5 research outputs found
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Implications of advanced wastewater treatment: Electrocoagulation and electroflocculation of effluent discharged from a wastewater treatment plant
In this research, wastewater treatment was inspected on a pilot-scale wastewater treatment plant by electrochemical techniques, electrocoagulation (EC), electroflotation (EF) and electrophoretic deposition (EPD). The wastewater samples have been characterised by applying different parameters to determine optimum working conditions of the electrocoagulation reactor. Two electrodes have been tested separately with an outflow coming from primary and secondary sedimentation tank. The outflows from these tanks are introduced in EC reactor then EC reactor efficacy is determined for the removal of chemical oxygen demand (COD), suspended solids, micropollutants and amount of coagulants in agglomerates at different current densities. The amounts of suspended solids (SS) in influent and effluent streams were determined by the membrane filtration technique. The operational applied current values range from 1–4 A in the case of COD removal by Fe and Al. While for SS aggregation the applied current ranges from 0.5–3 A and inflow rate was tested from 250 to 500 L/h. The pH of outflows increased by increasing applied current and both of these parameters were found a positive increase in the amount of SS aggregations after EC treatment. Furthermore, the COD removal efficiency was found to be 56–57 % and 12–18 % in case Fe and Al electrode respectively after EC treatment. The results showed that applied current is the most effective parameter, whereas the aluminium electrodes have produced more amounts of flocs and bubbles in comparison to iron electrodes at similar amount of current density
Synthesis of 5-Fluorouracil Cocrystals with Novel Organic Acids as Coformers and Anticancer Evaluation against HCT-116 Colorectal Cell Lines
5-Fluorouracil (5-FU) being a mainstream anticancer drug is under keen and detailed investigation for prodrugs formulations in order to minimize the associated side effects. Cocrystallization of 5-FU is an innovative technique for the synthesis of 5-FU prodrugs to improve its anticancer effectiveness. The present study is based on the synthesis of 5-FU supramolecular synthons with four coformers: succinic acid, cinnamic acid, malic acid, and benzoic acid utilizing acetone as a solvent. Solid state grinding followed by a slow evaporation solution method was applied. Colorless clear crystals were obtained in all the cases. The cocrystal formation was supported with the help of Fourier transform infrared (FTIR) spectroscopy and powder X-ray diffraction (PXRD). Through FTIR, the main peaks of interest in the spectrum of 5-FU were N–H (3409.02 cm–1) and carbonyl group (1647.80 cm–1), which were prominently shifted in all spectra of the cocrystals demonstrating the replacement as well as the development of already present interactions with the new ones. For 5-FU–cinnamic acid cocrystals, the anticipated peaks were observed at 1673.13 cm–1 (−C═O) and 3566.89 cm–1 (N–H) manifesting a significant change in comparison to 5-FU. Furthermore, with the help of PXRD characterization, the representative peak of 5-FU was recorded at 2θ = 28.80°. The shifting of this specific peak and development of many new ones in the spectra of cocrystals proved the development of new structural entities. Finally, the anticancer activity of all cocrystals was evaluated in comparison to that of API. All cocrystals manifest significantly greater growth inhibition potential than the main active pharmaceutical ingredient. 5-FU–Cinnamic acid (3C) was the one that proved to be the most potent anticancer agent at all four concentrations: 4.82% (12 μg/mL), 34.21% (25 μg/mL), 55.08% (50 μg/mL), and 67.29% (100 μg/mL). In short, this study proved to be a true example to enhance the anticancer potential of 5-FU following fairly easy fabrication requirements of the cocrystallization phenomenon. After the successful synthesis of these supramolecular synthons and subsequent enhancement of growth inhibition potential of 5-FU, these cocrystals can further be evaluated for in vivo trials and membrane crossing potentials in the future
Green synthesis and biological evaluation of novel 5-fluorouracil derivatives as potent anticancer agents
This study reports the formation of 5-FU co-crystals with four different pharmacologically safe co-formers; Urea, Thiourea, Acetanilide and Aspirin using methanol as a solvent. Two fabrication schemes were followed i.e., solid-state grinding protocol, in which API and co-formers were mixed through vigorous grinding while in the other method separate solutions of both the components were made and mixed together. The adopted approaches offer easy fabrication protocols, no temperature maintenance requirements, no need of expensive solvents, hardly available apparatus, isolation and purification of the desired products. In addition, there is no byproducts formation, In fact, a phenomenon embracing the requirements of green synthesis. Through FTIR analysis; for API the Nsingle bondH absorption frequency was recorded at 3409.02 cm−1 and that of single bondCdouble bondO was observed at 1647.77 cm−1. These characteristics peaks of 5-FU were significantly shifted and recorded at 3499.40 cm−1 and 1649.62 cm−1 for 5-FU-Ac (3B) and 3496.39 cm−1 and 1659.30 cm−1 for 5-FU-As (4B) co-crystals for Nsingle bondH and single bondCdouble bondO groups respectively. The structural differences between API and co-crystals were further confirmed through PXRD analysis. The characteristic peak of 5-FU at 2θ = 28.79918o was significantly shifted in the graphs of co-crystals not only in position but also with respect to intensity and FWHM values. In addition, new peaks were also recorded in all the spectra of co-formers confirming the structural differences between API and co-formers. In addition, percent growth inhibition was also observed by all the co-crystals through MTT assay against HCT 116 colorectal cell lines in vitro. At four different concentrations; 25, 50, 100 and 200 µg/mL, slightly different trends of the effectiveness of API and co-crystals were observed. However; among all the co-crystal forms, 5-FU-thiourea co-crystals obtained through solution method (2B) proved to be the most effective growth inhibitor at all the four above mentioned concentrations
Development of biomass derived highly porous fast adsorbents for post-combustion CO2 capture
This study is carried out for a comparative screening of three groups of biomasses; soft or non-woody (peanut shell); intermediate woody (walnut shell) and hard woody (pine wood) for the development of adsorbents/activated carbons for post-combustion CO2 capture (over N2 balance). Three different groups of biomass residues are selected to study the role and nature of the material in adsorption and selection of the raw material for CO2 adsorbents synthesis for future researches because of the hot issue of anthropogenic CO2 emissions. The adsorption isotherms studied by the thermal gravimetric analyser (TGA) revealed that CO2 adsorption capabilities are in the range of 2.53–3.92 mmol/g (over N2 balance) at 25 °C. The newly synthesised activated carbons (ACs) exhibited a fast rate of adsorption as 41–94% in the initial 2 min. Porous surface development with catalytic KOH activation is seen clearly through SEM surface morphological analyses and mathematically confirmed from SBET ranges from 146.86 to 944.05 m2/g. FTIR and XRD peaks verify the generation of basic or inorganic O2-rich moieties that help in acidic CO2 capture. It has also been observed from adsorption isotherms that the order of higher adsorption groups is as; peanut shell > pine wood > walnut shell, while the best activation mass ratio (sample/KOH) is 1:3. The synthesised low cost ACs with an amount of 1.93 US$ per kg production could help to overcome the environmental hazards and problems caused by CO2 and biomass waste
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Trackable CEMB-Klean Cotton Transgenic Technology: Afforadable Climate Neutral Agri-biotech Industrialization for Developing Countries
Background: Transgenic technology reflects the incorporation of novel useful traits in crop plants like cotton for economic benefits by overcoming the problems including insects' pests and weeds in special. The present study is the success story of the continuous effort of CEMB team started back in the 1990s. Methods: This study includes characterization of a large number of Bacillus thuringiensis (Bt) strains taken from local soil and subjected to direct transformation of isolated BT genes into local cotton cultivars. Protocols for transformation into cotton plants were optimized and validated by the development of double gene codon optimized (Cry1Ac and Cry2A) transgenic cotton varieties. Results: The resulting GMOs in the form of CEMB-33, CA-12, CEMB-66 have been approved by Punjab Seed Council in 2013 and 2016 respectively. Double Bt and weedicide resistant cotton harboring CEMB-Modified and codon optimized cp4EPSPS (GTGene). These varieties can tolerate glyphosate spray @ 1900ml per acre without the appearance of necrotic spots/shedding and complete removal of all surrounding weeds in the cotton field is a significant advance to boost cotton production without spending much on insecticides and herbicides. Conclusion: In the current report, two unique sets of primers which amplify 1.1 Kb for CEMB-double Bt genes and 660 bp product for CEMB-Modified cp4EPSPS (GTGene) were tested. CEMB cotton variety CKC-01 is specially designed as low cost and easy to use by local farmer's technology has the potential to revolutionize the cotton growing culture of the country.Higher Education Commission (HEC) of PakistanOpen access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]