Hemolytic and DNA binding studies of divalent transition metal ion based macrocyclic complexes

529-535New tetra-azamacrocyclic complexes of Ni (II) and Cu(II) have been synthesized by template methodology leading to the formation of complex of type [MLX2] where L is a macrcocyclic ligand obtained from the condensation of 4-Methyl-o-phenylenediamine (DAT) and 1,3-diphenylpropane-1,3-dione (DBM)and X= NO3-, Cl-, and CH3COO-. Characterization of newly prepared complexes has been  done by using various physico-analytical techniques like UV-visible, IR, ESR, CHN, Magnetic susceptibilities and PXRD.The non-electrolytic nature of the complexes was elucidated by lower value of molar conductance. The data received from various techniques give an indication towards the octahedral geometry of the complexes. The macrocyclic ring is present at the equatorial position whereas the axial positions are occupied by the ligands Cl-, NO3- and CH3COO-. Screening of all the complexes has been performed against the pathogenic strains of microbes in order to check their antimicrobial potential. Invitro-hemolytic activity reveals about the extent to which lyses of hemoglobin takes place. The Herring fish sperm DNA interaction studies are carried out with the help of UV-absorption spectra. Molecular modeling was done through the assistance of  software Chem 3D Ultra that gives the energy calculation and their quantum chemical parameter

Primary Fallopian Tube Cancer - An Incidental Finding in a Young Patient

No Abstract

Reconstructing an ancestral genotype of two hexachlorocyclohexane-degrading Sphingobium species using metagenomic sequence data.

Over the last 60 years, the use of hexachlorocyclohexane (HCH) as a pesticide has resulted in the production of >4 million tons of HCH waste, which has been dumped in open sinks across the globe. Here, the combination of the genomes of two genetic subspecies (Sphingobium japonicum UT26 and Sphingobium indicum B90A; isolated from two discrete geographical locations, Japan and India, respectively) capable of degrading HCH, with metagenomic data from an HCH dumpsite (∼450 mg HCH per g soil), enabled the reconstruction and validation of the last-common ancestor (LCA) genotype. Mapping the LCA genotype (3128 genes) to the subspecies genomes demonstrated that >20% of the genes in each subspecies were absent in the LCA. This includes two enzymes from the 'upper' HCH degradation pathway, suggesting that the ancestor was unable to degrade HCH isomers, but descendants acquired lin genes by transposon-mediated lateral gene transfer. In addition, anthranilate and homogentisate degradation traits were found to be strain (selectively retained only by UT26) and environment (absent in the LCA and subspecies, but prevalent in the metagenome) specific, respectively. One draft secondary chromosome, two near complete plasmids and eight complete lin transposons were assembled from the metagenomic DNA. Collectively, these results reinforce the elastic nature of the genus Sphingobium, and describe the evolutionary acquisition mechanism of a xenobiotic degradation phenotype in response to environmental pollution. This also demonstrates for the first time the use of metagenomic data in ancestral genotype reconstruction, highlighting its potential to provide significant insight into the development of such phenotypes

Management of Rust in Pearl millet caused by Puccinia substriata var. penicillariae using Plant Product, Bioagent and Fungicides

Rust caused by Puccinia substriata var. penicillariae is one of the major disease affecting both forage and grain production in pearl millet. An attempt was made to manage pearl millet rust using plant product, bioagent and fungicides under screen house and field conditions. The experiment was conducted on susceptible hybrid HHB 197 both under screen house and field condition with eight treatments. Observation on rust severity recorded at grain filling stage. The experiment results indicated that all the treatments were effective in managing the disease but amongst them minimum disease severity (11.7%) and (21.7%) was contracted under screen house and field conditions respectively in treatment of Propiconazole 25% EC (0.1%) followed by Hexaconazole 5% EC (0.1%) and Copper oxychloride 50% WP (0.2%), Carbendazim 50% WP (0.2%), Mancozeb 75% WP (0.2%), Azadirachtin 0.15% (1500 ppm), Trichoderma viride (3%) treated pots and plot. Maximum grain yield (514.7 kg/acre), test weight (8.13 g) and Benefit: cost 3.98: 1 was observed in Propiconazole 25% EC (0.1%) sprayed plot followed by Hexaconazole 5% EC (0.1%) under field conditions

To study the outcome of previous one cesarean pregnancies in a rural tertiary center of Haryana, India

Background: Rising rates of caesarean section is a matter of great concern and TOLAC is an attractive alternative. Analysing outcome of previous one caesarean pregnancies will provide an insight for reducing the caesarean rates and formulating protocols and policies for TOLAC.Methods: A retrospective study of patients of previous one caesarean pregnancy was done from February 2015 to January 2016 and 3 groups were made, ERCS group, failed TOLAC group and successful TOLAC group. The rates of elective repeat caesarean, failed TOLAC, successful TOLAC, maternal complications, neonatal morbidity and mortality in all three groups were studied.Results: There were 5177 total deliveries with 488 (9.43%) previous one caesarean pregnancies. Out of 488 patients 161 (33%) underwent elective repeat caesarean and 327 (67%) underwent trial of labour. Out of 327 patients 234 (71.56%) had a successful TOLAC and 93 (28.44%) had failed TOLAC. Breech (23%) followed by foetal distress (20%) were the most common indications of previous caesarean. Commonest indication of elective repeat caesarean was short interval (33%) and that of failed TOLAC was foetal distress (38.7%) followed by failed induction (23.6%). There were 4 morbidly adherent placentas (0.82%), 1 scar rupture, 3 scar dehiscence, no maternal mortality and 10 neonatal deaths.Conclusions: Previous one caesarean section is not only a risk factor for repeat caesareans and complications like morbidly adherent placenta, uterine rupture but also a financial burden on health facilities. Encouraging the patients for trial of labour and emphasizing the usage of contraception is the need of the hour