MORPHOLOGICAL, CONTROVERSIAL AND LITERARY REVIEW OF SHANKHPUSHPI

India is enriched in diversity of flora since ages. The ancient professionals have kept records of their work related to the plants and their works are a source of research today. Shankhpushpi is a well known herb since the most primitive works on Ayurveda have its therapeutic uses well elaborated and its morphological features well described. Later on, controversies erupt as locals in different parts of India used different plant species in the name of Shankhpushpi. Mainly four different plant species are considered in the name Shankhpushpi across India. These are Convolvulus pluricaulis, Clitorea ternatea, Evolvulus alsenoides and Canscora decussata. Shankhpushpi is a widely used herbal drug in the management of cognitive disorders from the times of Acharya Charak (200 BC) till today. Shankhpushpi has been categorized as a Medhya drug i.e. an efficient brain tonic by all ancient texts with no exceptions. Modern researches have established Shankhpushpi as an effective drug to be used either single or in combination, in the treatment of central nervous system disorders and boosting intellect. This article is an attempt to review the morphological features of the plant taken as Shankhpushpi along with the review of literature regarding the therapeutic values described from 200 BC to 20th century.

COMPREHENSIVE LITERATURE REVIEW OF MANDUKPARNI (CENTELLA ASIATICA) W.S.R. TO ITS MEDICINAL PROPERTIES

Ayurveda practitioners have managed and cured physical as well as mental health related disorders when there was no other stream of medicine evolved. The science of Ayurveda has evolved and emerged from ancient times to contemporary era as one of the most significant approach in medicine to treat various diseases. Ayurveda scholars from ancient times have invented and documented various medicinal herbs and their preparation and formulations to be used for the treatment. All Samhita Granths, Chikitsa Granths and Nighantus have quoted many such drugs with their specific uses and indications in particular diseases. Various Nighantus are rich source of knowledge regarding identification, place of origin and indications of various medicinal plants. Later on, with the evolution of technology, more researches were done on the medicinal plants and their utilities in specific disorders were re-evaluated. Mandukparni is one such drug which has been quoted many times in ancient texts as well as in various Nighantus and then more research work was done regarding the medicinal properties of the plants in different ailments. The herb is indicated as Medhya and Rasayana primarily by nearly all Ayurvedic texts including Nighantus. Mandukparni is named Centella asiatica in binomial system of nomenclature. Modern researches have also signified the use of this herb as a potent cognitive drug and having anti-oxidant properties. Alongside, the herb is indicated in many other diseases in ancient and modern texts. This article is an attempt to throw a light on the mentions, formulations and indications of this herb as described in all available texts and research works.

Undercooling studies on dispersed bismuth droplets

In the zinc-bismuth system, a monotectic reaction occurs at 689 K and 0·6 at.% Bi composition. Rapid solidification of the as-cast monotectic alloy led to a micromorphology in which bismuth was uniformly and bimodally distributed as small droplets in the zinc matrix. Statistical analysis of the electron micrographs obtained from different transparent regions of the foils revealed that the size of most of the droplets was about 6 nm. These droplets undercooled by 132 K. An analysis of the nucleation rate measurements shows that the activation energy barrier to nucleation is of the order of 39·8 kcal/mol at the maximum undercooling

Inhibitor of Sarco/Endoplasmic Reticulum Calcium-ATPase Impairs Multiple Steps of Paramyxovirus Replication

Sarco/endoplasmic reticulum calcium-ATPase (SERCA) is a membrane-bound cytosolic enzyme which is known to regulate the uptake of calcium into the sarco/endoplasmic reticulum. Herein, we demonstrate for the first time that SERCA can also regulate virus replication. Treatment of Vero cells with SERCA-specific inhibitor (Thapsigargin) at a concentration that is nontoxic to the cells significantly reduced Peste des petits ruminants virus (PPRV) and Newcastle disease virus (NDV) replication. Conversely, overexpression of SERCA rescued the inhibitory effect of Thapsigargin on virus replication. PPRV and NDV infection induced SERCA expression in Vero cells, which could be blocked by Thapsigargin. Besides inducing enhanced formation of cytoplasmic foci, Thapsigargin was shown to block viral entry into the target cells as well as synthesis of viral proteins. Furthermore, NDV was shown to acquire significant resistance to Thapsigargin upon long-term passage (P) in Vero cells. As compared to the P0 and P70-Control, the fusion (F) protein of P70-Thapsigargin virus exhibited a unique mutation at amino acid residue 104 (E104K), whereas no Thapsigargin-associated mutations were observed in HN gene. To the best of our knowledge, this is the first report describing the virus-supportive role of SERCA and a rare report suggesting that viruses may acquire resistance even in the presence of an inhibitor that targets a cellular factor

Endothelial NADPH oxidase-2 promotes interstitial cardiac fibrosis and diastolic dysfunction through proinflammatory effects and endothelial-mesenchymal transition

OBJECTIVES: This study sought to investigate the effect of endothelial dysfunction on the development of cardiac hypertrophy and fibrosis. BACKGROUND: Endothelial dysfunction accompanies cardiac hypertrophy and fibrosis, but its contribution to these conditions is unclear. Increased nicotinamide adenine dinucleotide phosphate oxidase-2 (NOX2) activation causes endothelial dysfunction. METHODS: Transgenic mice with endothelial-specific NOX2 overexpression (TG mice) and wild-type littermates received long-term angiotensin II (AngII) infusion (1.1 mg/kg/day, 2 weeks) to induce hypertrophy and fibrosis. RESULTS: TG mice had systolic hypertension and hypertrophy similar to those seen in wild-type mice but developed greater cardiac fibrosis and evidence of isolated left ventricular diastolic dysfunction (p < 0.05). TG myocardium had more inflammatory cells and VCAM-1-positive vessels than did wild-type myocardium after AngII treatment (both p < 0.05). TG microvascular endothelial cells (ECs) treated with AngII recruited 2-fold more leukocytes than did wild-type ECs in an in vitro adhesion assay (p < 0.05). However, inflammatory cell NOX2 per se was not essential for the profibrotic effects of AngII. TG showed a higher level of endothelial-mesenchymal transition (EMT) than did wild-type mice after AngII infusion. In cultured ECs treated with AngII, NOX2 enhanced EMT as assessed by the relative expression of fibroblast versus endothelial-specific markers. CONCLUSIONS: AngII-induced endothelial NOX2 activation has profound profibrotic effects in the heart in vivo that lead to a diastolic dysfunction phenotype. Endothelial NOX2 enhances EMT and has proinflammatory effects. This may be an important mechanism underlying cardiac fibrosis and diastolic dysfunction during increased renin-angiotensin activation

Nucleation kinetics studies on embedded nanosized indium (In) particles

241-246Indium exhibits a monotectic reaction with Al at an invariant temperature (910 K) and composition (4.7 at.% In). Ultra rapid cooling, using gun technique, of monotectic compositioned alloy led to a microstructure in which In was distributed uniformly with a bimodal size distribution. Quantitative metallographic analysis revealed size of small sized In particles to be in the range of 10-20 nm whereas bigger particles were found to be even greater than 200 nm in size. These particles of In undercooled by 19 K in the rapidly solidified product of Al-In system. In the as-cast condition the degree of undercooling was only 9 K. Analyses of the nucleation rate measurements showed that the activation energy barrier to nucleation is 2.666 kcal/mol at undercoolings approaching 19 K. The nucleus-substrate contact angle was found to be 20°, which signifies that Al provides a potent catalytic surface for the nucleation of In

Critical assessment of Flory’s approach for estimating miscibility gap in binary monotectic systems

364-366Flory’s  solution approach, which gives expression for the free energy of mixing of binary alloys whose constituent atoms differ largely in size, has been used to find the required model parameters in terms of which expressions for critical temperature (Tc) and critical composition  (Xc) have been derived. Model parameters are capable of describing the thermodynamic behavior of the liquid phase and are useful in constructing the miscibility gap in binary monotectics. Within the limits fairly good agreement has been observed between theoretically calculated and experimentally observed values of Xc  and Tc  along with the reasonable data for the various thermodynamic functions at the desired temperature in four binary monotectic systems chosen for the present study