de vida, inclusão, garantia de direitos, melhoria de procedimentos, dentre outros. O capítulo II, enquanto coletânea das experiências de trabalho indicada para premiação, é um momento riquíssimo de se perceber a quantidade e a qualidade de ações planejadas, organizadas e realizadas por servidores públicos. Em tempos que algumas práticas do serviço público não são bem vistas, reforça-se aqui que alguns dos relatos foram concebidos em meio a falta de recursos financeiros e estruturais, mas que não impediram os proponentes de seguir em frente. Mais do que isso, o reconhecimento dos beneficiários e usuários faz com que reforcemos a necessidade de fortalecer ações que agregam inúmeros valores a vida laboral dos servidores públicos e a própria Universidade Pública.This book, in the form of a collection, constituted a broader process of reflection on public management within the University. Here, we start an important reflection, based on the process of systematizing experiences, experience reports, and research, about the Public University and its relationship with people and the sciences. In particular, chapter 1, from the collection of works presented at the II Seminar on Public Management, differs from the works submitted for the first edition. The experiences highlighted for this book reinforce UFRA's identity and mission, such as the results of the work “THE PATHS OF SCHOOL BREAKFAST: analysis of logistics in the supply chain in the municipality of Tomé-Açu” and “QVT ANALYSIS IN THE MANAGEMENT OF A PRODUCING COMPANY OF DENDÊ IN TOMÉ-AÇU / PA ”, and which also add value to managers and work teams when promoting the discussion of topics related to day-to-day work, such as: QUALITY OF LIFE AT WORK and STRATEGIC PEOPLE MANAGEMENT IN THE PUBLIC SECTOR. In this sense, civil servants and researchers fulfill the function of supporting numerous discussions that have been held by Public Institutions on quality of life, inclusion, guarantee of rights, improvement of procedures, among others. Chapter II, as a collection of work experiences indicated for awards, is a very rich moment to realize the quantity and quality of actions planned, organized and carried out by public servants. In times when some public service practices are not well regarded, it is reinforced here that some of the reports were conceived amid a lack of financial and structural resources, but that did not prevent proponents from moving forward. More than that, the recognition of beneficiaries and users makes us reinforce the need to strengthen actions that add innumerable values ​​to the working life of public servants and the Public University itself

Núcleos de Ensino da Unesp: artigos 2014: volume 4: os processos de interação na escola e educação inclusiva

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Resumos concluídos - Ciências Biológicas

Resumos concluídos - Ciências Biológica